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- EMDB-52047: Mouse mitoribosome large subunit assembly intermediate bound to N... -
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Open data
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Basic information
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Title | Mouse mitoribosome large subunit assembly intermediate bound to NSUN4, METRF4, GTPBP7, GTPBP10 and the MALSU-L0R8F8-mtACP complex with uL16m, State B2 (SAMC knock-out) | ||||||||||||
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![]() | mitochondria / ribosome assembly / large subunit / RIBOSOME | ||||||||||||
Function / homology | ![]() Mitochondrial translation elongation / Mitochondrial translation termination / mRNA (cytidine-5-)-methyltransferase activity / regulation of respiratory system process / mitochondrial RNA catabolic process / regulation of mitochondrial translation / negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation ...Mitochondrial translation elongation / Mitochondrial translation termination / mRNA (cytidine-5-)-methyltransferase activity / regulation of respiratory system process / mitochondrial RNA catabolic process / regulation of mitochondrial translation / negative regulation of mitochondrial translation / mitochondrial large ribosomal subunit assembly / Protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Complex I biogenesis / positive regulation of mitochondrial translation / rRNA import into mitochondrion / Respiratory electron transport / mitochondrial transcription / mitochondrial translational termination / : / mitochondrial translational elongation / ribonuclease III activity / translation release factor activity, codon nonspecific / Mitochondrial protein degradation / camera-type eye development / mitochondrial [2Fe-2S] assembly complex / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit / mitochondrial fission / mitochondrial large ribosomal subunit binding / peptidyl-tRNA hydrolase / rRNA methylation / mitochondrial ribosome / Hydrolases; Acting on ester bonds; Endoribonucleases producing 5'-phosphomonoesters / mitochondrial small ribosomal subunit / peptidyl-tRNA hydrolase activity / [2Fe-2S] cluster assembly / mitochondrial translation / protein targeting to mitochondrion / iron-sulfur cluster assembly / mitochondrial respiratory chain complex I assembly / proton motive force-driven mitochondrial ATP synthesis / respiratory chain complex I / RNA processing / rescue of stalled ribosome / cellular response to leukemia inhibitory factor / aerobic respiration / Transferases; Transferring one-carbon groups; Methyltransferases / ribosome assembly / ribosomal large subunit biogenesis / cytosolic ribosome assembly / rRNA processing / fatty acid biosynthetic process / cell junction / ribosome biogenesis / double-stranded RNA binding / heart development / 5S rRNA binding / double-stranded DNA binding / small ribosomal subunit rRNA binding / mitochondrial inner membrane / rRNA binding / nuclear body / structural constituent of ribosome / mitochondrial matrix / protein domain specific binding / translation / ribonucleoprotein complex / nucleotide binding / GTPase activity / apoptotic process / regulation of DNA-templated transcription / GTP binding / nucleolus / magnesium ion binding / mitochondrion / RNA binding / nucleoplasm / metal ion binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.85 Å | ||||||||||||
![]() | Singh V / Rorbach J / Freyer C / Amunts A / Wredenberg A | ||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: The mitochondrial methylation potential gates mitoribosome assembly. Authors: Ruth I C Glasgow / Vivek Singh / Lucía Peña-Pérez / Alissa Wilhalm / Marco F Moedas / David Moore / Florian A Rosenberger / Xinping Li / Ilian Atanassov / Mira Saba / Miriam Cipullo / ...Authors: Ruth I C Glasgow / Vivek Singh / Lucía Peña-Pérez / Alissa Wilhalm / Marco F Moedas / David Moore / Florian A Rosenberger / Xinping Li / Ilian Atanassov / Mira Saba / Miriam Cipullo / Joanna Rorbach / Anna Wedell / Christoph Freyer / Alexey Amunts / Anna Wredenberg / ![]() ![]() Abstract: S-adenosylmethionine (SAM) is the principal methyl donor in cells and is essential for mitochondrial gene expression, influencing RNA modifications, translation, and ribosome biogenesis. Using direct ...S-adenosylmethionine (SAM) is the principal methyl donor in cells and is essential for mitochondrial gene expression, influencing RNA modifications, translation, and ribosome biogenesis. Using direct long-read RNA sequencing in mouse tissues and embryonic fibroblasts, we show that processing of the mitochondrial ribosomal gene cluster fails in the absence of mitochondrial SAM, leading to an accumulation of unprocessed precursors. Proteomic analysis of ribosome fractions revealed these precursors associated with processing and assembly factors, indicating stalled biogenesis. Structural analysis by cryo-electron microscopy demonstrated that SAM-dependent methylation is required for peptidyl transferase centre formation during mitoribosome assembly. Our findings identify a critical role for SAM in coordinating mitoribosomal RNA processing and large subunit maturation, linking cellular methylation potential to mitochondrial translation capacity. | ||||||||||||
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 487.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 81.4 KB 81.4 KB | Display Display | ![]() |
Images | ![]() | 68.9 KB | ||
Filedesc metadata | ![]() | 17.2 KB | ||
Others | ![]() ![]() ![]() | 336.9 MB 488.5 MB 488.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 19.5 KB | Display | |
Data in CIF | ![]() | 23.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9hcfMC ![]() 9hccC ![]() 9hcdC ![]() 9hceC ![]() 9hcgC ![]() 9hchC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | unsharpened map | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.84684 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: local resolution filtered map
File | emd_52047_additional_1.map | ||||||||||||
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Annotation | local resolution filtered map | ||||||||||||
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Density Histograms |
-Half map: half map 1
File | emd_52047_half_map_1.map | ||||||||||||
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Annotation | half map 1 | ||||||||||||
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Density Histograms |
-Half map: half map 2
File | emd_52047_half_map_2.map | ||||||||||||
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Annotation | half map 2 | ||||||||||||
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Sample components
+Entire : mitoribosome large subunit assembly intermediate
+Supramolecule #1: mitoribosome large subunit assembly intermediate
+Macromolecule #1: 16S rRNA (1432-MER)
+Macromolecule #2: RNA (63-MER)
+Macromolecule #3: Large ribosomal subunit protein uL2m
+Macromolecule #4: Large ribosomal subunit protein uL3m
+Macromolecule #5: Large ribosomal subunit protein uL4m
+Macromolecule #6: Large ribosomal subunit protein bL9m
+Macromolecule #7: Large ribosomal subunit protein uL10m
+Macromolecule #8: Large ribosomal subunit protein uL11m
+Macromolecule #9: Large ribosomal subunit protein uL13m
+Macromolecule #10: Large ribosomal subunit protein uL14m
+Macromolecule #11: Large ribosomal subunit protein uL15m
+Macromolecule #12: Large ribosomal subunit protein uL16m
+Macromolecule #13: Large ribosomal subunit protein bL17m
+Macromolecule #14: Large ribosomal subunit protein uL18m
+Macromolecule #15: Large ribosomal subunit protein bL19m
+Macromolecule #16: Large ribosomal subunit protein bL20m
+Macromolecule #17: Large ribosomal subunit protein bL21m
+Macromolecule #18: Large ribosomal subunit protein uL22m
+Macromolecule #19: Large ribosomal subunit protein uL23m
+Macromolecule #20: Large ribosomal subunit protein uL24m
+Macromolecule #21: Large ribosomal subunit protein bL27m
+Macromolecule #22: Large ribosomal subunit protein bL28m,Large ribosomal subunit pro...
+Macromolecule #23: Large ribosomal subunit protein uL29m
+Macromolecule #24: Large ribosomal subunit protein uL30m
+Macromolecule #25: 5-cytosine rRNA methyltransferase NSUN4
+Macromolecule #26: Large ribosomal subunit protein bL33m
+Macromolecule #27: Large ribosomal subunit protein bL34m
+Macromolecule #28: Large ribosomal subunit protein bL35m
+Macromolecule #29: Large ribosomal subunit protein bL36m
+Macromolecule #30: Large ribosomal subunit protein mL38
+Macromolecule #31: Large ribosomal subunit protein mL39
+Macromolecule #32: Large ribosomal subunit protein mL43
+Macromolecule #33: Large ribosomal subunit protein mL48
+Macromolecule #34: Large ribosomal subunit protein mL50
+Macromolecule #35: Large ribosomal subunit protein mL51
+Macromolecule #36: Large ribosomal subunit protein mL52
+Macromolecule #37: Large ribosomal subunit protein mL63
+Macromolecule #38: Large ribosomal subunit protein mL64
+Macromolecule #39: Large ribosomal subunit protein mL66
+Macromolecule #40: Large ribosomal subunit protein mL65
+Macromolecule #41: Mitochondrial assembly of ribosomal large subunit protein 1
+Macromolecule #42: Predicted gene, 55359
+Macromolecule #43: Large ribosomal subunit protein mL37
+Macromolecule #44: Large ribosomal subunit protein mL40
+Macromolecule #45: Large ribosomal subunit protein mL41
+Macromolecule #46: Large ribosomal subunit protein mL42
+Macromolecule #47: Large ribosomal subunit protein mL55
+Macromolecule #48: Mitochondrial ribosome-associated GTPase 1
+Macromolecule #49: Large ribosomal subunit protein mL54
+Macromolecule #50: Transcription termination factor 4, mitochondrial
+Macromolecule #51: Acyl carrier protein, mitochondrial
+Macromolecule #52: Large ribosomal subunit protein mL44
+Macromolecule #53: Large ribosomal subunit protein mL49
+Macromolecule #54: Large ribosomal subunit protein mL53
+Macromolecule #55: Large ribosomal subunit protein bL32m
+Macromolecule #56: Large ribosomal subunit protein mL45
+Macromolecule #57: Large ribosomal subunit protein mL46
+Macromolecule #58: Large ribosomal subunit protein mL62
+Macromolecule #59: GTP-binding protein 10
+Macromolecule #60: MAGNESIUM ION
+Macromolecule #61: FE2/S2 (INORGANIC) CLUSTER
+Macromolecule #62: ZINC ION
+Macromolecule #63: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.4 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |