|Entry||Database: EMDB / ID: 3978|
|Title||EM map of HasR, a TonB dependent hemophore receptor from Serratia marcescens.|
|Map data||HasR is a TonB dependent hemophore receptor from Serratia marcescens.|
|Sample||HasR with an amphipol corona covering its membrane embedded part|
|Source||Serratia marcescens (bacteria)|
|Method||single particle reconstruction / 23 Å resolution|
|Authors||Prochnicka-Chalufour A / Wojtowicz H / Pehau Arnaudet G / Gubellini F / Fronzes R / Pruneyre-Izadi N|
|Citation||Journal: Biochem. J. / Year: 2016|
Title: Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach.
Authors: Halina Wojtowicz / Ada Prochnicka-Chalufour / Gisele Cardoso de Amorim / Olga Roudenko / Catherine Simenel / Idir Malki / Gérard Pehau-Arnaudet / Francesca Gubellini / Alexandros Koutsioubas / Javier Pérez / Philippe Delepelaire / Muriel Delepierre / Rémi Fronzes / Nadia Izadi-Pruneyre
Abstract: Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling ...Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70-90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners.
|Date||Deposition: Nov 8, 2017 / Header (metadata) release: Nov 22, 2017 / Map release: Nov 22, 2017 / Last update: Nov 22, 2017|
|Structure viewer||EM map: |
Downloads & links
|File||emd_3978.map.gz (map file in CCP4 format, 16385 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.9 Å|
CCP4 map header:
-Entire HasR with an amphipol corona covering its membrane embedded part
|Entire||Name: HasR with an amphipol corona covering its membrane embedded part|
Number of components: 2
|Mass||Experimental: 950 kDa|
-Component #1: cellular-component, HasR with an amphipol corona covering its mem...
|Cellular-component||Name: HasR with an amphipol corona covering its membrane embedded part|
Recombinant expression: No
|Mass||Experimental: 950 kDa|
|Source||Species: Serratia marcescens (bacteria)|
|Source (engineered)||Expression System: Escherichia coli (E. coli)|
-Component #2: protein, HasR, a TonB dependent hemophore receptor
|Protein||Name: HasR, a TonB dependent hemophore receptor / Recombinant expression: No|
|Specimen||Specimen state: particle|
|Sample solution||pH: 7.5|
|Vitrification||Cryogen name: NONE|
-Electron microscopy imaging
Model: Tecnai F20 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F20|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 20 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: FEI FALCON II (4k x 4k)|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 14158|
|3D reconstruction||Software: RELION / Resolution: 23 Å / Resolution method: FSC 0.143 CUT-OFF|
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