ジャーナル: Virology / 年: 2011 タイトル: The structure of avian polyomavirus reveals variably sized capsids, non-conserved inter-capsomere interactions, and a possible location of the minor capsid protein VP4. 著者: Peter S Shen / Dirk Enderlein / Christian D S Nelson / Weston S Carter / Masaaki Kawano / Li Xing / Robert D Swenson / Norman H Olson / Timothy S Baker / R Holland Cheng / Walter J Atwood / ...著者: Peter S Shen / Dirk Enderlein / Christian D S Nelson / Weston S Carter / Masaaki Kawano / Li Xing / Robert D Swenson / Norman H Olson / Timothy S Baker / R Holland Cheng / Walter J Atwood / Reimar Johne / David M Belnap / 要旨: Avian polyomavirus (APV) causes a fatal, multi-organ disease among several bird species. Using cryogenic electron microscopy and other biochemical techniques, we investigated the structure of APV and ...Avian polyomavirus (APV) causes a fatal, multi-organ disease among several bird species. Using cryogenic electron microscopy and other biochemical techniques, we investigated the structure of APV and compared it to that of mammalian polyomaviruses, particularly JC polyomavirus and simian virus 40. The structure of the pentameric major capsid protein (VP1) is mostly conserved; however, APV VP1 has a unique, truncated C-terminus that eliminates an intercapsomere-connecting β-hairpin observed in other polyomaviruses. We postulate that the terminal β-hairpin locks other polyomavirus capsids in a stable conformation and that absence of the hairpin leads to the observed capsid size variation in APV. Plug-like density features were observed at the base of the VP1 pentamers, consistent with the known location of minor capsid proteins VP2 and VP3. However, the plug density is more prominent in APV and may include VP4, a minor capsid protein unique to bird polyomaviruses.
名称: Simian virus 40 / タイプ: virus / ID: 1 / Name.synonym: SV40 / 詳細: Cryo-EM of simian virus 40 over holey carbon grids / NCBI-ID: 10633 / 生物種: Simian virus 40 / データベース: NCBI / ウイルスタイプ: VIRION / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: No / ウイルス・中空状態: No / Syn species name: SV40
宿主
別称: VERTEBRATES
ウイルス殻
Shell ID: 1 / 名称: VP1 / 直径: 500 Å / T番号(三角分割数): 7
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
緩衝液
pH: 7.4
グリッド
詳細: 400 mesh copper grid (holey carbon)
凍結
凍結剤: ETHANE / 装置: HOMEMADE PLUNGER 詳細: Vitrification instrument: homemade. vitrification carried out in normal, room atmosphere 手法: blotted for a few seconds before plunging
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電子顕微鏡法
顕微鏡
FEI/PHILIPS EM420
撮影
カテゴリ: FILM / フィルム・検出器のモデル: KODAK SO-163 FILM デジタル化 - スキャナー: NIKON SUPER COOLSCAN 9000 デジタル化 - サンプリング間隔: 1.75 µm / 実像数: 10 / 平均電子線量: 11.5 e/Å2 / 詳細: Micrographs digitized in positive contrast mode. / ビット/ピクセル: 16