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- EMDB-3616: TFP Encapsulin particle without symmetry imposition -

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Basic information

Database: EMDB / ID: 3616
TitleTFP Encapsulin particle without symmetry imposition
Map dataTFP Encapsulin particle without symmetry imposition
SampleTFP encapsulin:
SourceBrevibacterium linens (bacteria)
Methodsingle particle reconstruction / cryo EM / 24.2 Å resolution
AuthorsPutri RM / Allende-Ballestero C / Luque D / Klem R / Rousou AA / Liu A / Traulsen CH / Rurup WF / Koay MST / Caston JR / Cornelissen JJLM
CitationJournal: ACS Nano / Year: 2017
Title: Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake.
Authors: Rindia M Putri / Carolina Allende-Ballestero / Daniel Luque / Robin Klem / Katerina-Asteria Rousou / Aijie Liu / Christoph H-H Traulsen / W Frederik Rurup / Melissa S T Koay / José R Castón / Jeroen J L M Cornelissen
DateDeposition: Mar 3, 2017 / Header (metadata) release: Apr 5, 2017 / Map release: Dec 13, 2017 / Last update: Jan 10, 2018

Structure visualization

  • Surface view with section colored by density value
  • Surface level: 0.06
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.06
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
Supplemental images

Downloads & links


Fileemd_3616.map.gz (map file in CCP4 format, 1756 KB)
Projections & slices

Image control

AxesZ (Sec.)Y (Row.)X (Col.)
76 pix
4.32 Å/pix.
= 328.32 Å
76 pix
4.32 Å/pix.
= 328.32 Å
76 pix
4.32 Å/pix.
= 328.32 Å



Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 4.32 Å
Contour Level:0.06 (by author), 0.06 (movie #1):
Minimum - Maximum-0.037812 - 0.205297
Average (Standard dev.)0.013736431 (0.044280816)


Space Group Number1
Map Geometry
Axis orderXYZ
CellA=B=C: 328.32 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.324.324.32
M x/y/z767676
origin x/y/z0.0000.0000.000
length x/y/z328.320328.320328.320
start NX/NY/NZ
MAP C/R/S123
start NC/NR/NS-38-38-38
D min/max/mean-0.0380.2050.014

Supplemental data

Sample components

Entire TFP encapsulin

EntireName: TFP encapsulin / Details: Brevibacterium linens encapsulin loaded with TFP / Number of components: 1
MassTheoretical: 2 MDa

Component #1: cellular-component, TFP encapsulin

Cellular-componentName: TFP encapsulin / Details: Brevibacterium linens encapsulin loaded with TFP / Recombinant expression: No
MassTheoretical: 2 MDa
SourceSpecies: Brevibacterium linens (bacteria)
Source (engineered)Expression System: Escherichia coli (E. coli)

Experimental details

Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionSpecimen conc.: 1 mg/ml
Buffer solution: 20 mM Tris-Cl pH 7.5, 150 mM NH4Cl, 1 mM betamercaptoethanol
pH: 7.5
VitrificationInstrument: LEICA EM CPC / Cryogen name: ETHANE

Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 10 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 50000 X (nominal) / Cs: 2.26 mm / Imaging mode: BRIGHT FIELD
CameraDetector: FEI EAGLE (4k x 4k)

Image acquisition

Image acquisitionNumber of digital images: 128 / Sampling size: 15 microns

Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 19779
3D reconstructionAlgorithm: FOURIER SPACE / Software: RELION / Resolution: 24.2 Å / Resolution method: FSC 0.5 CUT-OFF

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