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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | local refinement of MCM_ATP_dsDNA | |||||||||
![]() | local refinement of MCM_ATP_dsDNA | |||||||||
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Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Ma J / Yi G / Ye M / MacGregor-Chatwin C / Sheng Y / Lu Y / Li M / Gilbert RJC / Zhang P | |||||||||
Funding support | ![]()
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![]() | ![]() Title: MCM in complex with dsDNA in presence of ATP Authors: Jianbing M / Gangshun Y / Mingda Y / Craig MGC / Yuewen S / Ying L / Ming L / Gilbert RJC / Peijun Z | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 11.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12 KB 12 KB | Display Display | ![]() |
Images | ![]() | 79.4 KB | ||
Filedesc metadata | ![]() | 3.8 KB | ||
Others | ![]() ![]() | 200.7 MB 200.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | local refinement of MCM_ATP_dsDNA | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.932 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: half map A of local refinement of MCM ATP dsDNA
File | emd_38123_half_map_1.map | ||||||||||||
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Annotation | half map A of local refinement of MCM_ATP_dsDNA | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: half map B of local refinement of MCM ATP dsDNA
File | emd_38123_half_map_2.map | ||||||||||||
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Annotation | half map B of local refinement of MCM_ATP_dsDNA | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : MCM homohexamer in complex with dsDNA in presence of ATP
Entire | Name: MCM homohexamer in complex with dsDNA in presence of ATP |
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Components |
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-Supramolecule #1: MCM homohexamer in complex with dsDNA in presence of ATP
Supramolecule | Name: MCM homohexamer in complex with dsDNA in presence of ATP type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 39.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: OTHER Details: Generated using ab-initio reconstruction routine in cryoSPARC |
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Initial angle assignment | Type: RANDOM ASSIGNMENT |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.57 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 126808 |