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- EMDB-4327: Structure of a partial yeast 48S preinitiation complex with eIF5 ... -

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Basic information

Entry
Database: EMDB / ID: EMD-4327
TitleStructure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (Map C1)
Map dataFor optimal visualization of eIF2 gamma and beta, gauss-filter the map by 1.1 and display it at 0.03 contour level
Sample
  • Complex: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
    • Complex: Ribosome
      • RNA: x 1 types
      • Protein or peptide: x 34 types
    • Complex: tRNA
      • RNA: x 1 types
    • Complex: Initiation factors
      • Protein or peptide: x 2 types
    • Complex: Initialtion factors
      • Protein or peptide: x 8 types
    • Complex: mRNA
      • RNA: x 1 types
  • Ligand: x 4 types
Keywordsribosome / translation / initiation factors / 40S / eIF1A / eIF3 / eIF2 / eIF5 / tRNAi / 48S PIC / small ribosome subunit
Function / homology
Function and homology information


formation of translation initiation ternary complex / eukaryotic initiation factor eIF2 binding / Recycling of eIF2:GDP / Cellular response to mitochondrial stress / eukaryotic translation initiation factor 3 complex, eIF3e / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / incipient cellular bud site / translation reinitiation ...formation of translation initiation ternary complex / eukaryotic initiation factor eIF2 binding / Recycling of eIF2:GDP / Cellular response to mitochondrial stress / eukaryotic translation initiation factor 3 complex, eIF3e / ABC-family proteins mediated transport / methionyl-initiator methionine tRNA binding / eukaryotic translation initiation factor 3 complex, eIF3m / incipient cellular bud site / translation reinitiation / eukaryotic translation initiation factor 2 complex / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / cytoplasmic translational initiation / multi-eIF complex / eukaryotic 43S preinitiation complex / formation of translation preinitiation complex / eukaryotic 48S preinitiation complex / positive regulation of translational fidelity / protein-synthesizing GTPase / regulation of translational initiation / GDP-dissociation inhibitor activity / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal small subunit binding / 90S preribosome / translation regulator activity / translation initiation factor binding / translation initiation factor activity / negative regulation of translational initiation / GTPase activator activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome assembly / maturation of SSU-rRNA / small-subunit processome / translational initiation / cytoplasmic stress granule / rRNA processing / double-stranded RNA binding / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / GTPase activity / mRNA binding / protein kinase binding / GTP binding / nucleolus / RNA binding / zinc ion binding / identical protein binding / nucleus / cytosol / cytoplasm
Similarity search - Function
EIF3I / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor eIF2A ...EIF3I / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit G, N-terminal / eIF3G, RNA recognition motif / Eukaryotic translation initiation factor 3 subunit G / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor eIF2A / : / eIF3a, PCI domain, TPR-like region / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / eIF4-gamma/eIF5/eIF2-epsilon / Domain at the C-termini of GCD6, eIF-2B epsilon, eIF-4 gamma and eIF-5 / W2 domain / W2 domain profile. / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / : / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / S1 domain profile. / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S5, eukaryotic/archaeal / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / S27a-like superfamily / : / Translation elongation factor EFTu-like, domain 2 / Ribosomal protein S7e signature. / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S30 / Ribosomal protein S17e, conserved site / Ribosomal protein S30 / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S25 / Ribosomal protein S27a / S25 ribosomal protein / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S27e signature. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S3, eukaryotic/archaeal / Elongation factor Tu domain 2
Similarity search - Domain/homology
Eukaryotic translation initiation factor 3 subunit G / KLLA0B11231p / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 2 subunit beta / Small ribosomal subunit protein eS32A / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Eukaryotic translation initiation factor 3 subunit C / Small ribosomal subunit protein eS28 ...Eukaryotic translation initiation factor 3 subunit G / KLLA0B11231p / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 2 subunit beta / Small ribosomal subunit protein eS32A / Eukaryotic translation initiation factor 2 subunit alpha / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit gamma / Eukaryotic translation initiation factor 3 subunit C / Small ribosomal subunit protein eS28 / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 5 / Eukaryotic translation initiation factor 1A / Eukaryotic translation initiation factor 3 subunit I / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein uS10 / KLLA0F18040p / Small ribosomal subunit protein uS5 / KLLA0F07843p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / RP21 / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein RACK1 / 40S ribosomal protein S27 / Small ribosomal subunit protein uS14 / KLLA0D10659p / Small ribosomal subunit protein uS3 / 40S ribosomal protein S26 / 40S ribosomal protein S7 / 40S ribosomal protein S24 / 40S ribosomal protein S30 / KLLA0B08173p / Small ribosomal subunit protein uS8 / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S4 / KLLA0B01562p / KLLA0B01474p / RP41 / S16a / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein uS9
Similarity search - Component
Biological speciesKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast) / Saccharomyces cerevisiae (brewer's yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsLlacer JL / Hussain T
Funding support United Kingdom, 3 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
Wellcome TrustWT096570 United Kingdom
FEBS United Kingdom
CitationJournal: Elife / Year: 2018
Title: Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.
Authors: Jose Luis Llácer / Tanweer Hussain / Adesh K Saini / Jagpreet Singh Nanda / Sukhvir Kaur / Yuliya Gordiyenko / Rakesh Kumar / Alan G Hinnebusch / Jon R Lorsch / V Ramakrishnan /
Abstract: In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA in a 'P' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) ...In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNA in a 'P' state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringent AUG selection, but the molecular basis of its dual function was unknown. We present a cryo-electron microscopy (cryo-EM) reconstruction of a yeast 48S pre-initiation complex (PIC), at an overall resolution of 3.0 Å, featuring the N-terminal domain (NTD) of eIF5 bound to the 40S subunit at the location vacated by eIF1. eIF5 interacts with and allows a more accommodated orientation of Met-tRNA. Substitutions of eIF5 residues involved in the eIF5-NTD/tRNA interaction influenced initiation at near-cognate UUG codons and the closed/open PIC conformation in vitro, consistent with direct stabilization of the codon:anticodon duplex by the wild-type eIF5-NTD. The present structure reveals the basis for a key role of eIF5 in start-codon selection.
History
Header (metadata) releaseAug 24, 2016-
DepositionMar 12, 2018-
Map releaseDec 5, 2018-
UpdateOct 1, 2025-
Current statusOct 1, 2025Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6fyx
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4327.png

Downloads & links

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Map

FileDownload / File: emd_4327.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFor optimal visualization of eIF2 gamma and beta, gauss-filter the map by 1.1 and display it at 0.03 contour level
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 300 pix.
= 402. Å		1.34 Å/pix.
x 300 pix.
= 402. Å		1.34 Å/pix.
x 300 pix.
= 402. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum-0.2522875 - 0.52949286
Average (Standard dev.)-0.000008797226 (±0.023695989)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 402.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.341.341.34
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z402.000402.000402.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ450450450
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.2520.529-0.000

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Supplemental data

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Half map: #1

Fileemd_4327_half_map_1.map
Projections & Slices
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Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_4327_half_map_2.map
Projections & Slices
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Sample components

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Entire : Structure of a partial yeast 48S preinitiation complex with eIF5 ...

EntireName: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
Components
  • Complex: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
    • Complex: Ribosome
      • RNA: 18S ribosomal RNA
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: 40S ribosomal protein S1
      • Protein or peptide: KLLA0F09812p
      • Protein or peptide: KLLA0D08305p
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: KLLA0D10659p
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: KLLA0E23673p
      • Protein or peptide: KLLA0B08173p
      • Protein or peptide: KLLA0A10483p
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: KLLA0F18040p
      • Protein or peptide: 40S ribosomal protein S14
      • Protein or peptide: KLLA0F07843p
      • Protein or peptide: 40S ribosomal protein S16
      • Protein or peptide: KLLA0B01474p
      • Protein or peptide: KLLA0B01562p
      • Protein or peptide: KLLA0A07194p
      • Protein or peptide: KLLA0F25542p
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: 40S ribosomal protein S22
      • Protein or peptide: KLLA0B11231p
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: KLLA0B06182p
      • Protein or peptide: 40S ribosomal protein S26
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S28
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
      • Protein or peptide: KLLA0E12277p
      • Protein or peptide: 60S ribosomal protein L41-A
    • Complex: tRNA
      • RNA: tRNAi
    • Complex: Initiation factors
      • Protein or peptide: Eukaryotic translation initiation factor 1A
      • Protein or peptide: Eukaryotic translation initiation factor 5
    • Complex: Initialtion factors
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit alpha
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit gamma
      • Protein or peptide: Eukaryotic translation initiation factor 2 subunit beta
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit B
      • Protein or peptide: eIF3c,eIF3c
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit G
      • Protein or peptide: Eukaryotic translation initiation factor 3 subunit I
    • Complex: mRNA
      • RNA: mRNA (31-MER)
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: METHIONINE
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

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Supramolecule #1: Structure of a partial yeast 48S preinitiation complex with eIF5 ...

SupramoleculeName: Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#47
Molecular weightTheoretical: 1.8 MDa

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #2, #4-#37
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)

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Supramolecule #3: tRNA

SupramoleculeName: tRNA / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Supramolecule #5: Initiation factors

SupramoleculeName: Initiation factors / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #38, #42
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #4: Initialtion factors

SupramoleculeName: Initialtion factors / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #39-#41, #43-#47
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Supramolecule #6: mRNA

SupramoleculeName: mRNA / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)

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Macromolecule #1: tRNAi

MacromoleculeName: tRNAi / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.713969 KDa
SequenceString:
AGCGCCGU(1MG)(2MG) CGCAG(H2U)GGAA GCGC(M2G)CAGGG CUCAU(T6A)ACCC UGAU(7MG)(H2U)(5MC) (5MC)U CGGAUCG(1MA)AA CCG(RIA)GCGGCG CUACCA

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Macromolecule #2: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 2
Details: rRNA modifications taken from: http://people.biochem.umass.edu/fournierlab/3dmodmap/ysssu2dframes43d.php
Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 579.454875 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA (PSU)UUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAA UU CUAGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA (PSU)UUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAA UU CUAGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGAC U CCUUGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAAC UUUCGAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAG(PSU) GACAAUA AA UAACGAUACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGG C AAGUCUGGUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUU GAACUUUGGG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACGGUUUUU CAACCGGAUC UUUCCUUCUG GCUAACCUGU ACUCCUUGU GGGUGCAGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUG(PSU)UCAAA GCAGGCGAAA GCUCGAA UA UAUUAGCAUG GAAUAAUGGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGA C GGUCGGGGGC AUCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGC CAAGGACGUU UUCAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGA(PSU) CAGAUAC(5MC)GU CGUAGUCUUA AC CAUAAAC UAUGCCGACU AGGGAUCGGG UGGUGUUUUU CUUAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGG GUUCUG GGGGGAGUAU GGUCGCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCU UAAUU UGAC(C4J)CAACA CGGGGAAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA U UUUGUGGG UGGUGGUGCA UGGCCGUUCU UAGU(PSU)GGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACC U UAACCUACUA AAUAGGGUUG CUGGCACUUG CCGGUUGACU CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUU UGAGGCAAUA ACA(2MG)GUCUGU GAUGCCCUUA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUA CAA CCUUGGCCGA GAGGUCUGGG UAAUCUUGUG AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAA C(2MG) AG(7MG)AAUUCCU AGUAAGCGCA AGUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCG (5MC)CC GUCGCUAGUA CCGAUUGAAU GGCUUAGUGA GGCCUCAGGA UUUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGA GCGAAG AAUCUGGUCA AACUUGGUCA UUUAGAGGAA CUAAAAGUCG UAACAAGGUU UCCGUAGGUG A(MA6)CCUGCGGA AGGAUCAUU A

GENBANK: GENBANK: CR382124.1

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Macromolecule #3: mRNA (31-MER)

MacromoleculeName: mRNA (31-MER) / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 15.344964 KDa
SequenceString:
GGGCUCUCUU CUCUCUCUAA CUAUAAAAAU GUCUCUUCUC UCUCUCGAU

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Macromolecule #4: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 28.264525 KDa
SequenceString: MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH ...String:
MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL PDRTQPWAIM PDLYFYRNPE EIEQQTAEEE AVASGEQTEE AVDATEEQTE AA EWAEEGQ AQEEEWN

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #5: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 29.013678 KDa
SequenceString: M(AYA)VGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGS EDH SFRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYA QS SHIRQIRKVI ...String:
M(AYA)VGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGS EDH SFRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYA QS SHIRQIRKVI SEILTREVQN STLAQLTSKL IPEVINKEIE NATKDIFPLQ NVHIRKVKLL KQPKFDLGSL LSLHGEAS A EEKGKKVAGF KDEILETV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #6: KLLA0F09812p

MacromoleculeName: KLLA0F09812p / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 27.649979 KDa
SequenceString: MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG ...String:
MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG SVSVRLIPAP RGSGIVASPA VKKLMQLAGV EDVYTSSTGS TRTLENTLKA AFVAIGNTYG FLTPNLWEVQ AL TPSPMDV YADYATASKK KL

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #7: KLLA0D08305p

MacromoleculeName: KLLA0D08305p / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 26.300535 KDa
SequenceString: MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ...String:
MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ETATRHVLLR QGVLGIKVKI MKDPSRNTSG PKALPDAVTI IEPKEEEPVL EPSVKDYRPT EPVEAAESA

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #8: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 29.617514 KDa
SequenceString: MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ...String:
MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ITDFIKFDTG KLVYVTGGRN LGRVGTIVHR ERHEGGFDLV HIKDSLENTF VTRLNNVFVI GEPGRPWISL PK GKGIKLT ISEERDRRRA QHGL

UniProtKB: 40S ribosomal protein S4

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Macromolecule #9: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 25.385975 KDa
SequenceString: MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR ...String:
MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR RVNQSIALLT IGAREAAFRN IKTIAETLAE ELINAAKGSS TSYAIKKKDE LERVAKSNR

UniProtKB: KLLA0D10659p

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Macromolecule #10: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 26.970391 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEVRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #11: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 21.735297 KDa
SequenceString:
MSDPQAKILS QAPTELELQV AQAFIDLENN SPELKADLRA LQFKSIREIE VAGGKKALAV FVPVPSLAAY HKVQIKLTRE LEKKFQDRH VIFLAERRIL PKPSRKSRQT QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKILLNSKDV Q HIDNKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: 40S ribosomal protein S7

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Macromolecule #12: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 22.642727 KDa
SequenceString: MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA ...String:
MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA AISSRPGQSG RCDGYILEGE ELAFYLRRLT AKK

UniProtKB: 40S ribosomal protein S8

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Macromolecule #13: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 21.587049 KDa
SequenceString:
MPRAPRTYSK TYSTPKRPYE SARLDAELKL AGEYGLKNKR EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRIGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI SQRHIAVGKQ IVNIPSFMVR LESEKHIDFA R TSPFGGAR PGRVARKRAA AAGGEEADEE

UniProtKB: RP21

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Macromolecule #14: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 12.584377 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQSGDQ RPQGKKY

UniProtKB: KLLA0B08173p

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Macromolecule #15: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 17.84393 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF TNPKAKANRK TKRWYKNVGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTRM HRTIVIRRD YLHYVPKYNR YEKRHKNVPA HVSPAFRVQV GDIVTVGQCR PISKTVRFNV LKVASATGKA NKQFAKF

UniProtKB: RP41

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Macromolecule #16: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 14.466398 KDa
SequenceString:
MSDVEEVQQV PVAELTIEDA LKVVLRTSLV HDGLARGLRE SAKALTRGEG QLAVLVESVT EEAISKLVQG LATENNVPLI KVADAKQLG EWAGLGKIDR DGNARKVVGA SVVVVKNWGA DTQEREILLE HFSQQ

UniProtKB: 40S ribosomal protein S12

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Macromolecule #17: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 16.989875 KDa
SequenceString:
MGRMHSKGKG MSSSAIPYSR NAPAWFKGSS DGVVEQIIKY ARKGLTPSQI GVLLRDAHGV TQAKVITGNK ILRILKSNGL APEIPEDLY FLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVSVLPPNW KYESATASAL VN

UniProtKB: KLLA0F18040p

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Macromolecule #18: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 14.530655 KDa
SequenceString:
MANVVQAKDN SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGT RSKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #19: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 15.986796 KDa
SequenceString:
MSEAAAPRKR SFKTYSYKGV DLEKLLEMPT EDFVKLAPAR VRRKFARGLS EKPAGLMKKL RAAKLSAPEN EKPAVVRTHL RNMIIVPEM IGSVVGVYNG KVFNQVEIRP EMVGHYLGEF SITYTPVRHG RAGATTSRFI PLR

UniProtKB: KLLA0F07843p

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Macromolecule #20: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 15.874531 KDa
SequenceString:
MSTVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVQ PEILRFKVYE PLLLVGLDKF ANIDIRVKVT GGGHVSQVYA IRQAIAKGL VAYHQKFVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGRGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #21: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 15.722216 KDa
SequenceString:
MGRVRTKTVK RASKALIEKY YPKLTMDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPDVS ALDLSHSNDV LNVDTQTAEL VNSLGLKLPL SVSSVSAVRD RRFRKRN

UniProtKB: KLLA0B01474p

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Macromolecule #22: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 17.084602 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNINVVY ALTTIRGVGR RYANLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ KDVNDGKDYH SLANNLESKL RDDLERLKKI RSHRGIRHFW GLRVRGQHTK TTGRRRA

UniProtKB: KLLA0B01562p

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Macromolecule #23: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 15.87901 KDa
SequenceString:
MPGVSVRDVP AQDFINNYAS FLQRQGKLEV PGYVDIVKTS AGNELPPQDS EGWFYKRAAS VARHIYLRKQ VGVGKLNKLY GGAKNRGVR PHKHVDASGS INRKVLQSLE KLGVVEISPK GGRRISDNGL RDLDRIAAAT LEDEE

UniProtKB: S16a

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Macromolecule #24: KLLA0F25542p

MacromoleculeName: KLLA0F25542p / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 13.337604 KDa
SequenceString:
MSQVEKKSEQ QQEVVIHKIR INLTSTKVKQ LENVSANIIK NAETFKLVKK GPVRLPTKVL KISTRKTPNG EGSKTWDTYE MRIHKRYID LEAPAHIVKR ITQITIEPGV DVEVIIAA

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #25: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21

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Macromolecule #26: 40S ribosomal protein S22

MacromoleculeName: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 14.645041 KDa
SequenceString:
MTRTSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIADVEKW TANLLPARQF GYVILTTSAG IMDHEEAHRK HVSGKILGFV Y

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #27: KLLA0B11231p

MacromoleculeName: KLLA0B11231p / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 16.047897 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAETTYK KRLLGTAFKS SPFGGSSHAK GIVLEKIGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: KLLA0B11231p

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Macromolecule #28: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 15.194549 KDa
SequenceString:
MSDAITIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEAYKA EKDAVSVFGF RTQYGGGKST GFGLVYNSVA DAKKFEPAY RLVRYGLAEK VEKASRQQRK QRKNRGKKIF GTGKSIAKKA ARRNAD

UniProtKB: 40S ribosomal protein S24

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Macromolecule #29: KLLA0B06182p

MacromoleculeName: KLLA0B06182p / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 12.002116 KDa
SequenceString:
MPPKQQLSKA AKAAAAMAGG KKSKKKWSKK SHKDKAKHAV VLDQDKFDRI MKEAPTYRYV SVSVLVDRFK LGGSLARVAL RHLENEGII KPVSKHSKQA IYTRATASE

UniProtKB: 40S ribosomal protein S25

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Macromolecule #30: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 13.539957 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSRSV PKDKAIKRMA IRNIVEAAAI RDLSEASVYA EYALPKTYNK LHYCISCAIH ARIVRVRSR TDRRIRAPPQ RPRFNRDNKV SPADAAKKAL

UniProtKB: 40S ribosomal protein S26

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Macromolecule #31: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 8.884362 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQSPRS HFLDVKCPGC LNITTVFSHA QTAVTCESCS TVLCTPTGGK AKLSEGTSFR RK

UniProtKB: 40S ribosomal protein S27

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Macromolecule #32: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 7.549824 KDa
SequenceString:
MDTKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTTRTI VRNVKGPVRE GDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28

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Macromolecule #33: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 6.66257 KDa
SequenceString:
MAHENVWYSH PRKFGKGSRQ CRISGSHSGL IRKYGLNIDR QSFREKANDI GFYKYR

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #34: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 7.141421 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEKQEKPKQP KGRAYKRLLY TRRFVNVTLT NGKRKMNPSP SSQ

UniProtKB: 40S ribosomal protein S30

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Macromolecule #35: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 17.110977 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIRHKHKKVK LAVLNYYKVD DEGKVAKLRK ECPNCGPGIF LANHGDRFYC GKCHSTFATQ K

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

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Macromolecule #36: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 35.830945 KDa
SequenceString: MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD ...String:
MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD DGRITFVSAG MDKIVRSWSL NEDSYRIEAD FIGHNNYINV VQPSPDGSLA ASAGKDGQIY VWNLKHKSAF MN FDAKDEV FALAFSPSRF WLTAATASGI KIYDLENEVL IDELKPEFAG YTKAQDPHAV SLAWSADGQT LFAGYTDNVI RVW QVMTAN

UniProtKB: Small ribosomal subunit protein RACK1

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Macromolecule #37: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast)
Molecular weightTheoretical: 3.354243 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

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Macromolecule #38: Eukaryotic translation initiation factor 1A

MacromoleculeName: Eukaryotic translation initiation factor 1A / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 17.462168 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString:
MGKKNTKGGK KGRRGKNDSD GPKRELIYKE EGQEYAQITK MLGNGRVEAS CFDGNKRMAH IRGKLRKKVW MGQGDIILVS LRDFQDDQC DVVHKYNLDE ARTLKNQGEL PENAKINETD NFGFESDEDV NFEFGNADED DEEGEDEELD IDDI

UniProtKB: Eukaryotic translation initiation factor 1A

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Macromolecule #39: Eukaryotic translation initiation factor 2 subunit alpha

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit alpha
type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 34.763652 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE ...String:
MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRR VSSEDIIKCE EKYQKSKTVH SILRYCAEKF QIPLEELYKT IAWPLSRKFG HAYEAFKLSI IDETVWEGIE P PSKDVLDE LKNYISKRLT PQAVKIRADV EVSCFSYEGI DAIKDALKSA EDMSTEQMQV KVKLVAAPLY VLTTQALDKQ KG IEQLESA IEKITEVITK YGGVCNITMP PKAVTATEDA ELQALLESKE LDNRSDSEDD EDESDDE

UniProtKB: Eukaryotic translation initiation factor 2 subunit alpha

+
Macromolecule #40: Eukaryotic translation initiation factor 2 subunit gamma

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit gamma
type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 57.942699 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK ...String:
MSDLQDQEPS IIINGNLEPV GEPDIVEETE VVAQETQETQ DADKPKKKVA FTGLEEDGET EEEKRKREFE EGGGLPEQPL NPDFSKLNP LSAEIINRQA TINIGTIGHV AHGKSTVVRA ISGVQTVRFK DELERNITIK LGYANAKIYK CQEPTCPEPD C YRSFKSDK EISPKCQRPG CPGRYKLVRH VSFVDCPGHD ILMSTMLSGA AVMDAALLLI AGNESCPQPQ TSEHLAAIEI MK LKHVIIL QNKVDLMREE SALEHQKSIL KFIRGTIADG APIVPISAQL KYNIDAVNEF IVKTIPVPPR DFMISPRLIV IRS FDVNKP GAEIEDLKGG VAGGSILNGV FKLGDEIEIR PGIVTKDDKG KIQCKPIFSN IVSLFAEQND LKFAVPGGLI GVGT KVDPT LCRADRLVGQ VVGAKGHLPN IYTDIEINYF LLRRLLGVKT DGQKQAKVRK LEPNEVLMVN IGSTATGARV VAVKA DMAR LQLTSPACTE INEKIALSRR IEKHWRLIGW ATIKKGTTLE PIA

UniProtKB: Eukaryotic translation initiation factor 2 subunit gamma

+
Macromolecule #41: Eukaryotic translation initiation factor 2 subunit beta

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit beta
type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 31.631309 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD ...String:
MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA DAEAEKEPTD DIAEALGELS LKKKKKKTK DSSVDAFEKE LAKAGLDNVD AESKEGTPSA NSSIQQEVGL PYSELLSRFF NILRTNNPEL AGDRSGPKFR I PPPVCLRD GKKTIFSNIQ DIAEKLHRSP EHLIQYLFAE LGTSGSVDGQ KRLVIKGKFQ SKQMENVLRR YILEYVTCKT CK SINTELK REQSNRLFFM VCKSCGSTRS VSSIKTGFQA TVGKRRRM

UniProtKB: Eukaryotic translation initiation factor 2 subunit beta

+
Macromolecule #42: Eukaryotic translation initiation factor 5

MacromoleculeName: Eukaryotic translation initiation factor 5 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 45.321977 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVL DGFINKFVLC GSCKNPETEI IITKDNDLVR DCKACGKRTP MDLRHKLSSF ILKNPPDSVS GSKKKKKAAT A SANVRGGG ...String:
MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVL DGFINKFVLC GSCKNPETEI IITKDNDLVR DCKACGKRTP MDLRHKLSSF ILKNPPDSVS GSKKKKKAAT A SANVRGGG LSISDIAQGK SQNAPSDGTG SSTPQHHDED EDELSRQIKA AASTLEDIEV KDDEWAVDMS EEAIRARAKE LE VNSELTQ LDEYGEWILE QAGEDKENLP SDVELYKKAA ELDVLNDPKI GCVLAQCLFD EDIVNEIAEH NAFFTKILVT PEY EKNFMG GIERFLGLEH KDLIPLLPKI LVQLYNNDII SEEEIMRFGT KSSKKFVPKE VSKKVRRAAK PFITWLETAE SDDD EEDDE

UniProtKB: Eukaryotic translation initiation factor 5

+
Macromolecule #43: Eukaryotic translation initiation factor 3 subunit A

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 110.517641 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV ELKKGKLLKD GLHQYKKLIQ GSTEGLVSV GAVARKFIDL VESKIASEQT RADELQKQEI DDDLEGGVTP ENLLISVYES DQSVAGFNDE AITSWLRFTW E SYRAVLDL ...String:
MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV ELKKGKLLKD GLHQYKKLIQ GSTEGLVSV GAVARKFIDL VESKIASEQT RADELQKQEI DDDLEGGVTP ENLLISVYES DQSVAGFNDE AITSWLRFTW E SYRAVLDL LRNNALLEIT YSGVVKKTMH FCLKYQRKNE FKRLAEMLRQ HLDAANYQQS KSGNNLVDLS DADTLQRYLD QR FQQVDVS VKLELWHEAY RSIEDVFHLM KISKRAPKPS TLANYYENLV KVFFVSGDPL LHTTAWKKFY KLYSTNPRAT EEE FKTYSS TIFLSAISTQ LDEIPSIGYD PHLRMYRLLN LDAKPTRKEM LQSIIEDESI YGKVDEELKE LYDIIEVNFD VDTV KQQLE NLLVKLSSKT YFSQYIAPLR DVIMRRVFVA ASQKFTTVSQ SELYKLATLP APLDLSAWDI EKSLLQAAVE DYVSI TIDH ESAKVTFAKD PFDIFASTAS KEVSEEENTE PEVQEEKEET DEALGPQETE DGEEKEEESD PVIIRNSYIH NKLLEL SNV LHDVDSFNNA SYMEKVRIAR ETLIKKNKDD LEKISKIVDE RVKRSQEQKQ KHMEHAALHA EQDAEVRQQR ILEEKAA IE AKLEEEAHRR LIEKKKREFE AIKEREITKM ITEVNAKGHV YIDPNEAKSL DLDTIKQVII AEVSKNKSEL ESRMEYAM K KLDHTERALR KVELPLLQKE VDKLQETDTA NYEAMKKKIV DAAKAEYEAR MADRKNLVMV YDDYLKFKEH VSGTKESEL AAIRNQKKAE LEAAKKARIE EVRKRRYEEA IARRKEEIAN AERQKRAQEL AEATRKQREI EEAAAKKSTP YSFRAGNREP PSTPSTLPK ATVSPDKAKL DMIAQKQREM EEAIEQRLAG RTAGGSSPAT PATPATPATP TPSSGPKKMT MAEKLRAKRL A KGGR

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

+
Macromolecule #44: Eukaryotic translation initiation factor 3 subunit B

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 88.241766 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPS AKVPVLKKAL TSLFSKAGKV VNMEFPIDEA TGKTKGFLFV ECGSMNDAKK IIKSFHGKRL DLKHRLFLYT M KDVERYNS ...String:
MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL EDIPVDDIDF SDLEEQYKVT EEFNFDQYIV VNGAPVIPS AKVPVLKKAL TSLFSKAGKV VNMEFPIDEA TGKTKGFLFV ECGSMNDAKK IIKSFHGKRL DLKHRLFLYT M KDVERYNS DDFDTEFREP DMPTFVPSSS LKSWLMDDKV RDQFVLQDDV KTSVFWNSMF NEEDSLVESR ENWSTNYVRF SP KGTYLFS YHQQGVTAWG GPNFDRLRRF YHPDVRNSSV SPNEKYLVTF STEPIIVEED NEFSPFTKKN EGHQLCIWDI ASG LLMATF PVIKSPYLKW PLVRWSYNDK YCARMVGDSL IVHDATKNFM PLEAKALKPS GIRDFSFAPE GVKLQPFRNG DEPS VLLAY WTPETNNSAC TATIAEVPRG RVLKTVNLVQ VSNVTLHWQN QAEFLCFNVE RHTKSGKTQF SNLQICRLTE RDIPV EKVE LKDSVFEFGW EPHGNRFVTI SVHEVADMNY AIPANTIRFY APETKEKTDV IKRWSLVKEI PKTFANTVSW SPAGRF VVV GALVGPNMRR SDLQFYDMDY PGEKNINDNN DVSASLKDVA HPTYSAATNI TWDPSGRYVT AWSSSLKHKV EHGYKIF NI AGNLVKEDII AGFKNFAWRP RPASILSNAE RKKVRKNLRE WSAQFEEQDA MEADTAMRDL ILHQRELLKQ WTEYREKI G QEMEKSMNFK IFDVQPEDAS DDFTTIEEIV EEVLEETKEK VE

UniProtKB: Eukaryotic translation initiation factor 3 subunit B

+
Macromolecule #45: eIF3c,eIF3c

MacromoleculeName: eIF3c,eIF3c / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 86.539539 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)QEDFF TRLQTIIDSR GKKTVNQQSL ISTLE ELLT VAEKPYEFIM AYLTLIPSRF DASANLSYQP IDQWKSSFND ISKLLSILDQ TIDTYQVNEF ADPIDFIEDE PKEDSD GVK RILGSIFSFV ERLDDEFMKS LLNIDPHSSD YLIRLRDEQS IYNLILRTQL YFEATLKDEH DLERALTRPF VKRLDHI YY KSENLIKIME TAAWNIIPAQ FKSKFTSKDQ LDSADYVDNL IDGLSTILSK QNNIAVQKRA ILYNIYYTAL NKDFQTAK D MLLTSQVQTN INQFDSSLQI LFNRVVVQLG LSAFKLCLIE ECHQILNDLL SSSHLREILG QQSLHRISLN SSNNASADE RARQCLPYHQ HINLDLIDVV FLTCSLLIEI PRMTAFYSGI KVKRIPYSPK SIRRSLEHYD KLSFQGPPET LRDYVLFAAK SMQKGNWRD SVKYLREIKS WALLPNMETV LNSLTERVQV ESLKTYFFSF KRFYSSFSVA KLAELFDLPE NKVVEVLQSV I AELEIPAK LNDEKTIFVV EKGDEITKLE EAMVKLNKEY KIAKERLNPP SNRR

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

+
Macromolecule #46: Eukaryotic translation initiation factor 3 subunit G

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit G / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 30.520502 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSEVAPEEII ENADGSRSII TYKIEDGVKY KITQKVKEVK VLEKVHKSVA ERKNWHKYGS EKGSPAGPSA VTARLGEEVE LRLSRNWKQ AEEERIQKEK ASLTKTGLQC RLCGNDHMTM NCPFKTILSE LSALEDPATS EGGVEAASEE KAGQVGGAGS I PGQYVPPS ...String:
MSEVAPEEII ENADGSRSII TYKIEDGVKY KITQKVKEVK VLEKVHKSVA ERKNWHKYGS EKGSPAGPSA VTARLGEEVE LRLSRNWKQ AEEERIQKEK ASLTKTGLQC RLCGNDHMTM NCPFKTILSE LSALEDPATS EGGVEAASEE KAGQVGGAGS I PGQYVPPS RRAGARDPSS DAYRDSRERD DMCTLKIMQV NENADENSLR EELLFPFAPI PRVSVVRNKE TGKSRGLAFV TF SSEEVAE QALRFLDGRG YMNLILRVEW SKPKVKE

UniProtKB: Eukaryotic translation initiation factor 3 subunit G

+
Macromolecule #47: Eukaryotic translation initiation factor 3 subunit I

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Molecular weightTheoretical: 38.803375 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI ...String:
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVAT WKSPVPVKRV EFSPCGNYFL AILDNVMKNP GSINIYEIER DSATHELTKV SEEPIHKIIT HEGLDAATVA G WSTKGKYI IAGHKDGKIS KYDVSNNYEY VDSIDLHEKS ISDMQFSPDL TYFITSSRDT NSFLVDVSTL QVLKKYETDC PL NTAVITP LKEFIILGGG QEAKDVTTTS ANEGKFEARF YHKIFEEEIG RVQGHFGPLN TVAISPQGTS YASGGEDGFI RLH HFEKSY FDFKYDVEKA AEAKEHMQEA N

UniProtKB: Eukaryotic translation initiation factor 3 subunit I

+
Macromolecule #48: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 48 / Number of copies: 117 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #49: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 49 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #50: METHIONINE

MacromoleculeName: METHIONINE / type: ligand / ID: 50 / Number of copies: 1 / Formula: MET
Molecular weightTheoretical: 149.211 Da
Chemical component information

ChemComp-MET:
METHIONINE

+
Macromolecule #51: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 51 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.15 mg/mL
BufferpH: 6.5
Component:
ConcentrationName
20.0 mMMES
5.0 mMMagnessium acetate
80.0 mMPotassium acetate
10.0 mMAmmonium acetate
2.0 mMDithiothreitol (DTT)
0.001 mMZinc acetate
0.6 mMATP
0.25 mMGDPCP
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK I

-
Electron microscopy

MicroscopeFEI POLARA 300
TemperatureMin: 90.0 K / Max: 100.0 K
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 3 / Number real images: 3600 / Average exposure time: 1.1 sec. / Average electron dose: 40.0 e/Å2
Details: Images were collected in movie-mode at 32 frames per second
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Calibrated magnification: 104478 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 78000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

+
Image processing

DetailsFEI Falcon III
Particle selectionNumber selected: 706713
CTF correctionSoftware - Name: Gctf / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v88

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 74772
Initial angle assignmentType: NOT APPLICABLE / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final 3D classificationSoftware - Name: RELION (ver. 1.4)

-
Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: OTHER / Overall B value: 66 / Target criteria: FSC
Output model

PDB-6fyx:
Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)

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