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- EMDB-4264: CryoEM Structure of INO80core -

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Basic information

Entry
Database: EMDB / ID: EMD-4264
TitleCryoEM Structure of INO80core
Map data
Sample
  • Complex: INO80core masked reconstruction
    • Protein or peptide: RuvB-like helicase
    • Protein or peptide: RuvB-like helicase
    • Protein or peptide: Ino80
    • Protein or peptide: les2
    • Protein or peptide: les6
    • Protein or peptide: Arp5
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
Function / homology
Function and homology information


DASH complex / protein transport along microtubule to mitotic spindle pole body / mitotic sister chromatid biorientation / attachment of spindle microtubules to kinetochore / Ino80 complex / attachment of mitotic spindle microtubules to kinetochore / ATP-dependent activity, acting on DNA / helicase activity / kinetochore / mitotic spindle ...DASH complex / protein transport along microtubule to mitotic spindle pole body / mitotic sister chromatid biorientation / attachment of spindle microtubules to kinetochore / Ino80 complex / attachment of mitotic spindle microtubules to kinetochore / ATP-dependent activity, acting on DNA / helicase activity / kinetochore / mitotic spindle / chromatin organization / DNA helicase / microtubule / chromatin remodeling / cell division / DNA repair / ATP hydrolysis activity / ATP binding / nucleus / cytoplasm
Similarity search - Function
DASH complex subunit Dad4 / DASH complex subunit Dad4 / INO80 complex subunit B-like conserved region / INO80 complex, subunit Ies2 / INO80 complex, subunit Ies6 / PAPA-1-like conserved region / PAPA-1 / Vps72/YL1, C-terminal / YL1 nuclear protein C-terminal domain / YL1 nuclear protein C-terminal domain ...DASH complex subunit Dad4 / DASH complex subunit Dad4 / INO80 complex subunit B-like conserved region / INO80 complex, subunit Ies2 / INO80 complex, subunit Ies6 / PAPA-1-like conserved region / PAPA-1 / Vps72/YL1, C-terminal / YL1 nuclear protein C-terminal domain / YL1 nuclear protein C-terminal domain / RuvB-like / RuvB-like, AAA-lid domain / RuvBL1/2, DNA/RNA binding domain / TIP49 P-loop domain / TIP49 AAA-lid domain / TIP49, P-loop domain / Actin / Actin family / Actin / ATPase, nucleotide binding domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
INO80 complex subunit B-like conserved region domain-containing protein / RuvB-like helicase / RuvB-like helicase / Uncharacterized protein / Vps72/YL1 C-terminal domain-containing protein
Similarity search - Component
Biological speciesChaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.754 Å
AuthorsEustermann S / Schall K / Kostrewa D / Strauss M / Hopfner K
Funding support Germany, 3 items
OrganizationGrant numberCountry
European Molecular Biology OrganizationEMBO ALTF 1098-2012 Germany
German Research FoundationCRC1064, GRK1721 Germany
European Research CouncilATMMACHINE Germany
CitationJournal: Nature / Year: 2018
Title: Structural basis for ATP-dependent chromatin remodelling by the INO80 complex.
Authors: Sebastian Eustermann / Kevin Schall / Dirk Kostrewa / Kristina Lakomek / Mike Strauss / Manuela Moldt / Karl-Peter Hopfner /
Abstract: In the eukaryotic nucleus, DNA is packaged in the form of nucleosomes, each of which comprises about 147 base pairs of DNA wrapped around a histone protein octamer. The position and histone ...In the eukaryotic nucleus, DNA is packaged in the form of nucleosomes, each of which comprises about 147 base pairs of DNA wrapped around a histone protein octamer. The position and histone composition of nucleosomes is governed by ATP-dependent chromatin remodellers such as the 15-subunit INO80 complex . INO80 regulates gene expression, DNA repair and replication by sliding nucleosomes, the exchange of histone H2A.Z with H2A, and the positioning of + 1 and -1 nucleosomes at promoter DNA. The structures and mechanisms of these remodelling reactions are currently unknown. Here we report the cryo-electron microscopy structure of the evolutionarily conserved core of the INO80 complex from the fungus Chaetomium thermophilum bound to a nucleosome, at a global resolution of 4.3 Å and with major parts at 3.7 Å. The INO80 core cradles one entire gyre of the nucleosome through multivalent DNA and histone contacts. An Rvb1/Rvb2 AAA ATPase heterohexamer is an assembly scaffold for the complex and acts as a 'stator' for the motor and nucleosome-gripping subunits. The Swi2/Snf2 ATPase motor binds to nucleosomal DNA at superhelical location -6, unwraps approximately 15 base pairs, disrupts the H2A-DNA contacts and is poised to pump entry DNA into the nucleosome. Arp5 and Ies6 bind superhelical locations -2 and -3 to act as a counter grip for the motor, on the other side of the H2A-H2B dimer. The Arp5 insertion domain forms a grappler element that binds the nucleosome dyad, connects the Arp5 actin-fold and entry DNA over a distance of about 90 Å and packs against histone H2A-H2B near the 'acidic patch'. Our structure together with biochemical data suggests a unified mechanism for nucleosome sliding and histone editing by INO80. The motor is part of a macromolecular ratchet, persistently pumping entry DNA across the H2A-H2B dimer against the Arp5 grip until a large nucleosome translocation step occurs. The transient exposure of H2A-H2B by motor activity as well as differential recognition of H2A.Z and H2A may regulate histone exchange.
History
DepositionJan 15, 2018-
Header (metadata) releaseApr 25, 2018-
Map releaseApr 25, 2018-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.04
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  • Surface view with fitted model
  • Atomic models: PDB-6fhs
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_4264.map.gz / Format: CCP4 / Size: 149.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum-0.119740315 - 0.19093303
Average (Standard dev.)0.00004564429 (±0.00499873)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions340340340
Spacing340340340
CellA=B=C: 360.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z340340340
origin x/y/z0.0000.0000.000
length x/y/z360.400360.400360.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS340340340
D min/max/mean-0.1200.1910.000

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Supplemental data

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Sample components

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Entire : INO80core masked reconstruction

EntireName: INO80core masked reconstruction
Components
  • Complex: INO80core masked reconstruction
    • Protein or peptide: RuvB-like helicase
    • Protein or peptide: RuvB-like helicase
    • Protein or peptide: Ino80
    • Protein or peptide: les2
    • Protein or peptide: les6
    • Protein or peptide: Arp5
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: INO80core masked reconstruction

SupramoleculeName: INO80core masked reconstruction / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#6
Details: 3xRvb1, 3x Rvb2, Ino80insert, Arp5, Ies2, Ies6 other parts of complex are masked out
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
Molecular weightTheoretical: 600 KDa

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Macromolecule #1: RuvB-like helicase

MacromoleculeName: RuvB-like helicase / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 50.451848 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MVQISEVRGN TRDHRTAAHT HIKGLGLNSS GIAEKQAAGF VGQCAAREAC GVVVDLIKAH KMAGRGVLLA GGPGTGKTAL ALAISQELG TKIPFCPITG SEIYSTEVKK TEVLMENFRR AIGLRVRETK DVYEGEVTEM TPEEAENPLG GYGKTISTLL I GLKSARGQ ...String:
MVQISEVRGN TRDHRTAAHT HIKGLGLNSS GIAEKQAAGF VGQCAAREAC GVVVDLIKAH KMAGRGVLLA GGPGTGKTAL ALAISQELG TKIPFCPITG SEIYSTEVKK TEVLMENFRR AIGLRVRETK DVYEGEVTEM TPEEAENPLG GYGKTISTLL I GLKSARGQ KKLRLDPSIY EAIQKERVQV GDVIYIETNT GACKRVGRSD AYATEFDLEA EEYVPIPKGE VHKKKEIVQD VT LHDLDVA NARPQGGQDI ISMMGQLMKP KMTEITDKLR MEINKVVQKY INQGVAELIP GVLFIDEAHM LDIECFTYLN KAL ESPIAP IVVLASNRGI ATIRGADDLK AAHGIPPDFL QRLLIIPTHP YEPDEIRRIV RIRAQTEGVQ LTDAAVDRVA EHGV RISLR YCLQLLAPAS ILARVNGRTQ VDVQDIAEAE ELFLDARRSA NILTSTGESG GLHGFIS

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Macromolecule #2: RuvB-like helicase

MacromoleculeName: RuvB-like helicase / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 53.212746 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAAPLVTSVT ETKELRGLNL IAAHSHIRGL GVDADTLEPR PSSQGLVGQE KARKAAAVVL EMIKQGKIAG RAVLIAGPPS TGKTAIAMG MAQSLGQDVP FTTLAASEIF SLEMSKTEAL TQAFRKSIGV RIKEESEIME GEVVEIQIDR SVTGGAKQGK L TIKTTDME ...String:
MAAPLVTSVT ETKELRGLNL IAAHSHIRGL GVDADTLEPR PSSQGLVGQE KARKAAAVVL EMIKQGKIAG RAVLIAGPPS TGKTAIAMG MAQSLGQDVP FTTLAASEIF SLEMSKTEAL TQAFRKSIGV RIKEESEIME GEVVEIQIDR SVTGGAKQGK L TIKTTDME AIYDMGSKMI DAMTKERVMA GDIISIDKSS GKITKLGRSY ARSRDYDAMG VDTKFLQCPE GELQKRKEVV HT VSLHEID VINSRTQGFL ALFSGDTGEI RSEIRDQINT KVAEWKEEGK AEIVPGVLFI DEVHMLDIEC FSYINRALES DLA PIVIMA SNRGVSRIRG TDYKSPHGLP LDFLDRVVII NTHPYTPDEL RQILSIRAQE EEVDLTPDAL ALLTKIGQEA GLRY ASNLI TTSQLIAAKR RAKQVGVEDV QRSFKLFYDP ARSVRFVQES EKRLIGNDGV VDFSYQGAAE AAAPTLPAAA PVDPV GGEK MDMS

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Macromolecule #3: Ino80

MacromoleculeName: Ino80 / type: protein_or_peptide / ID: 3 / Details: C-terminal double FlagTag / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Molecular weightTheoretical: 127.529781 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MTDSYATKAS NLKKTAILAS KEAKRWQLRT NKGTKDLQAR AKRVMRDMMG FWKRNEREER DLRKAAERLE LENARKEEAD REAARQRRK LNFLISQTEL YSHFISKKIK THEVERSTDH PDVATDEKDK IPEPTLNINV PEPTGPIAPK VTDFNSLDFD N EDESALQA ...String:
MTDSYATKAS NLKKTAILAS KEAKRWQLRT NKGTKDLQAR AKRVMRDMMG FWKRNEREER DLRKAAERLE LENARKEEAD REAARQRRK LNFLISQTEL YSHFISKKIK THEVERSTDH PDVATDEKDK IPEPTLNINV PEPTGPIAPK VTDFNSLDFD N EDESALQA AAMANAQNAI AEAQKKAREF NKDETKLDED GEMNFQHPEL TEFEVAQPKL LNCQLKEYQL KGLNWLVNLY EQ GINGILA DEMGLGKTVQ SISVMAYLAE RYDIWGPFLV VAPASTLHNW QQEVSKFVPD FKVLPYWGTA ADRKVLRKFW DRK HTTYKK DSPFHVMITS YQLVVSDVAY FQKMKWQYMI LDEAQAIKSS QSSRWKCLLG FHCRNRLLLT GTPIQNNMQE LWAL LHFIM PSLFDSHDEF SEWFSKDIES HAQSNTKLNE DQLKRLHMIL KPFMLRRVKK HVQKELGDKI EIDVFCELSY RQRAM YQSL RNQISIMDLI EKATVGDNED SATLMNLVMQ FRKVCNHPDL FERADTSSPF FCGHFAETGS FLREGTNVAL GYSTRS LVE YRLPRLIWCD GGRLDKPGPG NLVAGFRSKY LNHMMNIWTP ENIRSSLEGI ENFTWLRFVD TSLQEAYRAS HTDVFAR AV DLASKQNRLG HMQIVYDEPE DKKWTPVHAL FQICERENPK AVAEITTEGV LRDLMNIARV KYRELGLCRL EKAARPRA S APPIEVVCDS RSAVIERENI MFHPAMRKAL FGPTPSEIKE ASFGPRPVTL YPPRALLPAP DHDKQRFTNI TVPSMARFV TDSGKLAKLD ELLRELKEGG HRVLLYFQMT RMIDLMEEYL TYRNYKYCRL DGSTKLEDRR DTVADFQTRP EIFIFLLSTR AGGLGINLT TADTVIFYDS DWNPTIDSQA MDRAHRLGQT KQVTVYRLIT RGTIEERIRK RALQKEEVQR VVITGTGSVD F SGRRPPEN RNRDIAMWLA DDEQAEMIER REKELIESGE YDKIMQQRRK GGKRKRGAAN GDTVPSLEDM YHEGEGHFDD NK GSGAATP VDADSLGRGG KRKKAGGSKK AKTTKQRLAI ADGEIDDGEI DIDYKDDDDK GTDYKDDDDK

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Macromolecule #4: les2

MacromoleculeName: les2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 53.258902 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSTRPRRHAA QRASQAITDL ADRDRESDHS HGPISSRMSS FNSSSRSRLP GKGIASVSRS EAGGASDPEH IHLTVKLPSS KLRQATSSS GIKKAGSVGS SSSSSGGGKA AVKRARGGKR SRVLESSEEE EEENEVEVLG DEDEEEEEEE DEIEVREGEG Y DEDEEDVE ...String:
MSTRPRRHAA QRASQAITDL ADRDRESDHS HGPISSRMSS FNSSSRSRLP GKGIASVSRS EAGGASDPEH IHLTVKLPSS KLRQATSSS GIKKAGSVGS SSSSSGGGKA AVKRARGGKR SRVLESSEEE EEENEVEVLG DEDEEEEEEE DEIEVREGEG Y DEDEEDVE DEDEEMQDLG EEDADGEDDE MDVDAEGEED ADGDVNMDAG VVGARATTVR AVPPAIKVTK PPKESPSNGK AA TASKAND NAVPVKRPAP DSDDESLSSL ESEPEEEVNV AGGEDAEGED DDAEGEVDAE GEEEEEEEEI EVADEDAEGE DVE QDEDED EEEEDDDDEM ISRAQTPDMS RLTARQRARL GEASGEYLKL SDEVQSKKHF TAEELSMRRA EMARRRRNLS EKRN EEIKM ETVNKLLKKQ APRTTRRAAQ AAAAAEEAEE AAKQPKRPDP MMIRWVNNKM GSVVAVPEEL LGTHAGVVFG AGPGK GLPA GKMVEEV

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Macromolecule #5: les6

MacromoleculeName: les6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 23.127523 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MSNPDAQSAQ AAHQALVEQL DLHSIHKTFR NPNWRPNQRR NKTIKAILGE SQRKEASSTS AVATPRADDN GGGSGADTPA NNDNNDGLS TSGTSTPANG NGSGAGTPAS NGQPNLAQAS RSLQKLVLEK SLASAQAPDK KAANGFASSA PTATYTNIES A PSLAPMKH ...String:
MSNPDAQSAQ AAHQALVEQL DLHSIHKTFR NPNWRPNQRR NKTIKAILGE SQRKEASSTS AVATPRADDN GGGSGADTPA NNDNNDGLS TSGTSTPANG NGSGAGTPAS NGQPNLAQAS RSLQKLVLEK SLASAQAPDK KAANGFASSA PTATYTNIES A PSLAPMKH YCDVTGLPAP YLDPKTRLRY HNKEIFAMIR NLPQGMGEQF LEARGAHTVL K

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Macromolecule #6: Arp5

MacromoleculeName: Arp5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum var. thermophilum DSM 1495 (fungus)
Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 87.773086 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAPSAVAEPP PIPQRDEPWK RLPPPTVYPV KEARFEKYIP PQLDGRERAL AQPPGQVAIV IDNGSHSVRA GWNFEDKPRL AIPPIMSKY RDRKMGKTFS FAGSDCYADT TARSHIRNAF EAGTGIVSNW DVMEHVLDYV FVKLGMNECD GAIDMPIVMT E AVANLPYS ...String:
MAPSAVAEPP PIPQRDEPWK RLPPPTVYPV KEARFEKYIP PQLDGRERAL AQPPGQVAIV IDNGSHSVRA GWNFEDKPRL AIPPIMSKY RDRKMGKTFS FAGSDCYADT TARSHIRNAF EAGTGIVSNW DVMEHVLDYV FVKLGMNECD GAIDMPIVMT E AVANLPYS RKSMSEIIFE CYGAPSLVYG IDSLFSFRHN QGQTGLVVSS SYSATHVIPV YNRKALLSQA IRLNWGGWHM AE YMLKLLK LKYYTGFPGK LNSSQTEHMV RDFCYVSLDY DRELAGYLDW TGLEDRERIV QYPYTEEVVV QKTEEELARI AER KKESGR RLQEQAAKMR LERLMKKEQE LEYYKDIQRR MQGESKKEIK RLLDEAELKD EAALERVIRD LERSIKRARQ KDLG EPEEE EVPDFSLLDV PDDQLDEAGL RQKRQQRLLK SNWEARQRAK AEKEAEKARL AEEARLDEER RKNDLEGWLE EKRQL RLAK LNQLKERERL KADLGNRKSL ASQIRMKNIA NLASDNPTGS GSRKRRRGGA GADQDDDFGA DDADWGVYRS VAIGAN KGD DSDDEEGEED LEAAIRSLEN DLLRYDKTFS YDMTLDAQRD WSKSLLHAFR YGPRPFDPSS QAETHRVHLN VERIRVP EV LFQPAAIAGV DQAGLVEIAG DILCQRLPSL PGIQDAPDAF LRDVFLTGGN TLFQNFDERL RQGLMALLPV GAPLRVRR A QDAILDAWRG AAGWACTEEA KAAWITREEY LEKGGEYIKE HDLGNAFA

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Macromolecule #7: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 7 / Number of copies: 6 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Macromolecule #8: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 8 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 8
Details: 20 mM HEPES pH 8, 60 mM KCl, 0.5% glycerol, 0.25 mM CaCl2, 20 uM ZnCl2, 0.25 mM DTT, 0.05% Octyl-beta-glucoside
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 281 K / Instrument: LEICA EM GP
DetailsMonodisperse sample: INO80core complex reconstituted with nucleosomal substrate was purified by gelfiltration. Addition of nucleotides or crosslinking was not required.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus min: 1.3 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Number real images: 3992 / Average electron dose: 59.6 e/Å2
Details: Images were collected in movie mode with 4 frames per second and 10s total aquisition
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 251692
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: OTHER
Details: An initial ab initio model was generated using CryoSPARC
Initial angle assignmentType: RANDOM ASSIGNMENT / Software - Name: cryoSPARC
Details: An initial ab initio model was generated using CryoSPARC
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.1.1b)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.754 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1.1b) / Number images used: 144278
FSC plot (resolution estimation)

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