+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-41263 | |||||||||
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Title | Purified alpha-CaMKII Holoenzymes | |||||||||
Map data | ||||||||||
Sample |
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Keywords | CaMKII / calmodulin / tomography / segmentation / SIGNALING PROTEIN | |||||||||
Biological species | Rattus norvegicus (Norway rat) | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Swulius MT | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To be Published Title: Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models. Authors: Purnell C / Heebner J / Hylton RH / Kabonick S / Grillo M / Grigoryev S / Heberle F / Waxham NM / Swulius MT | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_41263.map.gz | 66 MB | EMDB map data format | |
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Header (meta data) | emd-41263-v30.xml emd-41263.xml | 9.6 KB 9.6 KB | Display Display | EMDB header |
Images | emd_41263.png | 225.9 KB | ||
Filedesc metadata | emd-41263.cif.gz | 3.4 KB | ||
Others | emd_41263_additional_1.map.gz | 785.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-41263 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-41263 | HTTPS FTP |
-Validation report
Summary document | emd_41263_validation.pdf.gz | 439.5 KB | Display | EMDB validaton report |
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Full document | emd_41263_full_validation.pdf.gz | 439.1 KB | Display | |
Data in XML | emd_41263_validation.xml.gz | 2.3 KB | Display | |
Data in CIF | emd_41263_validation.cif.gz | 2.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41263 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41263 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_41263.map.gz / Format: CCP4 / Size: 120.8 MB / Type: IMAGE STORED AS SIGNED BYTE | ||||||||||||||||||||
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Voxel size | X=Y=Z: 5.4 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: #1
File | emd_41263_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Purified alpha-CaMKII Holoenzyme
Entire | Name: Purified alpha-CaMKII Holoenzyme |
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Components |
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-Supramolecule #1: Purified alpha-CaMKII Holoenzyme
Supramolecule | Name: Purified alpha-CaMKII Holoenzyme / type: complex / ID: 1 / Parent: 0 Details: Weighted Back-projection and Greyscale Segmentation |
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Source (natural) | Organism: Rattus norvegicus (Norway rat) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 6.8 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
-Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 5.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 60 |
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