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- EMDB-5561: Cryo EM map of the Gaalphaq-PLCbeta3 complex. -

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Basic information

Entry
Database: EMDB / ID: EMD-5561
TitleCryo EM map of the Gaalphaq-PLCbeta3 complex.
Map dataReconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
Sample
  • Sample: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
  • Protein or peptide: G alpha q
  • Protein or peptide: phospholipase C beta 3
KeywordsGTP-binding protein alpha subunits / phospholipase C beta / coiled-coil domain / PH DOMAIN / EF HAND / C2 DOMAIN / TIM BARREL DOMAIN / phospholipase / GTP hydrolysis / G-protein signaling / membrane targeting / lipase / hydrolase / calcium binding / GTP binding / phospholipids / GTP-BINDING PROTEIN-HYDROLASE complex
Function / homologyPhosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta / G-protein alpha subunit, group Q / activation of phospholipase C activity / G protein-coupled receptor signaling pathway
Function and homology information
Biological speciesMus musculus (house mouse) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 19.0 Å
AuthorsDutta S / Lyon AM / Tesmer JJG / Skiniotis G
CitationJournal: Nat Struct Mol Biol / Year: 2013
Title: Full-length Gα(q)-phospholipase C-β3 structure reveals interfaces of the C-terminal coiled-coil domain.
Authors: Angeline M Lyon / Somnath Dutta / Cassandra A Boguth / Georgios Skiniotis / John J G Tesmer /
Abstract: Phospholipase C-β (PLCβ) is directly activated by Gαq, but the molecular basis for how its distal C-terminal domain (CTD) contributes to maximal activity is poorly understood. Herein we present ...Phospholipase C-β (PLCβ) is directly activated by Gαq, but the molecular basis for how its distal C-terminal domain (CTD) contributes to maximal activity is poorly understood. Herein we present both the crystal structure and cryo-EM three-dimensional reconstructions of human full-length PLCβ3 in complex with mouse Gαq. The distal CTD forms an extended monomeric helical bundle consisting of three antiparallel segments with structural similarity to membrane-binding bin-amphiphysin-Rvs (BAR) domains. Sequence conservation of the distal CTD suggests putative membrane and protein interaction sites, the latter of which bind the N-terminal helix of Gαq in both the crystal structure and cryo-EM reconstructions. Functional analysis suggests that the distal CTD has roles in membrane targeting and in optimizing the orientation of the catalytic core at the membrane for maximal rates of lipid hydrolysis.
History
DepositionJan 3, 2013-
Header (metadata) releaseJan 23, 2013-
Map releaseMar 20, 2013-
UpdateApr 17, 2013-
Current statusApr 17, 2013Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 2.5
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 2.5
  • Imaged by UCSF Chimera
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_5561_1.jpg

Downloads & links

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Map

FileDownload / File: emd_5561.map.gz / Format: CCP4 / Size: 1001 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
Voxel sizeX=Y=Z: 4.48 Å
Density
Contour LevelBy AUTHOR: 2.5 / Movie #1: 2.5
Minimum - Maximum-6.21406794 - 18.742422099999999
Average (Standard dev.)0.00089894 (±1.2658869)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-32-32-32
Dimensions646464
Spacing646464
CellA=B=C: 286.72 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.484.484.48
M x/y/z646464
origin x/y/z0.0000.0000.000
length x/y/z286.720286.720286.720
α/β/γ90.00090.00090.000
start NX/NY/NZ-5029166
NX/NY/NZ106122134
MAP C/R/S123
start NC/NR/NS-32-32-32
NC/NR/NS646464
D min/max/mean-6.21418.7420.001

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Supplemental data

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Sample components

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Entire : Reconstruction of the PLCbeta3 distal CTD in complex with the N-t...

EntireName: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
Components
  • Sample: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
  • Protein or peptide: G alpha q
  • Protein or peptide: phospholipase C beta 3

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Supramolecule #1000: Reconstruction of the PLCbeta3 distal CTD in complex with the N-t...

SupramoleculeName: Reconstruction of the PLCbeta3 distal CTD in complex with the N-terminal helix of Galphaq
type: sample / ID: 1000
Details: The sample was monodisperse and had a molecular weight consistent with the theoretical weight.
Oligomeric state: Galphaq-PLCb3 dimer / Number unique components: 3
Molecular weightExperimental: 1.8 MDa / Theoretical: 1.8 MDa
Method: Size exclusion chromatography and multi-angle light scattering

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Macromolecule #1: G alpha q

MacromoleculeName: G alpha q / type: protein_or_peptide / ID: 1 / Name.synonym: G a q / Number of copies: 1 / Oligomeric state: monomer / Recombinant expression: Yes
Source (natural)Organism: Mus musculus (house mouse) / synonym: mouse / Location in cell: cytosol and plasma membrane
Molecular weightExperimental: 44 KDa / Theoretical: 44 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper) / Recombinant plasmid: pFastBacHTA
SequenceGO: activation of phospholipase C activity / InterPro: G-protein alpha subunit, group Q

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Macromolecule #2: phospholipase C beta 3

MacromoleculeName: phospholipase C beta 3 / type: protein_or_peptide / ID: 2 / Name.synonym: PLCbeta3 / Number of copies: 1 / Oligomeric state: monomer / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human) / synonym: Human / Location in cell: cytosol and plasma membrane
Molecular weightExperimental: 139 KDa / Theoretical: 139 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper) / Recombinant plasmid: pFastBacDual
SequenceGO: G protein-coupled receptor signaling pathway
InterPro: Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 8
Details: 20 mM HEPES, pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 uM GDP, 30 uM AlCl3, 10 mM NaF, 5 mM MgCl2
GridDetails: glow-discharged Quantifoil R2/200 mesh grid
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV
Details: Vitrification carried out in liquid nitrogen atmosphere.
Method: Blot 3 microliter sample for 2 seconds.

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Electron microscopy

MicroscopeFEI TECNAI F20
Electron beamAcceleration voltage: 120 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 71138 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 50000
Sample stageSpecimen holder: Used two holders. / Specimen holder model: OTHER
TemperatureMin: 89 K / Max: 95 K / Average: 89 K
Alignment procedureLegacy - Astigmatism: Objective lens astigmatism was corrected at 135,000 times magnification.
DateOct 20, 2011
Image recordingCategory: CCD / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Number real images: 350 / Average electron dose: 20 e/Å2
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

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Image processing

CTF correctionDetails: each particle
Final reconstructionAlgorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 19.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: EMAN1 / Number images used: 12692

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Atomic model buiding 1

Initial modelPDB ID:

4gnk

RefinementSpace: REAL

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