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- EMDB-4873: Cryo-EM structure of respiratory complex I from Yarrowia lipolyti... -

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Basic information

Entry
Database: EMDB / ID: EMD-4873
TitleCryo-EM structure of respiratory complex I from Yarrowia lipolytica at 3.2 A resolution
Map datacomplex I from Yarrowia lipolyticaRespiratory complex I
Sample
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: x 42 types
  • Ligand: x 13 types
Function / homology
Function and homology information


lipoate biosynthetic process / NADH dehydrogenase / TIM23 mitochondrial import inner membrane translocase complex / mitochondrial iron-sulfur cluster assembly complex / protein import into mitochondrial matrix / ubiquinone-6 biosynthetic process / NADH dehydrogenase activity / respiratory chain complex I / cellular respiration / oxidoreductase activity, acting on NAD(P)H ...lipoate biosynthetic process / NADH dehydrogenase / TIM23 mitochondrial import inner membrane translocase complex / mitochondrial iron-sulfur cluster assembly complex / protein import into mitochondrial matrix / ubiquinone-6 biosynthetic process / NADH dehydrogenase activity / respiratory chain complex I / cellular respiration / oxidoreductase activity, acting on NAD(P)H / ubiquinone binding / acyl binding / electron transport coupled proton transport / iron-sulfur cluster assembly / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / respirasome / aerobic respiration / respiratory electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / mitochondrial inner membrane / response to oxidative stress / oxidoreductase activity / protein-containing complex binding / mitochondrion / membrane / metal ion binding
Similarity search - Function
NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / Soluble ligand binding domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 ...NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / Soluble ligand binding domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / SLBB domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / GRIM-19 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NDUFA6, LYR domain / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH dehydrogenase, subunit C / NADH ubiquinone oxidoreductase, F subunit / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / 2Fe-2S iron-sulfur cluster binding domain / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / CHCH / NuoE domain / CHCH domain / NADH:ubiquinone oxidoreductase / NADH-quinone oxidoreductase subunit E-like / Thioredoxin-like [2Fe-2S] ferredoxin / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter / NADH-quinone oxidoreductase subunit E, N-terminal / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH:ubiquinone oxidoreductase, 51kDa subunit, conserved site / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2.
Similarity search - Domain/homology
Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein ...Complex 1 LYR protein domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein / Acyl carrier protein / Uncharacterized protein / Uncharacterized protein / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-domain-containing protein / Uncharacterized protein / Thioredoxin-like protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / Uncharacterized protein / Zinc finger CHCC-type domain-containing protein / NAD-dependent epimerase/dehydratase domain-containing protein / NADH dehydrogenase 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase / ETC complex I subunit conserved region-domain-containing protein / Uncharacterized protein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / YALI0D10274p / YALI0E23749p / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / YALI0A17946p / YALI0F06061p / YALI0F18359p / YALI0F00924p / YALI0F17248p / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / YALI0E29095p / YALI0D00737p / YALI0F02123p / YALI0D05467p / YALI0F14003p / YALI0F06050p / YALI0E28424p / YALI0E23089p / YALI0E11891p / Acyl carrier protein / YALI0D24585p / YALI0D19030p / Acyl carrier protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / YALI0D04939p / YALI0C03201p / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit N7BM / NADH-ubiquinone oxidoreductase / YALI0A02651p / YALI0A01419p / NUWM protein / NUVM protein / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 6 / Subunit NUKM of protein NADH:Ubiquinone oxidoreductase / Subunit NUIM of protein NADH:Ubiquinone oxidoreductase / Subunit NUHM of protein NADH:Ubiquinone oxidoreductase / NUGM protein / NUCM protein / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUAM protein / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2
Similarity search - Component
Biological speciesYarrowia lipolytica (yeast) / Candida lipolytica (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsParey K / Vonck J
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research FoundationZI 552/4-2 Germany
CitationJournal: Sci Adv / Year: 2019
Title: High-resolution cryo-EM structures of respiratory complex I: Mechanism, assembly, and disease.
Authors: Kristian Parey / Outi Haapanen / Vivek Sharma / Harald Köfeler / Thomas Züllig / Simone Prinz / Karin Siegmund / Ilka Wittig / Deryck J Mills / Janet Vonck / Werner Kühlbrandt / Volker Zickermann /
Abstract: Respiratory complex I is a redox-driven proton pump, accounting for a large part of the electrochemical gradient that powers mitochondrial adenosine triphosphate synthesis. Complex I dysfunction is ...Respiratory complex I is a redox-driven proton pump, accounting for a large part of the electrochemical gradient that powers mitochondrial adenosine triphosphate synthesis. Complex I dysfunction is associated with severe human diseases. Assembly of the one-megadalton complex I in the inner mitochondrial membrane requires assembly factors and chaperones. We have determined the structure of complex I from the aerobic yeast by electron cryo-microscopy at 3.2-Å resolution. A ubiquinone molecule was identified in the access path to the active site. The electron cryo-microscopy structure indicated an unusual lipid-protein arrangement at the junction of membrane and matrix arms that was confirmed by molecular simulations. The structure of a complex I mutant and an assembly intermediate provide detailed molecular insights into the cause of a hereditary complex I-linked disease and complex I assembly in the inner mitochondrial membrane.
History
Header (metadata) releaseJul 18, 2018-
Map releaseOct 10, 2018-
DepositionApr 16, 2019-
UpdateJun 2, 2021-
Current statusJun 2, 2021Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6rfr
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4873.png

Downloads & links

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Map

FileDownload / File: emd_4873.map.gz / Format: CCP4 / Size: 361.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcomplex I from Yarrowia lipolytica
Voxel sizeX=Y=Z: 1.077 Å
Density
Contour LevelBy AUTHOR: 0.2 / Movie #1: 0.02
Minimum - Maximum-0.11926842 - 0.27606687
Average (Standard dev.)5.8969376e-06 (±0.004038026)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions456456456
Spacing456456456
CellA=B=C: 491.112 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0771.0771.077
M x/y/z456456456
origin x/y/z0.0000.0000.000
length x/y/z491.112491.112491.112
α/β/γ90.00090.00090.000
start NX/NY/NZ-51-35-11
NX/NY/NZ11110799
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS456456456
D min/max/mean-0.1190.2760.000

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Supplemental data

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Sample components

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Entire : Mitochondrial NADH:ubiquinone oxidoreductase

EntireName: Mitochondrial NADH:ubiquinone oxidoreductase
Components
  • Complex: Mitochondrial NADH:ubiquinone oxidoreductase
    • Protein or peptide: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
    • Protein or peptide: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: CARDIOLIPIN
  • Ligand: Lauryl Maltose Neopentyl Glycol
  • Ligand: DIUNDECYL PHOSPHATIDYL CHOLINE
  • Ligand: ZINC ION
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: Ubiquinone-9Coenzyme Q10
  • Ligand: Phosphatidylinositol
  • Ligand: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE

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Supramolecule #1: Mitochondrial NADH:ubiquinone oxidoreductase

SupramoleculeName: Mitochondrial NADH:ubiquinone oxidoreductase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#42
Source (natural)Organism: Yarrowia lipolytica (yeast) / Strain: GB30
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUAM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 79.088078 KDa
SequenceString: MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE ...String:
MLSRNLSKFA RAGLIRPATT STHTRLFSVS ARRLAEIELT IDGHKVSIEA GSALIQACEK AGVTVPRYCY HDKLAIAGNC RMCLVDVER APKPVASCAY PVAPGMVVRT DTERVKQARE NVMEMMLQNH PLDCPVCDQG GECDLQDQSM RYGRDRGRFT E ITGKRSTE DKNIGPLVKT SMNRCIHCTR CVRFANDIAG APELGSSGRG NDMQIGTYLE KNLNTELSGN VIDLCPVGAL TN KPYAFRA RPWELKKTES IDVMDAVGSN IRIDSKGVEV MRVIPRVHED VNEEWINDKS RFACDGLKTQ RLTTPLIRVG DKF VNATWD DALSTIAKAY QQKAPKGDEF KAVAGALVEV ESMVALKDMT NALGSENTTT DTPNGNSAPA HGITFRSNYL FNSS IAGIE DADAILLVGT NPRREAAVMN ARIRKAWLRQ ELEIASVGPT LDATFDVAEL GNTHADLEKA LSGEFGEVLK NAKNP LIIV GSGITDREDA GAFFNTIGKF VESTPSVLNE NWNGYNVLQR SASRAGAYDI GFTPSDEASK TTPKMVWLLG ADEVAA SDI PADAFVVYQG HNGDVGAQFA DVVLPGAAYT EKAGTYVNTE GRSQISRAAT GPPGGAREDW KILRAVSEYL GVALPYE DA YEVRDRLAEI SPSLVRYDLV EPTVFGDVAV QHSLVGPNGS VTPSSAPLTE TIENFYMTDS ISRSSPTMAK SSIAFNKD N KKNQAFA

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Macromolecule #2: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUBM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 53.829508 KDa
SequenceString: MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA ...String:
MLRTTLHKRG LARLSRGFAT TQDATPKARQ YGGLKDQDRI FQNLYDNYGW DLASARKQGD WYKTKELILK GDTWIIDEIK KSGLRGRGG AGFPSGLKWS FMNPPGWEKN EGPRYLVVNA DEGEPGTCKD REIMRKDPHK LVEGCLLAGR AMNATAAYIY I RGEFYNEA AVLQTAINEA YAAGLIGKDA CGSGYDFDVY IHRGMGAYVC GEETSLIESL EGKAGKPRLK PPFPAGVGLF GR PSTVTNV ETVAVAPTIL RRGGDWFASF GRERNSGTKL FCISGNVNEP CTVEEEMSIP LRELLEKHCG GIKGGWDNLL GVI PGGCSV PILPKNICED VLMDFDALKD VQSGLGTAAV IVINKQQDVI RAIQRFAAFY KHESCGQCTP CREGTTWLLK AMDR FRTGQ AKEREIDMLY ELTKDIEGHT ICALGDAAAW PIQGLIRNFR PEMETRMKKF HDEVGAVSVG GWMKDARVEK GKVVG APLP GVHH

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Macromolecule #3: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUCM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 52.494906 KDa
SequenceString: MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL ...String:
MLRSAAARAV RAVRPRLSAR YMATTALPQD PIPSGALGQK VPHVDESHQD LLFRTSHMVE DLETYDEDSP INTSDANTRI RAFTINFGP QHPAAHGVLR LILELSGEEI IRSDPHVGLL HRGTEKLIEY KTYMQALPYF DRLDYVSMMT NEQVFSLAVE K LLNVEVPL RGKYIRTMFG EITRVLNHLM SVCSHAMDVG ALTPFLWGFE EREKLMEFYE RVSGARLHAA YVRPGGVSQD LP AGLLDDI YMWATQFGDR LDEIEELLTD NRIWKLRTVN IGTVTAQDAL NLGLSGPMLR GSGIPFDIRK NAPYDAYDKV DFD VPVGMN GDCYDRYLIR MAEFRQSLRI IEQCCNDMPA GAVKVEDFKI NSPPRNLMKE DMEALIHHFL LYTKGYSVPP GETY TAIEA PKGEMGVYVV SDGSERPYKC KIRAPGFAHL GAFDHIARGH FLPDAVAIIG TMDLVFGEVD R

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Macromolecule #4: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 9.806238 KDa
SequenceString:
MAIPFEALLP YGIIFGLLTA GGGAMQVLHV YRNGGVRDRF AIDQWDSQMM ERDLRLNGGQ GRKQVDQATA PEAFKHNHVW KSERPLI

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Macromolecule #5: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUEM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 42.765738 KDa
SequenceString: MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA ...String:
MLRTTRAVKP LKAITTSVRF MNSFENLAQD VNITRSGKTL IAKGTGGRSS RTGYTATVFG ANGFLGSYLT AKLAKHGTTV VVPYREEMA KRHLKVTGDL GVVNFLEMDL RNLESIDEAV RHSDIVVNLI GREYETKNFN YYDVHVEGAR RIAEAVKKHN I ARYIHVSA FNAEIDSPSE FNHTKGLGEQ VTKDIVPWAT IVRPAPMFGR EDKWFLDRMA RSPCLVSANK FQETSNPVHV ID VAAALER ICFDDSTVAQ TFELYGPQKF TQKQIIDMVS ETLRKEVRHI ELPKALYQAY TKATQAIWWP TYSPDQVERQ FLS QKIDPS AKTFNDLDLT PMELPDLMFK LIRPYRVNTF QHDVSQLENK EKTFVHILD

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Macromolecule #6: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUFM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.65792 KDa
SequenceString:
MRYTQLLRNV SKGVQKSGQS VLVQMREGTP IGLTGIYQHP NPRPALIALY EATLKELQDK HPKDSVYRQS IENLTAHRKQ IVEDNEVSE VIENKIGAGL IEEVVIQAHE ELELAKKMSE WKPWEELEEK PLEDQWVYFN KKGVE

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Macromolecule #7: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUGM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 32.389568 KDa
SequenceString: MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI ...String:
MLSRFARIGS MGIRPVAAAR ATFVTSARAA QAAPSWENIK DIRLDPKVHV DEVYEPIVNP ADRYLQHVSD LHQYAKYIMA ALPKYIQGF SVWKDELTLH VAPSAVIPVT TFLRDNTSTQ YKSIIDITAV DYPSRENRFE VVYNFLSVRH NSRIRLKTYA T EVTPVPSI TCLYEGANWF EREAYDMYGV FFEGHPDLRR IMTDYGFEGH PLRKDFPLTG YTEVRWDEEK RRVVYEPLEL TQ AFRNFSA GSTAWEPVGP GRDDRPDSFK LPTPKPEEKE GDKK

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Macromolecule #8: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUHM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 27.247402 KDa
SequenceString: MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC ...String:
MLRLIRPRLA ALARPTTRAP QALNARTHIV SVHRNTENNN PSIPFEFSPE NMKRAEEVIA KYPPQYKKAA VMPLLDIGQR QLGYTSISV MNYVAKLLEM PPMRVYEVAT FYTMYNRTPM GRYHLQICTT TPCQLCGSDG IMEAVQNTLN IKPGETTKDN L FTLSEVEC LGACVNAPMM AINDDYYEDL TPEGTVKLLE DCKAGKMPTP GPENHVRRDC EPASGQKVLL SKEPHNVADF LQ EGI

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Macromolecule #9: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUIM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 25.682305 KDa
SequenceString: MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK ...String:
MLSLVRPAVT RSILRGAPGS MRLLSSTARL HAPATDSAIN IYAGGSAAAA PPAGFRIHRP ATWEESEEGA LSKATKYFLL AEMFRGLYV VLEQFFRAPY TIYYPFEKGP VSPRFRGEHA LRRYPSGEER CIACKLCEAI CPALAITIDA EERIDGSRRT T KYDIDMTK CIYCGYCQES CPVDAIVETP NVEYATETRE ELLYNKEKLL ANGDKWELEL QYALDADAPY R

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Macromolecule #10: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUJM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.849551 KDa
SequenceString: MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH ...String:
MSEHVHLPSP TFNTSSAGHF HPYNTLGLAT QSAMVGLGAG VVAAAARNSL ATGPRNILTT FSKSGGVVTI FTGASIAYVF TYCSAANLR ERKDGWNHMW AGAATGAVLG ARTKLVPAFI GWTVLCGAAC GLFGWTGARF NADRKASLEQ SPKGFVQEDA H QTFWEVVH RRPLSLTVEQ LGEGRGINAV PIATATEAPN

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Macromolecule #11: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUKM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 23.455176 KDa
SequenceString: MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD ...String:
MLRSQIGRLA LRPTLVPATV IPQTRAYSAP AGTPRVSSSS MPTDFPLPSQ QKPNSAVDYT LTTLDAVANW ARQGSFWPVT FGLACCAVE MMHVSAPRYD QDRLGIIFRA SPRQSDIMIV AGTLTNKMAP VLRQVYDQMP EPRWVISMGS CANGGGYYHF S YSVVRGCD RIVPVDVYVP GCPPTSEALM YGVFQLQRKM RNTKITRMWY RK

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Macromolecule #12: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NULM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.815851 KDa
SequenceString:
MFIGTIILVL SFLGFVFNRR NIILAFICLE TMLLGINLIL LRNSVLFDDI SGSLFAIVII ILAGVESAIG LSLLVSYYRL RGVINSYGI

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Macromolecule #13: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUMM of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 15.14897 KDa
SequenceString:
MLSRFVSKRA FSVSTQVSKS IISYNGNTIE IPEEYTKQAP NRDSTWAPAQ ASKTEIYKNN MVRFEQKDLS KQPMPYAGIE LIAQQPVRF VHGNTAVCDG ANHNGPGAQG HPKIFINVDA PGSHACQYCG TRYEKEH

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Macromolecule #14: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM1 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 12.053585 KDa
SequenceString:
MLRNVSRLAL RSNFARQATM GQRFYSVARP DAEKRIAAVL ESFDKISNPA AITPTASFAK DLNLDSLDTV EVVVAIEEEF GIEIPDKEA DEIKSVNQAV EYILAQPDAK

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Macromolecule #15: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB4M of protein NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.778818 KDa
SequenceString:
MAIIATAFAE TVKFSGSKQE LQKRTLALYR QFLRGAPTFA DLYEVQFSIP TIRTKIRQEF ERHRFVDDLS IQNVLYAKGH MEYQECINF WKQQAQFLKY FPEEDDIQGR HQPSNFVDKF LKNRA

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Macromolecule #16: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Com...

MacromoleculeName: Acyl carrier protein ACPM2 of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.444458 KDa
SequenceString:
MLRQSVLRLS RAAVARPALS RSFVTAVARP QLVRAAPVSF IRHYSSAHVL TKDMIQERIV ALLESFDKVN DAKNITATAN LTSDLGLDS LDVVEVVMAI EEEFGLEIPD HDADEIKTVQ QAIDYVSAQP AAV

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Macromolecule #17: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 12.90287 KDa
SequenceString:
MSHPVPFSAA NKKLVTSMYR QSLKLARNWI SNRQLFRQKA VEIRHKFDQN AQISNPRLLA RTLDETRAHL YEFRHPDPIV PPSFPGGTK YERNVPPRME KIMQHNLYEP

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Macromolecule #18: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NESM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 28.479941 KDa
SequenceString: MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM ...String:
MLKLHYRNFI TAQHSTTNTT PTMIASVCKR AGLRAGPRAY PGVRQFALRA YNEEKELALK QRLSQLPPPG KAFVTAEGEP RPAKEAELA ELAEIAALYK TDRVGILDIL LLGNKHARLY RDNTALLKDY YYNGRRILDK IPVKDKQTGK VTWEIKREGA E KEDWVNQM YFLYAPSLIL LLIVMVYKSR EDITFWAKKE LDQRVLDKHP EINDAPENER DALIVERIIA GDYDKLASLQ KK ATPTPAT LI

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Macromolecule #19: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.355197 KDa
SequenceString:
MPREAAAHWV PFEDKANMPD NVPDVVEVGA TSAPLLSASY FIGAKCKPYN DDFMLCREES QGSGAIDCLK EGRRVTRCAV SVIEDINKS CLDEFRLHWQ CLEQNNHQLS GCRKAEALLN KCVFTKLNLE KKIPGLRPDE EPVFLKKDPW IKPAVDDFKS V RAYAEAKK NGTL

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Macromolecule #20: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB6M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 14.112429 KDa
SequenceString:
MPSVGQDLPP VGGYEPVQWR RNLPARGFRP LVYLAALCGI CGYGFYRALG GIQERRELKR EKLWARIYLM PLLQAEEDRQ TVRRSIAQL EREKEIMKGT GFDVDKSVYN DGKFHAPALM IPPK

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Macromolecule #21: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUXM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 18.588209 KDa
SequenceString:
MSSSTPLVKT SVNYSYGDYP LIDADPHFKR VVGYMRPSDY GVIGLATAAL PAGICFAEWL DPVKGKFARP SVKFLRVATM LGFAVGFGA AYARSSLRFF GVTENAREYK KDEAQMAARK AAGLEPYGTS SLTPELQEIA AKNSAHSIAG LFIFPWFNFV N HPYHGREQ K

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Macromolecule #22: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUYM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 18.65608 KDa
SequenceString:
MLSRSLRQLS QPSVRSFATS ARLLQKKDVP EVGVNLDNVP AHEIVSGAPA ELSRNRVVRI YQQAKPATQS GEYGTFAWRL DWDIVDVAN RWENDLIGWQ SSGDYMQATQ MKFTSKESAI KFANKQGWDF YIQEPHHRKF RVKQYANNFV HSYGKLKHIR T K

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Macromolecule #23: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUZM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 19.772525 KDa
SequenceString:
MLPGGPVPVF KKYTVGSKGI WEKLRVLLAI APNRSTGNPI VPLYRVPTPG SRPEANVYQD PSSYPTNDIA ENPYWKRDHR RAYPQTAFF DQKTVTGLLE LGSEATPRIA DGEAGTKALA NIANGGVSFT QALGKSSKDV IYGEVLTVNG LPPVAPTLAP K QWKIIEGE AAIYPKGYPC RTFH

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Macromolecule #24: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIAM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 17.328523 KDa
SequenceString:
MLSRRAVTML RSPVARVAQV QVRGIRASFD KAEEPMLGDY PDIDPFPAQL KNPYKKYDDQ QDRRNLEEPL SVNDDLYDMW SPDRFTHFK NQDALKYFIG FLTIFFGASY VATYFVPEKA AIPREFPYEG LWKESGGTEK SKAFFGQRSE

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Macromolecule #25: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.059366 KDa
SequenceString:
MALFTSLVGA SGLGFATKFL SNKIRLKPAG YYPLGYVFSG VAWAGLGLVL HNVHQHSLEV LEKKKTALSE QRTE

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Macromolecule #26: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 6.948902 KDa
SequenceString:
MAPQLKDPWA RREAWRYQTN FTRANRFKGA APGFGIAVVA FGAYLAAEKF LFEKKDDHHH

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Macromolecule #27: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIDM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 11.039276 KDa
SequenceString:
MSEHQRPELV SFDDINYNDH KKVREAQESY TREQFIRLEA LKTVRKALEK CYEESGPNHF EDCKNLAEQY LDMLPTHRLQ GYLGYQRND PSK

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Macromolecule #28: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUVM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 7.807067 KDa
SequenceString:
MAGHHTNYKW NGPLPRPHVS AGHRIATKFL GATMFFWMFL RIKEEYPAML GLHNFYPPKE GEKAVEH

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Macromolecule #29: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 9.621051 KDa
SequenceString:
MSAALREIRF HLCQNGSSSA PLRQFVKNQI GAFQKANPST KVLVREANGV KPIVFARFDH GHESKIGLDV SSEKEVAERV KSLIEAK

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Macromolecule #30: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NI9M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 8.921152 KDa
SequenceString:
MINANPGFWN GPFRYLRWSA HNRPHLFFAF AIGIAGPVAA LTLTPLRRKY LYPDHSPLPQ SYPLPQRARE GLTGFDDE

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Macromolecule #31: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit N7BM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 16.175141 KDa
SequenceString:
MSSSLYRVLR NAWEVGPRSY WKQLNSIGDT KSGRLVGTDI YGNKFYETDH QDEIHLRTRY VEYKEKDYDM SQVEPGWHFW LGYGVDTAP CNTPKEKLPI RAYPYKFQPN YTGTPGAFVT YNTLKPKISA WEPVTKQRS

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Macromolecule #32: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUUM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 9.793966 KDa
SequenceString:
MGGGRYPFPK DVISMTGGWW ANPSNWKLNG LFATGIAVGL ALWVSTATLP YTRRREGITS ESDISKWNAA AGVWRERHGK ISTGEAAES E

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Macromolecule #33: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB5M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 10.494885 KDa
SequenceString:
MVELKPSSAI QRGPLNKGGW DAPHALHNDG AIDRYAHWRT FYQERFKYTR ATGKSTLIFL VAFPALIGYV AYQSEGLFEF AGKRRGESV TTRG

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Macromolecule #34: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NUNM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 13.523068 KDa
SequenceString:
FGSHGHGPEL TPAVPFFQPY VLKWAGVSLG LVAFYQFNSS YEAKNGHTWV ETFFHPKSRE DILNEEAKIV QALNNQRELT IKMHELKRE EKDYAHSYSP LFSDPVPQGG SIGKAPGSSR E

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Macromolecule #35: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU1M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 38.361266 KDa
SequenceString: MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT ...String:
MIINIVEILI FLVCVLFSVA YLTVAERKTL AYMQRRLGPN FVGYYGLLQA FADAVKLLLK EIVLPKESNY IILVISPLIT LITALIGWV VIPLGPGITL GELNLGILFS LAIGSLGVFG SLLSGWSSNS KYSLLGSIRS TAQLISYELI LTSIFIIIIM F VSSLNITT IIETQRVVWY CIPLLPLLLI FFIASVAETA RPPFDLTESE SELVAGYFTE YSGSPFVFFF LAEYSNIILI SA FNGYLLL GGYLSFNYSY LFNILFNDYS YVSFLFEGLI NSSAYAIKLV FLMFSFIWVR AAFPRFTYDN LINFCWIILL PLL FGIFLI IPSTLYIFDS FPTLI

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Macromolecule #36: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU2M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 53.353359 KDa
SequenceString: MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI ...String:
MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI FNSFTFYIDM LIYFIMIVIS SLYGYNLYN NNLYKTLFEP KKELIILFLI NILGALLIVH SNDFITLFVA IELQSYSIYL ITAIYNSSYK ASKASMLYFF M GGILSILI AYSINTYYSV LNSYTLHSLD SLIINTLDLN LILIALSLGL LFKIGIAPLH KWLISIYENT PILITIYISL IP KISILSY LVLSNISINS LVISILAILT LLVGSVGGLL QIKIKRLLAF SGLTNAGYMM LLLLLNNNEF SYLYYITQYS ISH LAIFMI IIFSIYYINY INNQYNPIIY VNQLKGLIHD NAYLVLSMAI VVFSFIGIPP LLGFFGKLNI LMSILNNGYY FISI VLIVA SLISALYYLY LLNVSIQDKN NILINSNETV SSVLSYILSS LIILITFGFI YNSLIIDIFN VYFN

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Macromolecule #37: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU3M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 14.478329 KDa
SequenceString:
MNTFIIFIIL IPIVGFALLA VNILLAVYKP YNEKLGAFEC GLTSFNQTRL AFNAAFILVA ILFLPFDLEI STLLPYVMSI YLVSNYGFT IVLLFLLILI IGFVYEINTN ALKINKHNKP NTDSLIYKL

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Macromolecule #38: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU4M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 54.506641 KDa
SequenceString: MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL ...String:
MFLTSILLSS LYLFNRILAW QGNVKHFYLF ASNLLLLFIV VLYINFNTFS NSFQFNFELF NSLNPFGLSN SDISNGLLFG IDGLSLTFI LLTVLLIPLT LLGNWYNINF NSNLYYTLVL AIGLVILLNF WALDYISFYI LFEATLPLLF ILIHIYGSSD S ERASFYVL MFTLSGSLFM LLSIVVISIV LNTTNFINHN LFVLSLDLQT IIWLGLFIAI MVKTPLFPIH VWLPVVHSES PL AGSMILA GLILKLALYA ILRLLLPLLC EAQILYTPMI YIISLLTIIL TSLATLRQID LKVIIAYSSI SHMGIAILGV CSN TSLGIY GSIVLGVAHG FVSPALFLIV GGILYDRYHI RIVNYYKGLT TYMPQLATYI IILSFANIGT PLTGNFTGEF LSLQ GGFIR NPIIGGISCI SVLLAAIYQL KLTNKLTGGI SSIYMHRTND VTIREKFIMN ILIISTLIIG ICPQIMYNLL YWTVN NYIY II

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Macromolecule #39: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU5M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 73.740688 KDa
SequenceString: MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL ...String:
MYNAISLIII LPCISWLFPL FFGRQLGYVF VTRMTSTLII ITTLITYYYF YQLLGNNNPI NLELFNYLNI DYLDINYNFE IDALTITML LAITTISSMV HIYSIGYMET DPHQVRFFSL LSMFTFWMII LVTGSNYFVL FVGWEFIGVT SYLLISFWVT R LQAMKSAL SAVLMNRFGD AFFVLGLCVI AYVFGTLNYS TIFATAYLIN TDLLVLIMLA LFIAAMAKSA QFGLHNWLTL AM EGPTPVS SLLHAATLVT AGIYLLLRSA NILEYTPTVL FIILWIGALT TLSAGLIAIC SNDLKRIIAL STMSQLGMMT IAI GLSAYN LALFHLLGHA FFKALLFMSA GSIIHSILNE SQDIRTYGGL LSYLPYTYIC ITIASLSLMA MPGLTGYYTK DIII ESTYG SYSISNYVVY WIAYLSAVLT CVYSMKILYL TFYSNPNNNT ITYYNAHESN IYITLPMFIL AIFAMFAGWI LKDIY LGVG TDFVGTHILP NNFSYFDTEF SITQFYKLLP LISAILVSIL IVVLNEFFAI VFNLNNKYIN TVYSIFNQKL VSDQIL NHF IIFKGLVTSG NIAHHVDKGS LYRLGPVGIN RLLNKASYNV INLSSNTRSS LSMNSMLILI TIVSLLLLVL VMNVNFI IV IPVLISILYI LFS

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Macromolecule #40: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NU6M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
Source (natural)Organism: Yarrowia lipolytica (yeast)
Molecular weightTheoretical: 20.765102 KDa
SequenceString:
MMYLTYYFIE ITIFLAILCT IFIISAKNPM VSILYMIALF VIAAMYLYLI GLGIFSLLYI MIYIGAIAVL FLFIITLLDI NSTELSVKS NIRDLPLVLI SLIVLTISGL MIYSNDSILI NKLLEAFGND YNTIITQDWF NIENTTLLTT IGNVLLTNNA F ILLVLAIV LLLGIIGPIS ITMKHKE

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Macromolecule #41: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NB8M of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 11.219027 KDa
SequenceString:
MAEFPPLLSQ EDMKKHKILL AYRDRCAALL VPLNECRKKN YYMPWACGHE RHEYEMCEVA DFQRRVKAMD KLKAEKIEQA KAAAAAAAA AAAADAEESK

+
Macromolecule #42: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)

MacromoleculeName: Subunit NIPM of NADH:Ubiquinone Oxidoreductase (Complex I)
type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Candida lipolytica (yeast)
Molecular weightTheoretical: 10.035651 KDa
SequenceString:
MTAAGISLTG GRNRCFSEWQ SFMHCTAKTD AKSRAQCLPN FEDYMECLHH TKEKARLREI ESVLKQKKEG LEAPPVKVIP VKAIGLVEE

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Macromolecule #43: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 43 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #44: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 44 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

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Macromolecule #45: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 45 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

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Macromolecule #46: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / type: ligand / ID: 46 / Number of copies: 15 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da

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Macromolecule #47: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 47 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

+
Macromolecule #48: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 48 / Number of copies: 6 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

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Macromolecule #49: Lauryl Maltose Neopentyl Glycol

MacromoleculeName: Lauryl Maltose Neopentyl Glycol / type: ligand / ID: 49 / Number of copies: 2 / Formula: LMN
Molecular weightTheoretical: 1.005188 KDa
Chemical component information

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

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Macromolecule #50: DIUNDECYL PHOSPHATIDYL CHOLINE

MacromoleculeName: DIUNDECYL PHOSPHATIDYL CHOLINE / type: ligand / ID: 50 / Number of copies: 7 / Formula: PLC
Molecular weightTheoretical: 622.834 Da
Chemical component information

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

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Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #52: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 52 / Number of copies: 2 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Macromolecule #53: Ubiquinone-9

MacromoleculeName: Ubiquinone-9 / type: ligand / ID: 53 / Number of copies: 1 / Formula: UQ9
Molecular weightTheoretical: 795.226 Da
Chemical component information

ChemComp-UQ9:
Ubiquinone-9

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Macromolecule #54: Phosphatidylinositol

MacromoleculeName: Phosphatidylinositol / type: ligand / ID: 54 / Number of copies: 4 / Formula: T7X
Molecular weightTheoretical: 887.128 Da
Chemical component information

ChemComp-T7X:
Phosphatidylinositol / Phosphatidylinositol

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Macromolecule #55: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 55 / Number of copies: 1 / Formula: CPL
Molecular weightTheoretical: 758.06 Da
Chemical component information

ChemComp-CPL:
1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2 mg/mL
BufferpH: 7.2
Component:
ConcentrationFormulaName
20.0 mMTris
100.0 mMNaClSodium chloridesodium cloride
1.0 mMEDTAEthylenediaminetetraacetic acid
0.025 %LMNG
GridModel: C-flat-1/1 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 46425 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: -2.5 µm / Nominal defocus min: -1.5 µm / Nominal magnification: 130000
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 4043 / Average exposure time: 8.0 sec. / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 666666
CTF correctionSoftware - Name: CTFFIND (ver. 4.1)
Startup modelType of model: EMDB MAP
EMDB ID:

4384

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final 3D classificationNumber classes: 3 / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 297066
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6gcs

RefinementSpace: REAL / Protocol: OTHER
Output model

PDB-6rfr:
Cryo-EM structure of respiratory complex I from Yarrowia lipolytica at 3.2 A resolution

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