ジャーナル: Protein Cell / 年: 2014 タイトル: Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence. 著者: Zhixiu Yang / Qiang Guo / Simon Goto / Yuling Chen / Ningning Li / Kaige Yan / Yixiao Zhang / Akira Muto / Haiteng Deng / Hyouta Himeno / Jianlin Lei / Ning Gao / 要旨: The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA ...The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coli strain (∆rsgA∆rbfA). The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, intermediates derived under the two contrasting salt conditions (high and low) likely reflect two distinctive assembly stages, the relatively early and late stages of the 3' domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5' end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal the location of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mechanisms on subunit production and protein translation.
ダウンロード / ファイル: emd_5910.map.gz / 形式: CCP4 / 大きさ: 7.5 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
the map is normalized to N(0,1)
ボクセルのサイズ
X=Y=Z: 2.62 Å
密度
表面レベル
登録者による: 2.73 / ムービー #1: 2.73
最小 - 最大
-3.56118298 - 13.224247930000001
平均 (標準偏差)
0.0 (±0.9999997)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-62
-62
-62
サイズ
126
126
126
Spacing
126
126
126
セル
A=B=C: 330.12 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.62
2.62
2.62
M x/y/z
126
126
126
origin x/y/z
0.000
0.000
0.000
length x/y/z
330.120
330.120
330.120
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-95
-75
153
NX/NY/NZ
200
200
200
MAP C/R/S
1
2
3
start NC/NR/NS
-62
-62
-62
NC/NR/NS
126
126
126
D min/max/mean
-3.561
13.224
-0.000
-
添付データ
-
試料の構成要素
-
全体 : Cryo-EM structure of high salt treated immature 30S ribosomal sub...
全体
名称: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain
要素
試料: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain
複合体: high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain
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超分子 #1000: Cryo-EM structure of high salt treated immature 30S ribosomal sub...
超分子
名称: Cryo-EM structure of high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain タイプ: sample / ID: 1000 / Number unique components: 1
分子量
実験値: 800 KDa / 理論値: 800 KDa
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超分子 #1: high salt treated immature 30S ribosomal subunit from rsga and rb...
超分子
名称: high salt treated immature 30S ribosomal subunit from rsga and rbfa deleted E.coli strain タイプ: complex / ID: 1 / Name.synonym: immature 30S / 組換発現: No / データベース: NCBI / Ribosome-details: ribosome-prokaryote: SSU 30S