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基本情報
登録情報 | データベース: EMDB / ID: EMD-4349 | |||||||||||||||
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タイトル | Cryo-EM structure of a late human pre-40S ribosomal subunit - State A | |||||||||||||||
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![]() | 40S / pre-40S / ribosome biogenesis / RIBOSOME | |||||||||||||||
機能・相同性 | ![]() peptidyl-glutamine methylation / regulation of protein localization to nucleolus / rRNA (guanine-N7)-methylation / tRNA methyltransferase activator activity / rRNA (guanine) methyltransferase activity / tRNA modification in the nucleus and cytosol / Methylation / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / trophectodermal cell differentiation / protein methyltransferase activity ...peptidyl-glutamine methylation / regulation of protein localization to nucleolus / rRNA (guanine-N7)-methylation / tRNA methyltransferase activator activity / rRNA (guanine) methyltransferase activity / tRNA modification in the nucleus and cytosol / Methylation / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / trophectodermal cell differentiation / protein methyltransferase activity / tRNA methylation / positive regulation of rRNA processing / positive regulation of respiratory burst involved in inflammatory response / nucleolus organization / negative regulation of RNA splicing / rRNA methylation / neural crest cell differentiation / rRNA modification in the nucleus and cytosol / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / U3 snoRNA binding / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / Translation initiation complex formation / snoRNA binding / mammalian oogenesis stage / activation-induced cell death of T cells / fibroblast growth factor binding / Protein hydroxylation / monocyte chemotaxis / mTORC1-mediated signalling / SARS-CoV-1 modulates host translation machinery / Peptide chain elongation / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / negative regulation of respiratory burst involved in inflammatory response / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / TOR signaling / L13a-mediated translational silencing of Ceruloplasmin expression / T cell proliferation involved in immune response / Major pathway of rRNA processing in the nucleolus and cytosol / regulation of translational fidelity / erythrocyte development / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of ubiquitin-dependent protein catabolic process / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / translation regulator activity / Nuclear events stimulated by ALK signaling in cancer / ribosomal small subunit export from nucleus / positive regulation of cell cycle / stress granule assembly / Mitotic Prometaphase / cytosolic ribosome / EML4 and NUDC in mitotic spindle formation / rough endoplasmic reticulum / gastrulation / MDM2/MDM4 family protein binding / translation initiation factor binding / Resolution of Sister Chromatid Cohesion / transcription initiation-coupled chromatin remodeling / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / erythrocyte differentiation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / innate immune response in mucosa / stem cell proliferation / cellular response to leukemia inhibitory factor / methyltransferase activity / maturation of SSU-rRNA / neural tube closure / small-subunit processome / mRNA 3'-UTR binding / positive regulation of translation / translational initiation / RHO GTPases Activate Formins / liver regeneration / maintenance of translational fidelity / response to virus / placenta development / mRNA 5'-UTR binding / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / osteoblast differentiation / rRNA processing / G1/S transition of mitotic cell cycle / Separation of Sister Chromatids / antimicrobial humoral immune response mediated by antimicrobial peptide / ribosome biogenesis / apical part of cell / glucose homeostasis / chromatin organization 類似検索 - 分子機能 | |||||||||||||||
生物種 | ![]() | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.5 Å | |||||||||||||||
![]() | Ameismeier M / Cheng J | |||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Visualizing late states of human 40S ribosomal subunit maturation. 著者: Michael Ameismeier / Jingdong Cheng / Otto Berninghausen / Roland Beckmann / ![]() 要旨: The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high- ...The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage. | |||||||||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 15.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 45 KB 45 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 12.9 KB | 表示 | ![]() |
画像 | ![]() | 144.7 KB | ||
Filedesc metadata | ![]() | 10.3 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 6g4wMC ![]() 4337C ![]() 4348C ![]() 4350C ![]() 4351C ![]() 4352C ![]() 4353C ![]() 6g18C ![]() 6g4sC ![]() 6g51C ![]() 6g53C ![]() 6g5hC ![]() 6g5iC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.084 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
+全体 : Cryo-EM structure of a late human pre-40S ribosomal subunit - State A
+超分子 #1: Cryo-EM structure of a late human pre-40S ribosomal subunit - State A
+分子 #1: 18S ribosomal RNA
+分子 #2: 40S ribosomal protein S17
+分子 #3: 40S ribosomal protein S27
+分子 #4: 40S ribosomal protein S3a
+分子 #5: 40S ribosomal protein S28
+分子 #6: 40S ribosomal protein S4, X isoform
+分子 #7: 40S ribosomal protein S30
+分子 #8: 40S ribosomal protein S5
+分子 #9: 40S ribosomal protein S7
+分子 #10: 40S ribosomal protein S6
+分子 #11: 40S ribosomal protein S25
+分子 #12: 40S ribosomal protein S24
+分子 #13: RNA-binding protein PNO1
+分子 #14: 40S ribosomal protein S23
+分子 #15: Bystin
+分子 #16: 40S ribosomal protein S15a
+分子 #17: Pre-rRNA-processing protein TSR1 homolog
+分子 #18: UNKNOWN
+分子 #19: 40S ribosomal protein S19
+分子 #20: 40S ribosomal protein S18
+分子 #21: 40S ribosomal protein S16
+分子 #22: 40S ribosomal protein S15
+分子 #23: 40S ribosomal protein S14
+分子 #24: 40S ribosomal protein S13
+分子 #25: 40S ribosomal protein S11
+分子 #26: 40S ribosomal protein S9
+分子 #27: 40S ribosomal protein S8
+分子 #28: RRP12
+分子 #29: Multifunctional methyltransferase subunit TRM112-like protein
+分子 #30: Probable 18S rRNA (guanine-N(7))-methyltransferase
+分子 #31: UNKNOWN HELIX
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.6 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 2.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |