PDB-8j07: 96nm repeat of human respiratory doublet microtubule and associat...

Basic information

• Entry: Database: PDB / ID: 8j07
• Title: 96nm repeat of human respiratory doublet microtubule and associated axonemal complexes

Components

• (Cilia- and flagella-associated protein ...) x 21
• (Coiled-coil domain-containing protein ...) x 4
• (Dynein axonemal heavy chain ...) x 9
• (Dynein axonemal intermediate chain ...) x 5
• (Dynein light chain Tctex-type ...) x 2
• (Dynein light chain roadblock-type ...) x 2
• (Dynein regulatory complex protein ...) x 5
• (Dynein regulatory complex subunit ...) x 5
• (EF-hand domain-containing family member ...) x 2
• (Nucleoside diphosphate kinase ...) x 2
• (Outer dynein arm-docking complex subunit ...) x 4
• (Piercer of microtubule wall ...) x 2
• (RIB43A-like with coiled-coils protein ...) x 2
• (Radial spoke head ...) x 5
• (Uncharacterized protein ...) x 2
• ACTA2
• Axonemal dynein light intermediate polypeptide 1
• Calaxin
• Calmodulin-1
• Centrin-2
• Cilia- and flagella- associated protein 210
• Cytochrome b5 domain-containing protein 1
• DPY30 domain-containing protein 2
• DYNLL1
• DnaJ homolog subfamily B member 13
• Dynein axonemal light chain 1
• EF-hand domain-containing protein 1
• Enkurin
• IQ and ubiquitin-like domain-containing protein
• Leucine-rich repeat-containing protein 34
• MORN repeat-containing protein 3
• Meiosis-specific nuclear structural protein 1
• Parkin coregulated gene protein
• Probable inactive peptidyl-prolyl cis-trans isomerase-like 6
• Protein FAM166B
• Protein FAM166C
• Protein Flattop
• Ropporin-1-like protein
• Sperm acrosome-associated protein 9
• Sperm surface protein Sp17
• Sperm-associated antigen 8
• Tektin-1
• Tektin-2
• Tetratricopeptide repeat protein 29
• Thioredoxin domain-containing protein 6
• Tubulin alpha-1A chain
• Tubulin beta-4B chain
• Unknown
• Zinc finger MYND domain-containing protein 12

• Keywords: STRUCTURAL PROTEIN / cilia / microtubule / dynein

Function / homology

Function:

distal portion of axoneme / sperm mitochondrial sheath assembly / determination of pancreatic left/right asymmetry / maintenance of ciliary planar beating movement pattern / radial spoke head 2 / determination of digestive tract left/right asymmetry / regulation of cilium beat frequency / radial spoke assembly / outer dynein arm docking complex / radial spoke head 1 / radial spoke head 3 / regulation of intraciliary retrograde transport / determination of heart left/right asymmetry / axonemal doublet microtubule / : / outer dense fiber / regulation of cilium movement / inner dynein arm / radial spoke head / mucociliary clearance / axonemal microtubule doublet inner sheath / mating / epithelial cilium movement involved in determination of left/right asymmetry / axonemal central apparatus assembly / establishment of left/right asymmetry / 9+0 motile cilium / axonemal dynein complex assembly / protein localization to motile cilium / sperm flagellum assembly / intracellular transport of viral protein in host cell / sperm fibrous sheath / secretory vesicle / radial spoke / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / manchette / regulation of flagellated sperm motility / sperm axoneme assembly / protein polyglutamylation / inner dynein arm assembly / axonemal dynein complex / outer dynein arm / regulation of calcineurin-NFAT signaling cascade / positive regulation of feeding behavior / regulation of microtubule nucleation / XPC complex / microtubule severing ATPase activity / cilium-dependent cell motility / outer dynein arm assembly / kinocilium / Arp2/3 complex binding / cerebrospinal fluid circulation / sperm principal piece / determination of liver left/right asymmetry / intraciliary retrograde transport / regulation of cilium beat frequency involved in ciliary motility / lateral ventricle development / epithelial cilium movement involved in extracellular fluid movement / visual behavior / cilium movement involved in cell motility / regulation of store-operated calcium entry / Transferases; Transferring phosphorus-containing groups / 9+2 motile cilium / sperm head-tail coupling apparatus / intraciliary transport / microtubule sliding / dynein light chain binding / regulation of cilium assembly / ciliary transition zone / dynein heavy chain binding / cilium movement / photoreceptor connecting cilium / axoneme assembly / motile cilium assembly / Post-chaperonin tubulin folding pathway / left/right axis specification / organelle transport along microtubule / nucleoside metabolic process / calcium ion sensor activity / negative regulation of Arp2/3 complex-mediated actin nucleation / axonemal microtubule / heterotrimeric G-protein binding / Cilium Assembly / cytoskeleton-dependent intracellular transport / transcription export complex 2 / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / cilium organization / Carboxyterminal post-translational modifications of tubulin / ciliary tip / Activation of SMO / positive regulation of protein localization to cilium / forebrain morphogenesis / gamma-tubulin ring complex / ventricular system development / glial cell differentiation / dynein axonemal particle / Intraflagellar transport / neuron projection arborization / Sealing of the nuclear envelope (NE) by ESCRT-III

Domain/homology:

CASC1, C-terminal / Cancer susceptibility candidate 1 C-terminal / GSKIP domain superfamily / Sperm surface protein Sp17 / : / : / : / Cilia- and flagella-associated protein 61, N-terminal domain / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 61, N-terminal domain / Nucleoside-diphosphate kinase-like NDK-H5 / Dynein-f, intermediate chain IC97/Dnai7-like / Domain of unknown function DUF4200 / IC97/Casc1, N-terminal / : / : / : / : / : / Domain of unknown function (DUF4200) / Cancer susceptibility candidate 1 N-terminus / CFAP58 central coiled coil / Protein of unknown function DUF4464 / Domain of unknown function (DUF4464) / Axonemal dynein light chain / Dynein regulatory complex protein 1, C-terminal / Outer dynein arm-docking complex subunit 4 / : / : / Axonemal dynein light chain / Sperm tail C-terminal domain / Protein of unknown function DUF1143 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Radial spoke protein 14 / : / : / Cilia- and flagella-associated protein 68 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / Protein of unknown function DUF4541 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / : / : / : / CCDC113/CCDC96, coiled-coil / Cilia- and flagella-associated protein 91 / Cilia and flagella associated protein 90 / IQ and ubiquitin-like domain-containing protein / Growth arrest-specific protein 8 domain / Outer dynein arm-docking complex subunit 3 / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / : / Growth-arrest specific micro-tubule binding / Sperm tail / Radial spokehead-like protein / Radial spoke 3 / FAM183 family / : / Radial spokehead-like protein / Radial spoke protein 3 / FAM183A and FAM183B related / : / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / : / : / Protein CFAP95 / ODAD1 central coiled coil region / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / BRE1 E3 ubiquitin ligase / Tektin / Cilia- and flagella-associated protein 276 / Sperm acrosome-associated protein 9 / : / Tektin family / Protein of unknown function (DUF3695) / Sperm acrosome-associated protein 9 / Sdc1/DPY30 / Uncharacterised protein FAM166/UPF0605 / Cilia- and flagella-associated protein 77 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Cilia- and flagella-associated protein 77 / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 161-like domain / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 141

Component:

GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Dynein axonemal heavy chain 10 / Cilia- and flagella-associated protein 90 / Outer dynein arm-docking complex subunit 3 / Cilia- and flagella-associated protein 73 / Ciliary microtubule inner protein 2C / Cilia- and flagella-associated protein 144 / Ciliary microtubule inner protein 2B / Dynein axonemal heavy chain 12 / Piercer of microtubule wall 2 protein / Axonemal dynein light intermediate polypeptide 1 / Dynein regulatory complex subunit 4 / Calmodulin-1 / Centrin-2 / Nucleoside diphosphate kinase homolog 5 / DnaJ homolog subfamily B member 13 / Dynein light chain Tctex-type 1 / Tubulin beta-4B chain / Cilia- and flagella- associated protein 210 / Sperm surface protein Sp17 / Cilia- and flagella-associated protein 184 / Cilia- and flagella-associated protein 100 / Coiled-coil domain-containing protein 40 / Dynein axonemal light chain 1 / Piercer of microtubule wall 1 protein / EF-hand domain-containing family member C2 / Dynein regulatory complex subunit 5 / EF-hand domain-containing protein 1 / Outer dynein arm-docking complex subunit 2 / Cilia- and flagella-associated protein 276 / Cilia- and flagella-associated protein 58 / Radial spoke head protein 4 homolog A / Protein Flattop / Dynein axonemal intermediate chain 4 / Cilia- and flagella-associated protein 95 / Cilia- and flagella-associated protein 141 / Dynein regulatory complex protein 8 / Cilia- and flagella-associated protein 161 / Cytochrome b5 domain-containing protein 1 / MORN repeat-containing protein 3 / Dynein axonemal intermediate chain 7 / Cilia- and flagella-associated protein 299 / Cilia- and flagella-associated protein 77 / Tubulin alpha-1A chain / Cilia- and flagella-associated protein 91 / Radial spoke head protein 3 homolog / Dynein regulatory complex protein 11 / Thioredoxin domain-containing protein 6 / Dynein axonemal intermediate chain 3 / Dynein regulatory complex subunit 2 / Probable inactive peptidyl-prolyl cis-trans isomerase-like 6 / Dynein regulatory complex subunit 7 / Leucine-rich repeat-containing protein 34 / Cilia- and flagella-associated protein 107 / Cilia- and flagella-associated protein 52 / RIB43A-like with coiled-coils protein 1 / EF-hand domain-containing family member B / IQ motif and ubiquitin-like domain-containing protein / Tetratricopeptide repeat protein 29 / Cilia- and flagella-associated protein 43 / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 251 / Enkurin / Dynein axonemal heavy chain 3 / Dynein axonemal heavy chain 5 / Dynein light chain roadblock-type 2 / Dynein light chain Tctex-type protein 2B / Dynein axonemal heavy chain 7 / Radial spoke head 1 homolog / Tektin-1 / Ropporin-1-like protein / Dynein regulatory complex protein 10 / Sperm acrosome-associated protein 9 / DPY30 domain-containing protein 2 / Outer dynein arm-docking complex subunit 1 / Cilia- and flagella-associated protein 53 / Parkin coregulated gene protein / Dynein regulatory complex protein 1 / Cilia- and flagella-associated protein 57 / Cilia- and flagella-associated protein 44 / Outer dynein arm-docking complex subunit 4 / Sperm-associated antigen 8 / Dynein axonemal heavy chain 6 / Dynein axonemal intermediate chain 2 / Dynein regulatory complex subunit 3 / Dynein regulatory complex protein 9 / Zinc finger MYND domain-containing protein 12 / Cilia- and flagella-associated protein 263 / Radial spoke head protein 9 homolog / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 68 / Calaxin / Dynein light chain roadblock-type 1 / Dynein axonemal heavy chain 9 / Dynein axonemal heavy chain 2 / Dynein axonemal heavy chain 1 / Coiled-coil domain-containing protein 39 / Radial spoke head 14 homolog

• Biological species: Homo sapiens (human)
• Method: ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.1 Å
• Authors: Gui, M. / Brown, A.

Funding support

United States, 2items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM141109	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM143183	United States

• Citation: Journal: Nature / Year: 2023; Title: Axonemal structures reveal mechanoregulatory and disease mechanisms.; Authors: Travis Walton / Miao Gui / Simona Velkova / Mahmoud R Fassad / Robert A Hirst / Eric Haarman / Christopher O'Callaghan / Mathieu Bottier / Thomas Burgoyne / Hannah M Mitchison / Alan Brown / ; Abstract: Motile cilia and flagella beat rhythmically on the surface of cells to power the flow of fluid and to enable spermatozoa and unicellular eukaryotes to swim. In humans, defective ciliary motility can lead to male infertility and a congenital disorder called primary ciliary dyskinesia (PCD), in which impaired clearance of mucus by the cilia causes chronic respiratory infections. Ciliary movement is generated by the axoneme, a molecular machine consisting of microtubules, ATP-powered dynein motors and regulatory complexes. The size and complexity of the axoneme has so far prevented the development of an atomic model, hindering efforts to understand how it functions. Here we capitalize on recent developments in artificial intelligence-enabled structure prediction and cryo-electron microscopy (cryo-EM) to determine the structure of the 96-nm modular repeats of axonemes from the flagella of the alga Chlamydomonas reinhardtii and human respiratory cilia. Our atomic models provide insights into the conservation and specialization of axonemes, the interconnectivity between dyneins and their regulators, and the mechanisms that maintain axonemal periodicity. Correlated conformational changes in mechanoregulatory complexes with their associated axonemal dynein motors provide a mechanism for the long-hypothesized mechanotransduction pathway to regulate ciliary motility. Structures of respiratory-cilia doublet microtubules from four individuals with PCD reveal how the loss of individual docking factors can selectively eradicate periodically repeating structures.

History

Deposition	Apr 9, 2023	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Jul 26, 2023	Provider: repository / Type: Initial release
Revision 1.1	Aug 2, 2023	Group: Database references / Category: pdbx_database_related

Assembly

Deposited unit

0: Dynein regulatory complex subunit 4

1: Dynein regulatory complex protein 1

10: Dynein regulatory complex protein 10

11: Dynein regulatory complex protein 11

12: Dynein regulatory complex protein 8

13: Dynein regulatory complex protein 8

14: Dynein regulatory complex protein 8

1A: EF-hand domain-containing family member B

1B: EF-hand domain-containing family member B

1C: EF-hand domain-containing family member B

1D: Cilia- and flagella-associated protein 45

1E: Cilia- and flagella-associated protein 45

1F: Cilia- and flagella-associated protein 45

1G: Cilia- and flagella-associated protein 45

1H: Cilia- and flagella-associated protein 45

1I: Cilia- and flagella-associated protein 45

1J: Cilia- and flagella-associated protein 45

1L: Cilia- and flagella-associated protein 52

1M: Cilia- and flagella-associated protein 52

1N: Cilia- and flagella-associated protein 52

1O: Cilia- and flagella-associated protein 52

1P: Cilia- and flagella-associated protein 52

1Q: Cilia- and flagella-associated protein 52

1R: Cilia- and flagella-associated protein 53

1S: Cilia- and flagella-associated protein 53

1T: Cilia- and flagella-associated protein 53

1V: Cilia- and flagella-associated protein 95

1W: Cilia- and flagella-associated protein 95

1Y: Cilia- and flagella-associated protein 107

1Z: Cilia- and flagella-associated protein 107

2: Dynein regulatory complex subunit 2

2A: Protein Flattop

2B: Protein Flattop

2C: Protein Flattop

2D: Protein Flattop

2E: Protein Flattop

2F: Protein Flattop

2G: Protein Flattop

2I: Cilia- and flagella-associated protein 141

2J: Cilia- and flagella-associated protein 141

2L: Cilia- and flagella-associated protein 161

2M: Cilia- and flagella-associated protein 161

2N: Cilia- and flagella-associated protein 161

2O: Cilia- and flagella-associated protein 161

2Q: Cilia- and flagella- associated protein 210

2R: Cilia- and flagella- associated protein 210

2S: Cilia- and flagella- associated protein 210

2U: Cilia- and flagella-associated protein 276

2V: Cilia- and flagella-associated protein 276

2W: Cilia- and flagella-associated protein 276

2X: Cilia- and flagella-associated protein 276

2Y: Cilia- and flagella-associated protein 276

2Z: Cilia- and flagella-associated protein 276

3: Dynein regulatory complex subunit 3

3A: EF-hand domain-containing protein 1

3B: EF-hand domain-containing protein 1

3C: EF-hand domain-containing protein 1

3D: EF-hand domain-containing protein 1

3E: EF-hand domain-containing protein 1

3F: EF-hand domain-containing protein 1

3G: EF-hand domain-containing family member C2

3H: EF-hand domain-containing family member C2

3I: EF-hand domain-containing family member C2

3J: EF-hand domain-containing family member C2

3K: EF-hand domain-containing family member C2

3L: EF-hand domain-containing family member C2

3M: EF-hand domain-containing family member C2

3O: Enkurin

3P: Enkurin

3Q: Enkurin

3R: Enkurin

3S: Enkurin

3T: Enkurin

3U: Enkurin

3W: Meiosis-specific nuclear structural protein 1

3X: Meiosis-specific nuclear structural protein 1

3Y: Meiosis-specific nuclear structural protein 1

4: Dynein regulatory complex subunit 4

4A: Protein FAM166B

4B: Protein FAM166B

4C: Protein FAM166B

4D: Protein FAM166B

4E: Protein FAM166B

4F: Protein FAM166B

4G: Protein FAM166B

4H: Protein FAM166B

4I: Protein FAM166B

4J: Protein FAM166B

4K: Protein FAM166B

4L: Protein FAM166B

4M: Protein FAM166B

4O: Nucleoside diphosphate kinase 7

4P: Nucleoside diphosphate kinase 7

4Q: Nucleoside diphosphate kinase 7

4R: Nucleoside diphosphate kinase 7

4S: Piercer of microtubule wall 1 protein

4T: Piercer of microtubule wall 1 protein

4U: Piercer of microtubule wall 2 protein

4V: Piercer of microtubule wall 2 protein

4W: Piercer of microtubule wall 2 protein

5: Dynein regulatory complex subunit 5

5A: RIB43A-like with coiled-coils protein 2

5B: RIB43A-like with coiled-coils protein 2

5C: RIB43A-like with coiled-coils protein 2

5D: RIB43A-like with coiled-coils protein 1

5E: RIB43A-like with coiled-coils protein 1

5F: RIB43A-like with coiled-coils protein 1

5H: Sperm-associated antigen 8

5I: Sperm-associated antigen 8

5J: Sperm-associated antigen 8

5L: Uncharacterized protein CFAP68

5M: Uncharacterized protein CFAP68

5O: Cilia- and flagella-associated protein 77

5P: Cilia- and flagella-associated protein 77

5Q: Cilia- and flagella-associated protein 77

5R: Cilia- and flagella-associated protein 77

5S: Cilia- and flagella-associated protein 77

5T: Cilia- and flagella-associated protein 77

5U: Cilia- and flagella-associated protein 77

5V: Cilia- and flagella-associated protein 77

5X: Uncharacterized protein CFAP90

5Y: Uncharacterized protein CFAP90

6A: Sperm acrosome-associated protein 9

6C: Sperm acrosome-associated protein 9

6E: Sperm acrosome-associated protein 9

6G: Sperm acrosome-associated protein 9

6I: Sperm acrosome-associated protein 9

6K: Sperm acrosome-associated protein 9

6L: Sperm acrosome-associated protein 9

6N: Sperm acrosome-associated protein 9

6O: Sperm acrosome-associated protein 9

6Q: Sperm acrosome-associated protein 9

6R: Sperm acrosome-associated protein 9

6T: Sperm acrosome-associated protein 9

6V: Sperm acrosome-associated protein 9

7: Dynein regulatory complex subunit 7

7A: Protein FAM166C

7B: Protein FAM166C

7C: Protein FAM166C

7D: Protein FAM166C

7E: Protein FAM166C

7F: Protein FAM166C

7G: Protein FAM166C

7I: Cilia- and flagella-associated protein 144

7J: Cilia- and flagella-associated protein 144

8: Dynein regulatory complex protein 8

8A: Tektin-1

8B: Tektin-1

8C: Tektin-1

8D: Tektin-1

8E: Tektin-1

8F: Tektin-1

8G: Tektin-1

8H: Tektin-1

8J: Tektin-2

8K: Tektin-2

8L: Tektin-2

8M: Tektin-2

8N: Tektin-2

8O: Tektin-2

8P: Tektin-2

8Q: Tektin-2

9: Dynein regulatory complex protein 9

A: Radial spoke head 1 homolog

AZ: Tubulin alpha-1A chain

AB: Tubulin beta-4B chain

AC: Tubulin alpha-1A chain

AD: Tubulin beta-4B chain

AE: Tubulin alpha-1A chain

AF: Tubulin beta-4B chain

AG: Tubulin alpha-1A chain

AH: Tubulin beta-4B chain

AI: Tubulin alpha-1A chain

AJ: Tubulin beta-4B chain

AK: Tubulin alpha-1A chain

AL: Tubulin beta-4B chain

AM: Tubulin alpha-1A chain

AN: Tubulin beta-4B chain

AO: Tubulin alpha-1A chain

AP: Tubulin beta-4B chain

AQ: Tubulin alpha-1A chain

AR: Tubulin beta-4B chain

AS: Tubulin alpha-1A chain

AT: Tubulin beta-4B chain

AU: Tubulin alpha-1A chain

AV: Tubulin beta-4B chain

AW: Tubulin alpha-1A chain

AX: Tubulin beta-4B chain

AY: Tubulin alpha-1A chain

B: Radial spoke head 1 homolog

BA: Tubulin alpha-1A chain

BB: Tubulin beta-4B chain

BC: Tubulin alpha-1A chain

BD: Tubulin beta-4B chain

BE: Tubulin alpha-1A chain

BF: Tubulin beta-4B chain

BG: Tubulin alpha-1A chain

BH: Tubulin beta-4B chain

BI: Tubulin alpha-1A chain

BJ: Tubulin beta-4B chain

BK: Tubulin alpha-1A chain

BL: Tubulin beta-4B chain

BM: Tubulin alpha-1A chain

BN: Tubulin beta-4B chain

BO: Tubulin alpha-1A chain

BP: Tubulin beta-4B chain

BQ: Tubulin alpha-1A chain

BR: Tubulin beta-4B chain

BS: Tubulin alpha-1A chain

BT: Tubulin beta-4B chain

BU: Tubulin alpha-1A chain

BV: Tubulin beta-4B chain

BW: Tubulin alpha-1A chain

BX: Tubulin beta-4B chain

BY: Tubulin alpha-1A chain

C: Radial spoke head 1 homolog

CB: Tubulin beta-4B chain

CC: Tubulin alpha-1A chain

CD: Tubulin beta-4B chain

CE: Tubulin alpha-1A chain

CF: Tubulin beta-4B chain

CG: Tubulin alpha-1A chain

CH: Tubulin beta-4B chain

CI: Tubulin alpha-1A chain

CJ: Tubulin beta-4B chain

CK: Tubulin alpha-1A chain

CL: Tubulin beta-4B chain

CM: Tubulin alpha-1A chain

CN: Tubulin beta-4B chain

CO: Tubulin alpha-1A chain

CP: Tubulin beta-4B chain

CQ: Tubulin alpha-1A chain

CR: Tubulin beta-4B chain

CS: Tubulin alpha-1A chain

CT: Tubulin beta-4B chain

CU: Tubulin alpha-1A chain

CV: Tubulin beta-4B chain

CW: Tubulin alpha-1A chain

CX: Tubulin beta-4B chain

CY: Tubulin alpha-1A chain

CZ: Tubulin beta-4B chain

D: Radial spoke head 1 homolog

DB: Tubulin beta-4B chain

DC: Tubulin alpha-1A chain

DD: Tubulin beta-4B chain

DE: Tubulin alpha-1A chain

DF: Tubulin beta-4B chain

DG: Tubulin alpha-1A chain

DH: Tubulin beta-4B chain

DI: Tubulin alpha-1A chain

DJ: Tubulin beta-4B chain

DK: Tubulin alpha-1A chain

DL: Tubulin beta-4B chain

DM: Tubulin alpha-1A chain

DN: Tubulin beta-4B chain

DO: Tubulin alpha-1A chain

DP: Tubulin beta-4B chain

DQ: Tubulin alpha-1A chain

DR: Tubulin beta-4B chain

DS: Tubulin alpha-1A chain

DT: Tubulin beta-4B chain

DU: Tubulin alpha-1A chain

DV: Tubulin beta-4B chain

DW: Tubulin alpha-1A chain

DX: Tubulin beta-4B chain

DY: Tubulin alpha-1A chain

DZ: Tubulin beta-4B chain

E: Radial spoke head protein 3 homolog

EB: Tubulin beta-4B chain

EC: Tubulin alpha-1A chain

ED: Tubulin beta-4B chain

EE: Tubulin alpha-1A chain

EF: Tubulin beta-4B chain

EG: Tubulin alpha-1A chain

EH: Tubulin beta-4B chain

EI: Tubulin alpha-1A chain

EJ: Tubulin beta-4B chain

EK: Tubulin alpha-1A chain

EL: Tubulin beta-4B chain

EM: Tubulin alpha-1A chain

EN: Tubulin beta-4B chain

EO: Tubulin alpha-1A chain

EP: Tubulin beta-4B chain

EQ: Tubulin alpha-1A chain

ER: Tubulin beta-4B chain

ES: Tubulin alpha-1A chain

ET: Tubulin beta-4B chain

EU: Tubulin alpha-1A chain

EV: Tubulin beta-4B chain

EW: Tubulin alpha-1A chain

EX: Tubulin beta-4B chain

EY: Tubulin alpha-1A chain

EZ: Tubulin beta-4B chain

F: Radial spoke head protein 3 homolog

FB: Tubulin beta-4B chain

FC: Tubulin alpha-1A chain

FD: Tubulin beta-4B chain

FE: Tubulin alpha-1A chain

FF: Tubulin beta-4B chain

FG: Tubulin alpha-1A chain

FH: Tubulin beta-4B chain

FI: Tubulin alpha-1A chain

FJ: Tubulin beta-4B chain

FK: Tubulin alpha-1A chain

FL: Tubulin beta-4B chain

FM: Tubulin alpha-1A chain

FN: Tubulin beta-4B chain

FO: Tubulin alpha-1A chain

FP: Tubulin beta-4B chain

FQ: Tubulin alpha-1A chain

FR: Tubulin beta-4B chain

FS: Tubulin alpha-1A chain

FT: Tubulin beta-4B chain

FU: Tubulin alpha-1A chain

FV: Tubulin beta-4B chain

FW: Tubulin alpha-1A chain

FX: Tubulin beta-4B chain

FY: Tubulin alpha-1A chain

FZ: Tubulin beta-4B chain

G: Radial spoke head protein 4 homolog A

GB: Tubulin beta-4B chain

GC: Tubulin alpha-1A chain

GD: Tubulin beta-4B chain

GE: Tubulin alpha-1A chain

GF: Tubulin beta-4B chain

GG: Tubulin alpha-1A chain

GH: Tubulin beta-4B chain

GI: Tubulin alpha-1A chain

GJ: Tubulin beta-4B chain

GK: Tubulin alpha-1A chain

GL: Tubulin beta-4B chain

GM: Tubulin alpha-1A chain

GN: Tubulin beta-4B chain

GO: Tubulin alpha-1A chain

GP: Tubulin beta-4B chain

GQ: Tubulin alpha-1A chain

GR: Tubulin beta-4B chain

GS: Tubulin alpha-1A chain

GT: Tubulin beta-4B chain

GU: Tubulin alpha-1A chain

GV: Tubulin beta-4B chain

GW: Tubulin alpha-1A chain

GX: Tubulin beta-4B chain

GY: Tubulin alpha-1A chain

GZ: Tubulin beta-4B chain

H: Radial spoke head protein 4 homolog A

HA: Tubulin alpha-1A chain

HB: Tubulin beta-4B chain

HC: Tubulin alpha-1A chain

HD: Tubulin beta-4B chain

HE: Tubulin alpha-1A chain

HF: Tubulin beta-4B chain

HG: Tubulin alpha-1A chain

HH: Tubulin beta-4B chain

HI: Tubulin alpha-1A chain

HJ: Tubulin beta-4B chain

HK: Tubulin alpha-1A chain

HL: Tubulin beta-4B chain

HM: Tubulin alpha-1A chain

HN: Tubulin beta-4B chain

HO: Tubulin alpha-1A chain

HP: Tubulin beta-4B chain

HQ: Tubulin alpha-1A chain

HR: Tubulin beta-4B chain

HS: Tubulin alpha-1A chain

HT: Tubulin beta-4B chain

HU: Tubulin alpha-1A chain

HV: Tubulin beta-4B chain

HW: Tubulin alpha-1A chain

HX: Tubulin beta-4B chain

HY: Tubulin alpha-1A chain

HZ: Tubulin beta-4B chain

I: Radial spoke head protein 4 homolog A

IA: Tubulin alpha-1A chain

IB: Tubulin beta-4B chain

IC: Tubulin alpha-1A chain

ID: Tubulin beta-4B chain

IE: Tubulin alpha-1A chain

IF: Tubulin beta-4B chain

IG: Tubulin alpha-1A chain

IH: Tubulin beta-4B chain

II: Tubulin alpha-1A chain

IJ: Tubulin beta-4B chain

IK: Tubulin alpha-1A chain

IL: Tubulin beta-4B chain

IM: Tubulin alpha-1A chain

IN: Tubulin beta-4B chain

IO: Tubulin alpha-1A chain

IP: Tubulin beta-4B chain

IQ: Tubulin alpha-1A chain

IR: Tubulin beta-4B chain

IS: Tubulin alpha-1A chain

IT: Tubulin beta-4B chain

IU: Tubulin alpha-1A chain

IV: Tubulin beta-4B chain

IW: Tubulin alpha-1A chain

IX: Tubulin beta-4B chain

IY: Tubulin alpha-1A chain

J: Radial spoke head protein 4 homolog A

JB: Tubulin beta-4B chain

JC: Tubulin alpha-1A chain

JD: Tubulin beta-4B chain

JE: Tubulin alpha-1A chain

JF: Tubulin beta-4B chain

JG: Tubulin alpha-1A chain

JH: Tubulin beta-4B chain

JI: Tubulin alpha-1A chain

JJ: Tubulin beta-4B chain

JK: Tubulin alpha-1A chain

JL: Tubulin beta-4B chain

JM: Tubulin alpha-1A chain

JN: Tubulin beta-4B chain

JO: Tubulin alpha-1A chain

JP: Tubulin beta-4B chain

JQ: Tubulin alpha-1A chain

JR: Tubulin beta-4B chain

JS: Tubulin alpha-1A chain

JT: Tubulin beta-4B chain

JU: Tubulin alpha-1A chain

JV: Tubulin beta-4B chain

JW: Tubulin alpha-1A chain

JX: Tubulin beta-4B chain

JY: Tubulin alpha-1A chain

JZ: Tubulin beta-4B chain

K: Sperm surface protein Sp17

KB: Tubulin beta-4B chain

KC: Tubulin alpha-1A chain

KD: Tubulin beta-4B chain

KE: Tubulin alpha-1A chain

KF: Tubulin beta-4B chain

KG: Tubulin alpha-1A chain

KH: Tubulin beta-4B chain

KI: Tubulin alpha-1A chain

KJ: Tubulin beta-4B chain

KK: Tubulin alpha-1A chain

KL: Tubulin beta-4B chain

KM: Tubulin alpha-1A chain

KN: Tubulin beta-4B chain

KO: Tubulin alpha-1A chain

KP: Tubulin beta-4B chain

KQ: Tubulin alpha-1A chain

KR: Tubulin beta-4B chain

KS: Tubulin alpha-1A chain

KT: Tubulin beta-4B chain

KU: Tubulin alpha-1A chain

KV: Tubulin beta-4B chain

KW: Tubulin alpha-1A chain

KX: Tubulin beta-4B chain

KY: Tubulin alpha-1A chain

KZ: Tubulin beta-4B chain

L: Sperm surface protein Sp17

LB: Tubulin beta-4B chain

LC: Tubulin alpha-1A chain

LD: Tubulin beta-4B chain

LE: Tubulin alpha-1A chain

LF: Tubulin beta-4B chain

LG: Tubulin alpha-1A chain

LH: Tubulin beta-4B chain

LI: Tubulin alpha-1A chain

LJ: Tubulin beta-4B chain

LK: Tubulin alpha-1A chain

LL: Tubulin beta-4B chain

LM: Tubulin alpha-1A chain

LN: Tubulin beta-4B chain

LO: Tubulin alpha-1A chain

LP: Tubulin beta-4B chain

LQ: Tubulin alpha-1A chain

LR: Tubulin beta-4B chain

LS: Tubulin alpha-1A chain

LT: Tubulin beta-4B chain

LU: Tubulin alpha-1A chain

LV: Tubulin beta-4B chain

LW: Tubulin alpha-1A chain

LX: Tubulin beta-4B chain

LY: Tubulin alpha-1A chain

LZ: Tubulin beta-4B chain

M: Radial spoke head protein 9 homolog

MA: Tubulin alpha-1A chain

MC: Tubulin alpha-1A chain

MD: Tubulin beta-4B chain

ME: Tubulin alpha-1A chain

MF: Tubulin beta-4B chain

MG: Tubulin alpha-1A chain

MH: Tubulin beta-4B chain

MI: Tubulin alpha-1A chain

MJ: Tubulin beta-4B chain

MK: Tubulin alpha-1A chain

ML: Tubulin beta-4B chain

MM: Tubulin alpha-1A chain

MN: Tubulin beta-4B chain

MO: Tubulin alpha-1A chain

MP: Tubulin beta-4B chain

MQ: Tubulin alpha-1A chain

MR: Tubulin beta-4B chain

MS: Tubulin alpha-1A chain

MT: Tubulin beta-4B chain

MU: Tubulin alpha-1A chain

MV: Tubulin beta-4B chain

MW: Tubulin alpha-1A chain

MX: Tubulin beta-4B chain

MY: Tubulin alpha-1A chain

MZ: Tubulin beta-4B chain

N: Radial spoke head protein 9 homolog

NA: Tubulin alpha-1A chain

NB: Tubulin beta-4B chain

NC: Tubulin alpha-1A chain

ND: Tubulin beta-4B chain

NE: Tubulin alpha-1A chain

NF: Tubulin beta-4B chain

NG: Tubulin alpha-1A chain

NH: Tubulin beta-4B chain

NI: Tubulin alpha-1A chain

NJ: Tubulin beta-4B chain

NK: Tubulin alpha-1A chain

NL: Tubulin beta-4B chain

NM: Tubulin alpha-1A chain

NN: Tubulin beta-4B chain

NO: Tubulin alpha-1A chain

NP: Tubulin beta-4B chain

NQ: Tubulin alpha-1A chain

NR: Tubulin beta-4B chain

NS: Tubulin alpha-1A chain

NT: Tubulin beta-4B chain

NU: Tubulin alpha-1A chain

NV: Tubulin beta-4B chain

NW: Tubulin alpha-1A chain

NX: Tubulin beta-4B chain

NZ: Tubulin beta-4B chain

O: Radial spoke head protein 9 homolog

OA: Tubulin alpha-1A chain

OB: Tubulin beta-4B chain

OC: Tubulin alpha-1A chain

OD: Tubulin beta-4B chain

OE: Tubulin alpha-1A chain

OF: Tubulin beta-4B chain

OG: Tubulin alpha-1A chain

OH: Tubulin beta-4B chain

OI: Tubulin alpha-1A chain

OJ: Tubulin beta-4B chain

OK: Tubulin alpha-1A chain

OL: Tubulin beta-4B chain

OM: Tubulin alpha-1A chain

ON: Tubulin beta-4B chain

OO: Tubulin alpha-1A chain

OP: Tubulin beta-4B chain

OQ: Tubulin alpha-1A chain

OR: Tubulin beta-4B chain

OS: Tubulin alpha-1A chain

OT: Tubulin beta-4B chain

OU: Tubulin alpha-1A chain

OV: Tubulin beta-4B chain

OW: Tubulin alpha-1A chain

OX: Tubulin beta-4B chain

OY: Tubulin alpha-1A chain

P: Radial spoke head protein 9 homolog

PA: Tubulin alpha-1A chain

PB: Tubulin beta-4B chain

PC: Tubulin alpha-1A chain

PD: Tubulin beta-4B chain

PE: Tubulin alpha-1A chain

PF: Tubulin beta-4B chain

PG: Tubulin alpha-1A chain

PH: Tubulin beta-4B chain

PI: Tubulin alpha-1A chain

PJ: Tubulin beta-4B chain

PK: Tubulin alpha-1A chain

PL: Tubulin beta-4B chain

PM: Tubulin alpha-1A chain

PN: Tubulin beta-4B chain

PO: Tubulin alpha-1A chain

PP: Tubulin beta-4B chain

PQ: Tubulin alpha-1A chain

PR: Tubulin beta-4B chain

PS: Tubulin alpha-1A chain

PT: Tubulin beta-4B chain

PU: Tubulin alpha-1A chain

PV: Tubulin beta-4B chain

PW: Tubulin alpha-1A chain

PX: Tubulin beta-4B chain

PY: Tubulin alpha-1A chain

Q: Ropporin-1-like protein

QA: Tubulin alpha-1A chain

QB: Tubulin beta-4B chain

QC: Tubulin alpha-1A chain

QD: Tubulin beta-4B chain

QE: Tubulin alpha-1A chain

QF: Tubulin beta-4B chain

QG: Tubulin alpha-1A chain

QH: Tubulin beta-4B chain

QI: Tubulin alpha-1A chain

QJ: Tubulin beta-4B chain

QK: Tubulin alpha-1A chain

QL: Tubulin beta-4B chain

QM: Tubulin alpha-1A chain

QN: Tubulin beta-4B chain

QO: Tubulin alpha-1A chain

QP: Tubulin beta-4B chain

QQ: Tubulin alpha-1A chain

QR: Tubulin beta-4B chain

QS: Tubulin alpha-1A chain

QT: Tubulin beta-4B chain

QU: Tubulin alpha-1A chain

QV: Tubulin beta-4B chain

QW: Tubulin alpha-1A chain

QX: Tubulin beta-4B chain

QY: Tubulin alpha-1A chain

R: Ropporin-1-like protein

RA: Tubulin alpha-1A chain

RB: Tubulin beta-4B chain

RC: Tubulin alpha-1A chain

RD: Tubulin beta-4B chain

RE: Tubulin alpha-1A chain

RF: Tubulin beta-4B chain

RG: Tubulin alpha-1A chain

RH: Tubulin beta-4B chain

RI: Tubulin alpha-1A chain

RJ: Tubulin beta-4B chain

RK: Tubulin alpha-1A chain

RL: Tubulin beta-4B chain

RM: Tubulin alpha-1A chain

RN: Tubulin beta-4B chain

RO: Tubulin alpha-1A chain

RP: Tubulin beta-4B chain

RQ: Tubulin alpha-1A chain

RR: Tubulin beta-4B chain

RS: Tubulin alpha-1A chain

RT: Tubulin beta-4B chain

RU: Tubulin alpha-1A chain

RV: Tubulin beta-4B chain

RW: Tubulin alpha-1A chain

RX: Tubulin beta-4B chain

RY: Tubulin alpha-1A chain

S: Probable inactive peptidyl-prolyl cis-trans isomerase-like 6

SB: Tubulin beta-4B chain

SC: Tubulin alpha-1A chain

SD: Tubulin beta-4B chain

SE: Tubulin alpha-1A chain

SF: Tubulin beta-4B chain

SG: Tubulin alpha-1A chain

SH: Tubulin beta-4B chain

SI: Tubulin alpha-1A chain

SJ: Tubulin beta-4B chain

SK: Tubulin alpha-1A chain

SL: Tubulin beta-4B chain

SM: Tubulin alpha-1A chain

SN: Tubulin beta-4B chain

SO: Tubulin alpha-1A chain

SP: Tubulin beta-4B chain

SQ: Tubulin alpha-1A chain

SR: Tubulin beta-4B chain

SS: Tubulin alpha-1A chain

ST: Tubulin beta-4B chain

SU: Tubulin alpha-1A chain

SV: Tubulin beta-4B chain

SW: Tubulin alpha-1A chain

SX: Tubulin beta-4B chain

SY: Tubulin alpha-1A chain

T: Radial spoke head 14 homolog

TB: Tubulin beta-4B chain

TC: Tubulin alpha-1A chain

TD: Tubulin beta-4B chain

TE: Tubulin alpha-1A chain

TF: Tubulin beta-4B chain

TG: Tubulin alpha-1A chain

TH: Tubulin beta-4B chain

TI: Tubulin alpha-1A chain

TJ: Tubulin beta-4B chain

TK: Tubulin alpha-1A chain

TL: Tubulin beta-4B chain

TM: Tubulin alpha-1A chain

TN: Tubulin beta-4B chain

TO: Tubulin alpha-1A chain

TP: Tubulin beta-4B chain

TQ: Tubulin alpha-1A chain

TR: Tubulin beta-4B chain

TS: Tubulin alpha-1A chain

TT: Tubulin beta-4B chain

TU: Tubulin alpha-1A chain

TV: Tubulin beta-4B chain

TW: Tubulin alpha-1A chain

TX: Tubulin beta-4B chain

TY: Tubulin alpha-1A chain

TZ: Tubulin beta-4B chain

U: DnaJ homolog subfamily B member 13

UB: Tubulin beta-4B chain

UC: Tubulin alpha-1A chain

UD: Tubulin beta-4B chain

UE: Tubulin alpha-1A chain

UF: Tubulin beta-4B chain

UG: Tubulin alpha-1A chain

UH: Tubulin beta-4B chain

UI: Tubulin alpha-1A chain

UJ: Tubulin beta-4B chain

UK: Tubulin alpha-1A chain

UL: Tubulin beta-4B chain

UM: Tubulin alpha-1A chain

UN: Tubulin beta-4B chain

UO: Tubulin alpha-1A chain

UP: Tubulin beta-4B chain

UQ: Tubulin alpha-1A chain

UR: Tubulin beta-4B chain

US: Tubulin alpha-1A chain

UT: Tubulin beta-4B chain

UU: Tubulin alpha-1A chain

UV: Tubulin beta-4B chain

UW: Tubulin alpha-1A chain

UX: Tubulin beta-4B chain

UY: Tubulin alpha-1A chain

UZ: Tubulin beta-4B chain

V: DnaJ homolog subfamily B member 13

VB: Tubulin beta-4B chain

VC: Tubulin alpha-1A chain

VD: Tubulin beta-4B chain

VE: Tubulin alpha-1A chain

VF: Tubulin beta-4B chain

VG: Tubulin alpha-1A chain

VH: Tubulin beta-4B chain

VI: Tubulin alpha-1A chain

VJ: Tubulin beta-4B chain

VK: Tubulin alpha-1A chain

VL: Tubulin beta-4B chain

VM: Tubulin alpha-1A chain

VN: Tubulin beta-4B chain

VO: Tubulin alpha-1A chain

VP: Tubulin beta-4B chain

VQ: Tubulin alpha-1A chain

VR: Tubulin beta-4B chain

VS: Tubulin alpha-1A chain

VT: Tubulin beta-4B chain

VU: Tubulin alpha-1A chain

VV: Tubulin beta-4B chain

VW: Tubulin alpha-1A chain

VX: Tubulin beta-4B chain

VY: Tubulin alpha-1A chain

VZ: Tubulin beta-4B chain

W: Calmodulin-1

WA: Tubulin alpha-1A chain

WB: Tubulin beta-4B chain

WC: Tubulin alpha-1A chain

WD: Tubulin beta-4B chain

WE: Tubulin alpha-1A chain

WF: Tubulin beta-4B chain

WG: Tubulin alpha-1A chain

WH: Tubulin beta-4B chain

WI: Tubulin alpha-1A chain

WJ: Tubulin beta-4B chain

WK: Tubulin alpha-1A chain

WL: Tubulin beta-4B chain

WM: Tubulin alpha-1A chain

WN: Tubulin beta-4B chain

WO: Tubulin alpha-1A chain

WP: Tubulin beta-4B chain

WQ: Tubulin alpha-1A chain

WR: Tubulin beta-4B chain

WS: Tubulin alpha-1A chain

WT: Tubulin beta-4B chain

WU: Tubulin alpha-1A chain

WV: Tubulin beta-4B chain

WW: Tubulin alpha-1A chain

WX: Tubulin beta-4B chain

X: Nucleoside diphosphate kinase homolog 5

XA: Cilia- and flagella-associated protein 20

XB: Cilia- and flagella-associated protein 20

XC: Cilia- and flagella-associated protein 20

XD: Cilia- and flagella-associated protein 20

XE: Cilia- and flagella-associated protein 20

XF: Cilia- and flagella-associated protein 20

XG: Cilia- and flagella-associated protein 20

XH: Cilia- and flagella-associated protein 20

XI: Cilia- and flagella-associated protein 20

XJ: Cilia- and flagella-associated protein 20

XK: Cilia- and flagella-associated protein 20

XL: Cilia- and flagella-associated protein 20

XM: Cilia- and flagella-associated protein 20

Y: Nucleoside diphosphate kinase homolog 5

YB: Parkin coregulated gene protein

YC: Parkin coregulated gene protein

YD: Parkin coregulated gene protein

YF: Parkin coregulated gene protein

YG: Parkin coregulated gene protein

YH: Parkin coregulated gene protein

YI: Parkin coregulated gene protein

YJ: Parkin coregulated gene protein

YK: Parkin coregulated gene protein

YL: Parkin coregulated gene protein

YM: Parkin coregulated gene protein

Z: Cytochrome b5 domain-containing protein 1

a: Radial spoke head 1 homolog

a0: Unknown

a1: DYNLL1

a2: DYNLL1

a3: DYNLL1

a4: DYNLL1

a5: DYNLL1

a6: DYNLL1

a7: DYNLL1

a8: DYNLL1

a9: DYNLL1

b: Radial spoke head 1 homolog

b0: DYNLL1

b1: DYNLL1

b2: DYNLL1

b3: DYNLL1

b4: DYNLL1

b5: DYNLL1

b6: DYNLL1

b7: DYNLL1

b8: DYNLL1

b9: DYNLL1

c: Radial spoke head 1 homolog

c0: DYNLL1

c1: MORN repeat-containing protein 3

c2: IQ and ubiquitin-like domain-containing protein

c3: DPY30 domain-containing protein 2

c4: DPY30 domain-containing protein 2

c5: DPY30 domain-containing protein 2

c6: DPY30 domain-containing protein 2

d: Radial spoke head 1 homolog

d0: Cilia- and flagella-associated protein 61

d1: Cilia- and flagella-associated protein 91

d2: Cilia- and flagella-associated protein 251

d3: Tetratricopeptide repeat protein 29

d4: Zinc finger MYND domain-containing protein 12

d5: ACTA2

d6: ACTA2

d7: ACTA2

d8: ACTA2

d9: ACTA2

e: Radial spoke head protein 3 homolog

e0: Axonemal dynein light intermediate polypeptide 1

e1: Axonemal dynein light intermediate polypeptide 1

e2: Axonemal dynein light intermediate polypeptide 1

e3: Axonemal dynein light intermediate polypeptide 1

e4: Axonemal dynein light intermediate polypeptide 1

e5: Axonemal dynein light intermediate polypeptide 1

e6: Axonemal dynein light intermediate polypeptide 1

e7: Axonemal dynein light intermediate polypeptide 1

e8: Axonemal dynein light intermediate polypeptide 1

e9: Axonemal dynein light intermediate polypeptide 1

f: Radial spoke head protein 3 homolog

f1: ACTA2

f2: Centrin-2

g: Radial spoke head protein 4 homolog A

g1: Dynein axonemal heavy chain 12

g2: Dynein axonemal heavy chain 7

g3: Dynein axonemal heavy chain 3

g4: Dynein axonemal heavy chain 7

g5: Dynein axonemal heavy chain 6

g6: Dynein axonemal heavy chain 1

h: Radial spoke head protein 4 homolog A

h1: Coiled-coil domain-containing protein 39

h2: Coiled-coil domain-containing protein 39

h3: Coiled-coil domain-containing protein 40

h4: Coiled-coil domain-containing protein 40

i: Radial spoke head protein 4 homolog A

i1: Coiled-coil domain-containing protein 96

i2: Coiled-coil domain-containing protein 113

i3: Cilia- and flagella-associated protein 299

i4: Cilia- and flagella-associated protein 57

i5: Cilia- and flagella-associated protein 57

i6: Cilia- and flagella-associated protein 43

i7: Cilia- and flagella-associated protein 44

i8: Cilia- and flagella-associated protein 43

i9: Cilia- and flagella-associated protein 44

j: Radial spoke head protein 4 homolog A

j1: Cilia- and flagella-associated protein 58

j2: Cilia- and flagella-associated protein 58

j3: Cilia- and flagella-associated protein 73

j4: Cilia- and flagella-associated protein 100

k: Sperm surface protein Sp17

k1: Dynein axonemal intermediate chain 3

k2: Dynein axonemal intermediate chain 4

k3: Dynein axonemal intermediate chain 7

k4: Dynein light chain roadblock-type 1

k5: Dynein light chain roadblock-type 2

k8: Dynein axonemal heavy chain 10

k9: Dynein axonemal heavy chain 2

l: Sperm surface protein Sp17

l1: DYNLL1

l2: DYNLL1

l3: DYNLL1

l4: DYNLL1

l5: DYNLL1

l6: DYNLL1

l7: Dynein light chain Tctex-type protein 2B

l8: Dynein light chain Tctex-type 1

m: Radial spoke head protein 9 homolog

m0: Calaxin

m1: Dynein axonemal intermediate chain 1

m2: Dynein axonemal intermediate chain 2

m3: Dynein axonemal light chain 1

m4: Dynein light chain roadblock-type 1

m5: Dynein light chain roadblock-type 2

m6: Outer dynein arm-docking complex subunit 1

m7: Outer dynein arm-docking complex subunit 2

m8: Outer dynein arm-docking complex subunit 3

m9: Outer dynein arm-docking complex subunit 4

n: Radial spoke head protein 9 homolog

n0: Dynein axonemal heavy chain 5

n1: DYNLL1

n2: DYNLL1

n3: DYNLL1

n4: DYNLL1

n5: DYNLL1

n6: DYNLL1

n7: Dynein light chain Tctex-type protein 2B

n8: Dynein light chain Tctex-type 1

n9: Dynein axonemal heavy chain 9

o: Radial spoke head protein 9 homolog

o1: Dynein axonemal intermediate chain 1

o2: Dynein axonemal intermediate chain 2

o3: Dynein axonemal light chain 1

o4: Dynein light chain roadblock-type 1

o5: Dynein light chain roadblock-type 2

o6: Outer dynein arm-docking complex subunit 1

o8: Outer dynein arm-docking complex subunit 3

p: Radial spoke head protein 9 homolog

p0: Dynein axonemal heavy chain 5

p1: DYNLL1

p2: DYNLL1

p3: DYNLL1

p4: DYNLL1

p5: DYNLL1

p6: DYNLL1

p7: Dynein light chain Tctex-type protein 2B

p8: Dynein light chain Tctex-type 1

p9: Dynein axonemal heavy chain 9

q: Ropporin-1-like protein

q0: Calaxin

q1: Dynein axonemal intermediate chain 1

q2: Dynein axonemal intermediate chain 2

q3: Dynein axonemal light chain 1

q4: Dynein light chain roadblock-type 1

q5: Dynein light chain roadblock-type 2

q6: Outer dynein arm-docking complex subunit 1

q7: Outer dynein arm-docking complex subunit 2

q8: Outer dynein arm-docking complex subunit 3

q9: Outer dynein arm-docking complex subunit 4

r: Ropporin-1-like protein

r0: Dynein axonemal heavy chain 5

r1: DYNLL1

r2: DYNLL1

r3: DYNLL1

r4: DYNLL1

r5: DYNLL1

r6: DYNLL1

r7: Dynein light chain Tctex-type protein 2B

r8: Dynein light chain Tctex-type 1

r9: Dynein axonemal heavy chain 9

s: Probable inactive peptidyl-prolyl cis-trans isomerase-like 6

s0: Calaxin

s1: Dynein axonemal intermediate chain 1

s2: Dynein axonemal intermediate chain 2

s6: Outer dynein arm-docking complex subunit 1

s7: Outer dynein arm-docking complex subunit 2

s8: Outer dynein arm-docking complex subunit 3

s9: Outer dynein arm-docking complex subunit 4

t: Radial spoke head 14 homolog

t0: Dynein axonemal heavy chain 5

t9: Dynein axonemal heavy chain 9

u: DnaJ homolog subfamily B member 13

u0: Calaxin

u6: Outer dynein arm-docking complex subunit 1

u7: Outer dynein arm-docking complex subunit 2

u8: Outer dynein arm-docking complex subunit 3

u9: Outer dynein arm-docking complex subunit 4

v: DnaJ homolog subfamily B member 13

v1: Thioredoxin domain-containing protein 6

v2: Thioredoxin domain-containing protein 6

v3: Thioredoxin domain-containing protein 6

w: Leucine-rich repeat-containing protein 34

x: Nucleoside diphosphate kinase homolog 5

y: Nucleoside diphosphate kinase homolog 5

z: Cytochrome b5 domain-containing protein 1

hetero molecules

Summary:

• 53.3 MDa, 974 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	53,328,110	1833
Polymers	53,043,992	974
Non-polymers	284,118	859
Water	0	0

1

Summary:

• Idetical with deposited unit
• defined by author
• Evidence: electron microscopy

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555		1

Components

Dynein regulatory complex subunit ... , 5 types, 6 molecules 0 4 2 3 5 7

#1: Protein

0 4 Dynein regulatory complex subunit 4 / Growth arrest-specific protein 11 / GAS-11 / Growth arrest-specific protein 8 / GAS-8

Details:

Mass: 56460.648 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O95995

#12: Protein

2 Dynein regulatory complex subunit 2 / Coiled-coil domain-containing protein 65 / Testis development protein NYD-SP28

Details:

Mass: 57392.855 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IXS2

#18: Protein

3 Dynein regulatory complex subunit 3 / Leucine-rich repeat-containing protein 48

Details:

Mass: 61127.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H069

#27: Protein

5 Dynein regulatory complex subunit 5 / T-complex-associated testis-expressed protein 1 / Tcte-1

Details:

Mass: 55702.281 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5JU00

#35: Protein

7 Dynein regulatory complex subunit 7 / Coiled-coil domain-containing protein 135 / Coiled-coil domain-containing protein lobo homolog

Details:

Mass: 103650.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IY82

Dynein regulatory complex protein ... , 5 types, 8 molecules 1 10 11 12 13 14 8 9

#2: Protein

1 Dynein regulatory complex protein 1 / Coiled-coil domain-containing protein 164

Details:

Mass: 87258.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96MC2

#3: Protein

10 Dynein regulatory complex protein 10 / IQ domain-containing protein D

Details:

Mass: 52449.523 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96DY2

#4: Protein

11 Dynein regulatory complex protein 11 / IQ and AAA domain-containing protein 1

Details:

Mass: 95505.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q86XH1

#5: Protein

12 13 14 8
Dynein regulatory complex protein 8 / EF-hand calcium-binding domain-containing protein 2

Details:

Mass: 29759.723 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5VUJ9

#40: Protein

9 Dynein regulatory complex protein 9 / IQ domain-containing protein G

Details:

Mass: 52000.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H095

EF-hand domain-containing family member ... , 2 types, 10 molecules 1A 1B 1C 3G 3H 3I 3J 3K 3L 3M

#6: Protein

1A 1B 1C EF-hand domain-containing family member B / Cilia- and flagella-associated protein 21

Details:

Mass: 93940.008 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N7U6

#20: Protein

3G 3H 3I 3J 3K 3L 3M
EF-hand domain-containing family member C2

Details:

Mass: 87516.828 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5JST6

Cilia- and flagella-associated protein ... , 21 types, 69 molecules 1D 1E 1F 1G 1H 1I 1J 1L 1M 1N 1O 1P 1Q 1R 1S 1T 1V 1W 1Y 1Z 2I 2J 2L 2M 2N 2O 2U 2V 2W 2X ...

#7: Protein

1D 1E 1F 1G 1H 1I 1J
Cilia- and flagella-associated protein 45 / Coiled-coil domain-containing protein 19 / Nasopharyngeal epithelium-specific protein 1

Details:

Mass: 65858.109 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UL16

#8: Protein

1L 1M 1N 1O 1P 1Q
Cilia- and flagella-associated protein 52 / WD repeat-containing protein 16 / WD40-repeat protein up-regulated in HCC

Details:

Mass: 68379.062 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N1V2

#9: Protein

1R 1S 1T Cilia- and flagella-associated protein 53 / Coiled-coil domain-containing protein 11

Details:

Mass: 61960.844 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96M91

#10: Protein

1V 1W Cilia- and flagella-associated protein 95

Details:

Mass: 26483.875 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5VTT2

#11: Protein

1Y 1Z Cilia- and flagella-associated protein 107

Details:

Mass: 23105.348 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N1D5

#14: Protein

2I 2J Cilia- and flagella-associated protein 141

Details:

Mass: 12151.156 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5VU69

#15: Protein

2L 2M 2N 2O
Cilia- and flagella-associated protein 161

Details:

Mass: 34339.973 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6P656

#17: Protein

2U 2V 2W 2X 2Y 2Z
Cilia- and flagella-associated protein 276

Details:

Mass: 19376.688 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5T5A4

#32: Protein

5O 5P 5Q 5R 5S 5T 5U 5V
Cilia- and flagella-associated protein 77

Details:

Mass: 33037.211 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6ZQR2

#37: Protein

7I 7J Cilia- and flagella-associated protein 144 / Protein FAM183A

Details:

Mass: 16028.189 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A6NL82

#54: Protein

XA XB XC XD XE XF XG XH XI XJ XK XL XM
Cilia- and flagella-associated protein 20 / Basal body up-regulated protein 22 / Transcription factor IIB

Details:

Mass: 22807.469 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y6A4

#62: Protein

d0 Cilia- and flagella-associated protein 61

Details:

Mass: 141511.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8NHU2

#63: Protein

d1 Cilia- and flagella-associated protein 91 / CFAP91 / AMY-1-associating protein expressed in testis 1 / AAT-1 / MYCBP/AMY-1-associated testis-expressed protein 1 / Protein MAATS1

Details:

Mass: 90099.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q7Z4T9

#64: Protein

d2 Cilia- and flagella-associated protein 251 / WD repeat-containing protein 66

Details:

Mass: 130095.477 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TBY9

#79: Protein

i3 Cilia- and flagella-associated protein 299

Details:

Mass: 26905.059 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6V702

#80: Protein

i4 i5 Cilia- and flagella-associated protein 57 / WD repeat-containing protein 65

Details:

Mass: 145159.438 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96MR6

#81: Protein

i6 i8 Cilia- and flagella-associated protein 43 / WD repeat-containing protein 96

Details:

Mass: 192235.375 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8NDM7

#82: Protein

i7 i9 Cilia- and flagella-associated protein 44 / WD repeat-containing protein 52

Details:

Mass: 214164.719 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96MT7

#83: Protein

j1 j2 Cilia- and flagella-associated protein 58 / Coiled-coil domain-containing protein 147

Details:

Mass: 103607.656 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5T655

#84: Protein

j3 Cilia- and flagella-associated protein 73 / Coiled-coil domain-containing protein 42B

Details:

Mass: 35969.023 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A6NFT4

#85: Protein

j4 Cilia- and flagella-associated protein 100 / Coiled-coil domain-containing protein 37

Details:

Mass: 71235.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q494V2

Protein , 34 types, 761 molecules 2A 2B 2C 2D 2E 2F 2G 2Q 2R 2S 3A 3B 3C 3D 3E 3F 3O 3P 3Q 3R 3S 3T 3U 3W 3X 3Y 4A 4B 4C 4D ...

#13: Protein

2A 2B 2C 2D 2E 2F 2G
Protein Flattop / Cilia- and flagella-associated protein 126

Details:

Mass: 19320.871 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5VTH2

#16: Protein

2Q 2R 2S Cilia- and flagella- associated protein 210 / Coiled-coil domain-containing protein 173

Details:

Mass: 66544.008 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q0VFZ6

#19: Protein

3A 3B 3C 3D 3E 3F
EF-hand domain-containing protein 1 / Myoclonin-1

Details:

Mass: 74084.102 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5JVL4

#21: Protein

3O 3P 3Q 3R 3S 3T 3U
Enkurin

Details:

Mass: 29510.348 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TC29

#22: Protein

3W 3X 3Y Meiosis-specific nuclear structural protein 1

Details:

Mass: 60687.531 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8NEH6

#23: Protein

4A 4B 4C 4D 4E 4F 4G 4H 4I 4J 4K 4L 4M
Protein FAM166B

Details:

Mass: 30610.637 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A8MTA8

#30: Protein

5H 5I 5J Sperm-associated antigen 8 / HSD-1 / Sperm membrane protein 1 / SMP-1 / Sperm membrane protein BS-84

Details:

Mass: 51187.598 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q99932

#34: Protein

6A 6C 6E 6G 6I 6K 6L 6N 6O 6Q 6R 6T 6V
Sperm acrosome-associated protein 9

Details:

Mass: 25208.977 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96E40

#36: Protein

7A 7B 7C 7D 7E 7F 7G
Protein FAM166C / Family with sequence similarity 166 member C

Details:

Mass: 23457.842 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A6NJV1

#38: Protein

8A 8B 8C 8D 8E 8F 8G 8H
Tektin-1

Details:

Mass: 48360.113 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q969V4

#39: Protein

8J 8K 8L 8M 8N 8O 8P 8Q
Tektin-2 / Tektin-t / Testicular tektin / Testicular tektin B1-like protein / TEKTB1 / Tektin-B1

Details:

Mass: 49743.176 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UIF3

#42: Protein

AZ AC AE AG AI AK AM AO AQ AS AU AW AY BA BC BE BG BI BK ...
Tubulin alpha-1A chain / Alpha-tubulin 3 / Tubulin B-alpha-1 / Tubulin alpha-3 chain

Details:

Mass: 50188.441 Da / Num. of mol.: 285 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)
References: UniProt: Q71U36, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement

#43: Protein

AB AD AF AH AJ AL AN AP AR AT AV AX BB BD BF BH BJ BL BN ...
Tubulin beta-4B chain / Tubulin beta-2 chain / Tubulin beta-2C chain

Details:

Mass: 49877.824 Da / Num. of mol.: 289 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P68371

#46: Protein

K L k l
Sperm surface protein Sp17 / Cancer/testis antigen 22 / CT22 / Sp17-1 / Sperm autoantigenic protein 17 / Sperm protein 17

Details:

Mass: 17430.072 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q15506

#48: Protein

Q R q r
Ropporin-1-like protein / ROPN1-like protein / AKAP-associated sperm protein

Details:

Mass: 26143.307 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96C74

#49: Protein

S s Probable inactive peptidyl-prolyl cis-trans isomerase-like 6 / PPIase / Cyclophilin-like protein PPIL6 / Rotamase PPIL6

Details:

Mass: 35271.992 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IXY8

#51: Protein

U V u v
DnaJ homolog subfamily B member 13 / Testis and spermatogenesis cell-related protein 6 / Testis spermatocyte apoptosis-related gene 6 protein / Testis spermatogenesis apoptosis-related gene 3 protein / Testis spermatogenesis apoptosis-related gene 6 protein

Details:

Mass: 36169.309 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P59910

#52: Protein

W Calmodulin-1

Details:

Mass: 16852.545 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P0DP23

#55: Protein

YB YC YD YF YG YH YI YJ YK YL YM
Parkin coregulated gene protein / Molecular chaperone/chaperonin-binding protein / PARK2 coregulated gene protein

Details:

Mass: 29352.184 Da / Num. of mol.: 11 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96M98

#56: Protein

Z z Cytochrome b5 domain-containing protein 1

Details:

Mass: 26721.031 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6P9G0

#57: Protein

a0 Unknown

Details:

Mass: 6145.567 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#58: Protein

a1 a2 a3 a4 a5 a6 a7 a8 a9 b0 b1 b2 b3 b4 b5 b6 b7 b8 b9 ...
DYNLL1

Details:

Mass: 10381.899 Da / Num. of mol.: 44 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#59: Protein

c1 MORN repeat-containing protein 3

Details:

Mass: 27625.951 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6PF18

#60: Protein

c2 IQ and ubiquitin-like domain-containing protein

Details:

Mass: 92708.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8NA54

#61: Protein

c3 c4 c5 c6
DPY30 domain-containing protein 2

Details:

Mass: 20614.346 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96IM9

#65: Protein

d3 Tetratricopeptide repeat protein 29 / TPR repeat protein 29 / Protein TBPP2A / Testis development protein NYD-SP14

Details:

Mass: 55157.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8NA56

#66: Protein

d4 Zinc finger MYND domain-containing protein 12

Details:

Mass: 41864.863 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H0C1

#67: Protein

d5 d6 d7 d8 d9 f1
ACTA2

Details:

Mass: 42054.875 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

#68: Protein

e0 e1 e2 e3 e4 e5 e6 e7 e8 e9
Axonemal dynein light intermediate polypeptide 1 / Inner dynein arm light chain / axonemal / hp28

Details:

Mass: 29707.035 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O14645

#69: Protein

f2 Centrin-2 / Caltractin isoform 1

Details:

Mass: 19769.486 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P41208

#95: Protein

m0 q0 s0 u0
Calaxin / EF-hand calcium-binding domain-containing protein 1

Details:

Mass: 24521.121 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9HAE3

#98: Protein

m3 o3 q3 Dynein axonemal light chain 1 / LC1

Details:

Mass: 21563.025 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q4LDG9

#105: Protein

v1 v2 v3 Thioredoxin domain-containing protein 6 / Thioredoxin-like protein 2 / Txl-2

Details:

Mass: 36902.664 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q86XW9

#106: Protein

w Leucine-rich repeat-containing protein 34

Details:

Mass: 51331.484 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IZ02

Nucleoside diphosphate kinase ... , 2 types, 8 molecules 4O 4P 4Q 4R X Y x y

#24: Protein

4O 4P 4Q 4R
Nucleoside diphosphate kinase 7 / NDK 7 / NDP kinase 7 / nm23-H7

Details:

Mass: 42545.707 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y5B8, nucleoside-diphosphate kinase

#53: Protein

X Y x y
Nucleoside diphosphate kinase homolog 5 / NDK-H 5 / NDP kinase homolog 5 / Inhibitor of p53-induced apoptosis-beta / IPIA-beta / Testis-specific nm23 homolog / nm23-H5

Details:

Mass: 24265.227 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P56597

Piercer of microtubule wall ... , 2 types, 5 molecules 4S 4T 4U 4V 4W

#25: Protein

4S 4T Piercer of microtubule wall 1 protein / Pierce1 / UPF0691 protein C9orf116 / p53-induced expression in RB-null cells protein 1

Details:

Mass: 15278.101 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5BN46

#26: Protein

4U 4V 4W Piercer of microtubule wall 2 protein

Details:

Mass: 13778.468 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: H3BRN8

RIB43A-like with coiled-coils protein ... , 2 types, 6 molecules 5A 5B 5C 5D 5E 5F

#28: Protein

5A 5B 5C RIB43A-like with coiled-coils protein 2

Details:

Mass: 45510.234 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H4K1

#29: Protein

5D 5E 5F RIB43A-like with coiled-coils protein 1

Details:

Mass: 44074.965 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8N443

Uncharacterized protein ... , 2 types, 4 molecules 5L 5M 5X 5Y

#31: Protein

5L 5M Uncharacterized protein CFAP68 / cilia and flagella associated protein 68

Details:

Mass: 17806.924 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H5F2

#33: Protein

5X 5Y Uncharacterized protein CFAP90 / Cilia and flagella associated protein 90

Details:

Mass: 17019.980 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A4QMS7

Radial spoke head ... , 5 types, 30 molecules A B C D a b c d E F e f G H I J g h i j M N O P m n o p T t

#41: Protein

A B C D a b c d
Radial spoke head 1 homolog / Cancer/testis antigen 79 / CT79 / Male meiotic metaphase chromosome-associated acidic protein / Meichroacidin / Testis-specific gene A2 protein

Details:

Mass: 35165.625 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8WYR4

#44: Protein

E F e f
Radial spoke head protein 3 homolog / A-kinase anchor protein RSPH3 / Radial spoke head-like protein 2

Details:

Mass: 63775.840 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q86UC2

#45: Protein

G H I J g h i j
Radial spoke head protein 4 homolog A / Radial spoke head-like protein 3

Details:

Mass: 80806.805 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5TD94

#47: Protein

M N O P m n o p
Radial spoke head protein 9 homolog

Details:

Mass: 31327.781 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H1X1

#50: Protein

T t Radial spoke head 14 homolog / Rhabdoid tumor deletion region protein 1

Details:

Mass: 38639.023 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UHP6

Dynein axonemal heavy chain ... , 4 types, 8 molecules g2 g4 g3 k8 n0 p0 r0 t0

#71: Protein

g2 g4 Dynein axonemal heavy chain 7 / Axonemal beta dynein heavy chain 7 / Ciliary dynein heavy chain 7 / Dynein heavy chain-like protein 2 / hDHC2

Details:

Mass: 461727.281 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8WXX0

#72: Protein

g3 Dynein axonemal heavy chain 3 / Axonemal beta dynein heavy chain 3 / HsADHC3 / Ciliary dynein heavy chain 3 / Dnahc3-b

Details:

Mass: 471335.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TD57

#91: Protein

k8 Dynein axonemal heavy chain 10

Details:

Mass: 529417.625 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A0A669KB38

#103: Protein

n0 p0 r0 t0
Dynein axonemal heavy chain 5 / Axonemal beta dynein heavy chain 5 / Ciliary dynein heavy chain 5

Details:

Mass: 529657.312 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TE73

Coiled-coil domain-containing protein ... , 4 types, 6 molecules h1 h2 h3 h4 i1 i2

#75: Protein

h1 h2 Coiled-coil domain-containing protein 39

Details:

Mass: 110085.844 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UFE4

#76: Protein

h3 h4 Coiled-coil domain-containing protein 40

Details:

Mass: 130300.141 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q4G0X9

#77: Protein

i1 Coiled-coil domain-containing protein 96

Details:

Mass: 62805.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q2M329

#78: Protein

i2 Coiled-coil domain-containing protein 113

Details:

Mass: 44299.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H0I3

Dynein axonemal intermediate chain ... , 5 types, 11 molecules k1 k2 k3 m1 o1 q1 s1 m2 o2 q2 s2

#86: Protein

k1 Dynein axonemal intermediate chain 3 / Testis development protein NYD-SP29 / WD repeat-containing protein 63

Details:

Mass: 103068.508 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8IWG1

#87: Protein

k2 Dynein axonemal intermediate chain 4 / WD repeat-containing protein 78

Details:

Mass: 94682.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5VTH9

#88: Protein

k3 Dynein axonemal intermediate chain 7 / Cancer susceptibility candidate gene 1 protein / Protein CASC1 / Cilia and flagella associated protein 94 / Lung adenoma susceptibility 1-like protein / Protein phosphatase 1 regulatory subunit 54

Details:

Mass: 83272.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6TDU7

#96: Protein

m1 o1 q1 s1
Dynein axonemal intermediate chain 1 / Axonemal dynein intermediate chain 1

Details:

Mass: 79393.531 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UI46

#97: Protein

m2 o2 q2 s2
Dynein axonemal intermediate chain 2 / Axonemal dynein intermediate chain 2

Details:

Mass: 68897.297 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9GZS0

Dynein light chain roadblock-type ... , 2 types, 8 molecules k4 m4 o4 q4 k5 m5 o5 q5

#89: Protein

k4 m4 o4 q4
Dynein light chain roadblock-type 1 / Bithoraxoid-like protein / BLP / Dynein light chain 2A / cytoplasmic / Dynein-associated protein Km23 / Roadblock domain-containing protein 1

Details:

Mass: 10934.576 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NP97

#90: Protein

k5 m5 o5 q5
Dynein light chain roadblock-type 2 / Dynein light chain 2B / cytoplasmic / Roadblock domain-containing protein 2

Details:

Mass: 10870.625 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8TF09

Dynein light chain Tctex-type ... , 2 types, 8 molecules l7 n7 p7 r7 l8 n8 p8 r8

#93: Protein

l7 n7 p7 r7
Dynein light chain Tctex-type protein 2B / Tctex1 domain-containing protein 2

Details:

Mass: 16139.259 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q8WW35

#94: Protein

l8 n8 p8 r8
Dynein light chain Tctex-type 1 / Protein CW-1 / T-complex testis-specific protein 1 homolog

Details:

Mass: 12461.996 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P63172

Outer dynein arm-docking complex subunit ... , 4 types, 18 molecules m6 o6 q6 s6 u6 m7 q7 s7 u7 m8 o8 q8 s8 u8 m9 q9 s9 u9

#99: Protein

m6 o6 q6 s6 u6
Outer dynein arm-docking complex subunit 1 / Coiled-coil domain-containing protein 114

Details:

Mass: 75158.281 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96M63

#100: Protein

m7 q7 s7 u7
Outer dynein arm-docking complex subunit 2 / Armadillo repeat-containing protein 4

Details:

Mass: 115841.273 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q5T2S8

#101: Protein

m8 o8 q8 s8 u8
Outer dynein arm-docking complex subunit 3 / Coiled-coil domain-containing protein 151

Details:

Mass: 69269.727 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: A5D8V7

#102: Protein

m9 q9 s9 u9
Outer dynein arm-docking complex subunit 4 / Tetratricopeptide repeat protein 25 / TPR repeat protein 25

Details:

Mass: 76771.516 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96NG3

Antibody , 5 types, 8 molecules g1 g5 g6 k9 n9 p9 r9 t9

#70: Antibody

g1 Dynein axonemal heavy chain 12

Details:

Mass: 454871.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: E9PG32

#73: Antibody

g5 Dynein axonemal heavy chain 6 / Axonemal beta dynein heavy chain 6 / Ciliary dynein heavy chain 6

Details:

Mass: 476543.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9C0G6

#74: Antibody

g6 Dynein axonemal heavy chain 1 / Axonemal beta dynein heavy chain 1 / Ciliary dynein heavy chain 1 / Heat shock regulated protein 1 / HSRF-1 / hDHC7

Details:

Mass: 488047.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P2D7

#92: Antibody

k9 Dynein axonemal heavy chain 2 / Axonemal beta dynein heavy chain 2 / Ciliary dynein heavy chain 2 / Dynein heavy chain domain-containing protein 3

Details:

Mass: 508311.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P225

#104: Antibody

n9 p9 r9 t9
Dynein axonemal heavy chain 9 / Axonemal beta dynein heavy chain 9 / Ciliary dynein heavy chain 9

Details:

Mass: 512482.094 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NYC9

Non-polymers , 3 types, 859 molecules

#107: Chemical

AZ-501 AC-501 AE-501 AG-501 AI-501 AK-501 AM-501 AO-501 AQ-501 AS-501 AU-501 AW-501 AY-501 BA-501 BC-501 BE-501 BG-501 BI-501 BK-501 BM-501 ...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE

Details:

Mass: 523.180 Da / Num. of mol.: 285 / Source method: obtained synthetically / Formula: C₁₀H₁₆N₅O₁₄P₃ / Comment: GTP, energy-carrying molecule*YM

#108: Chemical

AZ-502 AC-502 AE-502 AG-502 AI-502 AK-502 AM-502 AO-502 AQ-502 AS-502 AU-502 AW-502 AY-502 BA-502 BC-502 BE-502 BG-502 BI-502 BK-502 BM-502 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 285 / Source method: obtained synthetically / Formula: Mg

#109: Chemical

AB-501 AD-501 AF-501 AH-501 AJ-501 AL-501 AN-501 AP-501 AR-501 AT-501 AV-501 AX-501 BB-501 BD-501 BF-501 BH-501 BJ-501 BL-501 BN-501 BP-501 ...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE

Details:

Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 289 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₁P₂ / Comment: GDP, energy-carrying molecule*YM

Details

• Has ligand of interest: N

Experimental details

Experiment

• Experiment: Method: ELECTRON MICROSCOPY
• EM experiment: Aggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

Sample preparation

• Component: Name: axonemal complex / Type: COMPLEX / Entity ID: #1-#106 / Source: NATURAL
• Molecular weight: Experimental value: NO
• Source (natural): Organism: Homo sapiens (human)
• Buffer solution: pH: 7.3
• Specimen: Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
• Specimen support: Grid material: COPPER / Grid type: Quantifoil R2/1
• Vitrification: Cryogen name: ETHANE

Electron microscopy imaging

• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company
• Microscopy: Model: FEI TITAN KRIOS
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
• Electron lens: Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm
• Specimen holder: Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
• Image recording: Electron dose: 60 e/Ų / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

Processing

EM software

ID	Name	Category
1	RELION	particle selection
2	SerialEM	image acquisition
13	PHENIX	model refinement

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Particle selection: Num. of particles selected: 2582833
• 3D reconstruction: Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 90482 / Symmetry type: POINT