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- EMDB-45973: Bufavirus 1 at pH 2.6 -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-45973
TitleBufavirus 1 at pH 2.6
Map data
Sample
  • Virus: Bufavirus-1
    • Protein or peptide: VP1
KeywordsBufavirus / parvovirus / VIRUS LIKE PARTICLE
Function / homologyPhospholipase A2-like domain / Phospholipase A2-like domain / Parvovirus coat protein VP2 / Parvovirus coat protein VP1/VP2 / Parvovirus coat protein VP2 / Capsid/spike protein, ssDNA virus / T=1 icosahedral viral capsid / structural molecule activity / VP1
Function and homology information
Biological speciesBufavirus-1
Methodsingle particle reconstruction / cryo EM / Resolution: 2.73 Å
AuthorsGulkis MC / McKenna R / Bennett AD
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM082946 United States
CitationJournal: Viruses / Year: 2024
Title: Structural Characterization of Human Bufavirus 1: Receptor Binding and Endosomal pH-Induced Changes
Authors: Gulkis M / Luo M / Chipman P / Mietzsch M / Soderlund-Venermo M / Bennett A / McKenna R
History
DepositionJul 30, 2024-
Header (metadata) releaseAug 21, 2024-
Map releaseAug 21, 2024-
UpdateAug 21, 2024-
Current statusAug 21, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_45973.map.gz / Format: CCP4 / Size: 396.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)X (Row.)Y (Col.)
1.06 Å/pix.
x 470 pix.
= 496.32 Å
1.06 Å/pix.
x 470 pix.
= 496.32 Å
1.06 Å/pix.
x 470 pix.
= 496.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.056 Å
Density
Contour LevelBy AUTHOR: 2.0
Minimum - Maximum-15.1073 - 22.379899999999999
Average (Standard dev.)0.000000000759005 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderYXZ
Origin-235-235-235
Dimensions470470470
Spacing470470470
CellA=B=C: 496.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_45973_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: #1

Fileemd_45973_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Bufavirus-1

EntireName: Bufavirus-1
Components
  • Virus: Bufavirus-1
    • Protein or peptide: VP1

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Supramolecule #1: Bufavirus-1

SupramoleculeName: Bufavirus-1 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / Details: Bufavirus 1 VP2 was overexpressed in Sf9 cells / NCBI-ID: 1209382 / Sci species name: Bufavirus-1 / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes
Host (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 4 MDa
Virus shellShell ID: 1 / Diameter: 260.0 Å / T number (triangulation number): 1

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Macromolecule #1: VP1

MacromoleculeName: VP1 / type: protein_or_peptide / ID: 1 / Number of copies: 60 / Enantiomer: LEVO
Source (natural)Organism: Bufavirus-1
Molecular weightTheoretical: 80.543203 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MPAIRKARGW VPPGYNYLGP FNQDFNKKPT NPSDNAARKH DLEYNKLIKQ GHNPYWNYNH ADEDFIKETD QATDWGGKFG NFVFRAKRA LAPELAPPAK KKTKTKHTEP EYSHKHIKAG TKRGKPFYLF VNLARKKARM TDTQDVSEQQ SDQPSVASTN A KAGGGGGG ...String:
MPAIRKARGW VPPGYNYLGP FNQDFNKKPT NPSDNAARKH DLEYNKLIKQ GHNPYWNYNH ADEDFIKETD QATDWGGKFG NFVFRAKRA LAPELAPPAK KKTKTKHTEP EYSHKHIKAG TKRGKPFYLF VNLARKKARM TDTQDVSEQQ SDQPSVASTN A KAGGGGGG GGSGVGHSTG NYNNRTEFYY HGDEVTIVCH SSRHIHLNMS ESEEYKIYDT DRGPRFPTDQ TLQGRDTIND SY HAQVETP WFLINPNSWG TWMNPADFQQ LTTTCREVTL EHLDQTLDNI VIKTVSKQGS GAEETTQYNN DLTALLQVAL DKS NQLPWV ADNMYLDSLG YIPWRPCKLK QYSYHVNFWN TIDIISGPQQ NQWQQVKKEI RWDDLQFTPI ETTTEIDLLR TGDS WTSGP YKFNTKPTQL SYHWQSTRHT GSVHPTDPPN AIGQQGQNIR DINGWQWGDR SDPMSAATRV SNFHIGYSWP EWRIH YGSG GPAINPGAPF SQAPWSTDPQ VRLTQGASEK AIFDYNHGDD DPAHRDQWWQ NNLPITGQTN WAPKNAHQAN LSSNVP SRQ EFWTQDYHNT FGPFTAVDDV GIQYPWGAIW TKTPDTTHKP MMSAHAPFIC KDGPPGQLLV KLAPNYTENL QTDGLGN NR IVTYATFWWT GKLILKGKLR LPRQFNLYNL PGRPRGTEAK KFLPNEIGHF ELPFMPGRCM PNYTM

UniProtKB: VP1

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
50.0 mMC2H6ClNO2Glycine HCl
150.0 mMNaClNaCl
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 25 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Slit width: 20 eV
SoftwareName: Leginon
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-50 / Number real images: 1496 / Average electron dose: 75.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.9 µm / Nominal defocus min: 0.1 µm / Nominal magnification: 130000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 222998
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Resolution.type: BY AUTHOR / Resolution: 2.73 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.4.1) / Number images used: 121222
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4.1)
Final 3D classificationNumber classes: 4 / Software - Name: cryoSPARC (ver. 4.4.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

Chain - Source name: PDB / Chain - Initial model type: experimental model
SoftwareName: UCSF Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Overall B value: 166.7 / Target criteria: Cross-correlation coefficient
Output model


PDB Unreleased entry

PDB-9cws:
Bufavirus 1 at pH 2.6

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