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- EMDB-5239: human Low Density Lipoprotein -

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Basic information

Entry
Database: EMDB / ID: EMD-5239
Titlehuman Low Density Lipoprotein
Map dataIso-surface rendering of the overall structure of the human LDL particle
Sample
  • Sample: human Low Density Lipoprotein
  • Organelle or cellular component: humang Low Density Lipoprotein
KeywordsLDL / cholesterol ester / apoB / atherosclerosis
Biological speciesunidentified (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 26.0 Å
AuthorsLiu Y / Atkinson D
CitationJournal: J Mol Biol / Year: 2011
Title: Enhancing the contrast of ApoB to locate the surface components in the 3D density map of human LDL.
Authors: Yuhang Liu / David Atkinson /
Abstract: A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal- ...A 26 Å resolution map of the structure of human low-density lipoprotein (LDL) was obtained from electron cryomicroscopy and single-particle image reconstruction. The structure showed a discoidal-shaped LDL particle with high-density regions mainly distributed at the edge of the particle and low-density regions at the flat surface that covers the core region. To determine the chemical components that correspond to these density regions and to delineate the distribution of protein and phospholipid located at the particle surface at the resolution of the map, we used Mono-Sulfo-NHS-Undecagold labeling to increase preferentially the contrast of the apolipoprotein B component on the LDL particle. In the three-dimensional map from the image reconstruction of the undecagold-labeled LDL particles, the high-density region from the undecagold label was distributed mainly at the edge of the particle, and lower density regions were found at the flat surfaces that cover the neutral lipid core. This suggests that apolipoprotein B mainly encircles LDL at the edge of the particle and the phospholipid monolayers are located at the flat surfaces, which are parallel to the cholesterol ester layers in the core and may interact with the core lipid layers through the acyl chains.
History
DepositionOct 14, 2010-
Header (metadata) releaseJan 3, 2011-
Map releaseJan 3, 2011-
UpdateDec 3, 2014-
Current statusDec 3, 2014Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 1.2
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 1.2
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_5239_1.jpg

Downloads & links

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Map

FileDownload / File: emd_5239.map.gz / Format: CCP4 / Size: 1001 KB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationIso-surface rendering of the overall structure of the human LDL particle
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
5.8 Å/pix.
x 64 pix.
= 371.2 Å		5.8 Å/pix.
x 64 pix.
= 371.2 Å		5.8 Å/pix.
x 64 pix.
= 371.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 5.8 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.2 / Movie #1: 1.2
Minimum - Maximum-0.00030867 - 2.94348454
Average (Standard dev.)0.99999994 (±0.29761904)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-32-32-32
Dimensions646464
Spacing646464
CellA=B=C: 371.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z5.85.85.8
M x/y/z646464
origin x/y/z0.0000.0000.000
length x/y/z371.200371.200371.200
α/β/γ90.00090.00090.000
start NX/NY/NZ-62-62-62
NX/NY/NZ125125125
MAP C/R/S123
start NC/NR/NS-32-32-32
NC/NR/NS646464
D min/max/mean-0.0002.9431.000

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Supplemental data

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Sample components

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Entire : human Low Density Lipoprotein

EntireName: human Low Density Lipoprotein
Components
  • Sample: human Low Density Lipoprotein
  • Organelle or cellular component: humang Low Density Lipoprotein

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Supramolecule #1000: human Low Density Lipoprotein

SupramoleculeName: human Low Density Lipoprotein / type: sample / ID: 1000 / Oligomeric state: monomer / Number unique components: 1
Molecular weightTheoretical: 2.2 MDa

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Supramolecule #1: humang Low Density Lipoprotein

SupramoleculeName: humang Low Density Lipoprotein / type: organelle_or_cellular_component / ID: 1 / Name.synonym: LDL / Number of copies: 1 / Oligomeric state: monomer / Recombinant expression: No / Database: NCBI
Source (natural)Organism: unidentified (others) / synonym: human / Tissue: plasma
Molecular weightTheoretical: 2.2 MDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.4 / Details: 10mM phosphate, 150mM sodium chloride
GridDetails: 400 mesh Cu grid
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 89 K / Instrument: OTHER / Details: Vitrification instrument: vitrobot / Method: Blot for 2 seconds before plunging

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Electron microscopy

MicroscopeFEI TECNAI 20
TemperatureAverage: 90 K
Alignment procedureLegacy - Astigmatism: objective lens astigmatism was corrected at 200,000 times
DateMar 14, 2010
Image recordingCategory: CCD / Film or detector model: GENERIC CCD / Digitization - Sampling interval: 2.9 µm / Average electron dose: 25 e/Å2
Electron beamAcceleration voltage: 120 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 29000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2 mm / Nominal defocus max: 4.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 29000
Sample stageSpecimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN

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Image processing

CTF correctionDetails: ctfit
Final reconstructionAlgorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 26.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: EMAN / Number images used: 31000

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