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- EMDB-4874: Cryo-EM structure of a respiratory complex I mutant lacking NDUFS4 -

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Basic information

Entry
Database: EMDB / ID: EMD-4874
TitleCryo-EM structure of a respiratory complex I mutant lacking NDUFS4
Map data
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: x 41 types
  • Ligand: x 6 types
KeywordsComplex I / NADH dehydrogenase / Mitochondrion Proton pumping / Ubiquinone / OXIDOREDUCTASE
Function / homology
Function and homology information


lipoate biosynthetic process / NADH dehydrogenase / : / TIM23 mitochondrial import inner membrane translocase complex / protein import into mitochondrial matrix / NADH dehydrogenase complex / ubiquinone biosynthetic process / mitochondrial [2Fe-2S] assembly complex / oxidoreductase activity, acting on NAD(P)H / iron-sulfur cluster assembly ...lipoate biosynthetic process / NADH dehydrogenase / : / TIM23 mitochondrial import inner membrane translocase complex / protein import into mitochondrial matrix / NADH dehydrogenase complex / ubiquinone biosynthetic process / mitochondrial [2Fe-2S] assembly complex / oxidoreductase activity, acting on NAD(P)H / iron-sulfur cluster assembly / acyl binding / ubiquinone binding / acyl carrier activity / electron transport coupled proton transport / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / aerobic respiration / respiratory electron transport chain / electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / oxidoreductase activity / protein-containing complex binding / mitochondrion / metal ion binding / membrane
Similarity search - Function
NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 ...NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / SLBB domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / NADH dehydrogenase, subunit C / Complex 1 LYR protein domain / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / Complex 1 protein (LYR family) / NADH:ubiquinone oxidoreductase / : / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / NADH:quinone oxidoreductase/Mrp antiporter, TM / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / 2Fe-2S iron-sulfur cluster binding domain / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / NAD-dependent epimerase/dehydratase / NAD dependent epimerase/dehydratase family / NuoE domain / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / CHCH
Similarity search - Domain/homology
Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein ...Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / Thioredoxin-like protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / Uncharacterized protein / Zinc finger CHCC-type domain-containing protein / NAD-dependent epimerase/dehydratase domain-containing protein / NADH dehydrogenase 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase / ETC complex I subunit conserved region-domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / YALI0D10274p / YALI0E23749p / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / YALI0A17946p / YALI0F06061p / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / YALI0F00924p / YALI0F17248p / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / YALI0E29095p / YALI0D00737p / YALI0F02123p / YALI0D05467p / YALI0F14003p / YALI0F06050p / YALI0E28424p / YALI0E23089p / YALI0E11891p / Acyl carrier protein / YALI0D24585p / YALI0D19030p / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / YALI0D04939p / YALI0C03201p / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit N7BM / NADH-ubiquinone oxidoreductase / YALI0A02651p / YALI0A01419p / NUWM protein / NUVM protein / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 6 / Subunit NUKM of protein NADH:Ubiquinone oxidoreductase / Subunit NUIM of protein NADH:Ubiquinone oxidoreductase / Subunit NUHM of protein NADH:Ubiquinone oxidoreductase / NUGM protein / NUCM protein / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUAM protein / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2
Similarity search - Component
Biological speciesYarrowia lipolytica (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.04 Å
AuthorsParey K / Vonck J
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research FoundationZI 552/4-2 Germany
CitationJournal: Sci Adv / Year: 2019
Title: High-resolution cryo-EM structures of respiratory complex I: Mechanism, assembly, and disease.
Authors: Kristian Parey / Outi Haapanen / Vivek Sharma / Harald Köfeler / Thomas Züllig / Simone Prinz / Karin Siegmund / Ilka Wittig / Deryck J Mills / Janet Vonck / Werner Kühlbrandt / Volker Zickermann /
Abstract: Respiratory complex I is a redox-driven proton pump, accounting for a large part of the electrochemical gradient that powers mitochondrial adenosine triphosphate synthesis. Complex I dysfunction is ...Respiratory complex I is a redox-driven proton pump, accounting for a large part of the electrochemical gradient that powers mitochondrial adenosine triphosphate synthesis. Complex I dysfunction is associated with severe human diseases. Assembly of the one-megadalton complex I in the inner mitochondrial membrane requires assembly factors and chaperones. We have determined the structure of complex I from the aerobic yeast by electron cryo-microscopy at 3.2-Å resolution. A ubiquinone molecule was identified in the access path to the active site. The electron cryo-microscopy structure indicated an unusual lipid-protein arrangement at the junction of membrane and matrix arms that was confirmed by molecular simulations. The structure of a complex I mutant and an assembly intermediate provide detailed molecular insights into the cause of a hereditary complex I-linked disease and complex I assembly in the inner mitochondrial membrane.
History
Header (metadata) releaseJul 18, 2018-
Map releaseOct 10, 2018-
DepositionApr 16, 2019-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6rfs
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4874.png

Downloads & links

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Map

FileDownload / File: emd_4874.map.gz / Format: CCP4 / Size: 361.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 456 pix.
= 497.04 Å		1.09 Å/pix.
x 456 pix.
= 497.04 Å		1.09 Å/pix.
x 456 pix.
= 497.04 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.09 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.014 / Movie #1: 0.014
Minimum - Maximum-0.014301295 - 0.09945536
Average (Standard dev.)0.000031120722 (±0.00156795)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions456456456
Spacing456456456
CellA=B=C: 497.04 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.091.091.09
M x/y/z456456456
origin x/y/z0.0000.0000.000
length x/y/z497.040497.040497.040
α/β/γ90.00090.00090.000
start NX/NY/NZ-51-35-11
NX/NY/NZ11110799
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS456456456
D min/max/mean-0.0140.0990.000

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Supplemental data

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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUMM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: ZINC ION
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 79.088078 KDa
SequenceString: MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE ...String:
MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE DKNIGPLVKT SMNRCIHCTR CVRFANDIAG APELGSSGRG NDMQIGTYLE KNLNTELSGN VIDLCPVGAL TN KPYAFRA RPWELKKTES IDVMDAVGSN IRIDSKGVEV MRVIPRVHED VNEEWINDKS RFACDGLKTQ RLTTPLIRVG DKF VNATWD DALSTIAKAY QQKAPKGDEF KAVAGALVEV ESMVALKDMT NALGSENTTT DTPNGNSAPA HGITFRSNYL FNSS IAGIE DADAILLVGT NPRREAAVMN ARIRKAWLRQ ELEIASVGPT LDATFDVAEL GNTHADLEKA LSGEFGEVLK NAKNP LIIV GSGITDREDA GAFFNTIGKF VESTPSVLNE NWNGYNVLQR SASRAGAYDI GFTPSDEASK TTPKMVWLLG ADEVAA SDI PADAFVVYQG HNGDVGAQFA DVVLPGAAYT EKAGTYVNTE GRSQISRAAT GPPGGAREDW KILRAVSEYL GVALPYE DA YEVRDRLAEI SPSLVRYDLV EPTVFGDVAV QHSLVGPNGS VTPSSAPLTE TIENFYMTDS ISRSSPTMAK SSIAFNKD N KKNQAFA

UniProtKB: NUAM protein

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Macromolecule #2: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.829508 KDa
SequenceString: MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA ...String:
MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA AVLQTAINEA YAAGLIGKDA CGSGYDFDVY IHRGMGAYVC GEETSLIESL EGKAGKPRLK PPFPAGVGLF GR PSTVTNV ETVAVAPTIL RRGGDWFASF GRERNSGTKL FCISGNVNEP CTVEEEMSIP LRELLEKHCG GIKGGWDNLL GVI PGGCSV PILPKNICED VLMDFDALKD VQSGLGTAAV IVINKQQDVI RAIQRFAAFY KHESCGQCTP CREGTTWLLK AMDR FRTGQ AKEREIDMLY ELTKDIEGHT ICALGDAAAW PIQGLIRNFR PEMETRMKKF HDEVGAVSVG GWMKDARVEK GKVVG APLP GVHH

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

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Macromolecule #3: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 52.494906 KDa
SequenceString: MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL ...String:
MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL RGKYIRTMFG EITRVLNHLM SVCSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVSQD LP AGLLDDI YMWATQFGDR LDEIEELLTD NRIWKLRTVN IGTVTAQDAL NLGLSGPMLR GSGIPFDIRK NAPYDAYDKV DFD VPVGMN GDCYDRYLIR MAEFRQSLRI IEQCCNDMPA GAVKVEDFKI NSPPRNLMKE DMEALIHHFL LYTKGYSVPP GETY TAIEA PKGEMGVYVV SDGSERPYKC KIRAPGFAHL GAFDHIARGH FLPDAVAIIG TMDLVFGEVD R

UniProtKB: NUCM protein

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Macromolecule #4: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.806238 KDa
SequenceString:
MAIPFEALLP YGIIFGLLTA GGGAMQVLHV YRNGGVRDRF AIDQWDSQMM ERDLRLNGGQ GRKQVDQATA PEAFKHNHVW KSERPLI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

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Macromolecule #5: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 42.765738 KDa
SequenceString: MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA ...String:
MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA FNAEIDSPSE FNHTKGLGEQ VTKDIVPWAT IVRPAPMFGR EDKWFLDRMA RSPCLVSANK FQETSNPVHV ID VAAALER ICFDDSTVAQ TFELYGPQKF TQKQIIDMVS ETLRKEVRHI ELPKALYQAY TKATQAIWWP TYSPDQVERQ FLS QKIDPS AKTFNDLDLT PMELPDLMFK LIRPYRVNTF QHDVSQLENK EKTFVHILD

UniProtKB: NAD-dependent epimerase/dehydratase domain-containing protein

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Macromolecule #6: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.65792 KDa
SequenceString:
MRYTQLLRNV SKGVQKSGQS VLVQMREGTP IGLTGIYQHP NPRPALIALY EATLKELQDK HPKDSVYRQS IENLTAHRKQ IVEDNEVSE VIENKIGAGL IEEVVIQAHE ELELAKKMSE WKPWEELEEK PLEDQWVYFN KKGVE

UniProtKB: ETC complex I subunit conserved region-domain-containing protein

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Macromolecule #7: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 32.389568 KDa
SequenceString: MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI ...String:
MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI TCLYEGANWF EREAYDMYGV FFEGHPDLRR IMTDYGFEGH PLRKDFPLTG YTEVRWDEEK RRVVYEPLEL TQ AFRNFSA GSTAWEPVGP GRDDRPDSFK LPTPKPEEKE GDKK

UniProtKB: NUGM protein

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Macromolecule #8: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 27.247402 KDa
SequenceString: MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC ...String:
MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC LGACVNAPMM AINDDYYEDL TPEGTVKLLE DCKAGKMPTP GPENHVRRDC EPASGQKVLL SKEPHNVADF LQ EGI

UniProtKB: Subunit NUHM of protein NADH:Ubiquinone oxidoreductase

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Macromolecule #9: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 25.682305 KDa
SequenceString: MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK ...String:
MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK CIYCGYCQES CPVDAIVETP NVEYATETRE ELLYNKEKLL ANGDKWELEL QYALDADAPY R

UniProtKB: Subunit NUIM of protein NADH:Ubiquinone oxidoreductase

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Macromolecule #10: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.849551 KDa
SequenceString: MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH ...String:
MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH RRPLSLTVEQ LGEGRGINAV PIATATEAPN

UniProtKB: Uncharacterized protein

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Macromolecule #11: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 23.455176 KDa
SequenceString: MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD ...String:
MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD RIVPVDVYVP GCPPTSEALM YGVFQLQRKM RNTKITRMWY RK

UniProtKB: Subunit NUKM of protein NADH:Ubiquinone oxidoreductase

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Macromolecule #12: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.815851 KDa
SequenceString:
MFIGTIILVL SFLGFVFNRR NIILAFICLE TMLLGINLIL LRNSVLFDDI SGSLFAIVII ILAGVESAIG LSLLVSYYRL RGVINSYGI

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

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Macromolecule #13: Subunit NUMM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUMM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 15.14897 KDa
SequenceString:
MLSRFVSKRA FSVSTQVSKS IISYNGNTIE IPEEYTKQAP NRDSTWAPAQ ASKTEIYKNN MVRFEQKDLS KQPMPYAGIE LIAQQPVRF VHGNTAVCDG ANHNGPGAQG HPKIFINVDA PGSHACQYCG TRYEKEH

UniProtKB: Zinc finger CHCC-type domain-containing protein

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Macromolecule #14: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 12.053585 KDa
SequenceString:
MLRNVSRLAL RSNFARQATM GQRFYSVARP DAEKRIAAVL ESFDKISNPA AITPTASFAK DLNLDSLDTV EVVVAIEEEF GIEIPDKEA DEIKSVNQAV EYILAQPDAK

UniProtKB: Acyl carrier protein

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Macromolecule #15: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.778818 KDa
SequenceString:
MAIIATAFAE TVKFSGSKQE LQKRTLALYR QFLRGAPTFA DLYEVQFSIP TIRTKIRQEF ERHRFVDDLS IQNVLYAKGH MEYQECINF WKQQAQFLKY FPEEDDIQGR HQPSNFVDKF LKNRA

UniProtKB: Complex 1 LYR protein domain-containing protein

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Macromolecule #16: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.444458 KDa
SequenceString:
MLRQSVLRLS RAAVARPALS RSFVTAVARP QLVRAAPVSF IRHYSSAHVL TKDMIQERIV ALLESFDKVN DAKNITATAN LTSDLGLDS LDVVEVVMAI EEEFGLEIPD HDADEIKTVQ QAIDYVSAQP AAV

UniProtKB: Acyl carrier protein

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Macromolecule #17: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 12.90287 KDa
SequenceString:
MSHPVPFSAA NKKLVTSMYR QSLKLARNWI SNRQLFRQKA VEIRHKFDQN AQISNPRLLA RTLDETRAHL YEFRHPDPIV PPSFPGGTK YERNVPPRME KIMQHNLYEP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

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Macromolecule #18: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 28.479941 KDa
SequenceString: MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM ...String:
MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM YFLYAPSLIL LLIVMVYKSR EDITFWAKKE LDQRVLDKHP EINDAPENER DALIVERIIA GDYDKLASLQ KK ATPTPAT LI

UniProtKB: NUWM protein

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Macromolecule #19: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.355197 KDa
SequenceString:
MPREAAAHWV PFEDKANMPD NVPDVVEVGA TSAPLLSASY FIGAKCKPYN DDFMLCREES QGSGAIDCLK EGRRVTRCAV SVIEDINKS CLDEFRLHWQ CLEQNNHQLS GCRKAEALLN KCVFTKLNLE KKIPGLRPDE EPVFLKKDPW IKPAVDDFKS V RAYAEAKK NGTL

UniProtKB: NADH-ubiquinone oxidoreductase

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Macromolecule #20: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.112429 KDa
SequenceString:
MPSVGQDLPP VGGYEPVQWR RNLPARGFRP LVYLAALCGI CGYGFYRALG GIQERRELKR EKLWARIYLM PLLQAEEDRQ TVRRSIAQL EREKEIMKGT GFDVDKSVYN DGKFHAPALM IPPK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

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Macromolecule #21: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 18.588209 KDa
SequenceString:
MSSSTPLVKT SVNYSYGDYP LIDADPHFKR VVGYMRPSDY GVIGLATAAL PAGICFAEWL DPVKGKFARP SVKFLRVATM LGFAVGFGA AYARSSLRFF GVTENAREYK KDEAQMAARK AAGLEPYGTS SLTPELQEIA AKNSAHSIAG LFIFPWFNFV N HPYHGREQ K

UniProtKB: NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein

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Macromolecule #22: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.772525 KDa
SequenceString:
MLPGGPVPVF KKYTVGSKGI WEKLRVLLAI APNRSTGNPI VPLYRVPTPG SRPEANVYQD PSSYPTNDIA ENPYWKRDHR RAYPQTAFF DQKTVTGLLE LGSEATPRIA DGEAGTKALA NIANGGVSFT QALGKSSKDV IYGEVLTVNG LPPVAPTLAP K QWKIIEGE AAIYPKGYPC RTFH

UniProtKB: Uncharacterized protein

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Macromolecule #23: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 17.328523 KDa
SequenceString:
MLSRRAVTML RSPVARVAQV QVRGIRASFD KAEEPMLGDY PDIDPFPAQL KNPYKKYDDQ QDRRNLEEPL SVNDDLYDMW SPDRFTHFK NQDALKYFIG FLTIFFGASY VATYFVPEKA AIPREFPYEG LWKESGGTEK SKAFFGQRSE

UniProtKB: Uncharacterized protein

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Macromolecule #24: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.059366 KDa
SequenceString:
MALFTSLVGA SGLGFATKFL SNKIRLKPAG YYPLGYVFSG VAWAGLGLVL HNVHQHSLEV LEKKKTALSE QRTE

UniProtKB: Uncharacterized protein

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Macromolecule #25: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 6.948902 KDa
SequenceString:
MAPQLKDPWA RREAWRYQTN FTRANRFKGA APGFGIAVVA FGAYLAAEKF LFEKKDDHHH

UniProtKB: NADH dehydrogenase 1 beta subcomplex subunit 3

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Macromolecule #26: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 11.039276 KDa
SequenceString:
MSEHQRPELV SFDDINYNDH KKVREAQESY TREQFIRLEA LKTVRKALEK CYEESGPNHF EDCKNLAEQY LDMLPTHRLQ GYLGYQRND PSK

UniProtKB: Uncharacterized protein

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Macromolecule #27: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 7.807067 KDa
SequenceString:
MAGHHTNYKW NGPLPRPHVS AGHRIATKFL GATMFFWMFL RIKEEYPAML GLHNFYPPKE GEKAVEH

UniProtKB: Uncharacterized protein

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Macromolecule #28: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.621051 KDa
SequenceString:
MSAALREIRF HLCQNGSSSA PLRQFVKNQI GAFQKANPST KVLVREANGV KPIVFARFDH GHESKIGLDV SSEKEVAERV KSLIEAK

UniProtKB: Thioredoxin-like protein

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Macromolecule #29: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.921152 KDa
SequenceString:
MINANPGFWN GPFRYLRWSA HNRPHLFFAF AIGIAGPVAA LTLTPLRRKY LYPDHSPLPQ SYPLPQRARE GLTGFDDE

UniProtKB: Uncharacterized protein

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Macromolecule #30: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.175141 KDa
SequenceString:
MSSSLYRVLR NAWEVGPRSY WKQLNSIGDT KSGRLVGTDI YGNKFYETDH QDEIHLRTRY VEYKEKDYDM SQVEPGWHFW LGYGVDTAP CNTPKEKLPI RAYPYKFQPN YTGTPGAFVT YNTLKPKISA WEPVTKQRS

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit

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Macromolecule #31: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.793966 KDa
SequenceString:
MGGGRYPFPK DVISMTGGWW ANPSNWKLNG LFATGIAVGL ALWVSTATLP YTRRREGITS ESDISKWNAA AGVWRERHGK ISTGEAAES E

UniProtKB: Uncharacterized protein

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Macromolecule #32: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 10.494885 KDa
SequenceString:
MVELKPSSAI QRGPLNKGGW DAPHALHNDG AIDRYAHWRT FYQERFKYTR ATGKSTLIFL VAFPALIGYV AYQSEGLFEF AGKRRGESV TTRG

UniProtKB: NUVM protein

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Macromolecule #33: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 13.523068 KDa
SequenceString:
FGSHGHGPEL TPAVPFFQPY VLKWAGVSLG LVAFYQFNSS YEAKNGHTWV ETFFHPKSRE DILNEEAKIV QALNNQRELT IKMHELKRE EKDYAHSYSP LFSDPVPQGG SIGKAPGSSR E

UniProtKB: Uncharacterized protein

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Macromolecule #34: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 38.361266 KDa
SequenceString: MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT ...String:
MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT IIETQRVVWY CIPLLPLLLI FFIASVAETA RPPFDLTESE SELVAGYFTE YSGSPFVFFF LAEYSNIILI SA FNGYLLL GGYLSFNYSY LFNILFNDYS YVSFLFEGLI NSSAYAIKLV FLMFSFIWVR AAFPRFTYDN LINFCWIILL PLL FGIFLI IPSTLYIFDS FPTLI

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

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Macromolecule #35: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.353359 KDa
SequenceString: MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI ...String:
MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI AYSINTYYSV LNSYTLHSLD SLIINTLDLN LILIALSLGL LFKIGIAPLH KWLISIYENT PILITIYISL IP KISILSY LVLSNISINS LVISILAILT LLVGSVGGLL QIKIKRLLAF SGLTNAGYMM LLLLLNNNEF SYLYYITQYS ISH LAIFMI IIFSIYYINY INNQYNPIIY VNQLKGLIHD NAYLVLSMAI VVFSFIGIPP LLGFFGKLNI LMSILNNGYY FISI VLIVA SLISALYYLY LLNVSIQDKN NILINSNETV SSVLSYILSS LIILITFGFI YNSLIIDIFN VYFN

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

+
Macromolecule #36: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.478329 KDa
SequenceString:
MNTFIIFIIL IPIVGFALLA VNILLAVYKP YNEKLGAFEC GLTSFNQTRL AFNAAFILVA ILFLPFDLEI STLLPYVMSI YLVSNYGFT IVLLFLLILI IGFVYEINTN ALKINKHNKP NTDSLIYKL

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

+
Macromolecule #37: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 54.506641 KDa
SequenceString: MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL ...String:
MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL MFTLSGSLFM LLSIVVISIV LNTTNFINHN LFVLSLDLQT IIWLGLFIAI MVKTPLFPIH VWLPVVHSES PL AGSMILA GLILKLALYA ILRLLLPLLC EAQILYTPMI YIISLLTIIL TSLATLRQID LKVIIAYSSI SHMGIAILGV CSN TSLGIY GSIVLGVAHG FVSPALFLIV GGILYDRYHI RIVNYYKGLT TYMPQLATYI IILSFANIGT PLTGNFTGEF LSLQ GGFIR NPIIGGISCI SVLLAAIYQL KLTNKLTGGI SSIYMHRTND VTIREKFIMN ILIISTLIIG ICPQIMYNLL YWTVN NYIY II

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

+
Macromolecule #38: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 73.740688 KDa
SequenceString: MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL ...String:
MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL SAVLMNRFGD AFFVLGLCVI AYVFGTLNYS TIFATAYLIN TDLLVLIMLA LFIAAMAKSA QFGLHNWLTL AM EGPTPVS SLLHAATLVT AGIYLLLRSA NILEYTPTVL FIILWIGALT TLSAGLIAIC SNDLKRIIAL STMSQLGMMT IAI GLSAYN LALFHLLGHA FFKALLFMSA GSIIHSILNE SQDIRTYGGL LSYLPYTYIC ITIASLSLMA MPGLTGYYTK DIII ESTYG SYSISNYVVY WIAYLSAVLT CVYSMKILYL TFYSNPNNNT ITYYNAHESN IYITLPMFIL AIFAMFAGWI LKDIY LGVG TDFVGTHILP NNFSYFDTEF SITQFYKLLP LISAILVSIL IVVLNEFFAI VFNLNNKYIN TVYSIFNQKL VSDQIL NHF IIFKGLVTSG NIAHHVDKGS LYRLGPVGIN RLLNKASYNV INLSSNTRSS LSMNSMLILI TIVSLLLLVL VMNVNFI IV IPVLISILYI LFS

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

+
Macromolecule #39: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.765102 KDa
SequenceString:
MMYLTYYFIE ITIFLAILCT IFIISAKNPM VSILYMIALF VIAAMYLYLI GLGIFSLLYI MIYIGAIAVL FLFIITLLDI NSTELSVKS NIRDLPLVLI SLIVLTISGL MIYSNDSILI NKLLEAFGND YNTIITQDWF NIENTTLLTT IGNVLLTNNA F ILLVLAIV LLLGIIGPIS ITMKHKE

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

+
Macromolecule #40: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 11.219027 KDa
SequenceString:
MAEFPPLLSQ EDMKKHKILL AYRDRCAALL VPLNECRKKN YYMPWACGHE RHEYEMCEVA DFQRRVKAMD KLKAEKIEQA KAAAAAAAA AAAADAEESK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

+
Macromolecule #41: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 10.035651 KDa
SequenceString:
MTAAGISLTG GRNRCFSEWQ SFMHCTAKTD AKSRAQCLPN FEDYMECLHH TKEKARLREI ESVLKQKKEG LEAPPVKVIP VKAIGLVEE

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

+
Macromolecule #42: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 42 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #43: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 43 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

+
Macromolecule #44: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 44 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

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Macromolecule #45: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 45 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

+
Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #47: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 47 / Number of copies: 1 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2.7 mg/mL
BufferpH: 7.2
Component:
ConcentrationNameFormula
20.0 mMTris
100.0 mMSodium ChlorideNaCl
1.0 mMEDTA
0.025 %DDM
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI POLARA 300
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 1 / Number real images: 5964 / Average exposure time: 8.0 sec. / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 45872 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: -3.0 µm / Nominal defocus min: -2.0 µm / Nominal magnification: 200000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 418740
Startup modelType of model: EMDB MAP
EMDB ID:

4384

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.04 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 145767
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final 3D classificationNumber classes: 3 / Software - Name: RELION (ver. 3.0)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-6rfs:
Cryo-EM structure of a respiratory complex I mutant lacking NDUFS4

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