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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Caenorhabditis elegans Otopetrin 8 (CeOtop8) in pH 8.0 | ||||||||||||
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![]() | Proton channel / Otop / C.elegans / Otopetrin / MEMBRANE PROTEIN | ||||||||||||
Function / homology | Otopetrin / Otopetrin / proton channel activity / proton transmembrane transport / membrane / plasma membrane / Otopetrin-2![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.02 Å | ||||||||||||
![]() | Gan N / Jiang Y | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insight into the proton-conducting pathway of otopetrin proton channel Authors: Gan N / Jiang Y | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 75.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.9 KB 12.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.2 KB | Display | ![]() |
Images | ![]() | 33.5 KB | ||
Filedesc metadata | ![]() | 5.2 KB | ||
Others | ![]() ![]() | 69.8 MB 69.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ug4 M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_42213_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_42213_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : CeOTOP8
Entire | Name: CeOTOP8 |
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Components |
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-Supramolecule #1: CeOTOP8
Supramolecule | Name: CeOTOP8 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Otopetrin-2
Macromolecule | Name: Otopetrin-2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 69.791508 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MLMVDEQLKI GATPMYSPSH NPIVIADDME KGGSDSDSLP SSPTHHHELY RRPWIHEPRA TNFFVRLITS LYALILTIIS LVVEVSPTW RTDMWLAETI FYISMYGVGI LFFAYCYIFI IYPGPYNQLI SVLRKYKIIK NSEVWFIMQS QHNGEGAGTL Y LRLGALFF ...String: MLMVDEQLKI GATPMYSPSH NPIVIADDME KGGSDSDSLP SSPTHHHELY RRPWIHEPRA TNFFVRLITS LYALILTIIS LVVEVSPTW RTDMWLAETI FYISMYGVGI LFFAYCYIFI IYPGPYNQLI SVLRKYKIIK NSEVWFIMQS QHNGEGAGTL Y LRLGALFF GSVGIVLFGL ELFLCIENVA CKKVAIAKMI VAIVFTFIQM HFIFCNSKIT VNSSRKIVAF GMMHLISVNL WT WFRFVLA KQEAKAHKKA QLKQTFRKYY SSSSSSSSEE IHELISAVLN STLNNTPATK TMEPVASRLF ALEHFGDVAT FLT TCIVEY SLIGAAIMFI LWKSIGQNNH QQSNSGKRKV KMRIDCSSSS TGLFAGIIFL IGSLVSMGMY TIFESLRNSS GAQL VFGIV DLSLFSIALG ACIIGLWRMR HLQYRLHAHG EVIDEILLII GLIGEILYCA VGIDVFITCR RSADLTVSAL PAFVF VIRM IQVVVQAAFI LTTSRLRCLS KYSMKYKPGK EIITFLLVSN VTLFVFHTFE GMKSSFGFSS KAATQYNYII YAVGPL LVF YRFHSSACLA EIWKHTYSTK SNEYDHEHMS LSDSNLTAIT PISDIKEIPS PQLLKH UniProtKB: Otopetrin-2 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: DIFFRACTION / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.9 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |