EMDB-52619: High resolution Cryo-EM structure of human complex I in mitochondria

Basic information

Entry

Database: EMDB / ID: EMD-52619

• Title: High resolution Cryo-EM structure of human complex I in mitochondria

Sample

• Cell: Complex I in human mitochondria
  • Protein or peptide: x 44 types
• Ligand: x 12 types

• Keywords: Human mitochondria / respirasome complex / complex I / NADH dehydrogenase / MEMBRANE PROTEIN

Function / homology

Function:

protein lipoylation / Complex I biogenesis / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / Respiratory electron transport / protein insertion into mitochondrial inner membrane / ubiquinone biosynthetic process / blastocyst hatching / cellular respiration / cellular response to oxygen levels / response to light intensity / mesenchymal stem cell proliferation / Mitochondrial protein import / reproductive system development / iron-sulfur cluster assembly complex / respiratory chain complex / mitochondrial [2Fe-2S] assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / mesenchymal stem cell differentiation / circulatory system development / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / negative regulation of non-canonical NF-kappaB signal transduction / cardiac muscle tissue development / neural precursor cell proliferation / [2Fe-2S] cluster assembly / oxygen sensor activity / positive regulation of mitochondrial membrane potential / response to hydroperoxide / azurophil granule membrane / cellular response to glucocorticoid stimulus / stem cell division / NADH dehydrogenase activity / iron-sulfur cluster assembly / sodium ion transport / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial ATP synthesis coupled electron transport / regulation of protein phosphorylation / positive regulation of ATP biosynthetic process / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / proton motive force-driven mitochondrial ATP synthesis / RHOG GTPase cycle / respiratory chain complex I / response to cAMP / positive regulation of execution phase of apoptosis / NADH dehydrogenase (ubiquinone) activity / endopeptidase activator activity / quinone binding / cellular response to interferon-beta / negative regulation of reactive oxygen species biosynthetic process / extrinsic apoptotic signaling pathway / cellular response to retinoic acid / neurogenesis / ionotropic glutamate receptor binding / Mitochondrial protein degradation / substantia nigra development / muscle contraction / reactive oxygen species metabolic process / aerobic respiration / synaptic membrane / cerebellum development / fatty acid binding / regulation of mitochondrial membrane potential / respiratory electron transport chain / response to nicotine / DNA damage response, signal transduction by p53 class mediator / kidney development / response to hydrogen peroxide / monooxygenase activity / sensory perception of sound / fatty acid metabolic process / circadian rhythm / brain development / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / multicellular organism growth / NAD binding / positive regulation of protein catabolic process / fatty acid biosynthetic process / cellular senescence / FMN binding / nervous system development / 4 iron, 4 sulfur cluster binding / response to oxidative stress / protease binding / response to ethanol / gene expression / in utero embryonic development / response to hypoxia / electron transfer activity / mitochondrial inner membrane / nuclear speck / nuclear body / mitochondrial matrix

Domain/homology:

Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / : / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH-ubiquinone oxidoreductase MWFE subunit / : / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / : / SLBB domain / : / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone oxidoreductase chain 4L/K / NADH-quinone oxidoreductase, chain M/4 / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH ubiquinone oxidoreductase, F subunit / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2.

Component:

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.63 Å
• Authors: Nguyen MD / Singh V / Rorbach J

Funding support

Sweden, 1 items

Organization	Grant number	Country
Knut and Alice Wallenberg Foundation		Sweden

• Citation: Journal: To Be Published; Title: Structural basis for late maturation steps of mitochondrial respiratory chain complex IV within the human respirasome; Authors: Nguyen MD / Singh V / Rorbach J

History

Deposition	Jan 24, 2025	-
Header (metadata) release	Nov 12, 2025	-
Map release	Nov 12, 2025	-
Update	Nov 12, 2025	-
Current status	Nov 12, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_52619.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 0.875 Å

Density

Contour Level	By AUTHOR: 0.477
Minimum - Maximum	-1.294451 - 4.38072
Average (Standard dev.)	0.00092549674 (±0.10893269)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 420.0 Å α=β=γ: 90.0 °

Supplemental data

Half map: #1

• File: emd_52619_half_map_1.map

Half map: #2

• File: emd_52619_half_map_2.map

Sample components

Entire : Complex I in human mitochondria

• Entire: Name: Complex I in human mitochondria

Components

• Cell: Complex I in human mitochondria
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
  • Protein or peptide: Acyl carrier protein, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
• Ligand: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
• Ligand: CARDIOLIPIN
• Ligand: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate
• Ligand: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL
• Ligand: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: IRON/SULFUR CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Complex I in human mitochondria

• Supramolecule: Name: Complex I in human mitochondria / type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.626559 KDa

Sequence

String:

MAALRALCGF RGVAAQVLRP GAGVRLPIQP SRGVRQWQPD VEWAQQFGGA VMYPSKETAH WKPPPWNDVD PPKDTIVKNI TLNFGPQHP AAHGVLRLVM ELSGEMVRKC DPHIGLLH(NMM)G TEKLIEYKTY LQALPYFDRL DYVSMMCNEQ AYSLAVE KL LNIRPPPRAQ WIRVLFGEIT RLLNHIMAVT THALDLGAMT PFFWLFEERE KMFEFYERVS GARMHAAYIR PGGVHQDL P LGLMDDIYQF SKNFSLRLDE LEELLTNNRI WRNRTIDIGV VTAEEALNYG FSGVMLRGSG IQWDLRKTQP YDVYDQVEF DVPVGSRGDC YDRYLCRVEE MRQSLRIIAQ CLNKMPPGEI KVDDAKVSPP KRAEMKTSME SLIHHFKLYT EGYQVPPGAT YTAIEAPKG EFGVYLVSDG SSRPYRCKIK APGFAHLAGL DKMSKGHMLA DVVAIIGTQD IVFGEVDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Macromolecule #2: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.084391 KDa

Sequence

String:

MWFEILPGLS VMGVCLLIPG LATAYIHRFT NGGKEKRVAH FGYHWSLMER DRRISGVDRY YVSKGLENID

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #3: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.287797 KDa

Sequence

String:

MAARVGAFLK NAWDKEPVLV VSFVVGGLAV ILPPLSPYFK YSVMINKATP YNYPVPVRDD GNMPDVPSHP QDPQGPSLEW LKKL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Macromolecule #4: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.86805 KDa

Sequence

String:

MAPKVFRQYW DIPDGTDCHR KAYSTTSIAS VAGLTAAAYR VTLNPPGTFL EGVAKVGQYT FTAAAVGAVF GLTTCISAHV REKPDDPLN YFLGGCAGGL TLGARTHNYG IGAAACVYFG IAASLVKMGR LEGWEVFAKP KV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Macromolecule #5: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.721311 KDa

Sequence

String:

MAASKVKQDM PPPGGYGPID YKRNLPRRGL SGYSMLAIGI GTLIYGHWSI MKWNRERRRL QIEDFEARIA LLPLLQAETD RRTLQMLRE NLEEEAIIMK DVPDWKVGES VFHTTRWVPP LIGELYGLRT TEEALHASHG FMWYT

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #6: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.434273 KDa

Sequence

String:

MASRVLSAYV SRLPAAFAPL PRVRMLAVAR PLSTALCSAG TQTRLGTLQP ALVLAQVPGR VTQLCRQYSD MPPLTLEGIQ DRVLYVLKL YDKIDPEKLS VNSHFMKDLG LDSLDQVEII MAMEDEFGFE IPDIDAEKLM CPQEIVDYIA DKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #7: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.072403 KDa

Sequence

String:

MSALTRLASF ARVGGRLFRS GCARTAGDGG VRHAGGGVHI EPRYRQFPQL TRSQVFQSEF FSGLMWFWIL WRFWHDSEEV LGHFPYPDP SQWTDEELGI PPDDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

Macromolecule #8: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.425006 KDa

Sequence

String:

MAHEHGHEHG HHKMELPDYR QWKIEGTPLE TIQKKLAAKG LRDPWGRNEA WRYMGGFAKS VSFSDVFFKG FKWGFAAFVV AVGAEYYLE SLNKDKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Macromolecule #9: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.784285 KDa

Sequence

String:

MAAMSLLRRV SVTAVAALSG RPLGTRLGFG GFLTRGFPKA AAPVRHSGDH GKRLFVIRPS RFYDRRFLKL LRFYIALTGI PVAIFITLV NVFIGQAELA EIPEGYVPEH WEYYKHPISR WIARNFYDSP EKIYERTMAV LQIEAEKAEL RVKELEVRKL M HVRGDGPW YYYETIDKEL IDHSPKATPD N

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Macromolecule #10: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.517228 KDa

Sequence

String:

MTGYTPDEKL RLQQLRELRR RWLKDQELSP REPVLPPQKM GPMEKFWNKF LENKSPWRKM VHGVYKKSIF VFTHVLVPVW IIHYYMKYH VSEKPYGIVE KKSRIFPGDT ILETGEVIPP MKEFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Macromolecule #11: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.791744 KDa

Sequence

String:

MAVARAGVLG VQWLQRASRN VMPLGARTAS HMTKDMFPGP YPRTPEERAA AAKKYNMRVE DYEPYPDDGM GYGDYPKLPD RSQHERDPW YSWDQPGLRL NWGEPMHWHL DMYNRNRVDT SPTPVSWHVM CMQLFGFLAF MIFMCWVGDV YPVYQPVGPK Q YPYNNLYL ERGGDPSKEP ERVVHYEI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Macromolecule #12: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.808729 KDa

Sequence

String:

MPDSWDKDVY PEPPRRTPVQ PNPIVYMMKA FDLIVDRPVT LVREFIERQH AKNRYYYYHR QYRRVPDITE CKEEDIMCMY EAEMQWKRD YKVDQEIINI MQDRLKACQQ REGQNYQQNC IKEVEQFTQV AKAYQDRYQD LGAYSSARKC LAKQRQRMLQ E RKAAKEAA AATS

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Macromolecule #13: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.336617 KDa

Sequence

String:

MAAGLFGLSA RRLLAAAATR GLPAARVRWE SSFSRTVVAP SAVAGKRPPE PTTPWQEDPE PEDENLYEKN PDSHGYDKDP VLDVWNMRL VFFFGVSIIL VLGSTFVAYL PDYRMKEWSR REAERLVKYR EANGLPIMES NCFDPSKIQL PEDE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Macromolecule #14: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.747248 KDa

Sequence

String:

MAPSALLRPL SRLLAPARLP SGPSVRSKFY VREPPNAKPD WLKVGFTLGT TVFLWIYLIK QHNEDILEYK RRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Macromolecule #15: NADH dehydrogenase [ubiquinone] 1 subunit C2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.209521 KDa

Sequence

String:

MIARRNPEPL RFLPDEARSL PPPKLTDPRL LYIGFLGYCS GLIDNLIRRR PIATAGLHRQ LLYITAFFFA GYYLVKREDY LYAVRDREM FGYMKLHPED FPEEDKKTYG EIFEKFHPIR

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

Macromolecule #16: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.542563 KDa

Sequence

String:

MPFLDIQKRF GLNIDRWLTI QSGEQPYKMA GRCHAFEKEW IECAHGIGYT RAEKECKIEY DDFVECLLRQ KTMRRAGTIR KQRDKLIKE GKYTPPPHHI GKGEPRP

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Macromolecule #17: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 38.980633 KDa

Sequence

String:

MNPLAQPVIY STIFAGTLIT ALSSHWFFTW VGLEMNMLAF IPVLTKKMNP RSTEAAIKYF LTQATASMIL LMAILFNNML SGQWTMTNT TNQYSSLMIM MAMAMKLGMA PFHFWVPEVT QGTPLTSGLL LLTWQKLAPI SIMYQISPSL NVSLLLTLSI L SIMAGSWG GLNQTQLRKI LAYSSITHMG WMMAVLPYNP NMTILNLTIY IILTTTAFLL LNLNSSTTTL LLSRTWNKLT WL TPLIPST LLSLGGLPPL TGFLPKWAII EEFTKNNSLI IPTIMATITL LNLYFYLRLI YSTSITLLPM SNNVKMKWQF EHT KPTPFL PTLIALTTLL LPISPFMLMI L

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

Macromolecule #18: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.189951 KDa

Sequence

String:

MNFALILMIN TLLALLLMII TFWLPQLNGY MEKSTPYECG FDPMSPARVP FSMKFFLVAI TFLLFDLEIA LLLPLPWALQ TTNLPLMVM SSLLLIIILA LSLAYEWLQK GLDWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #19: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.74511 KDa

Sequence

String:

MPLIYMNIML AFTISLLGML VYRSHLMSSL LCLEGMMLSL FIMATLMTLN THSLLANIVP IAMLVFAACE AAVGLALLVS ISNTYGLDY VHNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

Macromolecule #20: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 67.06893 KDa

Sequence

String:

MTMHTTMTTL TLTSLIPPIL TTLVNPNKKN SYPHYVKSIV ASTFIISLFP TTMFMCLDQE VIISNWHWAT TQTTQLSLSF KLDYFSMMF IPVALFVTWS IMEFSLWYMN SDPNINQFFK YLLIFLITML ILVTANNLFQ LFIGWEGVGI MSFLLISWWY A RADANTAA IQAILYNRIG DIGFILALAW FILHSNSWDP QQMALLNANP SLTPLLGLLL AAAGKSAQLG LHPWLPSAME GP TPVSALL HSSTMVVAGI FLLIRFHPLA ENSPLIQTLT LCLGAITTLF AAVCALTQND IKKIVAFSTS SQLGLMMVTI GIN QPHLAF LHICTHAFFK AMLFMCSGSI IHNLNNEQDI RKMGGLLKTM PLTSTSLTIG SLALAGMPFL TGFYSKDHII ETAN MSYTN AWALSITLIA TSLTSAYSTR MILLTLTGQP RFPTLTNINE NNPTLLNPIK RLAAGSLFAG FLITNNISPA SPFQT TIPL YLKLTALAVT FLGLLTALDL NYLTNKLKMK SPLCTFYFSN MLGFYPSITH RTIPYLGLLT SQNLPLLLLD LTWLEK LLP KTISQHQIST SIITSTQKGM IKLYFLSFFF PLILTLLLIT

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

Macromolecule #21: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 18.626963 KDa

Sequence

String:

MMYALFLLSV GLVMGFVGFS SKPSPIYGGL VLIVSGVVGC VIILNFGGGY MGLMVFLIYL GGMMVVFGYT TAMAIEEYPE AWGSGVEVL VSVLVGLAME VGLVLWVKEY DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT L FVGVYIVI EIARGN

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 6.97221 KDa

Sequence

String:

MVNLLQIVRD HWVHVLVPMG FVIGCYLDRK SDERLTAFRN KSMLFKRELQ PSEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Macromolecule #23: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.231596 KDa

Sequence

String:

MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ YNDPNRRGLI ENPALLRWAY ARTINVYPNF RPTPKNSLM GALCGFGPLI FIYYIIKTER DRKEKLIQEG KLDRTFHLSY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 21.866955 KDa

Sequence

String:

MAFLASGPYL THQQKVLRLY KRALRHLESW CVQRDKYRYF ACLMRARFEE HKNEKDMAKA TQLLKEAEEE FWYRQHPQPY IFPDSPGGT SYERYDCYKV PEWCLDDWHP SEKAMYPDYF AKREQWKKLR RESWEREVKQ LQEETPPGGP LTEALPPARK E GDLPPLWW YIVTRPRERP M

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #25: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 51.613547 KDa

Sequence

String:

MLKLIVPTIM LLPLTWLSKK HMIWINTTTH SLIISIIPLL FFNQINNNLF SCSPTFSSDP LTTPLLMLTT WLLPLTIMAS QRHLSSEPL SRKKLYLSML ISLQISLIMT FTATELIMFY IFFETTLIPT LAIITRWGNQ PERLNAGTYF LFYTLVGSLP L LIALIYTH NTLGSLNILL LTLTAQELSN SWANNLMWLA YTMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAVLLKL GG YGMMRLT LILNPLTKHM AYPFLVLSLW GMIMTSSICL RQTDLKSLIA YSSISHMALV VTAILIQTPW SFTGAVILMI AHG LTSSLL FCLANSNYER THSRIMILSQ GLQTLLPLMA FWWLLASLAN LALPPTINLL GELSVLVTTF SWSNITLLLT GLNM LVTAL YSLYMFTTTQ WGSLTHHINN MKPSFTRENT LMFMHLSPIL LLSLNPDIIT GFSS

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #26: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.676266 KDa

Sequence

String:

MPMANLLLLI VPILIAMAFL MLTERKILGY MQLRKGPNVV GPYGLLQPFA DAMKLFTKEP LKPATSTITL YITAPTLALT IALLLWTPL PMPNPLVNLN LGLLFILATS SLAVYSILWS GWASNSNYAL IGALRAVAQT ISYEVTLAII LLSTLLMSGS F NLSTLITT QEHLWLLLPS WPLAMMWFIS TLAETNRTPF DLAEGESELV SGFNIEYAAG PFALFFMAEY TNIIMMNTLT TT IFLGTTY DALSPELYTT YFVTKTLLLT SLFLWIRTAY PRFRYDQLMH LLWKNFLPLT LALLMWYVSM PITISSIPPQ T

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.1391 KDa

Sequence

String:

MPGIVELPTL EELKVDEVKI SSAVLKAAAH HYGAQCDKPN KEFMLCRWEE KDPRRCLEEG KLVNKCALDF FRQIKRHCAE PFTEYWTCI DYTGQQLFRH CRKQQAKFDE CVLDKLGWVR PDLGELSKVT KVKTDRPLPE NPYHSRPRPD PSPEIEGDLQ P ATHGSRFY FWTK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 16.435045 KDa

Sequence

String:

MGAHLVRRYL GDASVEPDPL QMPTFPPDYG FPERKEREMV ATQQEMMDAQ LRLQLRDYCA HHLIRLLKCK RDSFPNFLAC KQERHDWDY CEHRDYVMRM KEFERERRLL QRKKRREKKA AELAKGQGPG EVDPKVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #29: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mi...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 40.810594 KDa

Sequence

String:

MALRLLKLAA TSASARVVAA GAQRVRGIHS SVQCKLRYGM WHFLLGDKAS KRLTERSRVI TVDGNICTGK GKLAKEIAEK LGFKHFPEA GIHYPDSTTG DGKPLATDYN GNCSLEKFYD DPRSNDGNSY RLQSWLYSSR LLQYSDALEH LLTTGQGVVL E RSIFSDFV FLEAMYNQGF IRKQCVDHYN EVKSVTICDY LPPHLVIYID VPVPEVQRRI QKKGDPHEMK ITSAYLQDIE NA YKKTFLP EMSEKCEVLQ YSAREAQDSK KVVEDIEYLK FDKGPWLKQD NRTLYHLRLL VQDKFEVLNY TSIPIFLPEV TIG AHQTDR VLHQFRELPG RKYSPGYNTE VGDKWIWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Macromolecule #30: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 50.886031 KDa

Sequence

String:

MLATRRLLGW SLPARVSVRF SGDTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGSLSRG DWYKTKEILL KGPDWILGEI KTSGLRGRG GAGFPTGLKW SFMNKPSDGR PKYLVVNADE GEPGTCKDRE ILRHDPHKLL EGCLVGGRAM GARAAYIYIR G EFYNEASN LQVAIREAYE AGLIGKNACG SGYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PT TVANVET VAVSPTICRR GGTWFAGFGR ERNSGTKLFN ISGHVNHPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPG GSSTPL IPKSVCETVL MDFDALVQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFV RGDAR PAEIDSLWEI SKQIEGHTIC ALGDGAAWPV QGLIRHFRPE LEERMQRFAQ QHQARQAAS

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Macromolecule #31: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.734117 KDa

Sequence

String:

MRCLTTPMLL RALAQAARAG PPGGRSLHSS AVAATYKYVN MQDPEMDMKS VTDRAARTLL WTELFRGLGM TLSYLFREPA TINYPFEKG PLSPRFRGEH ALRRYPSGEE RCIACKLCEA ICPAQAITIE AEPRADGSRR TTRYDIDMTK CIYCGFCQEA C PVDAIVEG PNFEFSTETH EELLYNKEKL LNNGDKWEAE IAANIQADYL YR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Macromolecule #32: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.675533 KDa

Sequence

String:

MAVLSAPGLR GFRILGLRSS VGPAVQARGV HQSVATDGPS STQPALPKAR AVAPKPS(SEP)RG EYVVAKLDDL VNWARR SSL WPMTFGLACC AVEMMHMAAP RYDMDRFGVV FRASPRQSDV MIVAGTLTNK MAPALRKVYD QMPEPRYVVS MGSCANG GG YYHYSYSVVR GCDRIVPVDI YIPGCPPTAE ALLYGILQLQ RKIKRERRLQ IWYRR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #33: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.165603 KDa

Sequence

String:

MAGSGVRQAT STASTFVKPI FSRDMNEAKR RVRELYRAWY REVPNTVHQF QLDITVKMGR DKVREMFMKN AHVTDPRVVD LLVIKGKIE LEETIKVWKQ RTHVMRFFHE TEAPRPKDFL SKFYVGHDP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #34: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.937563 KDa

Sequence

String:

MAAAAASRGV GAKLGLREIR IHLCQRSPGS QGVRDFIEKR YVELKKANPD LPILIRECSD VQPKLWARYA FGQETNVPLN NFSADQVTR ALENVLSGKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.477665 KDa

Sequence

String:

MAGVLKKTTG LVGLAVCNTP HERLRILYTK ILDVLEEIPK NAAYRKYTEQ ITNEKLAMVK AEPDVKKLED QLQGGQLEEV ILQAEHELN LARKMREWKL WEPLVEEPPA DQWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 42.575527 KDa

Sequence

String:

MAAAAQSRVV RVLSMSRSAI TAIATSVCHG PPCRQLHHAL MPHGKGGRSS VSGIVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDKY DIMHLRPMGD LGQLLFLEWD ARDKDSIRRV VQHSNVVINL IGRDWETKNF DFEDVFVKIP QAIAQLSKEA G VEKFIHVS HLNANIKSSS RYLRNKAVGE KVVRDAFPEA IIVKPSDIFG REDRFLNSFA SMHRFGPIPL GSLGWKTVKQ PV YVVDVSK GIVNAVKDPD ANGKSFAFVG PSRYLLFHLV KYIFAVAHRL FLPFPLPLFA YRWVARVFEI SPFEPWITRD KVE RMHITD MKLPHLPGLE DLGIQATPLE LKAIEVLRRH RTYRWLSAEI EDVKPAKTVN I

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Macromolecule #37: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.958526 KDa

Sequence

String:

MAAPCLLRQG RAGALKTMLQ EAQVFRGLAS TVSLSAESGK SEKGQPQNSK KQSPPKKPAP VPAEPFDNTT YKNLQHHDYS TYTFLDLNL ELSKFRMPQP SSGRESPRH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial

Macromolecule #38: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 20.139 KDa

Sequence

String:

MAAVSMSVVL RQTLWRRRAV AVAALSVSRV PTRSLRTSTW RLAQDQTQDT QLITVDEKLD ITTLTGVPEE HIKTRKVRIF VPARNNMQS GVNNTKKWKM EFDTRERWEN PLMGWASTAD PLSNMVLTFS TKEDAVSFAE KNGWSYDIEE RKVPKPKSKS Y GANFSWNK RTRVSTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

Macromolecule #39: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

• Macromolecule: Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 79.557383 KDa

Sequence

String:

MLRIPVRKAL VGLSKSPKGC VRTTATAASN LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVV AACAMPVMKG WNILTNSEKS KKAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGND RSRFLEGKRA V EDKNIGPL VKTIMTRCIQ CTRCIRFASE IAGVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNIIDICPV GALTSKPYAF TA RPWETRK TESIDVMDAV GSNIVVSTRT GEVMRILPRM HEDINEEWIS DKTRFAYDGL KRQRLTEPMV RNEKGLLTYT SWE DALSRV AGMLQSFQGK DVAAIAGGLV DAEALVALKD LLNRVDSDTL CTEEVFPTAG AGTDLRSNYL LNTTIAGVEE ADVV LLVGT NPRFEAPLFN ARIRKSWLHN DLKVALIGSP VDLTYTYDHL GDSPKILQDI ASGSHPFSQV LKEAKKPMVV LGSSA LQRN DGAAILAAVS SIAQKIRMTS GVTGDWKVMN ILHRIASQVA ALDLGYKPGV EAIRKNPPKV LFLLGADGGC ITRQDL PKD CFIIYQGHHG DVGAPIADVI LPGAAYTEKS ATYVNTEGRA QQTKVAVTPP GLAREDWKII RALSEIAGMT LPYDTLD QV RNRLEEVSPN LVRYDDIEGA NYFQQANELS KLVNQQLLAD PLVPPQLTIK DFYMTDSISR ASQTMAKCVK AVTEGAQA V EEPSIC

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.140445 KDa

Sequence

String:

MELVQVLKRG LQQITGHGGL RGYLRVFFRT NDAKVGTLVG EDKYGNKYYE DNKQFFGRHR WVVYTTEMNG KNTFWDVDGS MVPPEWHRW LHSMTDDPPT TKPLTARKFI WTNHKFNVTG TPEQYVPYST TRKKIQEWIP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Macromolecule #41: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.425555 KDa

Sequence

String:

MFFSAALRAR AAGLTAHWGR HVRNLHKTVM QNGAGGALFV HRDTPENNPD TPFDFTPENY KRIEAIVKNY PEGHKAAAVL PVLDLAQRQ NGWLPISAMN KVAEVLQVPP MRVYEVATFY TMYNRKPVGK YHIQVCTTTP CMLRNSDSIL EAIQKKLGIK V GETTPDKL FTLIEVECLG ACVNAPMVQI NDNYYEDLTA KDIEEIIDEL KAGKIPKPGP RSGRFSCEPA GGLTSLTEPP KG PGFGVQA GL

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Macromolecule #42: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 30.28149 KDa

Sequence

String:

MAAAAVARLW WRGILGASAL TRGTGRPSVL LLPVRRESAG ADTRPTVRPR NDVAHKQLSA FGEYVAEILP KYVQQVQVSC FNELEVCIH PDGVIPVLTF LRDHTNAQFK SLVDLTAVDV PTRQNRFEIV YNLLSLRFNS RIRVKTYTDE LTPIESAVSV F KAANWYER EIWDMFGVFF ANHPDLRRIL TDYGFEGHPF RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP WE AFPVYRQ PPESLKLEAG DKKPDAK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Macromolecule #43: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.733654 KDa

Sequence

String:

MAAAMTFCRL LNRCGEAARS LPLGARCFGV RVSPTGEKVT HTGQVYDDKD YRRIRFVGRQ KEVNENFAID LIAEQPVSEV ETRVIACDG GGGALGHPKV YINLDKETKT GTCGYCGLQF RQHHH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #44: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.571403 KDa

Sequence

String:

MASATRLIQR LRNWASGHDL QGKLQLRYQE ISKRTQPPPK LPVGPSHKLS NNYYCTRDGR RESVPPSIIM SSQKALVSGK PAESSAVAA TEKKAVTPAP PIKRWELSSD QPYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Macromolecule #45: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

• Macromolecule: Name: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / type: ligand / ID: 45 / Number of copies: 7 / Formula: PEE
• Molecular weight: Theoretical: 744.034 Da

Chemical component information

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

Macromolecule #46: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 46 / Number of copies: 6 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Macromolecule #47: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

• Macromolecule: Name: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate; type: ligand / ID: 47 / Number of copies: 2 / Formula: 8Q1
• Molecular weight: Theoretical: 540.651 Da

Chemical component information

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

Macromolecule #48: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,...

• Macromolecule: Name: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL; type: ligand / ID: 48 / Number of copies: 7 / Formula: PLX
• Molecular weight: Theoretical: 767.132 Da

Chemical component information

ChemComp-PLX:
(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / phospholipid*YM

Macromolecule #49: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 49 / Number of copies: 1 / Formula: DGT
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-DGT:
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE

Macromolecule #50: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 50 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #51: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 51 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #52: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 52 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #53: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 53 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #54: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 54 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #55: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 55 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #56: water

• Macromolecule: Name: water / type: ligand / ID: 56 / Number of copies: 1 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average exposure time: 3.5 sec. / Average electron dose: 35.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.4 µm
• Sample stage: Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

5xtd

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.63 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 168805
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: NOT APPLICABLE