+
Open data
-
Basic information
Entry | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | local refinement of MCM_ATP_dsDNA | |||||||||
![]() | local refinement of MCM_ATP_dsDNA | |||||||||
![]() |
| |||||||||
![]() | Helicase / Replication / HYDROLASE | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.91 Å | |||||||||
![]() | Ma J / Yi G / Ye M / MacGregor-Chatwin C / Sheng Y / Lu Y / Li M / Gilbert RJC / Zhang P | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: MCM in complex with dsDNA in presence of ATP Authors: Jianbing M / Gangshun Y / Mingda Y / Craig MGC / Yuewen S / Ying L / Ming L / Gilbert RJC / Peijun Z | |||||||||
History |
|
-
Structure visualization
Supplemental images |
---|
-
Downloads & links
-EMDB archive
Map data | ![]() | 11.1 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 12 KB 12 KB | Display Display | ![]() |
Images | ![]() | 72.9 KB | ||
Filedesc metadata | ![]() | 3.8 KB | ||
Others | ![]() ![]() | 200.6 MB 200.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | local refinement of MCM_ATP_dsDNA | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.932 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Half map: half map A of local refinement of MCM ATP dsDNA
File | emd_38125_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | half map A of local refinement of MCM_ATP_dsDNA | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: half map B of local refinement of MCM ATP dsDNA
File | emd_38125_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | half map B of local refinement of MCM_ATP_dsDNA | ||||||||||||
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : MCM homohexamer in complex with dsDNA in presence of ATP
Entire | Name: MCM homohexamer in complex with dsDNA in presence of ATP |
---|---|
Components |
|
-Supramolecule #1: MCM homohexamer in complex with dsDNA in presence of ATP
Supramolecule | Name: MCM homohexamer in complex with dsDNA in presence of ATP type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.8 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 39.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: OTHER Details: Generated using ab-initio reconstruction routine in cryoSPARC |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.91 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 126808 |
Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |