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- EMDB-5717: The structure of Sinorhizobium meliloti phage phiM12, a novel T=1... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-5717 | |||||||||
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Title | The structure of Sinorhizobium meliloti phage phiM12, a novel T=19 icosahedral phage that is the founder of a new group of T4-like phages | |||||||||
![]() | Cryo-EM reconstruction of phiM12, packed with DNA | |||||||||
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![]() | bacteriophage / phiM12 / Sinorhizobium meliloti / T=19 / rhizobia | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 13.0 Å | |||||||||
![]() | Stroupe ME / Brewer TE / Sousa DR / Jones KM | |||||||||
![]() | ![]() Title: The structure of Sinorhizobium meliloti phage ΦM12, which has a novel T=19l triangulation number and is the founder of a new group of T4-superfamily phages. Authors: M Elizabeth Stroupe / Tess E Brewer / Duncan R Sousa / Kathryn M Jones / ![]() Abstract: ΦM12 is the first example of a T=19l geometry capsid, encapsulating the recently sequenced genome. Here, we present structures determined by cryo-EM of full and empty capsids. The structure reveals ...ΦM12 is the first example of a T=19l geometry capsid, encapsulating the recently sequenced genome. Here, we present structures determined by cryo-EM of full and empty capsids. The structure reveals the pattern for assembly of 1140 HK97-like capsid proteins, pointing to interactions at the pseudo 3-fold symmetry axes that hold together the asymmetric unit. The particular smooth surface of the capsid, along with a lack of accessory coat proteins encoded by the genome, suggest that this interface is the primary mechanism for capsid assembly. Two-dimensional averages of the tail, including the neck and baseplate, reveal that ΦM12 has a relatively narrow neck that attaches the tail to the capsid, as well as a three-layer baseplate. When free from DNA, the icosahedral edges expand by about 5nm, while the vertices stay at the same position, forming a similarly smooth, but bowed, T=19l icosahedral capsid. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 18.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.3 KB 9.3 KB | Display Display | ![]() |
Images | ![]() ![]() | 74.6 KB 4.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 78.7 KB | Display | ![]() |
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Full document | ![]() | 77.8 KB | Display | |
Data in XML | ![]() | 493 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM reconstruction of phiM12, packed with DNA | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 5.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : T=19 icosahedral shell packed with DNA from phiM12, a bacteriopha...
Entire | Name: T=19 icosahedral shell packed with DNA from phiM12, a bacteriophage of Sinorhizobium meliloti |
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Components |
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-Supramolecule #1000: T=19 icosahedral shell packed with DNA from phiM12, a bacteriopha...
Supramolecule | Name: T=19 icosahedral shell packed with DNA from phiM12, a bacteriophage of Sinorhizobium meliloti type: sample / ID: 1000 / Oligomeric state: icosahedral / Number unique components: 2 |
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-Supramolecule #1: Sinorhizobium phage phiM12
Supramolecule | Name: Sinorhizobium phage phiM12 / type: virus / ID: 1 / NCBI-ID: 1357423 / Sci species name: Sinorhizobium phage phiM12 / Sci species strain: phiM12 / Database: NCBI / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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Host (natural) | Organism: ![]() |
Virus shell | Shell ID: 1 / Name: 1 / Diameter: 1000 Å / T number (triangulation number): 19 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 / Details: 10 mM Na2HPO4, 1 mM MgSO4 |
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Grid | Details: 200 mesh 2/2 Quantifoil |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 120 K / Instrument: FEI VITROBOT MARK IV / Method: blot 4 seconds |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Date | May 13, 2013 |
Image recording | Category: CCD / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Number real images: 1000 / Average electron dose: 15 e/Å2 |
Tilt angle min | 0 |
Tilt angle max | 0 |
Electron beam | Acceleration voltage: 120 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 65555 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 59000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Details | Images were selected by hand; initial model determined in EMAN and refined in EMAN and Frealign. |
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CTF correction | Details: micrograph |
Final reconstruction | Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 13.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: EMAN, FREALIGN / Number images used: 2038 |