+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-4389 | |||||||||
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Title | P22 CellB loaded Wiffleball | |||||||||
Map data | P22 CelB loaded wiffleball | |||||||||
Sample |
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Biological species | Enterobacteria phage P22 (virus) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 12.3 Å | |||||||||
Authors | McCoy K / Selivanovitch E / Luque D / Lee B / Edwards E / Caston JR / Douglas T | |||||||||
Citation | Journal: Biomacromolecules / Year: 2018 Title: Cargo Retention inside P22 Virus-Like Particles. Authors: Kimberly McCoy / Ekaterina Selivanovitch / Daniel Luque / Byeongdu Lee / Ethan Edwards / José R Castón / Trevor Douglas / Abstract: Viral protein cages, with their regular and programmable architectures, are excellent platforms for the development of functional nanomaterials. The ability to transform a virus into a material with ...Viral protein cages, with their regular and programmable architectures, are excellent platforms for the development of functional nanomaterials. The ability to transform a virus into a material with intended structure and function relies on the existence of a well-understood model system, a noninfectious virus-like particle (VLP) counterpart. Here, we study the factors important to the ability of P22 VLP to retain or release various protein cargo molecules depending on the nature of the cargo, the capsid morphology, and the environmental conditions. Because the interaction between the internalized scaffold protein (SP) and the capsid coat protein (CP) is noncovalent, we have studied the efficiency with which a range of SP variants can dissociate from the interior of different P22 VLP morphologies and exit by traversing the porous capsid. Understanding the types of cargos that are either retained or released from the P22 VLP will aid in the rational design of functional nanomaterials. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_4389.map.gz | 20.7 MB | EMDB map data format | |
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Header (meta data) | emd-4389-v30.xml emd-4389.xml | 9 KB 9 KB | Display Display | EMDB header |
Images | emd_4389.png | 230.4 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-4389 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4389 | HTTPS FTP |
-Validation report
Summary document | emd_4389_validation.pdf.gz | 296 KB | Display | EMDB validaton report |
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Full document | emd_4389_full_validation.pdf.gz | 295.1 KB | Display | |
Data in XML | emd_4389_validation.xml.gz | 6.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4389 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4389 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_4389.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | P22 CelB loaded wiffleball | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Enterobacteria phage P22
Entire | Name: Enterobacteria phage P22 (virus) |
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Components |
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-Supramolecule #1: Enterobacteria phage P22
Supramolecule | Name: Enterobacteria phage P22 / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 10754 / Sci species name: Enterobacteria phage P22 / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: OTHER / Virus enveloped: No / Virus empty: Yes |
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Host (natural) | Organism: Salmonella enterica (bacteria) |
Host system | Organism: Escherichia coli (E. coli) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7 |
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Grid | Model: Quantifoil R2/2 / Material: COPPER/RHODIUM / Mesh: 300 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Image recording | Film or detector model: FEI EAGLE (4k x 4k) / Average electron dose: 20.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |