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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Purified arrayed human chromatin | |||||||||
![]() | Purified Arrayed Human Chromatin (Weighted Backprojection) | |||||||||
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![]() | Chromatin / Tomogram / denoise / segmentation / DNA | |||||||||
Biological species | ![]() | |||||||||
Method | electron tomography / cryo EM | |||||||||
![]() | Swulius MT | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Rapid Synthesis of Cryo-ET Data for Training Deep Learning Models. Authors: Purnell C / Heebner J / Hylton RH / Kabonick S / Grillo M / Grigoryev S / Heberle F / Waxham NM / Swulius MT | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 95.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.1 KB 12.1 KB | Display Display | ![]() |
Images | ![]() | 231.9 KB | ||
Filedesc metadata | ![]() | 3.4 KB | ||
Others | ![]() ![]() | 29.4 MB 1016 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 445.9 KB | Display | ![]() |
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Full document | ![]() | 445.5 KB | Display | |
Data in XML | ![]() | 3.1 KB | Display | |
Data in CIF | ![]() | 3.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Purified Arrayed Human Chromatin (Weighted Backprojection) | ||||||||||||||||||||
Voxel size | X=Y=Z: 6.6 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Purified Arrayed Human Chromatin (Regression Denoised)
File | emd_41257_additional_1.map | ||||||||||||
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Annotation | Purified Arrayed Human Chromatin (Regression Denoised) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Additional map: Purified Arrayed Human Chromatin (Greyscale Segmentation)
File | emd_41257_additional_2.map | ||||||||||||
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Annotation | Purified Arrayed Human Chromatin (Greyscale Segmentation) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Purified Arrayed Human Chromatin
Entire | Name: Purified Arrayed Human Chromatin |
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Components |
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-Supramolecule #1: Purified Arrayed Human Chromatin
Supramolecule | Name: Purified Arrayed Human Chromatin / type: complex / ID: 1 / Parent: 0 Details: Weighted Backprojection, Regression Denoised, and Greyscale Segmentation |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | electron tomography |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: Ted Pella / Diameter: 10 nm |
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Electron microscopy
Microscope | TFS KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 5.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 6.0 µm / Nominal defocus min: 6.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Number images used: 24 |
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