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- EMDB-42217: Mus musculus Otopetrin 2 (mOTOP2) in pH 7.0, intermediate state -
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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Mus musculus Otopetrin 2 (mOTOP2) in pH 7.0, intermediate state | ||||||||||||
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![]() | Proton channel / Otop2 / Otopetrin / Mus musculus / MEMBRANE PROTEIN | ||||||||||||
Function / homology | Otopetrin / Otopetrin / proton channel activity / proton transmembrane transport / membrane / plasma membrane / Proton channel OTOP2![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.79 Å | ||||||||||||
![]() | Gan N / Jiang Y | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural mechanism of proton conduction in otopetrin proton channel Authors: Gan N / Jiang Y | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 80.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.9 KB 12.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.3 KB | Display | ![]() |
Images | ![]() | 31.8 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Others | ![]() ![]() | 69.9 MB 69.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ug8 M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_42217_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_42217_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : mOTOP2
Entire | Name: mOTOP2 |
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Components |
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-Supramolecule #1: mOTOP2
Supramolecule | Name: mOTOP2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Proton channel OTOP2
Macromolecule | Name: Proton channel OTOP2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 62.643996 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MSEELVPHPN ESLPGPRASP REVWKKGGRL LSVLLAVNVL LLACTLISGG AFNKVAVYDT DVFALLTTMM LLAALWIVFY LLRTARCPD AVPYRDAHAG PIWLRGGLVL FGICTLVMDV FKTGYYSSFF ECQSAIKILH PIIQAVFVIV QTYFLWISAK D CIHTHLDL ...String: MSEELVPHPN ESLPGPRASP REVWKKGGRL LSVLLAVNVL LLACTLISGG AFNKVAVYDT DVFALLTTMM LLAALWIVFY LLRTARCPD AVPYRDAHAG PIWLRGGLVL FGICTLVMDV FKTGYYSSFF ECQSAIKILH PIIQAVFVIV QTYFLWISAK D CIHTHLDL TRCGLMFTLA TNLAIWMAAV VDESVHQAHS YSGSHGNTSH TRLNPDSKRA GGAAEEDPCL CSTAICQIFQ QG YFYLYPF NIEYSLFAST MLYVMWKNVG RLLASTHGHG HTPSRVSLFR ETFFAGPVLG LLLFVVGLAV FILYEVQVSG ERG HTRQAL VIYYSFNIVC LGLMTLVSLS GSVIYRFDRR AMDHHKNPTR TLDVALLMGA ALGQYAISYY SIVAVVVGSP RDLQ GALNL SHALLMIAQH TFQNVFIIES LHRGPPGAEP REMPPKEPCQ GITFANLDAI RTLPSCPPTP RLVIPNLESP QEAVA IISA PRCHWRRRCL KDISLFLLLC NVILWIMPAF GARPHFSNTV EVDFYGYSLW AAIVNICLPF GIFYRMHAVS SLLEVY VLS UniProtKB: Proton channel OTOP2 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: DIFFRACTION / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.9 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |