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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryotomogram of DIV 4 cultured hippocampal neuron | |||||||||
![]() | DIV 4 Hippocampal Neuron (Weighted Backprojection) | |||||||||
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![]() | Neuron / Tomogram / denoise / segmentation / CELL ADHESION | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Swulius MT | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models. Authors: Purnell C / Heebner J / Hylton RH / Kabonick S / Grillo M / Grigoryev S / Heberle F / Waxham NM / Swulius MT | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 39 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.8 KB 11.8 KB | Display Display | ![]() |
Images | ![]() | 193.8 KB | ||
Filedesc metadata | ![]() | 3.4 KB | ||
Others | ![]() ![]() | 53.8 MB 688.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | DIV 4 Hippocampal Neuron (Weighted Backprojection) | ||||||||||||||||||||
Voxel size | X=Y=Z: 13.6 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: DIV 4 Hippocampal Neuron (regression denoised)
File | emd_41256_additional_1.map | ||||||||||||
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Annotation | DIV 4 Hippocampal Neuron (regression denoised) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: DIV 4 Hippocampal Neuron (Greyscale Segmentation)
File | emd_41256_additional_2.map | ||||||||||||
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Annotation | DIV 4 Hippocampal Neuron (Greyscale Segmentation) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : DIV 4 Hippocampal Neuron
Entire | Name: DIV 4 Hippocampal Neuron |
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Components |
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-Supramolecule #1: DIV 4 Hippocampal Neuron
Supramolecule | Name: DIV 4 Hippocampal Neuron / type: cell / ID: 1 / Parent: 0 Details: Weighted Backprojection, Regression Denoised, and Greyscale Segmentation |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 2.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 8.0 µm / Nominal defocus min: 8.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Number images used: 60 |
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