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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | 96-nm repeat unit of doublet microtubules from Chlamydomonas reinhardtii flagella | |||||||||
![]() | composite map of the 96 nm repeat from C. reinhardtii | |||||||||
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![]() | cilia / microtubule / dynein / MOTOR PROTEIN | |||||||||
機能・相同性 | ![]() axonemal central pair / axonemal doublet microtubule / inner dynein arm / radial spoke head / axonemal dynein complex assembly / intraciliary transport particle B / radial spoke / positive regulation of cilium-dependent cell motility / inner dynein arm assembly / axonemal dynein complex ...axonemal central pair / axonemal doublet microtubule / inner dynein arm / radial spoke head / axonemal dynein complex assembly / intraciliary transport particle B / radial spoke / positive regulation of cilium-dependent cell motility / inner dynein arm assembly / axonemal dynein complex / outer dynein arm / cilium-dependent cell motility / outer dynein arm assembly / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / establishment of protein localization to organelle / 9+2 motile cilium / intraciliary transport / dynein light chain binding / dynein heavy chain binding / cilium movement / axoneme assembly / motile cilium assembly / axonemal microtubule / cilium organization / blue light photoreceptor activity / dynein axonemal particle / negative regulation of microtubule depolymerization / cell projection organization / nucleoside-diphosphate kinase / 酸化還元酵素 / Set1C/COMPASS complex / ciliary plasm / dynein complex / UTP biosynthetic process / CTP biosynthetic process / minus-end-directed microtubule motor activity / myosin II complex / dynein light intermediate chain binding / microtubule motor activity / cytoplasmic dynein complex / microtubule associated complex / motile cilium / dynein intermediate chain binding / nucleoside diphosphate kinase activity / GTP biosynthetic process / microtubule-based movement / ciliary base / dynein complex binding / beta-tubulin binding / mitotic cytokinesis / : / axoneme / cilium assembly / alpha-tubulin binding / microtubule-based process / enzyme regulator activity / Hsp70 protein binding / FAD binding / regulation of microtubule cytoskeleton organization / GTPase activator activity / acrosomal vesicle / mitotic spindle organization / meiotic cell cycle / cell projection / RNA polymerase II CTD heptapeptide repeat P3 isomerase activity / RNA polymerase II CTD heptapeptide repeat P6 isomerase activity / peptidylprolyl isomerase / cell motility / Hsp90 protein binding / ATP-dependent protein folding chaperone / small GTPase binding / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / mitotic spindle / unfolded protein binding / protein-folding chaperone binding / microtubule binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / microtubule / cytoskeleton / oxidoreductase activity / hydrolase activity / calmodulin binding / ciliary basal body / cilium / cell division / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / identical protein binding / nucleus / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | |||||||||
![]() | Walton T / Brown A | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Axonemal structures reveal mechanoregulatory and disease mechanisms. 著者: Travis Walton / Miao Gui / Simona Velkova / Mahmoud R Fassad / Robert A Hirst / Eric Haarman / Christopher O'Callaghan / Mathieu Bottier / Thomas Burgoyne / Hannah M Mitchison / Alan Brown / ![]() ![]() ![]() ![]() ![]() 要旨: Motile cilia and flagella beat rhythmically on the surface of cells to power the flow of fluid and to enable spermatozoa and unicellular eukaryotes to swim. In humans, defective ciliary motility can ...Motile cilia and flagella beat rhythmically on the surface of cells to power the flow of fluid and to enable spermatozoa and unicellular eukaryotes to swim. In humans, defective ciliary motility can lead to male infertility and a congenital disorder called primary ciliary dyskinesia (PCD), in which impaired clearance of mucus by the cilia causes chronic respiratory infections. Ciliary movement is generated by the axoneme, a molecular machine consisting of microtubules, ATP-powered dynein motors and regulatory complexes. The size and complexity of the axoneme has so far prevented the development of an atomic model, hindering efforts to understand how it functions. Here we capitalize on recent developments in artificial intelligence-enabled structure prediction and cryo-electron microscopy (cryo-EM) to determine the structure of the 96-nm modular repeats of axonemes from the flagella of the alga Chlamydomonas reinhardtii and human respiratory cilia. Our atomic models provide insights into the conservation and specialization of axonemes, the interconnectivity between dyneins and their regulators, and the mechanisms that maintain axonemal periodicity. Correlated conformational changes in mechanoregulatory complexes with their associated axonemal dynein motors provide a mechanism for the long-hypothesized mechanotransduction pathway to regulate ciliary motility. Structures of respiratory-cilia doublet microtubules from four individuals with PCD reveal how the loss of individual docking factors can selectively eradicate periodically repeating structures. | |||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
-関連構造データ
関連構造データ | ![]() 8glvMC ![]() 8j07C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | composite map of the 96 nm repeat from C. reinhardtii | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 これらの図は立方格子座標系で作成されたものです | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.36 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
+追加マップ: position 1 of DMT - focused refinement 1 - postprocess
+追加マップ: position 1 of DMT - focused refinement 3 - postprocess
+追加マップ: position 4 of DMT - focused refinement 4 - postprocess
+追加マップ: position 4 of DMT - focused refinement 4 - mask
+追加マップ: position 4 of DMT - focused refinement 3 - postprocess
+追加マップ: position 4 of DMT - focused refinement 3 - half map 1
+追加マップ: position 4 of DMT - focused refinement 3 - half map 2
+追加マップ: position 4 of DMT - focused refinement 4 - half map 1
+追加マップ: position 4 of DMT - focused refinement 1 - mask
+追加マップ: position 4 of DMT - focused refinement 3 - mask
+追加マップ: position 4 of DMT - focused refinement 1 - postprocess
+追加マップ: position 4 of DMT - focused refinement 2 - postprocess
+追加マップ: position 1 of DMT - focused refinement 3 - half map 2
+追加マップ: position 4 of DMT - focused refinement 2 - half map 1
+追加マップ: position 4 of DMT - focused refinement 2 - half map 2
+追加マップ: position 4 of DMT - focused refinement 2 - mask
+追加マップ: position 4 of DMT - focused refinement 6 - half map 2
+追加マップ: position 4 of DMT - focused refinement 7 - half map 1
+追加マップ: position 4 of DMT - focused refinement 6 - postprocess
+追加マップ: position 4 of DMT - focused refinement 5 - postprocess
+追加マップ: position 4 of DMT - focused refinement 5 - mask
+追加マップ: position 4 of DMT - focused refinement 7 - mask
+追加マップ: position 4 of DMT - focused refinement 7 - postprocess
+追加マップ: position 1 of DMT - focused refinement 3 - mask
+追加マップ: position 4 of DMT - focused refinement 8 - half map 1
+追加マップ: position 4 of DMT - focused refinement 5 - half map 1
+追加マップ: position 4 of DMT - focused refinement 8 - half map 2
+追加マップ: position 4 of DMT - focused refinement 6 - half map 1
+追加マップ: position 4 of DMT - focused refinement 8 - mask
+追加マップ: position 4 of DMT - focused refinement 8 - postprocess
+追加マップ: position 4 of DMT - focused refinement 5 - half map 2
+追加マップ: position 4 of DMT - focused refinement 7 - half map 2
+追加マップ: position 4 of DMT - focused refinement 6 - mask
+追加マップ: NDRC closed conformation consensus - postprocess
+追加マップ: position 1 of DMT - focused refinement 4 - postprocess
+追加マップ: N-DRC baseplate - focused refinement 3 - postprocess
+追加マップ: ODA1 core connection with IDAf - half map 2
+追加マップ: ODA1 core connection with IDAf - mask
+追加マップ: N-DRC linker - distal lobe - half map 2
+追加マップ: NDRC closed conformation consensus - half map 1
+追加マップ: N-DRC linker - central region - mask
+追加マップ: N-DRC baseplate - focused refinement 1 - half map 1
+追加マップ: N-DRC linker - proximal lobe - mask
+追加マップ: ODA beta heavy chain motor - half map 2
+追加マップ: ODA beta heavy chain motor - half map 1
+追加マップ: position 1 of DMT - focused refinement 4 - half map 2
+追加マップ: N-DRC baseplate - focused refinement 2 - half map 2
+追加マップ: ODA2 core connection with MIA - half map 2
+追加マップ: N-DRC linker - distal lobe - postprocess
+追加マップ: N-DRC baseplate - focused refinement 3 - mask
+追加マップ: ODA beta heavy chain motor - mask
+追加マップ: ODA2 core connection with MIA - mask
+追加マップ: NDRC open conformation consensus - half map 1
+追加マップ: NDRC open conformation - linker - mask
+追加マップ: N-DRC linker - central region - half map 1
+追加マップ: N-DRC linker - proximal lobe - half map 2
+追加マップ: position 1 of DMT - focused refinement 4 - mask
+追加マップ: N-DRC baseplate - focused refinement 1 - half map 2
+追加マップ: ODA3 core connection with N-DRC - half map 2
+追加マップ: ODA3 core connection with N-DRC - mask
+追加マップ: N-DRC linker - distal lobe - half map 1
+追加マップ: NDRC closed conformation consensus - mask
+追加マップ: N-DRC linker - proximal lobe - half map 1
+追加マップ: N-DRC linker - proximal lobe - postprocess
+追加マップ: N-DRC baseplate - focused refinement 2 - half map 1
+追加マップ: NDRC open conformation consensus - mask
+追加マップ: N-DRC baseplate - focused refinement 2 - postprocess
+追加マップ: position 1 of DMT - focused refinement 3 - half map 1
+追加マップ: N-DRC baseplate - focused refinement 3 - half map 1
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+追加マップ: N-DRC baseplate - focused refinement 2 - mask
+追加マップ: N-DRC baseplate - focused refinement 1 - postprocess
+追加マップ: N-DRC linker - central region - postprocess
+追加マップ: N-DRC linker - distal lobe - mask
+追加マップ: N-DRC baseplate - focused refinement 3 - half map 2
+追加マップ: ODA3 core connection with N-DRC - postprocess
+追加マップ: NDRC open conformation - linker - half map 1
+追加マップ: NDRC open conformation - linker - half map 2
+追加マップ: position 1 of DMT - focused refinement 5 - half map 2
+追加マップ: N-DRC linker - central region - half map 2
+追加マップ: N-DRC baseplate - focused refinement 1 - mask
+追加マップ: ODA2 core connection with MIA - postprocess
+追加マップ: ODA3 core connection with N-DRC - half map 1
+追加マップ: NDRC open conformation consensus - half map 2
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+追加マップ: ODA2 core connection with MIA - half map 1
+追加マップ: ODA1 core connection with IDAf - half map 1
+追加マップ: position 1 of DMT - focused refinement 7 - half map 2
+追加マップ: NDRC open conformation consensus - postprocess
+追加マップ: Tetherhead complex connection with inner junction - half map 1
+追加マップ: Tetherhead complex connection with inner junction - postprocess
+追加マップ: IDAa base - postprocess
+追加マップ: Tetherhead complex connection with IDAf motors - mask
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+追加マップ: Tetherhead complex connection with IDAf motors - half map 1
+追加マップ: IDAa base - half map 2
+追加マップ: Tetherhead complex connection with IDAf motors - half map 2
+追加マップ: Tetherhead complex connection with inner junction - half map 2
+追加マップ: position 1 of DMT - focused refinement 6 - postprocess
+追加マップ: IDAa base - mask
+追加マップ: Tetherhead complex connection with IDAf motors - postprocess
+追加マップ: Tetherhead complex connection with inner junction - mask
+追加マップ: IDAf distal half - focused refinement 2 - half map 2
+追加マップ: MIA / FAP57 connection - half map 1
+追加マップ: IDAa motor - postprocess
+追加マップ: MIA / FAP57 connection - half map 2
+追加マップ: IDAc - half map 1
+追加マップ: IDAa motor - half map 2
+追加マップ: IDAa motor - mask
+追加マップ: position 1 of DMT - focused refinement 1 - half map 1
+追加マップ: position 1 of DMT - focused refinement 6 - half map 2
+追加マップ: IDAf IC/LC region - half map 2
+追加マップ: MIA / FAP57 connection - mask
+追加マップ: IDAb - half map 2
+追加マップ: MIA / FAP189 connection - mask
+追加マップ: IDAc - postprocess
+追加マップ: IDAf distal half - focused refinement 2 - half map 1
+追加マップ: MIA / FAP57 connection - postprocess
+追加マップ: IDAd motor - half map 1
+追加マップ: IDAd base with RS3S - half map 1
+追加マップ: IDAc - mask
+追加マップ: position 1 of DMT - focused refinement 8 - postprocess
+追加マップ: IDAe - half map 1
+追加マップ: MIA / FAP189 connection - half map 2
+追加マップ: IDAf distal half - focused refinement 1 - mask
+追加マップ: IDAb - mask
+追加マップ: IDAf distal half - focused refinement 1 - postprocess
+追加マップ: MIA / FAP189 connection - postprocess
+追加マップ: IDAf distal half - focused refinement 1 - half map 1
+追加マップ: IDAe - mask
+追加マップ: IDAb - postprocess
+追加マップ: IDAf distal half - focused refinement 2 - postprocess
+追加マップ: position 1 of DMT - focused refinement 5 - postprocess
+追加マップ: MIA / FAP189 connection - half map 1
+追加マップ: IDAf distal half - focused refinement 2 - mask
+追加マップ: IDAd motor - mask
+追加マップ: IDAd base with RS3S - postprocess
+追加マップ: IDAf IC/LC region - half map 1
+追加マップ: IDAe - postprocess
+追加マップ: IDAd base with RS3S - half map 2
+追加マップ: IDAb - half map 1
+追加マップ: IDAc - half map 2
+追加マップ: IDAe - half map 2
+追加マップ: position 1 of DMT - focused refinement 8 - mask
+追加マップ: IDAa motor - half map 1
+追加マップ: IDAd base with RS3S - mask
+追加マップ: IDAf IC/LC region - mask
+追加マップ: IDAd motor - postprocess
+追加マップ: IDAd motor - half map 2
+追加マップ: IDAf IC/LC region - postprocess
+追加マップ: IDAf distal half - focused refinement 1 - half map 2
+追加マップ: IDAg motor - half map 1
+追加マップ: IDAg base - postprocess
+追加マップ: distal protrusion - half map 1
+追加マップ: position 1 of DMT - focused refinement 6 - mask
+追加マップ: IDAf closed conformation - core - half map 1
+追加マップ: IDAf open conformation - motors - postprocess
+追加マップ: IDAf open conformation - core - half map 2
+追加マップ: IDAf closed conformation - motors - half map 2
+追加マップ: IDAf closed conformation - motors - mask
+追加マップ: IDAf open conformation - core - postprocess
+追加マップ: IDAf open conformation - motors - mask
+追加マップ: IDAf open conformation - core - half map 1
+追加マップ: IDAf open conformation - core - mask
+追加マップ: IDAg base - half map 1
+追加マップ: position 1 of DMT - focused refinement 8 - half map 2
+追加マップ: IDAf closed conformation - motors - half map 1
+追加マップ: distal protrusion - mask
+追加マップ: IDAf closed conformation - core - postprocess
+追加マップ: IDAf open conformation - motors - half map 2
+追加マップ: IDAg base - mask
+追加マップ: IDAf open conformation - motors - half map 1
+追加マップ: IDAf closed conformation - motors - postprocess
+追加マップ: IDAf closed conformation - core - mask
+追加マップ: IDAg base - half map 2
+追加マップ: IDAg base - half map 2
+追加マップ: position 1 of DMT - focused refinement 7 - mask
+追加マップ: IDAf closed conformation - core - half map 2
+追加マップ: distal protrusion - half map 2
+追加マップ: distal protrusion - postprocess
+追加マップ: IDAg base - mask
+追加マップ: IDAg base - postprocess
+追加マップ: position 1 of DMT - focused refinement 6 - half map 1
+追加マップ: position 1 of DMT - focused refinement 7 - half map 1
+追加マップ: position 1 of DMT - focused refinement 5 - half map 1
+追加マップ: position 1 of DMT - focused refinement 2 - postprocess
+追加マップ: position 1 of DMT - focused refinement 5 - mask
+追加マップ: position 1 of DMT - focused refinement 7 - postprocess
+追加マップ: position 1 of DMT - focused refinement 8 - half map 1
+追加マップ: position 2 of DMT - focused refinement 1 - postprocess
+追加マップ: position 2 of DMT - focused refinement 1 - half map 1
+追加マップ: position 2 of DMT - focused refinement 1 - mask
+追加マップ: position 2 of DMT - focused refinement 1 - half map 2
+追加マップ: position 2 of DMT - focused refinement 4 - postprocess
+追加マップ: position 2 of DMT - focused refinement 4 - mask
+追加マップ: position 2 of DMT - focused refinement 4 - half map 2
+追加マップ: position 1 of DMT - focused refinement 1 - mask
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+追加マップ: position 2 of DMT - focused refinement 3 - half map 1
+追加マップ: position 2 of DMT - focused refinement 3 - mask
+追加マップ: position 2 of DMT - focused refinement 2 - mask
+追加マップ: position 2 of DMT - focused refinement 2 - postprocess
+追加マップ: position 2 of DMT - focused refinement 4 - half map 1
+追加マップ: position 2 of DMT - focused refinement 2 - half map 1
+追加マップ: position 2 of DMT - focused refinement 2 - half map 2
+追加マップ: position 2 of DMT - focused refinement 3 - postprocess
+追加マップ: position 2 of DMT - focused refinement 7 - mask
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+追加マップ: position 2 of DMT - focused refinement 8 - postprocess
+追加マップ: position 2 of DMT - focused refinement 8 - half map 2
+追加マップ: position 2 of DMT - focused refinement 7 - half map 1
+追加マップ: position 2 of DMT - focused refinement 5 - half map 1
+追加マップ: position 2 of DMT - focused refinement 5 - half map 2
+追加マップ: position 2 of DMT - focused refinement 8 - half map 1
+追加マップ: position 2 of DMT - focused refinement 5 - mask
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+追加マップ: position 2 of DMT - focused refinement 6 - half map 2
+追加マップ: position 2 of DMT - focused refinement 6 - postprocess
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+追加マップ: position 2 of DMT - focused refinement 7 - postprocess
+追加マップ: position 2 of DMT - focused refinement 8 - mask
+追加マップ: position 2 of DMT - focused refinement 6 - mask
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+追加マップ: position 3 of DMT - focused refinement 3 - mask
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+追加マップ: position 1 of DMT - focused refinement 1 - half map 2
+追加マップ: position 3 of DMT - focused refinement 3 - half map 2
+追加マップ: position 3 of DMT - focused refinement 2 - mask
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+追加マップ: position 3 of DMT - focused refinement 4 - half map 1
+追加マップ: position 3 of DMT - focused refinement 4 - half map 2
+追加マップ: position 3 of DMT - focused refinement 2 - postprocess
+追加マップ: position 3 of DMT - focused refinement 1 - mask
+追加マップ: position 3 of DMT - focused refinement 2 - half map 1
+追加マップ: position 3 of DMT - focused refinement 1 - half map 2
+追加マップ: position 3 of DMT - focused refinement 1 - half map 1
+追加マップ: position 1 of DMT - focused refinement 2 - mask
+追加マップ: position 3 of DMT - focused refinement 2 - half map 2
+追加マップ: position 3 of DMT - focused refinement 7 - mask
+追加マップ: position 3 of DMT - focused refinement 7 - postprocess
+追加マップ: position 3 of DMT - focused refinement 5 - mask
+追加マップ: position 3 of DMT - focused refinement 6 - postprocess
+追加マップ: position 3 of DMT - focused refinement 6 - mask
+追加マップ: position 3 of DMT - focused refinement 8 - mask
+追加マップ: position 3 of DMT - focused refinement 8 - postprocess
+追加マップ: position 3 of DMT - focused refinement 5 - postprocess
+追加マップ: position 3 of DMT - focused refinement 7 - half map 1
+追加マップ: position 1 of DMT - focused refinement 4 - half map 1
+追加マップ: position 3 of DMT - focused refinement 5 - half map 1
+追加マップ: position 3 of DMT - focused refinement 8 - half map 2
+追加マップ: position 3 of DMT - focused refinement 8 - half map 1
+追加マップ: position 3 of DMT - focused refinement 7 - half map 1
+追加マップ: position 3 of DMT - focused refinement 5 - half map 2
+追加マップ: position 3 of DMT - focused refinement 6 - half map 1
+追加マップ: position 3 of DMT - focused refinement 6 - half map 2
+追加マップ: position 4 of DMT - focused refinement 1 - half map 1
+追加マップ: position 4 of DMT - focused refinement 4 - half map 2
+追加マップ: position 4 of DMT - focused refinement 1 - half map 2
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試料の構成要素
+全体 : 96-nm repeat unit of doublet microtubules from the flagella of Ch...
+超分子 #1: 96-nm repeat unit of doublet microtubules from the flagella of Ch...
+分子 #1: Tubulin beta
+分子 #2: Tubulin alpha
+分子 #3: PACRG
+分子 #4: Cilia- and flagella-associated protein 20
+分子 #5: Meiosis-specific nuclear structural protein 1
+分子 #6: Flagellar associated protein
+分子 #7: Coiled-coil protein associated with protofilament ribbons
+分子 #8: Cilia- and flagella-associated protein 45
+分子 #9: FAP34
+分子 #10: Flagellar associated protein
+分子 #11: Protein Flattop homolog
+分子 #12: FAP52
+分子 #13: Nucleoside diphosphate kinase
+分子 #14: Flagellar associated protein
+分子 #15: Flagellar associated protein
+分子 #16: Flagellar associated protein
+分子 #17: FAP143
+分子 #18: Flagellar associated protein
+分子 #19: FAP166
+分子 #20: Flagellar associated protein
+分子 #21: FAP339
+分子 #22: Protofilament ribbon protein of flagellar microtubules
+分子 #23: Flagellar associated protein
+分子 #24: Flagellar associated protein
+分子 #25: Flagellar associated protein
+分子 #26: Flagellar associated protein
+分子 #27: FAP129
+分子 #28: FAP21
+分子 #29: Flagellar associated protein
+分子 #30: Flagellar associated protein
+分子 #31: FAP306
+分子 #32: Flagellar associated protein
+分子 #33: Flagellar associated protein
+分子 #34: Dynein axonemal light chain 1
+分子 #35: Outer dynein arm-docking complex subunit 1
+分子 #36: Outer dynein arm-docking complex protein DC3
+分子 #37: Dynein gamma chain, flagellar outer arm
+分子 #38: IC97/Casc1 N-terminal domain-containing protein
+分子 #39: Axonemal inner arm I1 intermediate chain dynein IC138
+分子 #40: IC140
+分子 #41: Axonemal inner arm dynein I1/f subunit
+分子 #42: Dynein light chain roadblock LC7b
+分子 #43: Dynein light chain roadblock LC7a
+分子 #44: Dynein 8 kDa light chain, flagellar outer arm
+分子 #45: Dynein light chain Tctex1
+分子 #46: Inner arm dynein light chain
+分子 #47: Dynein light chain 10
+分子 #48: Cilia- and flagella-associated protein 73
+分子 #49: Cilia- and flagella-associated protein 100
+分子 #50: Dynein, 78 kDa intermediate chain, flagellar outer arm
+分子 #51: WD_REPEATS_REGION domain-containing protein
+分子 #52: Flagellar outer dynein arm light chain 2
+分子 #53: Dynein 18 kDa light chain, flagellar outer arm
+分子 #54: Dynein 11 kDa light chain, flagellar outer arm
+分子 #55: Dynein light chain 9
+分子 #56: Outer dynein arm protein 1
+分子 #57: AAA+ ATPase domain-containing protein
+分子 #58: Dynein-1-beta heavy chain, flagellar inner arm I1 complex
+分子 #59: WD_REPEATS_REGION domain-containing protein
+分子 #60: Inner-arm dynein f/I1 light chain
+分子 #61: Heavy chain alpha
+分子 #62: Flagellar outer dynein arm heavy chain beta
+分子 #63: Dynein 14 kDa light chain, flagellar outer arm
+分子 #64: Dynein 16 kDa light chain, flagellar outer arm
+分子 #65: FAP44
+分子 #66: FAP43
+分子 #67: Dynein regulatory complex protein 1
+分子 #68: Dynein regulatory complex subunit 2
+分子 #69: Dynein regulatory complex subunit 6
+分子 #70: Dynein regulatory complex subunit 4
+分子 #71: Dynein regulatory complex protein 8
+分子 #72: ATPase AAA-type core domain-containing protein
+分子 #73: Flagellar associated protein
+分子 #74: Dynein regulatory complex subunit 3
+分子 #75: Dynein regulatory complex protein 9
+分子 #76: Dynein regulatory complex protein 10
+分子 #77: DRC5
+分子 #78: DUF4201 domain-containing protein
+分子 #79: Calmodulin
+分子 #80: Caltractin
+分子 #81: AAA+ ATPase domain-containing protein
+分子 #82: DUF4201 domain-containing protein
+分子 #83: Cilia- and flagella-associated protein 299
+分子 #84: Cilia- and flagella-associated protein 53
+分子 #85: FAP210
+分子 #86: Actin
+分子 #87: Flagellar radial spoke protein 5
+分子 #88: Flagellar radial spoke protein 1
+分子 #89: Flagellar radial spoke protein 2
+分子 #90: Radial spoke protein 3
+分子 #91: Radial spoke protein 4
+分子 #92: Flagellar radial spoke protein 6
+分子 #93: Radial spoke protein 7
+分子 #94: Radial spoke protein 9
+分子 #95: Radial spoke protein 10
+分子 #96: Radial spoke protein 11
+分子 #97: Peptidyl-prolyl cis-trans isomerase
+分子 #98: Radial spoke protein 14
+分子 #99: Radial spoke protein 16
+分子 #100: UNKNOWN
+分子 #101: Nucleoside diphosphate kinase 6
+分子 #102: Cytochrome b5 heme-binding domain-containing protein
+分子 #103: FAP385
+分子 #104: FAP207
+分子 #105: FAP253
+分子 #106: 28 kDa inner dynein arm light chain, axonemal
+分子 #107: AAA+ ATPase domain-containing protein
+分子 #108: UNKNOWN
+分子 #109: Radial spoke protein 15
+分子 #110: Radial spike protein 8
+分子 #111: DHC_N2 domain-containing protein
+分子 #112: Flagellar-associated protein 59
+分子 #113: Flagellar-associated protein 172
+分子 #114: Flagellar associated protein
+分子 #115: Cilia- and flagella-associated protein 58
+分子 #116: FAP251
+分子 #117: Cilia- and flagella-associated protein 61
+分子 #118: DHC2
+分子 #119: Subunit of axonemal inner dynein
+分子 #120: Subunit of axonemal inner dynein
+分子 #121: CFAP91 domain-containing protein
+分子 #122: AAA+ ATPase domain-containing protein
+分子 #123: AAA+ ATPase domain-containing protein
+分子 #124: Cilia- and flagella-associated protein 77
+分子 #125: Flagellar associated protein
+分子 #126: Flagellar associated protein
+分子 #127: FAP1
+分子 #128: GUANOSINE-5'-TRIPHOSPHATE
+分子 #129: GUANOSINE-5'-DIPHOSPHATE
+分子 #130: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | filament |
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試料調製
濃度 | 20 mg/mL | |||||||||||||||
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緩衝液 | pH: 7.4 構成要素:
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グリッド | モデル: C-flat-1.2/1.3 / 材質: COPPER / 前処理 - タイプ: GLOW DISCHARGE | |||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 298 K / 装置: FEI VITROBOT MARK IV 詳細: 2.5 microliters of splayed axoneme sample at 16-26 mg/mL were applied to glow-discharged C-Flat 1.2/1.3-4Cu grids at 100% humidity. The samples were then incubated 10 s, blotted for 10 s, and ...詳細: 2.5 microliters of splayed axoneme sample at 16-26 mg/mL were applied to glow-discharged C-Flat 1.2/1.3-4Cu grids at 100% humidity. The samples were then incubated 10 s, blotted for 10 s, and plunged into liquid ethane. | |||||||||||||||
詳細 | Axonemes were splayed using 10 mM ATP and 0.75 mM CaCl2 for one hour at room temperature before centrifugation and resuspension. |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 実像数: 36918 / 平均露光時間: 3.7 sec. / 平均電子線量: 61.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 64000 |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |