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- EMDB-48268: CryoEM structure of G protein-gated inwardly rectifying potassium... -

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Basic information

Entry
Database: EMDB / ID: EMD-48268
TitleCryoEM structure of G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
Map data
Sample
  • Complex: G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
    • Protein or peptide: G protein-activated inward rectifier potassium channel 2
KeywordsG protein gated-inwardly rectifying potassium channels / MEMBRANE PROTEIN
Function / homology
Function and homology information


G-protein activated inward rectifier potassium channel activity / inward rectifier potassium channel complex
Similarity search - Function
Potassium channel, inwardly rectifying, Kir3.2 / Potassium channel, inwardly rectifying, transmembrane domain / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel, C-terminal / Inward rectifier potassium channel C-terminal domain / Immunoglobulin E-set
Similarity search - Domain/homology
G protein-activated inward rectifier potassium channel 2
Similarity search - Component
Biological speciesMus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.68 Å
AuthorsNguyen H / Mount J / Yuan P / Slesinger P
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism (NIH/NIAAA)5R01AA018734-13 United States
CitationJournal: To Be Published
Title: Key residue Unlocks Dual PIP2-Dependent and Independent Gating in G Protein-Gated Inwardly Rectifying Potassium Channels
Authors: Nguyen H / Mount J / Hutchinson K / Zhao Y / Zhao Y / Glaaser I / Yuan P / Schlessinger A / Slesinger P
History
DepositionDec 11, 2024-
Header (metadata) releaseDec 10, 2025-
Map releaseDec 10, 2025-
UpdateDec 10, 2025-
Current statusDec 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48268.png

Downloads & links

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Map

FileDownload / File: emd_48268.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 256 pix.
= 273.664 Å		1.07 Å/pix.
x 256 pix.
= 273.664 Å		1.07 Å/pix.
x 256 pix.
= 273.664 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.069 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.45
Minimum - Maximum-2.0011666 - 3.4411664
Average (Standard dev.)0.008898869 (±0.07016413)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 273.664 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_48268_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_48268_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)

EntireName: G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
Components
  • Complex: G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
    • Protein or peptide: G protein-activated inward rectifier potassium channel 2

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Supramolecule #1: G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)

SupramoleculeName: G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mus musculus (house mouse)

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Macromolecule #1: G protein-activated inward rectifier potassium channel 2

MacromoleculeName: G protein-activated inward rectifier potassium channel 2
type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse)
Molecular weightTheoretical: 48.685371 KDa
Recombinant expressionOrganism: Komagataella pastoris (fungus)
SequenceString: MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWFFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE ...String:
MTMAKLTESM TNVLEGDSMD QDVESPVAIH QPKLPKQARD DLPRHISRDR TKRKIQRYVR KDGKCNVHHG NVRETYRYLT DIFTTLVDL KWFFNLLIFV MVYTVTWLFF GMIWWLIAYI RGDMDHIEDP SWTPCVTNLN GFVSAFLFSI ETETTIGYGY R VITDKCPE GIILLLIQSV LGSIVNAFMV GCMFVKISQP KKRAETLVFS THAVISMRDG KLCLMFRVGD LRNSHIVEAS IR AKLIKSK QTSEGEFIPL NQTDINVGYY TGDDRLFLVS PLIISHEINQ QSPFWEISKA QLPKEELEIV VILEGMVEAT GMT CQARSS YITSEILWGY RFTPVLTLED GFYEVDYNSF HETYETSTPS LSAKELAELA NRAESPLSWS VSSKLNQHAE LETE EEEKN PEELTERNGD VANLENESKS

UniProtKB: G protein-activated inward rectifier potassium channel 2

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 20083 / Average electron dose: 53.92 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6xeu

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.68 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 782546
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
Final 3D classificationNumber classes: 3
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

6xeu


Chain - Chain ID: A / Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-9mh9:
CryoEM structure of G protein-gated inwardly rectifying potassium channel 2 R92F (State 1)

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