|Entry||Database: EMDB / ID: 3960|
|Title||Cryo-EM Structure of a Licensed DNA Replication Origin|
|Sample||Saccharomyces cerevisiae DNA bound unphosphorylated MCM double hexamer:|
|Function / homology||P-loop containing nucleoside triphosphate hydrolase / Mini-chromosome maintenance protein / MCM domain / MCM OB domain / AAA+ ATPase domain / DNA replication licensing factor Mcm2 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm6 ...P-loop containing nucleoside triphosphate hydrolase / Mini-chromosome maintenance protein / MCM domain / MCM OB domain / AAA+ ATPase domain / DNA replication licensing factor Mcm2 / DNA replication licensing factor Mcm3 / Mini-chromosome maintenance complex protein 4 / DNA replication licensing factor Mcm5 / DNA replication licensing factor Mcm6 / MCM P-loop domain / DNA replication licensing factor Mcm7 / Mini-chromosome maintenance protein 2 / MCM N-terminal domain / MCM OB domain / MCM family signature. / MCM family domain profile. / Nucleic acid-binding, OB-fold / Orc1 removal from chromatin / Mini-chromosome maintenance, conserved site / Activation of the pre-replicative complex / Switching of origins to a post-replicative state / MCM N-terminal domain / MCM complex binding / negative regulation of ATP-dependent DNA helicase activity / MCM core complex / CMG complex / nuclear DNA replication / mitotic DNA replication initiation / negative regulation of chromatin silencing at telomere / MCM complex / establishment of chromatin silencing / regulation of DNA-dependent DNA replication initiation / nuclear pre-replicative complex / DNA replication preinitiation complex / pre-replicative complex assembly involved in nuclear cell cycle DNA replication / single-stranded DNA-dependent ATPase activity / double-strand break repair via break-induced replication / nuclear replication fork / DNA strand elongation involved in DNA replication / replication fork protection complex / DNA unwinding involved in DNA replication / DNA duplex unwinding / chromatin silencing at silent mating-type cassette / DNA helicase activity / DNA replication origin binding / chromatin silencing at telomere / DNA replication initiation / DNA helicase / helicase activity / chromosome, telomeric region / single-stranded DNA binding / nuclear chromosome, telomeric region / cellular response to DNA damage stimulus / chromatin binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytoplasm / DNA replication licensing factor MCM3 / DNA replication licensing factor MCM2 / Minichromosome maintenance protein 5 / DNA replication licensing factor MCM4 / DNA replication licensing factor MCM7 / DNA replication licensing factor MCM6|
Function and homology information
|Source||Saccharomyces cerevisiae (baker's yeast)|
|Method||single particle reconstruction / cryo EM / 6.92 Å resolution|
|Authors||Abid Ali F / Douglas ME / Locke J / Pye VE / Nans A / Diffley JFX / Costa A|
|Citation||Journal: Nat Commun / Year: 2017|
Title: Cryo-EM structure of a licensed DNA replication origin.
Authors: Ferdos Abid Ali / Max E Douglas / Julia Locke / Valerie E Pye / Andrea Nans / John F X Diffley / Alessandro Costa
|Validation Report||PDB-ID: 6f0l|
SummaryFull reportAbout validation report
|Date||Deposition: Nov 2, 2017 / Header (metadata) release: Dec 6, 2017 / Map release: Dec 6, 2017 / Last update: Jan 24, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_3960.map.gz (map file in CCP4 format, 67109 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.38 Å|
CCP4 map header:
-Entire Saccharomyces cerevisiae DNA bound unphosphorylated MCM double hexamer
|Entire||Name: Saccharomyces cerevisiae DNA bound unphosphorylated MCM double hexamer|
Number of components: 1
|Mass||Theoretical: 1.2 MDa|
-Component #1: protein, Saccharomyces cerevisiae DNA bound unphosphorylated MCM ...
|Protein||Name: Saccharomyces cerevisiae DNA bound unphosphorylated MCM double hexamer|
Recombinant expression: No
|Mass||Theoretical: 1.2 MDa|
|Source||Species: Saccharomyces cerevisiae (baker's yeast)|
|Source (engineered)||Expression System: Saccharomyces cerevisiae (baker's yeast)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||pH: 7.6|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 4.33 e/Å2 / Illumination mode: OTHER|
|Lens||Imaging mode: OTHER|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 (4k x 4k)|
|Processing||Method: single particle reconstruction / Applied symmetry: C2 (2 fold cyclic) / Number of projections: 15529|
|3D reconstruction||Software: cryoSPARC / Resolution: 6.92 Å / Resolution method: FSC 0.143 CUT-OFF|
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