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- EMDB-4682: The cryo-EM structure of the tail knob in the bacteriophage phi29 -

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Basic information

Entry
Database: EMDB / ID: EMD-4682
TitleThe cryo-EM structure of the tail knob in the bacteriophage phi29
Map data
Sample
  • Virus: Bacillus phage phi29 (virus)
    • Protein or peptide: the tail knob protein (gp9, gene product 9) onf bacteriophage phi29
Biological speciesBacillus phage phi29 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.0 Å
AuthorsXu J / Wang D / Gui M / Xiang Y
Funding support China, 3 items
OrganizationGrant numberCountry
Ministry of Science and Technology (China)2016YFA0501100 China
National Natural Science Foundation of China31470721 China
Ministry of Science and Technology (China)2015CB910102 China
CitationJournal: Nat Commun / Year: 2019
Title: Structural assembly of the tailed bacteriophage ϕ29.
Authors: Jingwei Xu / Dianhong Wang / Miao Gui / Ye Xiang /
Abstract: The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile ...The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the ϕ29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-Å long, 24-strand β barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration.
History
DepositionMar 10, 2019-
Header (metadata) releaseJun 12, 2019-
Map releaseJun 12, 2019-
UpdateNov 6, 2019-
Current statusNov 6, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 1.3
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 1.3
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4682.png

Downloads & links

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Map

FileDownload / File: emd_4682.map.gz / Format: CCP4 / Size: 713.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.2952 Å
Density
Contour LevelBy AUTHOR: 1.3 / Movie #1: 1.3
Minimum - Maximum-0.42788726 - 12.099632
Average (Standard dev.)0.0015522873 (±0.050388884)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-287-287-287
Dimensions572572572
Spacing572572572
CellA=B=C: 740.8544 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.29519930069931.29519930069931.2951993006993
M x/y/z572572572
origin x/y/z0.0000.0000.000
length x/y/z740.854740.854740.854
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-287-287-287
NC/NR/NS572572572
D min/max/mean-0.42812.1000.002

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Supplemental data

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Sample components

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Entire : Bacillus phage phi29

EntireName: Bacillus phage phi29 (virus)
Components
  • Virus: Bacillus phage phi29 (virus)
    • Protein or peptide: the tail knob protein (gp9, gene product 9) onf bacteriophage phi29

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Supramolecule #1: Bacillus phage phi29

SupramoleculeName: Bacillus phage phi29 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 10756 / Sci species name: Bacillus phage phi29 / Virus type: PRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes

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Macromolecule #1: the tail knob protein (gp9, gene product 9) onf bacteriophage phi29

MacromoleculeName: the tail knob protein (gp9, gene product 9) onf bacteriophage phi29
type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Bacillus phage phi29 (virus)
SequenceString: MAYVPLSGTN VRILADVPFS NDYKNTRWFT SSSNQYNWFN RKSRVYEMSK VTFMGFRENK PYVSVSLPI DKLYSASYIM FQNADYGNKW FYAFVTELEF KNSAVTYVHF EIDVLQTWMF D MKFQESFI VREHVKLWND DGTPTINTID EGLSYGSEYD IVSVENHKPY ...String:
MAYVPLSGTN VRILADVPFS NDYKNTRWFT SSSNQYNWFN RKSRVYEMSK VTFMGFRENK PYVSVSLPI DKLYSASYIM FQNADYGNKW FYAFVTELEF KNSAVTYVHF EIDVLQTWMF D MKFQESFI VREHVKLWND DGTPTINTID EGLSYGSEYD IVSVENHKPY DDMMFLVIIS KS IMHGTPG EEESRLNDIN ASLNGMPQPL CYYIHPFYKD GKVPKTYIGD NNANLSPIVN MLT NIFSQK SAVNDIVNMY VTDYIGLKLD YKNGDKELKL DKDMFEQAGI ADDKHGNVDT IFVK KIPDY EALEIDTGDK WGGFTKDQES KLMMYPYCVT EITDFKGNHM NLKTEYINNS KLKIQ VRGS LGVSNKVAYS VQDYNADSAL SGGNRLTASL DSSLINNNPN DIAILNDYLS AYLQGN KNS LENQKSSILF NGIMGMIGGG ISAGASAAGG SALGMASSVT GMTSTAGNAV LQMQAMQ AK QADIANIPPQ LTKMGGNTAF DYGNGYRGVY VIKKQLKAEY RRSLSSFFHK YGYKINRV K KPNLRTRKAF NYVQTKDCFI SGDINNNDLQ EIRTIFDNGI TLWHTDNIGN YSVENELR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 5.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 36730

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