- EMDB-36866: Spiral hexameric form of the substrate-free Lon protease with a Y... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-36866
タイトル
Spiral hexameric form of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing an E613K mutation
マップデータ
Spiral hexamer
試料
複合体: Spiral hexamer of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing a E613K mutation.
タンパク質・ペプチド: Homo-hexamer of Lon protease with a Y224S mutation
ジャーナル: Nat Commun / 年: 2023 タイトル: A 5+1 assemble-to-activate mechanism of the Lon proteolytic machine. 著者: Shanshan Li / Kan-Yen Hsieh / Chiao-I Kuo / Tzu-Chi Lin / Szu-Hui Lee / Yi-Ru Chen / Chun-Hsiung Wang / Meng-Ru Ho / See-Yeun Ting / Kaiming Zhang / Chung-I Chang / 要旨: Many AAA+ (ATPases associated with diverse cellular activities) proteins function as protein or DNA remodelers by threading the substrate through the central pore of their hexameric assemblies. In ...Many AAA+ (ATPases associated with diverse cellular activities) proteins function as protein or DNA remodelers by threading the substrate through the central pore of their hexameric assemblies. In this ATP-dependent translocating state, the substrate is gripped by the pore loops of the ATPase domains arranged in a universal right-handed spiral staircase organization. However, the process by which a AAA+ protein is activated to adopt this substrate-pore-loop arrangement remains unknown. We show here, using cryo-electron microscopy (cryo-EM), that the activation process of the Lon AAA+ protease may involve a pentameric assembly and a substrate-dependent incorporation of the sixth protomer to form the substrate-pore-loop contacts seen in the translocating state. Based on the structural results, we design truncated monomeric mutants that inhibit Lon activity by binding to the native pentamer and demonstrated that expressing these monomeric mutants in Escherichia coli cells containing functional Lon elicits specific phenotypes associated with lon deficiency, including the inhibition of persister cell formation. These findings uncover a substrate-dependent assembly process for the activation of a AAA+ protein and demonstrate a targeted approach to selectively inhibit its function within cells.
全体 : Spiral hexamer of the substrate-free Lon protease with a Y224S mu...
全体
名称: Spiral hexamer of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing a E613K mutation.
要素
複合体: Spiral hexamer of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing a E613K mutation.
タンパク質・ペプチド: Homo-hexamer of Lon protease with a Y224S mutation
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超分子 #1: Spiral hexamer of the substrate-free Lon protease with a Y224S mu...
超分子
名称: Spiral hexamer of the substrate-free Lon protease with a Y224S mutation in the presence of the N-terminal-truncated monomeric mutant bearing a E613K mutation. タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
由来(天然)
生物種: Meiothermus taiwanensis (バクテリア)
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分子 #1: Homo-hexamer of Lon protease with a Y224S mutation
分子
名称: Homo-hexamer of Lon protease with a Y224S mutation / タイプ: protein_or_peptide / ID: 1 / 光学異性体: LEVO