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- EMDB-3846: STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH -

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基本情報

登録情報
データベース: EMDB / ID: EMD-3846
タイトルSTRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH
マップデータTranscription pre-initiation complex including Polymerase II and the transcription factors TBP, TFIIA, -B, -E, -F and -H
試料
  • 複合体: Pre-Initiation Complex (PIC)
    • 複合体: Polymerase
      • タンパク質・ペプチド: x 12種
    • 複合体: initiation factors
      • タンパク質・ペプチド: x 8種
    • 複合体: Transcription factors
      • タンパク質・ペプチド: x 9種
    • 複合体: nucleic acid
      • DNA: x 2種
  • リガンド: x 3種
キーワードtranscription initiation / TRANSCRIPTION / macromolecular complex
機能・相同性
機能・相同性情報


regulation of mitotic recombination / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / phosphatidylinositol-5-phosphate binding / RNA polymerase III preinitiation complex assembly / RNA polymerase II promoter clearance / transcription factor TFIIIB complex / positive regulation of mitotic recombination ...regulation of mitotic recombination / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / phosphatidylinositol-5-phosphate binding / RNA polymerase III preinitiation complex assembly / RNA polymerase II promoter clearance / transcription factor TFIIIB complex / positive regulation of mitotic recombination / nucleotide-excision repair factor 3 complex / RNA polymerase I general transcription initiation factor binding / transcription factor TFIIE complex / nucleotide-excision repair, preincision complex assembly / DNA translocase activity / regulation of transcription by RNA polymerase III / transcription factor TFIIK complex / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex / transcription factor TFIIF complex / DNA 5'-3' helicase / phosphatidylinositol-3-phosphate binding / transcription factor TFIIA complex / RNA polymerase I preinitiation complex assembly / transcription factor TFIIH core complex / transcription factor TFIIH holo complex / cyclin-dependent protein serine/threonine kinase activator activity / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / DNA binding, bending / RNA Polymerase I Transcription Initiation / transcription preinitiation complex / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 2 Promoter / poly(A)+ mRNA export from nucleus / RNA Pol II CTD phosphorylation and interaction with CE / DNA 3'-5' helicase / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / termination of RNA polymerase II transcription / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / termination of RNA polymerase III transcription / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / RNA Polymerase II Pre-transcription Events / RNA-templated transcription / 3'-5' DNA helicase activity / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / RNA Polymerase I Promoter Escape / termination of RNA polymerase I transcription / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription initiation at RNA polymerase I promoter / ATPase activator activity / RNA polymerase II complex binding / Estrogen-dependent gene expression / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / nuclear-transcribed mRNA catabolic process / positive regulation of translational initiation / transcription by RNA polymerase III / protein phosphatase activator activity / Dual incision in TC-NER / ATP-dependent activity, acting on DNA / positive regulation of transcription initiation by RNA polymerase II / translesion synthesis / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / RNA polymerase II preinitiation complex assembly / transcription by RNA polymerase I / translation initiation factor binding / transcription-coupled nucleotide-excision repair / DNA helicase activity / TBP-class protein binding / nucleotide-excision repair / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / DNA-templated transcription initiation / P-body / positive regulation of transcription elongation by RNA polymerase II / ribonucleoside binding / mRNA processing / DNA-directed RNA polymerase / cytoplasmic stress granule / disordered domain specific binding / DNA-directed RNA polymerase activity / ubiquitin protein ligase activity / peroxisome / ribosome biogenesis
類似検索 - 分子機能
: / Tfa2 E-tether / Bacterial type XPD DNA helicase, FeS cluster domain / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain ...: / Tfa2 E-tether / Bacterial type XPD DNA helicase, FeS cluster domain / TFIIH subunit Tfb4/GTF2H3 / Transcription factor Tfb4 / TFIIH C1-like domain / Ssl1-like / TFIIH subunit Ssl1/p44 / Ssl1-like / TFIIH C1-like domain / TFIIH C1-like domain / TFIIH p62 subunit, N-terminal / TFIIH subunit Tfb1/GTF2H1 / TFIIH p62 subunit, N-terminal domain / BSD domain / BSD domain superfamily / BSD domain / BSD domain profile. / domain in transcription factors and synapse-associated proteins / RAD3/XPD family / Helicase XPB/Ssl2 / ERCC3/RAD25/XPB helicase, C-terminal domain / Helicase XPB/Ssl2, N-terminal domain / Helicase conserved C-terminal domain / ERCC3/RAD25/XPB C-terminal helicase / Helical and beta-bridge domain / Helical and beta-bridge domain / Transcription factor TFIIH subunit p52/Tfb2 / Transcription factor Tfb2, C-terminal domain / Transcription factor Tfb2 / Transcription factor Tfb2 (p52) C-terminal domain / Transcription factor TFIIE beta subunit, DNA-binding domain / TFIIH subunit TTDA/Tfb5 / Transcription initiation factor TFIIE, beta subunit / TFB5-like superfamily / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / Transcription factor TFIIH complex subunit Tfb5 / TFA2 Winged helix domain 2 / TFIIE beta central core DNA-binding domain profile. / Transcription factor TFIIH complex subunit Tfb5 / ATP-dependent helicase Rad3/Chl1-like / : / Cdk-activating kinase assembly factor MAT1/Tfb3 / Cdk-activating kinase assembly factor MAT1, centre / CDK-activating kinase assembly factor MAT1 / Zinc finger, C3HC4 type (RING finger) / Helicase-like, DEXD box c2 type / DEAD2 / DEAD_2 / DEXDc2 / Helicase superfamily 1/2, DinG/Rad3-like / HELICc2 / ATP-dependent helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-type / Helicase C-terminal domain / Superfamilies 1 and 2 helicase ATP-binding type-2 domain profile. / Transcription initiation factor IIE subunit alpha, N-terminal / Transcription factor TFE/TFIIEalpha HTH domain / TFIIEalpha/SarR/Rpc3 HTH domain / Transcription factor E / TFIIE alpha subunit / TFE/IIEalpha-type HTH domain profile. / Transcription initiation factor IIE / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIA, gamma subunit / Transcription factor IIA, alpha-helical domain / Transcription factor IIA, beta-barrel / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / Transcription initiation factor IIA, gamma subunit, helical domain / Transcription initiation factor IIA, gamma subunit / Transcription initiation factor IIF, beta subunit / TFIIF beta subunit, HTH domain / TFIIF, beta subunit, N-terminal / TFIIF, beta subunit HTH domain / TFIIF, beta subunit N-terminus / Transcription initiation factor IIF, alpha subunit / Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) / Transcription Factor IIF, Rap30/Rap74, interaction / Transcription factor TFIIB, cyclin-like domain / Transcription factor TFIIB, conserved site / Transcription factor TFIIB repeat / Transcription factor TFIIB repeat signature. / Zinc finger TFIIB-type profile. / Transcription factor TFIIB / Zinc finger, TFIIB-type / Helicase/UvrB, N-terminal / Type III restriction enzyme, res subunit / TFIIB zinc-binding / TATA-box binding protein, eukaryotic / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / TBP domain superfamily / DEAH-box subfamily ATP-dependent helicases signature.
類似検索 - ドメイン・相同性
DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 ...DNA-directed RNA polymerase II subunit RPB1 / General transcription and DNA repair factor IIH helicase subunit XPD/RAD3 / DNA-directed RNA polymerase II subunit RPB2 / TATA-box-binding protein / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Transcription initiation factor IIA large subunit / Transcription initiation factor IIA subunit 2 / General transcription and DNA repair factor IIH subunit TFB1 / DNA-directed RNA polymerase II subunit RPB7 / Transcription initiation factor IIE subunit alpha / Transcription initiation factor IIE subunit beta / DNA-directed RNA polymerase II subunit RPB11 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 / General transcription and DNA repair factor IIH subunit TFB2 / RNA polymerase II transcription factor B subunit 3 / General transcription and DNA repair factor IIH subunit SSL1 / General transcription and DNA repair factor IIH subunit TFB4 / General transcription and DNA repair factor IIH subunit TFB5
類似検索 - 構成要素
生物種Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) / synthetic construct (人工物)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.7 Å
データ登録者Schilbach S / Hantsche M / Tegunov D / Dienemann C / Wigge C / Urlaub H / Cramer P
資金援助 ドイツ, 4件
OrganizationGrant number国
German Research FoundationSFB860 ドイツ
German Research FoundationSPP1935 ドイツ
European Research Council693023 ドイツ
Volkswagen Foundation ドイツ
引用ジャーナル: Nature / 年: 2017
タイトル: Structures of transcription pre-initiation complex with TFIIH and Mediator.
著者: S Schilbach / M Hantsche / D Tegunov / C Dienemann / C Wigge / H Urlaub / P Cramer /
要旨: For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron ...For the initiation of transcription, RNA polymerase II (Pol II) assembles with general transcription factors on promoter DNA to form the pre-initiation complex (PIC). Here we report cryo-electron microscopy structures of the Saccharomyces cerevisiae PIC and PIC-core Mediator complex at nominal resolutions of 4.7 Å and 5.8 Å, respectively. The structures reveal transcription factor IIH (TFIIH), and suggest how the core and kinase TFIIH modules function in the opening of promoter DNA and the phosphorylation of Pol II, respectively. The TFIIH core subunit Ssl2 (a homologue of human XPB) is positioned on downstream DNA by the 'E-bridge' helix in TFIIE, consistent with TFIIE-stimulated DNA opening. The TFIIH kinase module subunit Tfb3 (MAT1 in human) anchors the kinase Kin28 (CDK7), which is mobile in the PIC but preferentially located between the Mediator hook and shoulder in the PIC-core Mediator complex. Open spaces between the Mediator head and middle modules may allow access of the kinase to its substrate, the C-terminal domain of Pol II.
履歴
登録2017年8月11日-
ヘッダ(付随情報) 公開2018年4月25日-
マップ公開2018年4月25日-
更新2024年5月15日-
現状2024年5月15日処理サイト: PDBe / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.066
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.066
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-5oqj
  • 表面レベル: 0.066
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_3846.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_3846.map.gz / 形式: CCP4 / 大きさ: 163.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Transcription pre-initiation complex including Polymerase II and the transcription factors TBP, TFIIA, -B, -E, -F and -H
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 350 pix.
= 479.5 Å		1.37 Å/pix.
x 350 pix.
= 479.5 Å		1.37 Å/pix.
x 350 pix.
= 479.5 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 1.37 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 0.066 / ムービー #1: 0.066
最小 - 最大-0.20505115 - 0.5921751
平均 (標準偏差)0.0014869234 (±0.0113299545)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ350350350
Spacing350350350
セルA=B=C: 479.5 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.371.371.37
M x/y/z350350350
origin x/y/z0.0000.0000.000
length x/y/z479.500479.500479.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS350350350
D min/max/mean-0.2050.5920.001

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添付データ

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試料の構成要素

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全体 : Pre-Initiation Complex (PIC)

全体名称: Pre-Initiation Complex (PIC)
要素
  • 複合体: Pre-Initiation Complex (PIC)
    • 複合体: Polymerase
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB1
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB2
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB3
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB4
      • タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC1
      • タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC2
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB7
      • タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC3
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB9
      • タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC5
      • タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB11
      • タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC4
    • 複合体: initiation factors
      • タンパク質・ペプチド: Transcription initiation factor IIB
      • タンパク質・ペプチド: TATA-box-binding protein
      • タンパク質・ペプチド: Transcription initiation factor IIF subunit alpha
      • タンパク質・ペプチド: Transcription initiation factor IIF subunit beta
      • タンパク質・ペプチド: Transcription initiation factor IIA large subunit
      • タンパク質・ペプチド: Transcription initiation factor IIA subunit 2
      • タンパク質・ペプチド: Transcription initiation factor IIE subunit alpha
      • タンパク質・ペプチド: Transcription initiation factor IIE subunit beta
    • 複合体: Transcription factors
      • タンパク質・ペプチド: DNA repair helicase RAD3
      • タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 1
      • タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 2
      • タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 3
      • タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 4
      • タンパク質・ペプチド: RNA polymerase II transcription factor B subunit 5
      • タンパク質・ペプチド: Suppressor of stem-loop protein 1
      • タンパク質・ペプチド: DNA repair helicase RAD25
      • タンパク質・ペプチド: Unknown protein
    • 複合体: nucleic acid
      • DNA: NONTEMPLATE DNA
      • DNA: TEMPLATE DNA
  • リガンド: ZINC ION
  • リガンド: MAGNESIUM ION
  • リガンド: IRON/SULFUR CLUSTER

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超分子 #1: Pre-Initiation Complex (PIC)

超分子名称: Pre-Initiation Complex (PIC) / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#31 / 詳細: PRE-INITIATION COMPLEX WITH TFIIH
分子量理論値: 1.43 MDa

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超分子 #2: Polymerase

超分子名称: Polymerase / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1-#12 / 詳細: natural source entities
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)

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超分子 #3: initiation factors

超分子名称: initiation factors / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #13, #15-#17, #19-#22 / 詳細: entitites expressed in coli
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)

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超分子 #4: Transcription factors

超分子名称: Transcription factors / タイプ: complex / ID: 4 / 親要素: 1 / 含まれる分子: #23-#31 / 詳細: entitites expressed in T.ni
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)

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超分子 #5: nucleic acid

超分子名称: nucleic acid / タイプ: complex / ID: 5 / 親要素: 1 / 含まれる分子: #14, #18 / 詳細: Synthetic nucleic acid
由来(天然)生物種: synthetic construct (人工物)

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分子 #1: DNA-directed RNA polymerase II subunit RPB1

分子名称: DNA-directed RNA polymerase II subunit RPB1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA-directed RNA polymerase
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 191.821578 KDa
配列文字列: MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKP VFHVGFIAKI KKVCECVCMH CGKLLLDEHN ELMRQALAIK DSKKRFAAIW TLCKTKMVCE TDVPSEDDPT Q LVSRGGCG ...文字列:
MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKP VFHVGFIAKI KKVCECVCMH CGKLLLDEHN ELMRQALAIK DSKKRFAAIW TLCKTKMVCE TDVPSEDDPT Q LVSRGGCG NTQPTIRKDG LKLVGSWKKD RATGDADEPE LRVLSTEEIL NIFKHISVKD FTSLGFNEVF SRPEWMILTC LP VPPPPVR PSISFNESQR GEDDLTFKLA DILKANISLE TLEHNGAPHH AIEEAESLLQ FHVATYMDND IAGQPQALQK SGR PVKSIR ARLKGKEGRI RGNLMGKRVD FSARTVISGD PNLELDQVGV PKSIAKTLTY PEVVTPYNID RLTQLVRNGP NEHP GAKYV IRDSGDRIDL RYSKRAGDIQ LQYGWKVERH IMDNDPVLFN RQPSLHKMSM MAHRVKVIPY STFRLNLSVT SPYNA DFDG DEMNLHVPQS EETRAELSQL CAVPLQIVSP QSNKPCMGIV QDTLCGIRKL TLRDTFIELD QVLNMLYWVP DWDGVI PTP AIIKPKPLWS GKQILSVAIP NGIHLQRFDE GTTLLSPKDN GMLIIDGQII FGVVEKKTVG SSNGGLIHVV TREKGPQ VC AKLFGNIQKV VNFWLLHNGF STGIGDTIAD GPTMREITET IAEAKKKVLD VTKEAQANLL TAKHGMTLRE SFEDNVVR F LNEARDKAGR LAEVNLKDLN NVKQMVMAGS KGSFINIAQM SACVGQQSVE GKRIAFGFVD RTLPHFSKDD YSPESKGFV ENSYLRGLTP QEFFFHAMGG REGLIDTAVK TAETGYIQRR LVKALEDIMV HYDNTTRNSL GNVIQFIYGE DGMDAAHIEK QSLDTIGGS DAAFEKRYRV DLLNTDHTLD PSLLESGSEI LGDLKLQVLL DEEYKQLVKD RKFLREVFVD GEANWPLPVN I RRIIQNAQ QTFHIDHTKP SDLTIKDIVL GVKDLQENLL VLRGKNEIIQ NAQRDAVTLF CCLLRSRLAT RRVLQEYRLT KQ AFDWVLS NIEAQFLRSV VHPGEMVGVL AAQSIGEPAT QMTLNTFHFA GVASKKVTSG VPRLKEILNV AKNMKTPSLT VYL EPGHAA DQEQAKLIRS AIEHTTLKSV TIASEIYYDP DPRSTVIPED EEIIQLHFSL LDEEAEQSFD QQSPWLLRLE LDRA AMNDK DLTMGQVGER IKQTFKNDLF VIWSEDNDEK LIIRCRVVRP KSLDAETEAE EDHMLKKIEN TMLENITLRG VENIE RVVM MKYDRKVPSP TGEYVKEPEW VLETDGVNLS EVMTVPGIDP TRIYTNSFID IMEVLGIEAG RAALYKEVYN VIASDG SYV NYRHMALLVD VMTTQGGLTS VTRHGFNRSN TGALMRCSFE ETVEILFEAG ASAELDDCRG VSENVILGQM APIGTGA FD VMIDEESLVK YMPEQKITEI EDGQDGGVTP YSNESGLVNA DLDVKDELMF SPLVDSGSND AMAGGFTAYG GADYGEAT S PFGAYGEAPT SPGFGVSSPG FSPTSPTYSP TSPAYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPA YSPTSPSYSP TSPSYSPTSP SYSPTSPSY SPTSPNYSPT SPSYSPTSPG YSPGSPAYSP KQDEQKHNEN ENSR

UniProtKB: DNA-directed RNA polymerase II subunit RPB1

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分子 #2: DNA-directed RNA polymerase II subunit RPB2

分子名称: DNA-directed RNA polymerase II subunit RPB2 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA-directed RNA polymerase
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 138.937297 KDa
配列文字列: MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYE ISFGKIYVTK PMVNESDGVT HALYPQEARL RNLTYSSGLF VDVKKRTYEA IDVPGRELKY ELIAEESEDD S ESGKVFIG ...文字列:
MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYE ISFGKIYVTK PMVNESDGVT HALYPQEARL RNLTYSSGLF VDVKKRTYEA IDVPGRELKY ELIAEESEDD S ESGKVFIG RLPIMLRSKN CYLSEATESD LYKLKECPFD MGGYFIINGS EKVLIAQERS AGNIVQVFKK AAPSPISHVA EI RSALEKG SRFISTLQVK LYGREGSSAR TIKATLPYIK QDIPIVIIFR ALGIIPDGEI LEHICYDVND WQMLEMLKPC VED GFVIQD RETALDFIGR RGTALGIKKE KRIQYAKDIL QKEFLPHITQ LEGFESRKAF FLGYMINRLL LCALDRKDQD DRDH FGKKR LDLAGPLLAQ LFKTLFKKLT KDIFRYMQRT VEEAHDFNMK LAINAKTITS GLKYALATGN WGEQKKAMSS RAGVS QVLN RYTYSSTLSH LRRTNTPIGR DGKLAKPRQL HNTHWGLVCP AETPEGQACG LVKNLSLMSC ISVGTDPMPI ITFLSE WGM EPLEDYVPHQ SPDATRVFVN GVWHGVHRNP ARLMETLRTL RRKGDINPEV SMIRDIREKE LKIFTDAGRV YRPLFIV ED DESLGHKELK VRKGHIAKLM ATEYQDIEGG FEDVEEYTWS SLLNEGLVEY IDAEEEESIL IAMQPEDLEP AEANEEND L DVDPAKRIRV SHHATTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS AMGKQAMGVF LTNYNVRMDT MANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGT YDKLDDDGLI APGVRVSGED VIIGKTTPIS PDEEELGQRT AYHSKRDAST PLRSTENGIV DQVLVTTNQD G LKFVKVRV RTTKIPQIGD KFASRHGQKG TIGITYRRED MPFTAEGIVP DLIINPHAIP SRMTVAHLIE CLLSKVAALS GN EGDASPF TDITVEGISK LLREHGYQSR GFEVMYNGHT GKKLMAQIFF GPTYYQRLRH MVDDKIHARA RGPMQVLTRQ PVE GRSRDG GLRFGEMERD CMIAHGAASF LKERLMEASD AFRVHICGIC GLMTVIAKLN HNQFECKGCD NKIDIYQIHI PYAA KLLFQ ELMAMNITPR LYTDRSRDF

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

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分子 #3: DNA-directed RNA polymerase II subunit RPB3

分子名称: DNA-directed RNA polymerase II subunit RPB3 / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 35.330457 KDa
配列文字列: MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCE DHCDKCSVVL TLQAFGESES TTNVYSKDLV IVSNLMGRNI GHPIIQDKEG NGVLICKLRK GQELKLTCVA K KGIAKEHA ...文字列:
MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCE DHCDKCSVVL TLQAFGESES TTNVYSKDLV IVSNLMGRNI GHPIIQDKEG NGVLICKLRK GQELKLTCVA K KGIAKEHA KWGPAAAIEF EYDPWNKLKH TDYWYEQDSA KEWPQSKNCE YEDPPNEGDP FDYKAQADTF YMNVESVGSI PV DQVVVRG IDTLQKKVAS ILLALTQMDQ DKVNFASGDN NTASNMLGSN EDVMMTGAEQ DPYSNASQMG NTGSGGYDNA W

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

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分子 #4: DNA-directed RNA polymerase II subunit RPB4

分子名称: DNA-directed RNA polymerase II subunit RPB4 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 25.451191 KDa
配列文字列: MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENAND ETTAVEDEDD DLDEDDVNAD DDDFMHSETR EKELESIDVL LEQTTGGNNK DLKNTMQYLT NFSRFRDQET V GAVIQLLK ...文字列:
MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENAND ETTAVEDEDD DLDEDDVNAD DDDFMHSETR EKELESIDVL LEQTTGGNNK DLKNTMQYLT NFSRFRDQET V GAVIQLLK STGLHPFEVA QLGSLACDTA DEAKTLIPSL NNKISDDELE RILKELSNLE TLY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

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分子 #5: DNA-directed RNA polymerases I, II, and III subunit RPABC1

分子名称: DNA-directed RNA polymerases I, II, and III subunit RPABC1
タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 25.117094 KDa
配列文字列: MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVG VKTMKTFVIH IQEKNFQTGI FVYQNNITPS AMKLVPSIPP ATIETFNEAA LVVNITHHEL VPKHIRLSSD E KRELLKRY ...文字列:
MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVG VKTMKTFVIH IQEKNFQTGI FVYQNNITPS AMKLVPSIPP ATIETFNEAA LVVNITHHEL VPKHIRLSSD E KRELLKRY RLKESQLPRI QRADPVALYL GLKRGEVVKI IRKSETSGRY ASYRICM

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

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分子 #6: DNA-directed RNA polymerases I, II, and III subunit RPABC2

分子名称: DNA-directed RNA polymerases I, II, and III subunit RPABC2
タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 17.931834 KDa
配列文字列:
MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYE RARILGTRAL QISMNAPVFV DLEGETDPLR IAMKELAEKK IPLVIRRYLP DGSFEDWSVE ELIVDL

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

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分子 #7: DNA-directed RNA polymerase II subunit RPB7

分子名称: DNA-directed RNA polymerase II subunit RPB7 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 19.081053 KDa
配列文字列:
MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDG TVVSCSQHGF EVQVGPMKVF VTKHLMPQDL TFNAGSNPPS YQSSEDVITI KSRIRVKIEG CISQVSSIHA I GSIKEDYL GAI

UniProtKB: DNA-directed RNA polymerase II subunit RPB7

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分子 #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

分子名称: DNA-directed RNA polymerases I, II, and III subunit RPABC3
タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 16.525363 KDa
配列文字列:
MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSL ADDYDYVMYG TAYKFEEVSK DLIAVYYSFG GLLMRLEGNY RNLNNLKQEN AYLLIRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

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分子 #9: DNA-directed RNA polymerase II subunit RPB9

分子名称: DNA-directed RNA polymerase II subunit RPB9 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 14.308161 KDa
配列文字列:
MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQ QRRKDTSMVL FFVCLSCSHI FTSDQKNKRT QFS

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

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分子 #10: DNA-directed RNA polymerases I, II, and III subunit RPABC5

分子名称: DNA-directed RNA polymerases I, II, and III subunit RPABC5
タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 8.290732 KDa
配列文字列:
MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF LRYNPLEKRD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

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分子 #11: DNA-directed RNA polymerase II subunit RPB11

分子名称: DNA-directed RNA polymerase II subunit RPB11 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 13.633493 KDa
配列文字列:
MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA YKVEHPFFAR FKLRIQTTEG YDPKDALKN ACNSIINKLG ALKTNFETEW NLQTLAADDA F

UniProtKB: DNA-directed RNA polymerase II subunit RPB11

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分子 #12: DNA-directed RNA polymerases I, II, and III subunit RPABC4

分子名称: DNA-directed RNA polymerases I, II, and III subunit RPABC4
タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 7.729969 KDa
配列文字列:
MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD CGHRILLKAR TKRLVQFEAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

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分子 #13: Transcription initiation factor IIB

分子名称: Transcription initiation factor IIB / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 38.25734 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDG NNLSTRIGKG ETTDMRFTKE LNKAQGKNVM DKKDNEVQAA FAKITMLCDA AELPKIVKDC AKEAYKLCHD E KTLKGKSM ...文字列:
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDG NNLSTRIGKG ETTDMRFTKE LNKAQGKNVM DKKDNEVQAA FAKITMLCDA AELPKIVKDC AKEAYKLCHD E KTLKGKSM ESIMAASILI GCRRAEVART FKEIQSLIHV KTKEFGKTLN IMKNILRGKS EDGFLKIDTD NMSGAQNLTY IP RFCSHLG LPMQVTTSAE YTAKKCKEIK EIAGKSPITI AVVSIYLNIL LFQIPITAAK VGQTLQVTEG TIKSGYKILY EHR DKLVDP QLIANGVVSL DNLPGVEKK

UniProtKB: Transcription initiation factor IIB

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分子 #15: TATA-box-binding protein

分子名称: TATA-box-binding protein / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 27.042275 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHA RNAEYNPKRF AAVIMRIREP KTTALIFASG KMVVTGAKSE DDSKLASRKY ARIIQKIGFA AKFTDFKIQN I VGSCDVKF ...文字列:
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHA RNAEYNPKRF AAVIMRIREP KTTALIFASG KMVVTGAKSE DDSKLASRKY ARIIQKIGFA AKFTDFKIQN I VGSCDVKF PIRLEGLAFS HGTFSSYEPE LFPGLIYRMV KPKIVLLIFV SGKIVLTGAK QREEIYQAFE AIYPVLSEFR KM

UniProtKB: TATA-box-binding protein

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分子 #16: Transcription initiation factor IIF subunit alpha

分子名称: Transcription initiation factor IIF subunit alpha / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 82.32057 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL KFQSKKKINP VTDFHLPVRL HRKDTRNLQF QLTRAEIVQR Q KEISEYKK ...文字列:
MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL KFQSKKKINP VTDFHLPVRL HRKDTRNLQF QLTRAEIVQR Q KEISEYKK KAEQERSTPN SGGMNKSGTV SLNNTVKDGS QTPTVDSVTK DNTANGVNSS IPTVTGSSVP PASPTTVSAV ES NGLSNGS TSAANGLDGN ASTANLANGR PLVTKLEDAG PAEDPTKVGM VKYDGKEVTN EPEFEEGTMD PLADVAPDGG GRA KRGNLR RKTRQLKVLD ENAKKLRFEE FYPWVMEDFD GYNTWVGSYE AGNSDSYVLL SVEDDGSFTM IPADKVYKFT ARNK YATLT IDEAEKRMDK KSGEVPRWLM KHLDNIGTTT TRYDRTRRKL KAVADQQAMD EDDRDDNSEV ELDYDEEFAD DEEAP IIDG NEQENKESEQ RIKKEMLQAN AMGLRDEEAP SENEEDELFG EKKIDEDGER IKKALQKTEL AALYSSDENE INPYLS ESD IENKENESPV KKEEDSDTLS KSKRSSPKKQ QKKATNAHVH KEPTLRVKSI KNCVIILKGD KKILKSFPEG EWNPQTT KA VDSSNNASNT VPSPIKQEEG LNSTVAEREE TPAPTITEKD IIEAIGDGKV NIKEFGKFIR RKYPGAENKK LMFAIVKK L CRKVGNDHME LKKE

UniProtKB: Transcription initiation factor IIF subunit alpha

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分子 #17: Transcription initiation factor IIF subunit beta

分子名称: Transcription initiation factor IIF subunit beta / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 46.684492 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE ...文字列:
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE EELKKKQQQQ KRRNNRKKFN HRVMTDRDGR DRYIPYVKTI PKKTAIVGTV CHECQVMPSM NDPNYHKIVE QR RNIVKLN NKERITTLDE TVGVTMSHTG MSMRSDNSNF LKVGREKAKS NIKSIRMPKK EILDYLFKLF DEYDYWSLKG LKE RTRQPE AHLKECLDKV ATLVKKGPYA FKYTLRPEYK KLKEEERKAT LGELADEQTG SAGDNAQGDA EADLEDEIEM EDVV

UniProtKB: Transcription initiation factor IIF subunit beta

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分子 #19: Transcription initiation factor IIA large subunit

分子名称: Transcription initiation factor IIA large subunit / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 32.230805 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIK EENTGNEGLI LPNINSNNNI PHSGETNINT NTVEATNNSG ATLNTNTSGN TNADVTSQPK IEVKPEIELT I NNANITTV ...文字列:
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIK EENTGNEGLI LPNINSNNNI PHSGETNINT NTVEATNNSG ATLNTNTSGN TNADVTSQPK IEVKPEIELT I NNANITTV ENIDDESEKK DDEEKEEDVE KTRKEKEQIE QVKLQAKKEK RSALLDTDEV GSELDDSDDD YLISEGEEDG PD ENLMLCL YDKVTRTKAR WKCSLKDGVV TINRNDYTFQ KAQVEAEWV

UniProtKB: Transcription initiation factor IIA large subunit

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分子 #20: Transcription initiation factor IIA subunit 2

分子名称: Transcription initiation factor IIA subunit 2 / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 13.47307 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列:
MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVED SHRDASQNGS GDSQSVISVD KLRIVACNSK KSE

UniProtKB: Transcription initiation factor IIA subunit 2

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分子 #21: Transcription initiation factor IIE subunit alpha

分子名称: Transcription initiation factor IIE subunit alpha / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 57.538461 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYV KYPHAIDAIK WKVHQVVQRL KDDLDKNSEP NGYMCPICLT KYTQLEAVQL LNFDRTEFLC SLCDEPLVED D SGKKNKEK ...文字列:
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYV KYPHAIDAIK WKVHQVVQRL KDDLDKNSEP NGYMCPICLT KYTQLEAVQL LNFDRTEFLC SLCDEPLVED D SGKKNKEK QDKLNRLMDQ IQPIIDSLKK IDDSRI(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)EDEFE DVTDTAGTAK TESNTSNDVK QESINDKTED AVNATATASG PSANA KPND GDDDDDDDDD EMDIEFEDV

UniProtKB: Transcription initiation factor IIE subunit alpha, Transcription initiation factor IIE subunit alpha

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分子 #22: Transcription initiation factor IIE subunit beta

分子名称: Transcription initiation factor IIE subunit beta / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 37.050434 KDa
組換発現生物種: Escherichia coli BL21(DE3) (大腸菌)
配列文字列: MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDD DDEDFGSSPS KKVRPGSIAA AALQANQTDI SKSHDSSKLL WATEYIQKKG KPVLVNELLD YLSMKKDDKV I ELLKKLDR ...文字列:
MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDD DDEDFGSSPS KKVRPGSIAA AALQANQTDI SKSHDSSKLL WATEYIQKKG KPVLVNELLD YLSMKKDDKV I ELLKKLDR IEFDPKKGTF KYLSTYDVHS PSELLKLLRS QVTFKGISCK DLKDGWPQCD ETINQLEEDS KILVLRTKKD KT PRYVWYN SGGNLKCIDE EFVKMWENVQ LPQFAELPRK LQDLGLKPAS VDPATIKRQT KRVEVKKKRQ RKGKITNTHM TGI LKDYSH RV

UniProtKB: Transcription initiation factor IIE subunit beta

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分子 #23: DNA repair helicase RAD3

分子名称: DNA repair helicase RAD3 / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 89.064555 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENL MDYRTKELGY QEDFRGLGLT SRKNLCLHPE VSKERKGTVV DEKCRRMTNG QAKRKLEEDP EANVELCEYH E NLYNIEVE ...文字列:
MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENL MDYRTKELGY QEDFRGLGLT SRKNLCLHPE VSKERKGTVV DEKCRRMTNG QAKRKLEEDP EANVELCEYH E NLYNIEVE DYLPKGVFSF EKLLKYCEEK TLCPYFIVRR MISLCNIIIY SYHYLLDPKI AERVSNEVSK DSIVIFDEAH NI DNVCIES LSLDLTTDAL RRATRGANAL DERISEVRKV DSQKLQDEYE KLVQGL(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)RAEHFVSFL KRLIEYLKTR M KVLHVISE TPKSFLQHLK QLTFIERKPL RFCSERLSLL VRTLEVTEVE DFTALKDIAT FATLISTYEE GFLLIIEPYE IE NAAVPNP IMRFTCLDAS IAIKPVFERF SSVIITSGTI SPLDMYPRML NFKTVLQKSY AMTLAKKSFL PMIITKGSDQ VAI SSRFEI RNDPSIVRNY GSMLVEFAKI TPDGMVVFFP SYLYMESIVS MWQTMGILDE VWKHKLILVE TPDAQETSLA LETY RKACS NGRGAILLSV ARGKVSEGID FDHQYGRTVL MIGIPFQYTE SRILKARLEF MRENYRIREN DFLSFDAMRH AAQCL GRVL RGKDDYGVMV LADRRFSRKR SQLPKWIAQG LSDADLNLST DMAISNTKQF LRTMAQPTDP KDQEGVSVWS YEDLIK HQN SRKDQGGFIE NENKEGEQDE DEDEDIEMQ

UniProtKB: General transcription and DNA repair factor IIH helicase subunit XPD/RAD3, General transcription and DNA repair factor IIH helicase subunit XPD/RAD3

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分子 #24: RNA polymerase II transcription factor B subunit 1

分子名称: RNA polymerase II transcription factor B subunit 1 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 65.440922 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMF SFNNRTVMDN IKMTLQQIIS RYKDADIYEE KRRREESAQH TETPMSSSSV TAGTPTPHLD TPQLNNGAPL I NTAKLDDS ...文字列:
MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMF SFNNRTVMDN IKMTLQQIIS RYKDADIYEE KRRREESAQH TETPMSSSSV TAGTPTPHLD TPQLNNGAPL I NTAKLDDS LSKEKLLTNL KLQQSLLKGN KVLMKVFQET VINAGLPPSE FWSTRIPLLR (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)NLSREKI LNIFENYPIV KKAYTDNV P KNFKEPEFWA RFFSSKLFRK (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)EVKST LPIDLLESCR MLHTTCCEFL KHFYIHFQSG EQKQASTVKK LYNHLKDCIE KLNELFQDV LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN

UniProtKB: General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1, General transcription and DNA repair factor IIH subunit TFB1

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分子 #25: RNA polymerase II transcription factor B subunit 2

分子名称: RNA polymerase II transcription factor B subunit 2 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 48.406277 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIP NKSSGTLMIN LNPTFKISLR NAL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK) ...文字列:
MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIP NKSSGTLMIN LNPTFKISLR NAL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)SEKVLNL LKHSKLMEEV NSTGEFKITN EGFQFLLQ(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) KMDLVDVLHF IFMLGALEVG KAYKIDALSE TQRIMLQDMR DYGLVFQKHS NDSIFYPTKL ALMLTSD(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)LPPTVV DQIRLWQLEL DRVITYEGSL YSDFETSQEY NLLSKYAQDI GVLLWKDDKK KKFFISKEG NSQVLDFAKR KLKKKQ

UniProtKB: General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, ...UniProtKB: General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2, General transcription and DNA repair factor IIH subunit TFB2

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分子 #26: RNA polymerase II transcription factor B subunit 3

分子名称: RNA polymerase II transcription factor B subunit 3 / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 38.188438 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVF NVFNKTIDDF NGDLVEYNKY LEEVEDIIYK LDHGIDVAKT EEKLRTYEEL NKQLIMNNLE RSRTEIESFE Q RQKFEKEM ...文字列:
MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVF NVFNKTIDDF NGDLVEYNKY LEEVEDIIYK LDHGIDVAKT EEKLRTYEEL NKQLIMNNLE RSRTEIESFE Q RQKFEKEM KLKKRLLERQ IEEEERMNKE WTKKEIVNRL STTTQDINET IEGVKNTVKL KKSSARRKLE ELNRVLKNNP YF NSNVNVQ NSRLKDAVPF TPFNGDREAH PRFTLKGSVY NDPFIKDLEH RKEFIASGFN TNYAYERVLT EAFMGLGCVI SEE L

UniProtKB: RNA polymerase II transcription factor B subunit 3

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分子 #27: RNA polymerase II transcription factor B subunit 4

分子名称: RNA polymerase II transcription factor B subunit 4 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 36.403598 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYP(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK) ...文字列:
MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYP(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)YRRF RNVDETLVEE IYKLFE LEK KQIEQNSQRS TLAGAMSAGL TYVNRISKES VTTSLKSRLL VLTCGSGSSK DEIFQYIPIM NCIFSATKMK CPIDVVK IG GSKESTFLQQ TTDATNGVYL HVESTEGLIQ YLATAMFIDP (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)TS CYLTGRVVAV GFICSVCLCV LSIIPPGN K CPACDSQFDE HVIAKLKRKP VVPRLKAKKK VTKP

UniProtKB: General transcription and DNA repair factor IIH subunit TFB4, General transcription and DNA repair factor IIH subunit TFB4, General transcription and DNA repair factor IIH subunit TFB4

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分子 #28: RNA polymerase II transcription factor B subunit 5

分子名称: RNA polymerase II transcription factor B subunit 5 / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 8.24349 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列:
MARARKGALV QCDPSIKALI LQIDAKMSDI VLEELDDTHL LVNPSKVEFV KHELNRLLSK NIYNPMDEEE NQ

UniProtKB: General transcription and DNA repair factor IIH subunit TFB5

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分子 #29: Suppressor of stem-loop protein 1

分子名称: Suppressor of stem-loop protein 1 / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 51.122695 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKV DDEGDMASLV ASIVEARKKR TAKKNITPYQ RGIIRSLILT LDCSEAMLEK DLRPNRHAMI IQYAIDFVHE F FDQNPISQ ...文字列:
MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKV DDEGDMASLV ASIVEARKKR TAKKNITPYQ RGIIRSLILT LDCSEAMLEK DLRPNRHAMI IQYAIDFVHE F FDQNPISQ MGIIIMRNGL AQLVSQVSGN PQDHIDALKS IRKQEPKGNP SLQNALEMAR GLLLPVPAHC TREVLIVFGS LS TTDPGDI HQTIDSLVSE KIRVKVLGLS AQVAICKELC KATNYGDESF YKILLDETHL KELFNEAVTP (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PT RIF EDTPTF CSCHSKLVYG GYFCPNCHSK VCSLPTVCPC CDLMLIL(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)KTFAEVPT TEKFRSEDCF SCQSRFP(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)SRYRCEDCK QEFCVDCDVF IHEILHNCPG CESKPV IT

UniProtKB: General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, ...UniProtKB: General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1, General transcription and DNA repair factor IIH subunit SSL1

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分子 #30: DNA repair helicase RAD25

分子名称: DNA repair helicase RAD25 / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 94.049695 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKD KALLQDTNSD IPADFVPDSV SGMFRSHDFS YLRLRPDHAS RPLWISPSDG RIILESFSPL AEQAQDFLVT I AEPISRPS ...文字列:
MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKD KALLQDTNSD IPADFVPDSV SGMFRSHDFS YLRLRPDHAS RPLWISPSDG RIILESFSPL AEQAQDFLVT I AEPISRPS HIHEYKITAY SLYAAVSVGL ETDDIISVLD RLSKVPVAES IINFIKGATI SYGKVKLVIK HNRYFVETTQ AD ILQMLLN DSVIGPLRID SDHQVQPPED VLQQQLQQTA GKPATNVNPN DVEAVFSAVI GGDNEREEED DDIDAVHSFE IAN ESVEVV KKRCQEIDYP VLEEYDFRND HRNPDLDIDL KPSTQIRPYQ EKSLSKMFGN GRARSGIIVL PCGAGKTLVG ITAA CTIKK SVIVLCTSSV SVMQWRQQFL (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)LVV ST YSMVAN(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)W GFIILDEVHV VPAAMFRRVV STIAAHAKLG LTATLVREDD KIGDLNFLIG PKLY EANWM ELSQKGHIAN VQCAEVWCPM TAEFYQEYLR ETARKRMLLY IMNPTKFQAC QFLIQYHERR GDKIIVFSDN VYALQ EYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRR NDE GFNAFFYSLV SKDTQEMYYS TKRQAFLVDQ GYAFKVITHL HGMENIPNLA YASPRERREL LQEVLLKNEE AAGIEVG DD ADNSVGRGSN GHKRFKSKAV RGEGSLSGLA GGEDMAYMEY STNKNKELKE HHPLIRKMYY KNLKK

UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General ...UniProtKB: General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2, General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2

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分子 #31: Unknown protein

分子名称: Unknown protein / タイプ: protein_or_peptide / ID: 31
詳細: Residues indicated as UNK with the chain identifier Z are part of an assembly between the clutch domains of Tfb2 and Ssl2 but could not be assigned to any of the two proteins with confidence ...詳細: Residues indicated as UNK with the chain identifier Z are part of an assembly between the clutch domains of Tfb2 and Ssl2 but could not be assigned to any of the two proteins with confidence since domain swapping/beta-strand exchange may occur. To account for this uncertainty, a theoretical chain Z was introduced for this residue range but not assigned to any of the PIC components.
コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)
分子量理論値: 3.677524 KDa
組換発現生物種: Trichoplusia ni (イラクサキンウワバ)
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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分子 #14: NONTEMPLATE DNA

分子名称: NONTEMPLATE DNA / タイプ: dna / ID: 14 / コピー数: 1 / 分類: DNA
由来(天然)生物種: synthetic construct (人工物)
分子量理論値: 32.716977 KDa
配列文字列: (DC)(DG)(DA)(DG)(DA)(DA)(DC)(DA)(DG)(DT) (DA)(DG)(DC)(DA)(DC)(DG)(DC)(DT)(DG)(DT) (DG)(DT)(DA)(DT)(DA)(DT)(DA)(DA)(DT) (DA)(DG)(DC)(DT)(DA)(DT)(DG)(DG)(DA)(DA) (DC) (DG)(DT)(DT)(DC)(DG) ...文字列:
(DC)(DG)(DA)(DG)(DA)(DA)(DC)(DA)(DG)(DT) (DA)(DG)(DC)(DA)(DC)(DG)(DC)(DT)(DG)(DT) (DG)(DT)(DA)(DT)(DA)(DT)(DA)(DA)(DT) (DA)(DG)(DC)(DT)(DA)(DT)(DG)(DG)(DA)(DA) (DC) (DG)(DT)(DT)(DC)(DG)(DA)(DT)(DT) (DC)(DA)(DC)(DC)(DT)(DC)(DC)(DG)(DA)(DT) (DG)(DT) (DG)(DT)(DG)(DT)(DT)(DG)(DT) (DA)(DC)(DA)(DT)(DA)(DC)(DA)(DT)(DA)(DA) (DA)(DA)(DA) (DT)(DA)(DT)(DC)(DA)(DT) (DA)(DG)(DC)(DA)(DC)(DA)(DA)(DC)(DT)(DG) (DC)(DG)(DC)(DT) (DG)(DT)(DG)(DT)(DC) (DA)

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分子 #18: TEMPLATE DNA

分子名称: TEMPLATE DNA / タイプ: dna / ID: 18 / コピー数: 1 / 分類: DNA
由来(天然)生物種: synthetic construct (人工物)
分子量理論値: 32.68092 KDa
配列文字列: (DT)(DG)(DA)(DC)(DA)(DC)(DA)(DG)(DC)(DG) (DC)(DA)(DG)(DT)(DT)(DG)(DT)(DG)(DC)(DT) (DA)(DT)(DG)(DA)(DT)(DA)(DT)(DT)(DT) (DT)(DT)(DA)(DT)(DG)(DT)(DA)(DT)(DG)(DT) (DA) (DC)(DA)(DA)(DC)(DA) ...文字列:
(DT)(DG)(DA)(DC)(DA)(DC)(DA)(DG)(DC)(DG) (DC)(DA)(DG)(DT)(DT)(DG)(DT)(DG)(DC)(DT) (DA)(DT)(DG)(DA)(DT)(DA)(DT)(DT)(DT) (DT)(DT)(DA)(DT)(DG)(DT)(DA)(DT)(DG)(DT) (DA) (DC)(DA)(DA)(DC)(DA)(DC)(DA)(DC) (DA)(DT)(DC)(DG)(DG)(DA)(DG)(DG)(DT)(DG) (DA)(DA) (DT)(DC)(DG)(DA)(DA)(DC)(DG) (DT)(DT)(DC)(DC)(DA)(DT)(DA)(DG)(DC)(DT) (DA)(DT)(DT) (DA)(DT)(DA)(DT)(DA)(DC) (DA)(DC)(DA)(DG)(DC)(DG)(DT)(DG)(DC)(DT) (DA)(DC)(DT)(DG) (DT)(DT)(DC)(DT)(DC) (DG)

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分子 #32: ZINC ION

分子名称: ZINC ION / タイプ: ligand / ID: 32 / コピー数: 16 / 式: ZN
分子量理論値: 65.409 Da

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分子 #33: MAGNESIUM ION

分子名称: MAGNESIUM ION / タイプ: ligand / ID: 33 / コピー数: 1 / 式: MG
分子量理論値: 24.305 Da

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分子 #34: IRON/SULFUR CLUSTER

分子名称: IRON/SULFUR CLUSTER / タイプ: ligand / ID: 34 / コピー数: 1 / 式: SF4
分子量理論値: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / 一硫化鉄

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

濃度0.70 mg/mL
緩衝液pH: 7.6
グリッドモデル: Quantifoil R1.2/1.3 / 材質: GOLD
詳細: QUANTIFOIL HOLEY GOLD GRIDS WERE GLOW-DISCHARGED BEFORE DEPOSITION OF SAMPLE.
凍結凍結剤: ETHANE / 装置: FEI VITROBOT MARK IV
詳細: GRIDS WERE BLOTTED FOR 8 S AND PLUNGE-FROZEN IN LIQUID ETHANE..

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
撮影フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k)
検出モード: COUNTING / 撮影したグリッド数: 3 / 実像数: 13000 / 平均露光時間: 10.0 sec. / 平均電子線量: 42.0 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 5.0 µm / 最小 デフォーカス（公称値）: 0.5 µm / 倍率（公称値）: 105000
試料ステージ試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
ホルダー冷却材: NITROGEN
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

初期モデルモデルのタイプ: EMDB MAP
最終 再構成想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 4.7 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 58000
初期 角度割当タイプ: PROJECTION MATCHING / ソフトウェア - 名称: RELION (ver. 2.0.4)
最終 角度割当タイプ: PROJECTION MATCHING / ソフトウェア - 名称: RELION (ver. 2.0.4)

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原子モデル構築 1

精密化空間: REAL / プロトコル: OTHER
得られたモデル

PDB-5oqj:
STRUCTURE OF YEAST TRANSCRIPTION PRE-INITIATION COMPLEX WITH TFIIH

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る