+
データを開く
-
基本情報
登録情報 | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
タイトル | Mouse mitoribosome large subunit assembly intermediate (without uL16m) bound to MRM3-dimer, DDX28 and the MALSU-L0R8F8-mt-ACP complex, State A1 (SAMC knock-out) | ||||||||||||
![]() | |||||||||||||
![]() |
| ||||||||||||
![]() | mitochondria / ribosome / large subunit / assembly intermediate | ||||||||||||
機能・相同性 | ![]() Mitochondrial translation elongation / Mitochondrial translation termination / rRNA (guanosine-2'-O-)-methyltransferase activity / ribonucleoprotein granule / negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / positive regulation of mitochondrial translation ...Mitochondrial translation elongation / Mitochondrial translation termination / rRNA (guanosine-2'-O-)-methyltransferase activity / ribonucleoprotein granule / negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / positive regulation of mitochondrial translation / rRNA import into mitochondrion / Respiratory electron transport / mitochondrial translational termination / : / mitochondrial translational elongation / ribonuclease III activity / translation release factor activity, codon nonspecific / Mitochondrial protein degradation / mitochondrial [2Fe-2S] assembly complex / mitochondrial large ribosomal subunit / iron-sulfur cluster assembly complex / mitochondrial fission / mitochondrial large ribosomal subunit binding / peptidyl-tRNA hydrolase / mitochondrial ribosome / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エンドリボヌクレアーゼ / mitochondrial small ribosomal subunit / peptidyl-tRNA hydrolase activity / mitochondrial translation / [2Fe-2S] cluster assembly / iron-sulfur cluster assembly / mitochondrial respiratory chain complex I assembly / mitochondrial nucleoid / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / RNA processing / cytosolic ribosome / rescue of stalled ribosome / aerobic respiration / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / cellular response to leukemia inhibitory factor / ribosomal large subunit biogenesis / fatty acid biosynthetic process / cell junction / double-stranded RNA binding / 5S rRNA binding / RNA helicase activity / mitochondrial inner membrane / rRNA binding / nuclear body / RNA helicase / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / ribonucleoprotein complex / protein domain specific binding / nucleotide binding / apoptotic process / nucleolus / structural molecule activity / ATP hydrolysis activity / mitochondrion / RNA binding / nucleoplasm / ATP binding / metal ion binding / identical protein binding / nucleus / plasma membrane / cytosol 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.67 Å | ||||||||||||
![]() | Singh V / Glasgow RIC / Rorbach J / Freyer C / Amunts A / Wredenberg A | ||||||||||||
資金援助 | ![]() ![]()
| ||||||||||||
![]() | ![]() タイトル: The mitochondrial methylation potential gates mitoribosome assembly. 著者: Ruth I C Glasgow / Vivek Singh / Lucía Peña-Pérez / Alissa Wilhalm / Marco F Moedas / David Moore / Florian A Rosenberger / Xinping Li / Ilian Atanassov / Mira Saba / Miriam Cipullo / ...著者: Ruth I C Glasgow / Vivek Singh / Lucía Peña-Pérez / Alissa Wilhalm / Marco F Moedas / David Moore / Florian A Rosenberger / Xinping Li / Ilian Atanassov / Mira Saba / Miriam Cipullo / Joanna Rorbach / Anna Wedell / Christoph Freyer / Alexey Amunts / Anna Wredenberg / ![]() ![]() 要旨: S-adenosylmethionine (SAM) is the principal methyl donor in cells and is essential for mitochondrial gene expression, influencing RNA modifications, translation, and ribosome biogenesis. Using direct ...S-adenosylmethionine (SAM) is the principal methyl donor in cells and is essential for mitochondrial gene expression, influencing RNA modifications, translation, and ribosome biogenesis. Using direct long-read RNA sequencing in mouse tissues and embryonic fibroblasts, we show that processing of the mitochondrial ribosomal gene cluster fails in the absence of mitochondrial SAM, leading to an accumulation of unprocessed precursors. Proteomic analysis of ribosome fractions revealed these precursors associated with processing and assembly factors, indicating stalled biogenesis. Structural analysis by cryo-electron microscopy demonstrated that SAM-dependent methylation is required for peptidyl transferase centre formation during mitoribosome assembly. Our findings identify a critical role for SAM in coordinating mitoribosomal RNA processing and large subunit maturation, linking cellular methylation potential to mitochondrial translation capacity. | ||||||||||||
履歴 |
|
-
構造の表示
添付画像 |
---|
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 287.5 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 72.3 KB 72.3 KB | 表示 表示 | ![]() |
画像 | ![]() | 49.7 KB | ||
Filedesc metadata | ![]() | 16 KB | ||
その他 | ![]() ![]() | 425.6 MB 425.6 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1.1 MB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 1.1 MB | 表示 | |
XML形式データ | ![]() | 19.8 KB | 表示 | |
CIF形式データ | ![]() | 23.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 9hccMC ![]() 9e9cC ![]() 9hcdC ![]() 9hceC ![]() 9hcfC ![]() 9hcgC ![]() 9hchC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.84684 Å | ||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
|
-添付データ
-ハーフマップ: #2
ファイル | emd_52044_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_52044_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-
試料の構成要素
+全体 : Assembly intermediate of the mitoribosome large subunit
+超分子 #1: Assembly intermediate of the mitoribosome large subunit
+分子 #1: 16S rRNA (1584-MER)
+分子 #2: tRNA-Phe (68-MER)
+分子 #3: Large ribosomal subunit protein uL2m
+分子 #4: Large ribosomal subunit protein uL3m
+分子 #5: Large ribosomal subunit protein uL4m
+分子 #6: Large ribosomal subunit protein bL9m
+分子 #7: Large ribosomal subunit protein uL10m
+分子 #8: Large ribosomal subunit protein uL11m
+分子 #9: Large ribosomal subunit protein uL13m
+分子 #10: Large ribosomal subunit protein uL14m
+分子 #11: Large ribosomal subunit protein uL15m
+分子 #12: Large ribosomal subunit protein bL17m
+分子 #13: Large ribosomal subunit protein uL18m
+分子 #14: Large ribosomal subunit protein bL19m
+分子 #15: Large ribosomal subunit protein bL20m
+分子 #16: Large ribosomal subunit protein bL21m
+分子 #17: Large ribosomal subunit protein uL22m
+分子 #18: Large ribosomal subunit protein uL23m
+分子 #19: Large ribosomal subunit protein uL24m
+分子 #20: Large ribosomal subunit protein bL28m,Large ribosomal subunit pro...
+分子 #21: Large ribosomal subunit protein uL29m
+分子 #22: Large ribosomal subunit protein uL30m
+分子 #23: Large ribosomal subunit protein bL32m
+分子 #24: Large ribosomal subunit protein mL37
+分子 #25: Large ribosomal subunit protein mL38
+分子 #26: Large ribosomal subunit protein mL39
+分子 #27: Large ribosomal subunit protein mL40
+分子 #28: Large ribosomal subunit protein mL41
+分子 #29: Large ribosomal subunit protein mL42
+分子 #30: Large ribosomal subunit protein mL43
+分子 #31: Large ribosomal subunit protein mL44
+分子 #32: Large ribosomal subunit protein mL45
+分子 #33: Large ribosomal subunit protein mL46
+分子 #34: Large ribosomal subunit protein mL48
+分子 #35: Large ribosomal subunit protein mL49
+分子 #36: Large ribosomal subunit protein mL50
+分子 #37: Large ribosomal subunit protein mL51
+分子 #38: Large ribosomal subunit protein mL52
+分子 #39: Large ribosomal subunit protein mL53
+分子 #40: Large ribosomal subunit protein mL54
+分子 #41: Large ribosomal subunit protein mL63
+分子 #42: Large ribosomal subunit protein mL62
+分子 #43: Large ribosomal subunit protein mL64
+分子 #44: Large ribosomal subunit protein mL66
+分子 #45: Large ribosomal subunit protein mL65
+分子 #46: Mitochondrial assembly of ribosomal large subunit protein 1
+分子 #47: Predicted gene, 55359
+分子 #48: Acyl carrier protein, mitochondrial
+分子 #49: rRNA methyltransferase 3, mitochondrial
+分子 #50: Probable ATP-dependent RNA helicase DDX28
+分子 #51: ZINC ION
+分子 #52: FE2/S2 (INORGANIC) CLUSTER
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 7.5 |
---|---|
凍結 | 凍結剤: ETHANE |
-
電子顕微鏡法
顕微鏡 | TFS KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.4 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |