+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-43924 | |||||||||
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Title | Bovine adult muscle nAChR resting state | |||||||||
Map data | sharp | |||||||||
Sample |
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Keywords | Bovine muscle nicotinic acetylcholine receptor / MEMBRANE PROTEIN | |||||||||
Function / homology | Function and homology information Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Highly calcium permeable nicotinic acetylcholine receptors / postsynaptic membrane organization / skeletal muscle tissue growth / musculoskeletal movement / neuromuscular synaptic transmission / acetylcholine-gated channel complex / behavioral response to nicotine / muscle cell development ...Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / Highly calcium permeable nicotinic acetylcholine receptors / postsynaptic membrane organization / skeletal muscle tissue growth / musculoskeletal movement / neuromuscular synaptic transmission / acetylcholine-gated channel complex / behavioral response to nicotine / muscle cell development / acetylcholine receptor activity / synaptic transmission, cholinergic / acetylcholine binding / postsynaptic specialization membrane / acetylcholine receptor signaling pathway / nervous system process / channel activity / acetylcholine-gated monoatomic cation-selective channel activity / neuromuscular junction development / muscle cell cellular homeostasis / monoatomic cation transport / membrane depolarization / skeletal muscle contraction / neuronal action potential / muscle contraction / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / response to nicotine / neuromuscular junction / neuron cellular homeostasis / postsynaptic membrane / neuron projection / synapse / cell surface / signal transduction / plasma membrane Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) / synthetic construct (others) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.09 Å | |||||||||
Authors | Li H / Hibbs RE | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Nature / Year: 2024 Title: Structural switch in acetylcholine receptors in developing muscle Authors: Li H / Teng JF / Hibbs RE | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_43924.map.gz | 483.7 MB | EMDB map data format | |
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Header (meta data) | emd-43924-v30.xml emd-43924.xml | 23.2 KB 23.2 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_43924_fsc.xml | 16.8 KB | Display | FSC data file |
Images | emd_43924.png | 51.3 KB | ||
Filedesc metadata | emd-43924.cif.gz | 7.3 KB | ||
Others | emd_43924_additional_1.map.gz emd_43924_additional_2.map.gz emd_43924_half_map_1.map.gz emd_43924_half_map_2.map.gz | 258.5 MB 428.4 MB 474.5 MB 474.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-43924 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43924 | HTTPS FTP |
-Validation report
Summary document | emd_43924_validation.pdf.gz | 983.5 KB | Display | EMDB validaton report |
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Full document | emd_43924_full_validation.pdf.gz | 983.1 KB | Display | |
Data in XML | emd_43924_validation.xml.gz | 26.4 KB | Display | |
Data in CIF | emd_43924_validation.cif.gz | 34.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43924 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43924 | HTTPS FTP |
-Related structure data
Related structure data | 9avvMC 9avuC 9awjC 9awkC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_43924.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | sharp | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.935 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: unsharp
File | emd_43924_additional_1.map | ||||||||||||
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Annotation | unsharp | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: EMhancer
File | emd_43924_additional_2.map | ||||||||||||
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Annotation | EMhancer | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: adult resting halfmapA
File | emd_43924_half_map_1.map | ||||||||||||
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Annotation | adult_resting_halfmapA | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: adult resting halfmapB
File | emd_43924_half_map_2.map | ||||||||||||
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Annotation | adult_resting_halfmapB | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Bovine muscle nicotinic acetylcholine receptor
Entire | Name: Bovine muscle nicotinic acetylcholine receptor |
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Components |
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-Supramolecule #1: Bovine muscle nicotinic acetylcholine receptor
Supramolecule | Name: Bovine muscle nicotinic acetylcholine receptor / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: Bos taurus (cattle) |
-Macromolecule #1: Acetylcholine receptor subunit alpha
Macromolecule | Name: Acetylcholine receptor subunit alpha / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Bos taurus (cattle) |
Molecular weight | Theoretical: 49.949828 KDa |
Sequence | String: SEHETRLVAK LFEDYNSVVR PVEDHRQAVE VTVGLQLIQL INVDEVNQIV TTNVRLKQQW VDYNLKWNPD DYGGVKKIHI PSEKIWRPD LVLYNNADGD FAIVKFTKVL LDYTGHITWT PPAIFKSYCE IIVTHFPFDE QNCSMKLGTW TYDGSVVVIN P ESDQPDLS ...String: SEHETRLVAK LFEDYNSVVR PVEDHRQAVE VTVGLQLIQL INVDEVNQIV TTNVRLKQQW VDYNLKWNPD DYGGVKKIHI PSEKIWRPD LVLYNNADGD FAIVKFTKVL LDYTGHITWT PPAIFKSYCE IIVTHFPFDE QNCSMKLGTW TYDGSVVVIN P ESDQPDLS NFMESGEWVI KESRGWKHWV FYACCPSTPY LDITYHFVMQ RLPLYFIVNV IIPCLLFSFL TGLVFYLPTD SG EKMTLSI SVLLSLTVFL LVIVELIPST SSAVPLIGKY MLFTMVFVIA SIIITVIVIN THHRSPSTHV MPEWVRKVFI DTI PNIMFF STMKRPSREK QDKKIFTEDI DISDISGKPG PPPMGFHSPL IKHPEVKSAI EGIKYIAETM KSDQESNNAA EEWK YVAMV MDHILLAVFM LVCIIGTLAV FAGRLIELNQ QG UniProtKB: Acetylcholine receptor subunit alpha |
-Macromolecule #2: Acetylcholine receptor subunit beta
Macromolecule | Name: Acetylcholine receptor subunit beta / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Bos taurus (cattle) |
Molecular weight | Theoretical: 55.111832 KDa |
Sequence | String: SEAEGRLREK LFSGYDSTVR PAREVGDRVW VSIGLTLAQL ISLNEKDEEM STKVYLDLEW TDYRLSWDPE EHEGIDSLRI SAESVWLPD VVLLNNNDGN FDVALDINVV VSSDGSMRWQ PPGIYRSSCS IQVTYFPFDW QNCTMVFSSY SYDSSEVSLQ T GLSPEGQE ...String: SEAEGRLREK LFSGYDSTVR PAREVGDRVW VSIGLTLAQL ISLNEKDEEM STKVYLDLEW TDYRLSWDPE EHEGIDSLRI SAESVWLPD VVLLNNNDGN FDVALDINVV VSSDGSMRWQ PPGIYRSSCS IQVTYFPFDW QNCTMVFSSY SYDSSEVSLQ T GLSPEGQE RQEVYIHEGT FIENGQWEII HKPSRLIQPS VDPRGGGEGR REEVTFYLII RRKPLFYLVN VIAPCILITL LA IFVFYLP PDAGEKMGLS IFALLTLTVF LLLLADKVPE TSLSVPIIIK YLMFTMVLVT FSVILSVVVL NLHHRSPHTH QMP LWVRQI FIHKLPLYLG LKRPKPERDQ MQEPPSIAPR DSPGSGWGRG TDEYFIRKPP NDFLFPKPNR FQPELSAPDL RRFI DGPNR AVGLPPELRE VVSSISYIAR QLQEQEDHDV LKEDWQFVAM VVDRLFLWTF IIFTSVGTLV IFLDATYHLP PADPF P UniProtKB: Acetylcholine receptor subunit beta |
-Macromolecule #3: Acetylcholine receptor subunit delta
Macromolecule | Name: Acetylcholine receptor subunit delta / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Bos taurus (cattle) |
Molecular weight | Theoretical: 56.543926 KDa |
Sequence | String: LNEEERLIRH LFEEKAYNKE LRPAAHKESV EISLALTLSN LISLKEVEET LTTNVWIEQG WTDSRLQWDA EDFGNISVLR LPADMVWLP EIVLENNNDG SFQISYSCNV LIYPSGSVYW LPPAIFRSSC PISVTYFPFD WQNCSLKFSS LKYTTKEITL S LKQAEEDG ...String: LNEEERLIRH LFEEKAYNKE LRPAAHKESV EISLALTLSN LISLKEVEET LTTNVWIEQG WTDSRLQWDA EDFGNISVLR LPADMVWLP EIVLENNNDG SFQISYSCNV LIYPSGSVYW LPPAIFRSSC PISVTYFPFD WQNCSLKFSS LKYTTKEITL S LKQAEEDG RSYPVEWIII DPEGFTENGE WEIVHRPARV NVDPSVPLDS PNRQDVTFYL IIRRKPLFYV INILVPCVLI SF MINLVFY LPADCGEKTS MAISVLLAQS VFLLLISKRL PATSMAIPLI GKFLLFGMVL VTMVVVICVI VLNIHFRTPS THV LSEPVK KLFLETLPEI LHMSRPAEDG PSPGTLIRRS SSLGYISKAE EYFSLKSRSD LMFEKQSERH GLARRLTTAR RPPA GSEQA QQELFSELKP AVDGANFIVN HMKDQNNYNE EKDCWNRVAR TVDRLCLFVV TPIMVVGTAW IFLQGAYNQP PPQPF PGDP FSYLEKDKRF I UniProtKB: Acetylcholine receptor subunit delta |
-Macromolecule #4: Acetylcholine receptor subunit epsilon
Macromolecule | Name: Acetylcholine receptor subunit epsilon / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Bos taurus (cattle) |
Molecular weight | Theoretical: 52.613262 KDa |
Sequence | String: KNEELRLYHY LFDTYDPGRR PVQEPEDTVT ISLKVTLTNL ISLNEKEETL TTSVWIGIDW QDYRLNYSKG DFGGVETLRV PSELVWLPE IVLENNIDGQ FGVAYEANVL VSEGGYLSWL PPAIYRSTCA VEVTYFPFDW QNCSLVFRSQ TYNAEEVEFV F AVDDEGKT ...String: KNEELRLYHY LFDTYDPGRR PVQEPEDTVT ISLKVTLTNL ISLNEKEETL TTSVWIGIDW QDYRLNYSKG DFGGVETLRV PSELVWLPE IVLENNIDGQ FGVAYEANVL VSEGGYLSWL PPAIYRSTCA VEVTYFPFDW QNCSLVFRSQ TYNAEEVEFV F AVDDEGKT ISKIDIDTEA YTENGEWAID FCPGVIRRHD GDSAGGPGET DVIYSLIIRR KPLFYVINII VPCVLISGLV LL AYFLPAQ AGGQKCTVSI NVLLAQTVFL FLIAQKTPET SLSVPLLGRY LIFVMVVATL IVMNCVIVLN VSLRTPTTHA MSP RLRYVL LELLPQLLGS GAPPEIPRAA SPPRRASSLG LLLRAEELIL KKPRSELVFE QQRHRHGTWT ATLCQNLGAA APEI RCCVD AVNFVASSTR DQEATGEEVS DWVRMGKALD SICFWAALVL FLVGSSLIFL GAYFNRVPQL PYPPCM UniProtKB: Acetylcholine receptor subunit epsilon |
-Macromolecule #5: Toxin
Macromolecule | Name: Toxin / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: synthetic construct (others) |
Molecular weight | Theoretical: 6.993089 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: GSMICYNQQS SQPPTTKTCS ETSCYKKTWR DHRGTIIERG CGCPKVKPGI KLHCCRTDKC NN |
-Macromolecule #10: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(tri...
Macromolecule | Name: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate type: ligand / ID: 10 / Number of copies: 8 / Formula: POV |
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Molecular weight | Theoretical: 760.076 Da |
Chemical component information | ChemComp-POV: |
-Macromolecule #11: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 11 / Number of copies: 2 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ChemComp-NAG: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.6 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Initial model | Chain - Source name: Other / Chain - Initial model type: experimental model / Details: cryosparc ab initial model |
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Refinement | Space: REAL / Protocol: AB INITIO MODEL |
Output model | PDB-9avv: |