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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Structure of inactive Photosystem II associated with CAC antenna from Rhodomonas Salina | |||||||||
![]() | Composite map of PSII-CAC supercomplex from Rhodomonas Salina | |||||||||
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![]() | Photosystem II associated with CAC antenna from Rhodomonas Salina / PHOTOSYNTHESIS | |||||||||
機能・相同性 | ![]() photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem II / phosphate ion binding ...photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem II / phosphate ion binding / chloroplast thylakoid membrane / : / photosynthesis / electron transfer activity / oxidoreductase activity / protein stabilization / iron ion binding / heme binding / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.57 Å | |||||||||
![]() | Si L / Li M | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis for the distinct core-antenna assembly of cryptophyte photosystem II. 著者: Long Si / Shumeng Zhang / Xiaodong Su / Mei Li / ![]() 要旨: Photosystem II (PSII) catalyzes the light-driven charge separation and water oxidation reactions of photosynthesis. Eukaryotic PSII core is usually associated with membrane-embedded light-harvesting ...Photosystem II (PSII) catalyzes the light-driven charge separation and water oxidation reactions of photosynthesis. Eukaryotic PSII core is usually associated with membrane-embedded light-harvesting antennae, which greatly increase the absorbance cross-section of the core. The peripheral antennae in different phototrophs vary considerably in protein composition and arrangement. Photosynthetic cryptophytes possess chlorophyll a/c binding proteins (CACs) that serve as their antennae. How these CACs assemble with the PSII core remains unclear. Here, we report the 2.57-Å resolution structure of cryptophyte PSII-CAC purified from cells at nitrogen-limited stationary growth phase. We show that each monomer of the PSII homodimer contains a core complex, six chlorophyll a/c binding proteins (CACs) and a previously unseen chlorophyll-binding protein (termed CAL-II). Six CACs are arranged as a double-layered arc-shaped non-parallel belt, and two such belts attach to the dimeric core from opposite sides. The CAL-II simultaneously interacts with a number of core subunits and five CACs. The distinct organization of CACs and the presence of CAL-II may play a critical role in stabilizing the dimeric PSII-CAC complex under stress conditions. Our study provides mechanistic insights into the assembly and function of the PSII-CAC complex as well as the possible adaptation of cryptophytes in response to environmental stresses. | |||||||||
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マップデータ | ![]() | 9.1 MB | ![]() | |
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-検証レポート
文書・要旨 | ![]() | 400.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 400.2 KB | 表示 | |
XML形式データ | ![]() | 7 KB | 表示 | |
CIF形式データ | ![]() | 8.2 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 8xlpMC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Composite map of PSII-CAC supercomplex from Rhodomonas Salina | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.04003 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Inactive Photosystem II associated with chlorophyll a/c binding p...
+超分子 #1: Inactive Photosystem II associated with chlorophyll a/c binding p...
+分子 #1: Photosystem II protein D1
+分子 #2: Photosystem II CP47 reaction center protein
+分子 #3: Photosystem II CP43 reaction center protein
+分子 #4: Photosystem II D2 protein
+分子 #5: Cytochrome b559 subunit alpha
+分子 #6: Cytochrome b559 subunit beta
+分子 #7: Photosystem II reaction center protein H
+分子 #8: Photosystem II reaction center protein I
+分子 #9: Photosystem II reaction center protein K
+分子 #10: Photosystem II reaction center protein L
+分子 #11: Photosystem II protein M
+分子 #12: Photosystem II reaction center protein T
+分子 #13: Photosystem II protein W
+分子 #14: Photosystem II reaction center X protein
+分子 #15: Photosystem II reaction center protein Psb30
+分子 #16: Photosystem II reaction center protein Z
+分子 #17: NCP
+分子 #18: CAC2
+分子 #19: CAC3
+分子 #20: CAC4
+分子 #21: CAC5
+分子 #22: CAC6
+分子 #23: CAC1
+分子 #24: FE (II) ION
+分子 #25: CHLOROPHYLL A
+分子 #26: PHEOPHYTIN A
+分子 #27: 1,3,3-trimethyl-2-[(1E,3E,5E,7E,9E,11E,13E,15E,17E)-3,7,12,16-tet...
+分子 #28: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #29: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #30: BICARBONATE ION
+分子 #31: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #32: CHLORIDE ION
+分子 #33: MANGANESE (II) ION
+分子 #34: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #35: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #36: PROTOPORPHYRIN IX CONTAINING FE
+分子 #37: Chlorophyll c2
+分子 #38: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+分子 #39: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+分子 #40: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | 3D array |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN |
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撮影 | フィルム・検出器のモデル: DIRECT ELECTRON DE-16 (4k x 4k) 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.2 µm / 最小 デフォーカス(公称値): 1.2 µm |