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- EMDB-48269: Cryo-EM structure of G protein-gated inwardly rectifying potassiu... -

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Basic information

Entry
Database: EMDB / ID: EMD-48269
TitleCryo-EM structure of G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
Map data
Sample
  • Complex: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
    • Protein or peptide: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
    • Protein or peptide: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
KeywordsG protein gated-inwardly rectifying potassium channels / MEMBRANE PROTEIN
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.13 Å
AuthorsNguyen H / Mount J / Yuan P / Slesinger P
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism (NIH/NIAAA)5R01AA018734-13 United States
CitationJournal: To Be Published
Title: Key residue Unlocks Dual PIP2-Dependent and Independent Gating in G Protein-Gated Inwardly Rectifying Potassium Channels
Authors: Nguyen H / Mount J / Hutchinson K / Zhao Y / Zhao Y / Glaaser I / Yuan P / Schlessinger A / Slesinger P
History
DepositionDec 11, 2024-
Header (metadata) releaseDec 10, 2025-
Map releaseDec 10, 2025-
UpdateDec 10, 2025-
Current statusDec 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48269.png

Downloads & links

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Map

FileDownload / File: emd_48269.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 256 pix.
= 270.848 Å		1.06 Å/pix.
x 256 pix.
= 270.848 Å		1.06 Å/pix.
x 256 pix.
= 270.848 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.058 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.3
Minimum - Maximum-1.7424157 - 2.378906
Average (Standard dev.)0.00041335967 (±0.0415654)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 270.848 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_48269_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_48269_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)

EntireName: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
Components
  • Complex: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
    • Protein or peptide: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
    • Protein or peptide: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)

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Supramolecule #1: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)

SupramoleculeName: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)

MacromoleculeName: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Recombinant expressionOrganism: Komagataella pastoris (fungus)
SequenceString: MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWQFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE ...String:
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWQFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE GIILLLIQSV LGSIVNAFMV GCMFVKISQP KKRAETLVFS THAVISMRDG KLCLMFRVGD LRNSHIVEAS IR AKLIKSK QTSEGEFIPL NQTDINVGYY TGDDRLFLVS PLIISHEINQ QSPFWEISKA QLPKEELEIV VILEGMVEAT GMT CQARSS YITSEILWGY RFTPVLTLED GFYEVDYNSF HETYETSTPS LSAKELAELA NRAESPLSWS VSSKLNQHAE LETE EEEKN PEELTERNGD VANLENESKS

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Macromolecule #2: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)

MacromoleculeName: G protein-gated inwardly rectifying potassium channel 2 R92Q (State 2)
type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Recombinant expressionOrganism: Komagataella pastoris (fungus)
SequenceString: MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWQFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE ...String:
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWQFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE GIILLLIQSV LGSIVNAFMV GCMFVKISQP KKRAETLVFS THAVISMRDG KLCLMFRVGD LRNSHIVEAS IR AKLIKSK QTSEGEFIPL NQTDINVGYY TGDDRLFLVS PLIISHEINQ QSPFWEISKA QLPKEELEIV VILEGMVEAT GMT CQARSS YITSEILWGY RFTPVLTLED GFYEVDYNSF HETYETSTPS LSAKELAELA NRAESPLSWS VSSKLNQHAE LETE EEEKN PEELTERNGD VANLENESKS

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 14099 / Average electron dose: 51.73 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.1 µm / Nominal defocus min: 0.1 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.13 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 386612
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
Final 3D classificationNumber classes: 3
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT

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