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- EMDB-3577: MTA2-RBBP7 complex -

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Basic information

Entry
Database: EMDB / ID: 3577
TitleMTA2-RBBP7 complex
SampleMTA2-RBBP7 complex
SourceHomo sapiens / human
Map data
Methodsingle particle reconstruction, at 38 Å resolution
AuthorsBrasen C / Dorosz J / Wiuf A / Boesen T / Mirza O / Gajhede M
CitationBiochim. Biophys. Acta, 2017, 1865, 531-538

Biochim. Biophys. Acta, 2017, 1865, 531-538 Yorodumi Papers
Expression, purification and characterization of the human MTA2-RBBP7 complex.
Christoffer Brasen / Jerzy Dorosz / Anders Wiuf / Thomas Boesen / Osman Mirza / Michael Gajhede

DateDeposition: Jan 17, 2017 / Header (metadata) release: Feb 15, 2017 / Map release: May 3, 2017 / Last update: May 3, 2017

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.02
  • Imaged by UCSF CHIMERA
  • Download
3D viewer


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Supplemental images

Downloads & links

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Map

Fileemd_3577.map.gz (map file in CCP4 format, 19653 KB)
Projections & slices

Image control

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AxesZ (Sec.)Y (Row.)X (Col.)
170 pix
3.15 Å/pix.
= 535.5 Å
170 pix
3.15 Å/pix.
= 535.5 Å
170 pix
3.15 Å/pix.
= 535.5 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 3.15 Å
Density
Contour Level:0.005 (by emdb), 0.02 (movie #1):
Minimum - Maximum-0.032571185 - 0.10222287
Average (Standard dev.)0.00017141107 (0.0044833473)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions170170170
Origin000
Limit169169169
Spacing170170170
CellA=B=C: 535.5 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z3.153.153.15
M x/y/z170170170
origin x/y/z0.0000.0000.000
length x/y/z535.500535.500535.500
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS170170170
D min/max/mean-0.0330.1020.000

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Supplemental data

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Sample components

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Entire MTA2-RBBP7 complex

EntireName: MTA2-RBBP7 complex / Number of components: 1

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Component #1: protein, MTA2-RBBP7 complex

ProteinName: MTA2-RBBP7 complex / Recombinant expression: No
SourceSpecies: Homo sapiens / human
Source (engineered)Expression System: Homo sapiens / human / Vector: pOPINHALO7/pOPINF / Cell of expression system: HEK293F

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Experimental details

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Sample preparation

Specimen stateparticle
Sample solutionpH: 7.5
VitrificationCryogen name: NONE

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Electron microscopy imaging

Experimental equipment
Model: Tecnai Spirit / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI SPIRIT
Electron gunElectron source: LAB6 / Accelerating voltage: 120 kV / Electron dose: 10 e/Å2 / Illumination mode: OTHER
LensCs: 2.2 mm / Imaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: TVIPS TEMCAM-F416 (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 1267
3D reconstructionResolution: 38 Å / Resolution method: FSC 0.143 CUT-OFF

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External links: The 2017 Nobel Prize in Chemistry - Press Release

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