EMDB-47157: Yeast post-catalytic P complex spliceosome, state I, overall map

Basic information

Entry

Database: EMDB / ID: EMD-47157

• Title: Yeast post-catalytic P complex spliceosome, state I, overall map
• Map data: P-complex spliceosome state I core refinement map, postprocessed

Sample

• Complex: Yeast post-catalytic P complex spliceosome
  • RNA: x 5 types
  • Protein or peptide: x 32 types
• Ligand: x 6 types

• Keywords: spliceosome / snRNA / yeast / ribonucleoprotein complex / SPLICING

Function / homology

Function:

U2-type post-spliceosomal complex / mRNA branch site recognition / spliceosomal complex disassembly / post-spliceosomal complex / U2-type post-mRNA release spliceosomal complex / cellular bud site selection / pre-mRNA 3'-splice site binding / post-mRNA release spliceosomal complex / nuclear mRNA surveillance / generation of catalytic spliceosome for first transesterification step / cis assembly of pre-catalytic spliceosome / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / pre-mRNA binding / snRNP binding / small nuclear ribonucleoprotein complex / splicing factor binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / commitment complex / mRNA cis splicing, via spliceosome / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / poly(U) RNA binding / U2-type prespliceosome / precatalytic spliceosome / generation of catalytic spliceosome for second transesterification step / Formation of TC-NER Pre-Incision Complex / spliceosomal complex assembly / mRNA 5'-splice site recognition / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA 3'-splice site recognition / DNA replication origin binding / Dual incision in TC-NER / spliceosomal tri-snRNP complex assembly / Prp19 complex / protein K63-linked ubiquitination / U5 snRNA binding / DNA replication initiation / U5 snRNP / U2 snRNA binding / U6 snRNA binding / pre-mRNA intronic binding / spliceosomal snRNP assembly / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / positive regulation of cell cycle / catalytic step 2 spliceosome / nuclear periphery / positive regulation of RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / RING-type E3 ubiquitin transferase / double-strand break repair via nonhomologous end joining / metallopeptidase activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / chromosome, telomeric region / RNA helicase activity / RNA helicase / DNA repair / mRNA binding / GTPase activity / chromatin binding / chromatin / GTP binding / ATP hydrolysis activity / mitochondrion / DNA binding / RNA binding / zinc ion binding / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm

Domain/homology:

FAM192A/Fyv6, N-terminal / FAM192A/Fyv6, N-terminal domain / Prp18 / Pre-mRNA-splicing factor 18 / Prp18 domain / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / : / : / Pre-mRNA-splicing factor Isy1 / Pre-mRNA-splicing factor Isy1 superfamily / Isy1-like splicing family / cwf21 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / Torus domain / mRNA splicing factor SYF2 / SYF2 splicing factor / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / Helix hairpin bin domain superfamily / : / : / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / : / Prp19/Pso4-like / : / Pre-mRNA-splicing factor SYF1 middle HAT repeat / G10 protein signature 2. / BUD31/G10-related, conserved site / G10 protein signature 1. / : / : / STL11, N-terminal / : / Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / : / Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat / Pre-mRNA-splicing factor Cwf15/Cwc15 / Cwf15/Cwc15 cell cycle control protein / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / U2 small nuclear ribonucleoprotein A' / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / U-box domain profile. / Modified RING finger domain / U-box domain / Leucine-rich repeat / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Pre-mRNA-splicing factor Syf1-like / : / Helicase associated domain (HA2), ratchet-like / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Helicase associated domain (HA2) Add an annotation / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm6/SmF / Myb-type HTH DNA-binding domain profile. / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Myb domain / :

Component:

Pre-mRNA-splicing factor ISY1 / Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 / Pre-mRNA-splicing factor BUD31 / Pre-mRNA-processing protein 45 / Pre-mRNA-processing factor 19 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor 18 / Pre-mRNA-splicing factor SNU114 / Pre-mRNA-splicing factor SLT11 / Pre-mRNA-splicing factor NTC20 / Small nuclear ribonucleoprotein-associated protein B / Small nuclear ribonucleoprotein G / U2 small nuclear ribonucleoprotein B'' / Pre-mRNA-processing factor 17 / Small nuclear ribonucleoprotein Sm D3 / Pre-mRNA-splicing factor SYF2 / Pre-mRNA-splicing factor CWC22 / Protein FYV6 / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein Sm D1 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor CWC21 / Pre-mRNA-splicing factor CEF1 / Pre-mRNA-splicing factor CWC15 / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor SNT309 / Small nuclear ribonucleoprotein Sm D2 / U2 small nuclear ribonucleoprotein A' / Pre-mRNA-splicing factor CWC2 / Pre-mRNA-splicing factor CLF1 / Small nuclear ribonucleoprotein E / Pre-mRNA-splicing factor PRP46

• Biological species: Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 2.31 Å
• Authors: Wilkinson ME / Hoskins AA

Funding support

United States, 1 items

Organization	Grant number	Country
Howard Hughes Medical Institute (HHMI)	HHMI Helen Hay Whitney Postdoctoral Fellowship	United States

• Citation: Journal: bioRxiv / Year: 2024; Title: Control of 3' splice site selection by the yeast splicing factor Fyv6.; Authors: Katherine A Senn / Karli A Lipinski / Natalie J Zeps / Amory F Griffin / Max E Wilkinson / Aaron A Hoskins / ; Abstract: Pre-mRNA splicing is catalyzed in two steps: 5' splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (1 and 2 step factors). We recently identified Fyv6 (FAM192A in humans) as a 2 step factor in ; however, we did not determine how widespread Fyv6's impact is on the transcriptome. To answer this question, we have used RNA-seq to analyze changes in splicing. These results show that loss of Fyv6 results in activation of non-consensus, branch point (BP) proximal 3' SS transcriptome-wide. To identify the molecular basis of these observations, we determined a high-resolution cryo-EM structure of a yeast product complex spliceosome containing Fyv6 at 2.3 Å. The structure reveals that Fyv6 is the only 2 step factor that contacts the Prp22 ATPase and that Fyv6 binding is mutually exclusive with that of the 1 step factor Yju2. We then use this structure to dissect Fyv6 functional domains and interpret results of a genetic screen for suppressor mutations. The combined transcriptomic, structural, and genetic studies allow us to propose a model in which Yju2/Fyv6 exchange facilitates exon ligation and Fyv6 promotes usage of consensus, BP distal 3' SS.

History

Deposition	Oct 1, 2024	-
Header (metadata) release	Dec 25, 2024	-
Map release	Dec 25, 2024	-
Update	Jan 1, 2025	-
Current status	Jan 1, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_47157.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: P-complex spliceosome state I core refinement map, postprocessed
• Voxel size: X=Y=Z: 0.968 Å

Density

Contour Level	By AUTHOR: 0.02
Minimum - Maximum	-0.053846825 - 0.13771114
Average (Standard dev.)	-0.00023861734 (±0.003557824)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 464.63998 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_47157_msk_1.map

Half map: P-complex spliceosome state I core refinement half-map 2

• File: emd_47157_half_map_1.map
• Annotation: P-complex spliceosome state I core refinement half-map 2

Half map: P-complex spliceosome state I core refinement half-map 1

• File: emd_47157_half_map_2.map
• Annotation: P-complex spliceosome state I core refinement half-map 1

Sample components

Entire : Yeast post-catalytic P complex spliceosome

• Entire: Name: Yeast post-catalytic P complex spliceosome

Components

• Complex: Yeast post-catalytic P complex spliceosome
  • RNA: U2 snRNA
  • RNA: U5 snRNA
  • RNA: U6 snRNA
  • Protein or peptide: Pre-mRNA-splicing factor 8
  • Protein or peptide: Pre-mRNA-splicing factor SNU114
  • Protein or peptide: Protein FYV6
  • RNA: UBC4 mRNA spliced exons
  • Protein or peptide: Pre-mRNA-splicing factor ISY1
  • Protein or peptide: Pre-mRNA-splicing factor CWC22
  • RNA: UBC4 lariat-intron
  • Protein or peptide: Pre-mRNA-splicing factor PRP46
  • Protein or peptide: Pre-mRNA-processing protein 45
  • Protein or peptide: Pre-mRNA-splicing factor BUD31
  • Protein or peptide: Pre-mRNA-splicing factor CWC2
  • Protein or peptide: Pre-mRNA-splicing factor SLT11
  • Protein or peptide: Pre-mRNA-splicing factor CEF1
  • Protein or peptide: Pre-mRNA-splicing factor CWC15
  • Protein or peptide: Pre-mRNA-splicing factor CWC21
  • Protein or peptide: Pre-mRNA-splicing factor CLF1
  • Protein or peptide: Pre-mRNA-splicing factor SYF1
  • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
  • Protein or peptide: U2 small nuclear ribonucleoprotein A'
  • Protein or peptide: U2 small nuclear ribonucleoprotein B''
  • Protein or peptide: Pre-mRNA-splicing factor NTC20
  • Protein or peptide: Pre-mRNA-splicing factor 18
  • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
  • Protein or peptide: Pre-mRNA-splicing factor SLU7
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: Pre-mRNA-splicing factor SNT309
  • Protein or peptide: Pre-mRNA-processing factor 19
  • Protein or peptide: Pre-mRNA-splicing factor SYF2
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM ION
• Ligand: INOSITOL HEXAKISPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Yeast post-catalytic P complex spliceosome

• Supramolecule: Name: Yeast post-catalytic P complex spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#37
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 2.27 MDa

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 376.267406 KDa

Sequence

String:

ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGUUGU UUUCCGCUAA UGUCAGGUCU CACUACUUUU UGCUGCUAUU UUUCUUCGCU CAUGGUUUCU UCAUAAGGCG UU UUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU UCUGGUUGUU UUC UAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CUUUCCGAGC CGUU UAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGAUAAUAUU UAUGG UAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGGCGCUGC AAGAGG AAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGUAAUAG GAUAAGC UU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGGUGUA GGGUGGGG U UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUUUAU UUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGAA GAGCCAUGAC U GCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUGC CG GCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC ACUUGUGGAG UCG UUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

GENBANK: GENBANK: NR_132154.1

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 68.643344 KDa

Sequence

String:

AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

GENBANK: GENBANK: NR_132184.1

Macromolecule #3: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 35.883176 KDa

Sequence

String:

GUUCGCGAAG UAACCCUUCG UGGACAU(PSU)UG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAA GGA UGAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

GENBANK: GENBANK: NR_132206.1

Macromolecule #7: UBC4 mRNA spliced exons

• Macromolecule: Name: UBC4 mRNA spliced exons / type: rna / ID: 7 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 13.44003 KDa

Sequence

String:

GAACUAAGUG AUCUAGAAAG AGAUCCACCU ACUUCAUGUU GC

GENBANK: GENBANK: NM_001178430.1

Macromolecule #10: UBC4 lariat-intron

• Macromolecule: Name: UBC4 lariat-intron / type: rna / ID: 10 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 30.20073 KDa

Sequence

String:

GUAUGUCUAA AGUUAUGGCC ACGUUUCAAA UGCGUGCUUU UUUUUUAAAA CUUAUGCUCU UAUUUACUAA CAAAAUCAAC AUGCUAUUG AACUAG

GENBANK: GENBANK: CP059523.2

Macromolecule #4: Pre-mRNA-splicing factor 8

• Macromolecule: Name: Pre-mRNA-splicing factor 8 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 279.867469 KDa

Sequence

String:

MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

UniProtKB: Pre-mRNA-splicing factor 8

Macromolecule #5: Pre-mRNA-splicing factor SNU114

• Macromolecule: Name: Pre-mRNA-splicing factor SNU114 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 114.174008 KDa

Sequence

String:

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

UniProtKB: Pre-mRNA-splicing factor SNU114

Macromolecule #6: Protein FYV6

• Macromolecule: Name: Protein FYV6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.066938 KDa

Sequence

String:

MSSTSDNNAN SAREKKPLKF VSEGVGNVEA QRIREQVEQK KYEAEYKRKT RKSLRDQLRS NAISKQKQYN GLVRDRESFT RLSKEDLEF YQKSKNELLK KEKELNNYLD VKAINFEKKK KALLMENDST TNTEKYLETG TSLGSKTQIK GVKTSSPKPK I KVSIKKLG RKLEN

UniProtKB: Protein FYV6

Macromolecule #8: Pre-mRNA-splicing factor ISY1

• Macromolecule: Name: Pre-mRNA-splicing factor ISY1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.073836 KDa

Sequence

String:

MSRNVDKANS VLVRFQEQQA ESAGGYKDYS RYQRPRNVSK VKSIKEANEW KRQVSKEIKQ KSTRIYDPSL NEMQIAELND ELNNLFKEW KRWQWHIDHT LMEKKTKRKR LEDSHVLMNS GKLINGKRYF GRALELPEVK EWLKQSQRQN DGGSINTKCI P KDRNDFYY HGKVTAALTE FEANWTSILK AHYNVPVNED EEEMSRQTQE IHVPTLADME HWLVQRRKKK LMDELNL

UniProtKB: Pre-mRNA-splicing factor ISY1

Macromolecule #9: Pre-mRNA-splicing factor CWC22

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 67.386062 KDa

Sequence

String:

MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK THDMIWEKLR YILQTQELSS TLRESLETLF EIRQKDYKSG SQGLFILDPT SYTVHTHSYI VSDEDEANKE LG NFEKCEN FNELTMAFDT LRQKLLINNT SDTNEGSNSQ LQIYDMTSTN DVEFKKKIYL VLKSSLSGDE AAHKLLKLKI ANN LKKSVV DIIIKSSLQE STFSKFYSIL SERMITFHRS WQTAYNETFE QNYTQDIEDY ETDQLRILGK FWGHLISYEF LPMD CLKII KLTEEESCPQ GRIFIKFLFQ ELVNELGLDE LQLRLNSSKL DGMFPLEGDA EHIRYSINFF TAIGLGLLTE DMRSR LTII QEVEDAEEEE KKLREEEELE KLRKKARESQ PTQGPKIHES RLFLQKDTRE NSRSRSPFTV ETRKRARSRT PPRGSR NHR NRSRTPPARR QRHR

UniProtKB: Pre-mRNA-splicing factor CWC22

Macromolecule #11: Pre-mRNA-splicing factor PRP46

• Macromolecule: Name: Pre-mRNA-splicing factor PRP46 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 50.771289 KDa

Sequence

String:

MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKD HDTHASAFVN KIFQPEVAEE LIVNRYEKLL SQRPEWHAPW KLSRVINGHL GWVRCVAIDP VDNEWFITGS N DTTMKVWD LATGKLKTTL AGHVMTVRDV AVSDRHPYLF SVSEDKTVKC WDLEKNQIIR DYYGHLSGVR TVSIHPTLDL IA TAGRDSV IKLWDMRTRI PVITLVGHKG PINQVQCTPV DPQVVSSSTD ATVRLWDVVA GKTMKVLTHH KRSVRATALH PKE FSVASA CTDDIRSWGL AEGSLLTNFE SEKTGIINTL SINQDDVLFA GGDNGVLSFY DYKSGHKYQS LATREMVGSL EGER SVLCS TFDKTGLRLI TGEADKSIKI WKQDETATKE SEPGLAWNPN LSAKRF

UniProtKB: Pre-mRNA-splicing factor PRP46

Macromolecule #12: Pre-mRNA-processing protein 45

• Macromolecule: Name: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 42.548727 KDa

Sequence

String:

MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA VSNWKNPNGY TVALERRVGK ALDNENNTIN DGFMKLSEAL ENADKKARQE IRSKMELKRL AMEQEMLAKE SK LKELSQR ARYHNGTPQT GAIVKPKKQT STVARLKELA YSQGRDVSEK IILGAAKRSE QPDLQYDSRF FTRGANASAK RHE DQVYDN PLFVQQDIES IYKTNYEKLD EAVNVKSEGA SGSHGPIQFT KAESDDKSDN YGA

UniProtKB: Pre-mRNA-processing protein 45

Macromolecule #13: Pre-mRNA-splicing factor BUD31

• Macromolecule: Name: Pre-mRNA-splicing factor BUD31 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 18.484502 KDa

Sequence

String:

MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADK LLIAKWRKTG YEKLCCLRCI QKNETNNGST CICRVPRAQL EEEARKKGTQ VSFHQCVHCG CRGCASTD

UniProtKB: Pre-mRNA-splicing factor BUD31

Macromolecule #14: Pre-mRNA-splicing factor CWC2

• Macromolecule: Name: Pre-mRNA-splicing factor CWC2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 38.486562 KDa

Sequence

String:

MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG DIDRIRYVES KNCGFVKFKY QANAEFAKEA MSNQTLLLPS DKEWDDRREG TGLLVKWANE DPDPAAQKRL QE ELKLESL NMMVHLINNN TNSAGTEVNN KNNERLDRTF PEASVDNVKK RLLPLDNGME SDDFIEKLKK VKKNISRENI SSK PSVGKL GGPLLDYLSS DED

UniProtKB: Pre-mRNA-splicing factor CWC2

Macromolecule #15: Pre-mRNA-splicing factor SLT11

• Macromolecule: Name: Pre-mRNA-splicing factor SLT11 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 40.98859 KDa

Sequence

String:

MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ANEVKNLEKY ASVDISHILK KLPLNESFLK NPSTKSFFLY NIDASIPEWK ITDTVSQLLG IKKWKDGNSL SL IVNHKAK CGGLRFQSSE LGERFVSKIS ETLVTPKGLK RGVLLIDRFR IFIIPWSSGF SAASFGTNTA ENIKLSLSLN KLI QLELGL SFPTKSTDNA KNDKKKTSKK VHKDRSKKSK PRANKLTI

UniProtKB: Pre-mRNA-splicing factor SLT11

Macromolecule #16: Pre-mRNA-splicing factor CEF1

• Macromolecule: Name: Pre-mRNA-splicing factor CEF1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 67.837773 KDa

Sequence

String:

MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT RKIRERMLEE SKRIAELQKR RELKQAGINV AIKKPKKKYG TDIDYNEDIV YEQAPMPGIY DTSTEDRQIK KK FEQFERK VNRKGLDGNK DKPSKKNKDK KRKHDENEHV EKAALGESTT LTDEYKKPKL ILSAPGTKQG KVTYKKKLES KRQ KLIEAQ ATGTVLTPKE LLPHDSGQED NERSNIKSGK QLKSRIRKFL VQMFASLPSP KNDFEIVLSE DEKEEDAEIA EYEK EFENE RAMNEEDNFI EPPSQNDAPR VSLVAVPLAY STLPIPEFKN NPQSAIDNKY NLLVANAINK EPHMVPEDTV DFLKE VESR MQHITQGRTS MKIQFKTAMP PTEVLLESIQ SKVESIEQLQ RKLQHVQPLE QQNNEMCSTL CHHSLPALIE GQRKYY ADY YAYRQEIRSL EGRRKRLQAM LNSSSSI

UniProtKB: Pre-mRNA-splicing factor CEF1

Macromolecule #17: Pre-mRNA-splicing factor CWC15

• Macromolecule: Name: Pre-mRNA-splicing factor CWC15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 19.975195 KDa

Sequence

String:

MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND KSLEEAVMNE EKQDVVGSGN LQETRSEKD QKDSLQELLV TQKNKVEDKA ELEGNEQLKG GNSSRRSWRK GTAFGRHKVT KETNIKEHAT KKSASGYIND M TKSEYHQE FLHKHVR

UniProtKB: Pre-mRNA-splicing factor CWC15

Macromolecule #18: Pre-mRNA-splicing factor CWC21

• Macromolecule: Name: Pre-mRNA-splicing factor CWC21 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.793596 KDa

Sequence

String:

MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEET LSEEQIDKKC EALRAKLTNE WQEQQRMSSL YTPRKARLTE EQHRHE

UniProtKB: Pre-mRNA-splicing factor CWC21

Macromolecule #19: Pre-mRNA-splicing factor CLF1

• Macromolecule: Name: Pre-mRNA-splicing factor CLF1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 82.555859 KDa

Sequence

String:

MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA WNSFVDFEIR QKNWNGVREI YSKYVMAHPQ MQTWLKWVRF ENRHGNTEFT RSVYSLAIDT VANLQNLQIW SD MEVAKLV NSFAHWEAAQ QEYERSSALY QIAIEKWPSN QLLKAGLLDF EKQFGDINSI EETISYKRKM EYETILSNNA YDY DTWWLY LDLISESFPK QIMQTFEKAI VDSRPKELSK NVQWKRYIYL WMRYICYVEL ELENSLLEEE LFQRLIDDII PHKH FTFSK IWLMYAKFLI RHDDVPKARK ILGKAIGLCP KAKTFKGYIE LEVKLKEFDR VRKIYEKFIE FQPSDLQIWS QYGEL EENL GDWDRVRGIY TIALDENSDF LTKEAKIVLL QKYITFETES QEFEKARKLY RRYLELNQYS PQSWIEFAMY QTSTPT EQQ LLDLAKLQSE NVDEDIEFEI TDENKLEARK VFEEAIVFFK EKDDKQGRLS ILEALKDYEE TYGTELDQET VKKRFPK VI KKVRLQNGVE EEFVDYIFPD DIDDDKPKPS KFLELAKKWK QEQAL

UniProtKB: Pre-mRNA-splicing factor CLF1

Macromolecule #20: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 100.344016 KDa

Sequence

String:

MSAYIAMKGV ITNVDENIRN DEDVAFEYEI QKTPQNILTW KRYIEYWKEE GRTDKQIRWL YERFCSQFVT DTSIWEDYIR WESTKEVVE TSRIFWLFQR CLKSCVRDCD RICLSYLELA IEQYDLAMIR HALASSLMKM EREMHRKVWD PVIKFVEEKV L PLTQLDST QEDEEESTDE AELINVLLVK GFTKGGFISE EISENGSRGD IWSSHILERY LKVAPQQKRN ESLATLALTR DN ITIKSVY EKYLPQDENS GKYLPSSELP FELNFNYLAS LEKLGLDNQY EEFMRQMNGI YPDKWLFLIL SLAKYYISRG RLD SCGDLL KKSLQQTLRY SDFDRIYNFY LLFEQECSQF ILGKLKENDS KFFNQKDWTE KLQAHMATFE SLINLYDIYL NDVA LRQDS NLVETWMKRV SLQKSAAEKC NVYSEAILKI DPRKVGTPGS FGRLWCSYGD LYWRSNAIST ARELWTQSLK VPYPY IEDL EEIYLNWADR ELDKEGVERA FSILEDALHV PTNPEILLEK YKNGHRKIPA QTVLFNSLRI WSKYIDYLEA YCPKDA NSS DKIFNKTKMA YNTVIDLRLI TPAMAENFAL FLQNHYEVME SFQVYEKTIP LFPPEIQYEL WIEYLEVATS HQLSSLS PE HIRFLFEKAL KNLCSNGIDC KTIFIAYSVF EERISGLISK SIEILRRGAV IGTVSVSTHL ESRLQLWRMC ISKAESTL G PSVTRELYQE CIQILPNSKA VEFVIKFSDF ESSIGETIRA REILAYGAKL LPPSRNTELW DSFEIFELKH GDKETYKDM LKMKKVLESN MLIDSASVSH EEGNINFVAA ATSHAPNSHT LTQSTSSYSI NPDEIELDI

UniProtKB: Pre-mRNA-splicing factor SYF1

Macromolecule #21: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

• Macromolecule: Name: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22; type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 130.171359 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE DSIKMYNAFL GKQEEEKVRN KVKSSPLSQK INQVLKDDV NLDDPVVTEF VLSILNKSKS ITEFQEQLNL MQSGLDNETI FKIYQIASPP VMKEEVSVLP STKIPAKIEA K IEEEVQKI ESLDPSPVLH KVYEGKVRNI TTFGCFVQIF GTRMKNCDGL VHISEMSDQR TLDPHDVVRQ GQHIFVEVIK IQ NNGKISL SMKNIDQHSG EIRKRNTESV EDRGRSNDAH TSRNMKNKIK RRALTSPERW EIRQLIASGA ASIDDYPELK DEI PINTSY LTAKRDDGSI VNGNTEKVDS KLEEQQRDET DEIDVELNTD DGPKFLKDQQ VKGAKKYEMP KITKVPRGFM NRSA INGSN AIRDHREEKL RKKREIEQQI RKQQSFDDPT KNKKDSRNEI QMLKNQLIVT EWEKNRMNES ISYGKRTSLP ISAQR QTLP VYAMRSELIQ AVRDNQFLVI VGETGSGKTT QITQYLDEEG FSNYGMIGCT QPRRVAAVSV AKRVAEEVGC KVGHDV GYT IRFEDVTGPD TRIKYMTDGM LQREALLDPE MSKYSVIMLD EAHERTVATD VLFALLKKAA IKRPELKVIV TAATLNS AK FSEYFLNCPI INIPGKTFPV EVLYSQTPQM DYIEAALDCV IDIHINEGPG DILVFLTGQE EIDSCCEILY DRVKTLGD S IGELLILPVY SALPSEIQSK IFEPTPKGSR KVVFATNIAE TSITIDGIYY VVDPGFAKIN IYNARAGIEQ LIVSPISQA QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR QNLSHTILML KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEG KLTNLGKEMS LFPMDPTLSR SLLSSVDNQC SDEIVTIISM LSVQNVFYRP KDRQLEADSK KAKFHHPYGD H LTLLNVYT RWQQANYSEQ YCKTNFLHFR HLKRARDVKS QISMIFKKIG LKLISCHSDP DLIRKTFVSG FFMNAAKRDS QV GYKTING GTEVGIHPSS SLYGKEYEYV MYHSIVLTSR EYMSQVTSIE PQWLLEVAPH FYKAGDAESQ SRKKAKIIPL HNK FAKDQN SWRLSSIRQS RERALGIKR

UniProtKB: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

Macromolecule #22: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 27.232252 KDa

Sequence

String:

MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #23: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.850944 KDa

Sequence

String:

MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

UniProtKB: U2 small nuclear ribonucleoprotein B''

Macromolecule #24: Pre-mRNA-splicing factor NTC20

• Macromolecule: Name: Pre-mRNA-splicing factor NTC20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 15.992927 KDa

Sequence

String:

MPSLRDLSLE RDQELNQLRA RINQLGKTGK EEANDFVGLN ISNEPVYDTV IQTGQSSNAT NSFVQETIQK TKQKESGQPY IIPQKNEHQ RYIDKVCETS DLKAKLAPIM EVLEKKTNEK IKGIIRKRVL QEPDRDNDDS G

UniProtKB: Pre-mRNA-splicing factor NTC20

Macromolecule #25: Pre-mRNA-splicing factor 18

• Macromolecule: Name: Pre-mRNA-splicing factor 18 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 28.414391 KDa

Sequence

String:

MDLDLASILK GEISKKKKEL ANSKGVQPPC TEKFQPHESA NIDETPRQVE QESTDEENLS DNQSDDIRTT ISKLENRPER IQEAIAQDK TISVIIDPSQ IGSTEGKPLL SMKCNLYIHE ILSRWKASLE AYHPELFLDT KKALFPLLLQ LRRNQLAPDL L ISLATVLY HLQQPKEINL AVQSYMKLSI GNVAWPIGVT SVGIHARSAH SKIQGGRNAA NIMIDERTRL WITSIKRLIT FE EWYTSNH DSLA

UniProtKB: Pre-mRNA-splicing factor 18

Macromolecule #26: Small nuclear ribonucleoprotein-associated protein B

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 22.42699 KDa

Sequence

String:

MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

UniProtKB: Small nuclear ribonucleoprotein-associated protein B

Macromolecule #27: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 44.722875 KDa

Sequence

String:

MNNNSRNNEN RSTINRNKRQ LQQAKEKNEN IHIPRYIRNQ PWYYKDTPKE QEGKKPGNDD TSTAEGGEKS DYLVHHRQKA KGGALDIDN NSEPKIGMGI KDEFKLIRPQ KMSVRDSHSL SFCRNCGEAG HKEKDCMEKP RKMQKLVPDL NSQKNNGTVL V RATDDDWD SRKDRWYGYS GKEYNELISK WERDKRNKIK GKDKSQTDET LWDTDEEIEL MKLELYKDSV GSLKKDDADN SQ LYRTSTR LREDKAAYLN DINSTESNYD PKSRLYKTET LGAVDEKSKM FRRHLTGEGL KLNELNQFAR SHAKEMGIRD EIE DKEKVQ HVLVANPTKY EYLKKKREQE ETKQPKIVSI GDLEARKVDG TKQSEEQRNH LKDLYG

UniProtKB: Pre-mRNA-splicing factor SLU7

Macromolecule #28: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 11.240139 KDa

Sequence

String:

MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #29: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 10.385098 KDa

Sequence

String:

MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #30: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 9.669945 KDa

Sequence

String:

MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #31: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 8.490809 KDa

Sequence

String:

MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #32: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 16.296798 KDa

Sequence

String:

MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #33: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 12.876066 KDa

Sequence

String:

MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #34: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 52.128762 KDa

Sequence

String:

MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL KFLPKTGHLI LSGGNDHTIK IWDFYHDYEC LRDFQGHNKP IKALRFTEDC QSFLSSSFDR SVKIWDTETG KV KTRLHLN STPADVESRP TNPHEFIVGL SNSKILHYDD RVSENQGLVQ TYDHHLSSIL ALKYFPDGSK FISSSEDKTV RIW ENQINV PIKQISDTAQ HSMPFLNVHP SQNYFCAQSM DNRIYSFSLK PKYKRHPKKI FKGHSSAGYG ISLAFSGDGR YICS GDSKS RLFTWDWNTS RLLNNIKIPG NKPITQVDWH PQETSKVICS GAAGKIYVCD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #35: Pre-mRNA-splicing factor SNT309

• Macromolecule: Name: Pre-mRNA-splicing factor SNT309 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 20.741455 KDa

Sequence

String:

MDGLSFVDKG KIPDGYKNEI DQLVKKEFAN IKREPVHPEI RGILAKRKGA DNSVSTLTNA LYTEYLKQRN NKKRRTPDFN DDDDTLFLE EYRRKYPRID TSRYIPNESS EVSLLGIVDS YLKHQEIVLD TLLPQTVSNQ WRINNDYIRQ TCTIVEEMNI Q QRKQINDL EIYRKRL

UniProtKB: Pre-mRNA-splicing factor SNT309

Macromolecule #36: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 36 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 56.629777 KDa

Sequence

String:

MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK LKAPKWPILK NLELLQAQNY SRNIKTFPYK ELNKSMYYDK WVCMCRCEDG ALHFTQLKDS KTITTITTPN PR TGGEHPA IISRGPCNRL LLLYPGNQIT ILDSKTNKVL REIEVDSANE IIYMYGHNEV NTEYFIWADN RGTIGFQSYE DDS QYIVHS AKSDVEYSSG VLHKDSLLLA LYSPDGILDV YNLSSPDQAS SRFPVDEEAK IKEVKFADNG YWMVVECDQT VVCF DLRKD VGTLAYPTYT IPEFKTGTVT YDIDDSGKNM IAYSNESNSL TIYKFDKKTK NWTKDEESAL CLQSDTADFT DMDVV CGDG GIAAILKTND SFNIVALTP

UniProtKB: Pre-mRNA-processing factor 19

Macromolecule #37: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 24.850719 KDa

Sequence

String:

MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQ SQRGGISYDQ LAKLSYEKTL RNLATQTQNS SKQDSSADEE DNKNVPKKGR IGKVQKDTKT GKITIADDDK L VNKLAVSL QSESKKRYEA RKRQMQNAKT LYGVESFIND KNKQFNEKLS RESKGSE

UniProtKB: Pre-mRNA-splicing factor SYF2

Macromolecule #38: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 2 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #39: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 39 / Number of copies: 4 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #40: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 40 / Number of copies: 2 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

Macromolecule #41: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 41 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #42: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 42 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #43: water

• Macromolecule: Name: water / type: ligand / ID: 43 / Number of copies: 1 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.1 mg/mL

Buffer

pH: 7.9
Component:

Concentration	Name
20.0 mM	HEPES
75.0 mM	potassium chloride
0.25 mM	EDTA

• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 12 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 2 / Number real images: 51113 / Average exposure time: 0.72 sec. / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.1 µm / Nominal defocus min: 1.3 µm / Nominal magnification: 130000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1820457

Startup model

Type of model: EMDB MAP
EMDB ID:

10140

• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.31 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5.0) / Software - details: Blush regularization used / Number images used: 209005
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5.0)
• Final 3D classification: Number classes: 4 / Avg.num./class: 102236 / Software - Name: RELION (ver. 5.0)

Atomic model buiding 1

Initial model

PDB ID:

6exn

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Details: After fixing in ISOLDE, the model was refined in phenix.real_space_refine with the following non-default flags: refinement { run = *minimization_global rigid_body local_grid_search morphing simulated_annealing *adp } reference_model { enabled = True use_starting_model_as_reference = True fix_outliers = False strict_rotamer_matching = True } pdb_interpretation { reference_coordinate_restraints { enabled = True exclude_outliers = False top_out = True } automatic_linking { link_metals = True } peptide_link { restrain_rama_outliers = False restrain_rama_allowed = False } ramachandran_plot_restraints { enabled = False } }
• Refinement: Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-9dtr:
Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution