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- EMDB-44782: D5E cage of PP7-AY-PP7: Pseudomonas phage PP7 coat protein dimer -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | D5E cage of PP7-AY-PP7: Pseudomonas phage PP7 coat protein dimer | |||||||||
![]() | sharpened b-factor: -66.5 | |||||||||
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![]() | VLP / PP7 / coat protein dimer / cage / C2 symmetry / VIRUS LIKE PARTICLE | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.74 Å | |||||||||
![]() | Kopylov M / Hernandez C / Bobe D / Keshavarz-Joud P / Finn MG | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Pleomorphism in wild-type and engineered PP7 virus-like particles Authors: Keshavarz-Joud P / Kopylov M / Zhao L / Hernandez C / Bobe D / Paraan M / Finn MG | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 483.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.2 KB 12.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 16.9 KB | Display | ![]() |
Images | ![]() | 115.2 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 475.5 MB 475.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 26.7 KB | Display | |
Data in CIF | ![]() | 34.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | sharpened b-factor: -66.5 | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.083 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: half a
File | emd_44782_half_map_1.map | ||||||||||||
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Annotation | half a | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half b
File | emd_44782_half_map_2.map | ||||||||||||
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Annotation | half b | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : D5E cage self-assembly of PP7-AY-PP7 coat protein dimer of PP7 ba...
Entire | Name: D5E cage self-assembly of PP7-AY-PP7 coat protein dimer of PP7 bacteriophage protein expressed in E.coli |
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Components |
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-Supramolecule #1: D5E cage self-assembly of PP7-AY-PP7 coat protein dimer of PP7 ba...
Supramolecule | Name: D5E cage self-assembly of PP7-AY-PP7 coat protein dimer of PP7 bacteriophage protein expressed in E.coli type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL |
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Buffer | pH: 7.5 / Component - Concentration: 0.1 M / Component - Name: PBS |
Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 52.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |