ジャーナル: Cell / 年: 2016 タイトル: Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex. 著者: Tiago R D Costa / Aravindan Ilangovan / Marta Ukleja / Adam Redzej / Joanne M Santini / Terry K Smith / Edward H Egelman / Gabriel Waksman / 要旨: Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among ...Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.
ダウンロード / ファイル: emd_4042.map.gz / 形式: CCP4 / 大きさ: 16 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
ボクセルのサイズ
X=Y=Z: 1.1 Å
密度
表面レベル
登録者による: 10.5 / ムービー #1: 10.5
最小 - 最大
-33.032043000000002 - 76.359790000000004
平均 (標準偏差)
1.0478809 (±8.826314999999999)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
128
128
256
Spacing
128
128
256
セル
A: 140.8 Å / B: 140.8 Å / C: 281.6 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.1
1.1
1.1
M x/y/z
128
128
256
origin x/y/z
0.000
0.000
0.000
length x/y/z
140.800
140.800
281.600
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-147
-147
-146
NX/NY/NZ
294
294
294
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
128
128
256
D min/max/mean
-33.032
76.360
1.048
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添付データ
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試料の構成要素
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全体 : F-pilus (pED208)filament made by an assembly of a stoichiometric ...
全体
名称: F-pilus (pED208)filament made by an assembly of a stoichiometric protein-phospholipid complex
要素
細胞器官・細胞要素: F-pilus (pED208)filament made by an assembly of a stoichiometric protein-phospholipid complex
タンパク質・ペプチド: Pilin
リガンド: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
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超分子 #1: F-pilus (pED208)filament made by an assembly of a stoichiometric ...
超分子
名称: F-pilus (pED208)filament made by an assembly of a stoichiometric protein-phospholipid complex タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 / 含まれる分子: #1