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TitleStructural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake.
Journal, issue, pagesACS Nano, Vol. 11, Issue 12, Page 12796-12804, Year 2017
Publish dateDec 26, 2017
AuthorsRindia M Putri / Carolina Allende-Ballestero / Daniel Luque / Robin Klem / Katerina-Asteria Rousou / Aijie Liu / Christoph H-H Traulsen / W Frederik Rurup / Melissa S T Koay / José R Castón / Jeroen J L M Cornelissen /
PubMed AbstractRecent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a ...Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo, resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM). Furthermore, by harnessing the confined enzyme (i.e., a peroxidase), we demonstrate the functionality of the encapsulin for an in vitro surface-immobilized catalysis in a cascade pathway with an additional enzyme, glucose oxidase. We also demonstrate the in vivo functionality of the encapsulin for cellular uptake using mammalian macrophages. Unraveling both the structure and functionality of the encapsulins allows transforming biological nanocompartments into functional systems.
External linksACS Nano / PubMed:29166561 / PubMed Central
MethodsEM (single particle)
Resolution11.4 - 28.3 Å
Structure data

EMDB-3608:
Non-loaded encapsulin particles with icosahedral symmetry
Method: EM (single particle) / Resolution: 11.4 Å

EMDB-3609:
Non-loaded encapsulin particles
Method: EM (single particle) / Resolution: 27.3 Å

EMDB-3612:
DyP Encapsulin particle with imposed icosahedral symmetry
Method: EM (single particle) / Resolution: 15.1 Å

EMDB-3613:
DyP Encapsulin particle with imposed C3 symmetry
Method: EM (single particle) / Resolution: 24.7 Å

EMDB-3614:
DyP Encapsulin particle without symmetry imposition
Method: EM (single particle) / Resolution: 28.3 Å

EMDB-3615:
TFP Encapsulin particle with imposed icosahedral symmetry
Method: EM (single particle) / Resolution: 13.5 Å

EMDB-3616:
TFP Encapsulin particle without symmetry imposition
Method: EM (single particle) / Resolution: 24.2 Å

Source
  • Brevibacterium linens (bacteria)

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