[English] 日本語
Yorodumi
- EMDB-3542: Structure of a spliceosome remodeled for exon ligation -

+
Open data


ID or keywords:

Loading...

no data

-
Basic information

Entry
Database: EMDB / ID: 3542
TitleStructure of a spliceosome remodeled for exon ligation
SampleSaccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling
SourceSaccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /
Map dataMap of Saccharomyces cerevisiae spliceosome C-star complex generated by focussed refinement on U2 snRNP peripheral regions.
Methodsingle particle reconstruction, at 4.6 Å resolution
AuthorsFica SM / Oubridge C
CitationNature, 2017, 542, 377-380

primary. Nature, 2017, 542, 377-380 StrPapers
Structure of a spliceosome remodelled for exon ligation.
Sebastian M Fica / Chris Oubridge / Wojciech P Galej / Max E Wilkinson / Xiao-Chen Bai / Andrew J Newman / Kiyoshi Nagai

1. Science, 2015, 349, 1191-1198 StrPapers
Structural basis of pre-mRNA splicing.
Jing Hang / Ruixue Wan / Chuangye Yan / Yigong Shi

2. Nature, 2015, 523, 47-52 StrPapers
The architecture of the spliceosomal U4/U6.U5 tri-snRNP.
Thi Hoang Duong Nguyen / Wojciech P Galej / Xiao-chen Bai / Christos G Savva / Andrew J Newman / Sjors H W Scheres / Kiyoshi Nagai

3. Nature, 2016, 530, 298-302 StrPapers
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.
Thi Hoang Duong Nguyen / Wojciech P Galej / Xiao-chen Bai / Chris Oubridge / Andrew J Newman / Sjors H W Scheres / Kiyoshi Nagai

4. Nature, 2016, 537, 197-201 StrPapers
Cryo-EM structure of the spliceosome immediately after branching.
Wojciech P Galej / Max E Wilkinson / Sebastian M Fica / Chris Oubridge / Andrew J Newman / Kiyoshi Nagai

DateDeposition: Dec 19, 2016 / Header (metadata) release: Jan 11, 2017 / Map release: Jan 18, 2017 / Last update: Dec 19, 2016

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.028
  • Imaged by UCSF CHIMERA
  • Download
  • Surface view colored by radius
  • Surface level: 0.028
  • Imaged by UCSF CHIMERA
  • Download
3D viewer


View / / Stereo:
Center
Zoom
Scale
Slabnear <=> far

fix: /
Orientation
Orientation Rotation
Misc. /
Show/hide
Supplemental images

Downloads & links

-
Map

Fileemd_3542.map.gz (map file in CCP4 format, 279739 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
412 pix
1.43 Å/pix.
= 589.16 Å
412 pix
1.43 Å/pix.
= 589.16 Å
412 pix
1.43 Å/pix.
= 589.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Voxel sizeX=Y=Z: 1.43 Å
Density
Contour Level:0.028 (by author), 0.028 (movie #1):
Minimum - Maximum-0.01757239 - 0.07314288
Average (Standard dev.)0.00026389147 (0.002914196)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions412412412
Origin000
Limit411411411
Spacing412412412
CellA=B=C: 589.16 Å
Alpha=beta=gamma: 90 deg.

CCP4 map header:

modeImage stored as Reals
A/pix X/Y/Z1.431.431.43
M x/y/z412412412
origin x/y/z0.0000.0000.000
length x/y/z589.160589.160589.160
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS412412412
D min/max/mean-0.0180.0730.000

-
Supplemental data

-
Sample components

+
Entire Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-...

EntireName: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling
Details: Splicing extract was prepared from Slu7-TAPS yeast strains. An in vitro transcribed yeast UBC4 pre-mRNA substrate (with 2 x MS2 bacteriophage coat protein-binding stem loops at the 5' end and with a 2'-deoxy substitution at the 3'-splice site sequence UAG sequence (UA-2'dG) was pre-bound to an MS2-maltose binding protein fusion protein. This substrate-protein complex was added to the splicing extract. The splicing reaction proceeded through the first step but the second step was blocked by the deoxy substitution. Substrate-bound spliceosomes from the splicing extract were purified on amylose resin and eluted with maltose. Subsequently the spliceosomes were captured on streptactin resin and eluted with desthiobiotin. Purified spliceosomes were concentrated in 20 mM HEPES KOH pH 7.9, 100 mM KCl, 0.25 mM EDTA.
Number of components: 40

+
Component #1: protein, Saccharomyces cerevisiae spliceosome. Complex C just aft...

ProteinName: Saccharomyces cerevisiae spliceosome. Complex C just after Prp16-mediated remodeling
Details: Splicing extract was prepared from Slu7-TAPS yeast strains. An in vitro transcribed yeast UBC4 pre-mRNA substrate (with 2 x MS2 bacteriophage coat protein-binding stem loops at the 5' end and with a 2'-deoxy substitution at the 3'-splice site sequence UAG sequence (UA-2'dG) was pre-bound to an MS2-maltose binding protein fusion protein. This substrate-protein complex was added to the splicing extract. The splicing reaction proceeded through the first step but the second step was blocked by the deoxy substitution. Substrate-bound spliceosomes from the splicing extract were purified on amylose resin and eluted with maltose. Subsequently the spliceosomes were captured on streptactin resin and eluted with desthiobiotin. Purified spliceosomes were concentrated in 20 mM HEPES KOH pH 7.9, 100 mM KCl, 0.25 mM EDTA.
Recombinant expression: No

+
Component #2: nucleic-acid, Intron of UBC4 pre-mRNA

Nucleic-acidName: Intron of UBC4 pre-mRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUAUGUCUAA AACUAUGGCC ACGUUUCAAA UGCGUGCUUU UUUUUUAAAA CUUAUGCUCU UAUUUACUAA CAAAAUCAAC AUGCUAUUGA ACUAG
MassTheoretical: 30.183746 kDa

+
Component #3: nucleic-acid, 5'exon of UBC4 pre-mRNA

Nucleic-acidName: 5'exon of UBC4 pre-mRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GAAGUAAGUG AUCUAGAAAG
MassTheoretical: 6.518976 kDa

+
Component #4: nucleic-acid, U2 snRNA

Nucleic-acidName: U2 snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUACC UUUUAAUUUG UUACAAUACA CAUUUUUUGG CACCCAAAAU AAUAAAAUGG ACGGGAAGAG ACUUUUUAAG CAAGUUGUUU UCCGCUAAUG UCAGGUCUCA CUACUUUUUG CUGCUAUUUU UCUUCGCUCA UGGUUUCUUC AUAAGGCGUU UUUAUGAUGG UUUUUCGAAA UUGGUUUUUG AGACGACGGU UGCUCAAGGU UAUUGUUUUU GUUUUCUUCU GGUUGUUUUC UAUUUUCUUU UUUUUAGCUU UCUGUUUCUC CCUUAGUUUG GCUUUUUGCU UCAUACUCUU CCCUGUCUUU CCGAGCCGUU UAUGUCCAAC GCGGGAUUUG GUUUUUCUUU AUCGAUGGGA AGAAAUGGUG CUAUAGUAGG UUGGGAGAUA AUAUUUAUGG UAUGGGGUGC UAGUGCGGAU GGGGCGCUCU UAUUGUUGAU UUCUUCGCUC GUCUUCUUUU UCUGGUGGCG CUGCAAGAGG AAGUUUUUCG ACUUUGUUAU GAUUUUUGGU UUGCAAGGAA AGGUGUCUUA CGAUUCUUUU UUUGAUGUAA UAGGAUAAGC UUGCUUAUCC CCCAAGUAUC GGCCAAAGUU GUUGAUUUUC CUUUUGAAGU GUCCUCGGUU UGAGGGGGUG UAGGGUGGGG UUGGUCUACA AUAAGAGUGU UCCAUUGUUA ACGUGCUGGC GUCUUUUACU AUAUUUUUUU UCCCAGUUUA UUUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCUG AGAGAGUUCU GGAAGAGAAA AAAAGGAGGC AAUGGAAGGC GUUUGCUGGG AAAAGAGAAG AGCCAUGACU GCAUCUGUUG UUUCAAGGCC AGUUUUAUUA ACCGCCUAUG UCAUAGAGGC GUUUUUUUUG GAGGGAUUUG AAGAAUGCCG GCGGCAUCAA GAAACGGACU UGAUGGUUGA CGCCUGUUUU UAAAGUUAGA GACGUCGCGA CCCUCGCACU UGUGGAGUCG UUCUUGACUU UUACUUUGGU CGCUUGAUGU UUCUCUCGUC UUCCCGUUCG CUCUU
MassTheoretical: 376.267406 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #5: nucleic-acid, U6 snRNA

Nucleic-acidName: U6 snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUUU ACAAAGAGAU UUAUUUCGUU UU
MassTheoretical: 35.883176 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #6: nucleic-acid, U5 snRNA

Nucleic-acidName: U5 snRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUUC UUGCCUUUUA CCAGAACCAU CCGGGUGUUG UCUCCAUAGA AACAGGUAAA GCUGUCCGUU ACUGUGGGCU UGCCAUAUUU UUUGGAACU
MassTheoretical: 57.444875 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #7: protein, Pre-mRNA-splicing factor 8

ProteinName: Pre-mRNA-splicing factor 8 / Recombinant expression: No
MassTheoretical: 279.867469 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #8: protein, Pre-mRNA-splicing factor SNU114

ProteinName: Pre-mRNA-splicing factor SNU114 / Recombinant expression: No
MassTheoretical: 114.159039 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #9: protein, Pre-mRNA-splicing factor CWC22

ProteinName: Pre-mRNA-splicing factor CWC22 / Recombinant expression: No
MassTheoretical: 67.386062 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #10: protein, Pre-mRNA-splicing factor PRP46

ProteinName: Pre-mRNA-splicing factor PRP46 / Recombinant expression: No
MassTheoretical: 50.771289 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #11: protein, Pre-mRNA-processing protein 45

ProteinName: Pre-mRNA-processing protein 45 / Recombinant expression: No
MassTheoretical: 42.548727 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #12: protein, Pre-mRNA-splicing factor BUD31

ProteinName: Pre-mRNA-splicing factor BUD31 / Recombinant expression: No
MassTheoretical: 18.484502 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #13: protein, Pre-mRNA-splicing factor CEF1

ProteinName: Pre-mRNA-splicing factor CEF1 / Recombinant expression: No
MassTheoretical: 67.837773 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #14: protein, Pre-mRNA-splicing factor CWC15

ProteinName: Pre-mRNA-splicing factor CWC15 / Recombinant expression: No
MassTheoretical: 19.975195 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #15: protein, Pre-mRNA-splicing factor CWC21

ProteinName: Pre-mRNA-splicing factor CWC21 / Recombinant expression: No
MassTheoretical: 15.793596 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #16: protein, Pre-mRNA-splicing factor CLF1

ProteinName: Pre-mRNA-splicing factor CLF1 / Recombinant expression: No
MassTheoretical: 82.555859 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #17: protein, Pre-mRNA-splicing factor SYF1

ProteinName: Pre-mRNA-splicing factor SYF1 / Recombinant expression: No
MassTheoretical: 100.344016 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #18: protein, Pre-mRNA-splicing factor 18

ProteinName: Pre-mRNA-splicing factor 18 / Recombinant expression: No
MassTheoretical: 28.414391 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #19: protein, Pre-mRNA-splicing factor SLU7

ProteinName: Pre-mRNA-splicing factor SLU7 / Recombinant expression: No
MassTheoretical: 44.722875 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #20: protein, Pre-mRNA-splicing factor SYF2

ProteinName: Pre-mRNA-splicing factor SYF2 / Recombinant expression: No
MassTheoretical: 24.850719 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #21: protein, Small nuclear ribonucleoprotein-associated protein B

ProteinName: Small nuclear ribonucleoprotein-associated protein B / Recombinant expression: No
MassTheoretical: 22.42699 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #22: protein, Small nuclear ribonucleoprotein Sm D3

ProteinName: Small nuclear ribonucleoprotein Sm D3 / Recombinant expression: No
MassTheoretical: 11.240139 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #23: protein, Small nuclear ribonucleoprotein E

ProteinName: Small nuclear ribonucleoprotein E / Recombinant expression: No
MassTheoretical: 10.385098 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #24: protein, Small nuclear ribonucleoprotein F

ProteinName: Small nuclear ribonucleoprotein F / Recombinant expression: No
MassTheoretical: 9.669945 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #25: protein, Small nuclear ribonucleoprotein G

ProteinName: Small nuclear ribonucleoprotein G / Recombinant expression: No
MassTheoretical: 8.490809 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #26: protein, Small nuclear ribonucleoprotein Sm D1

ProteinName: Small nuclear ribonucleoprotein Sm D1 / Recombinant expression: No
MassTheoretical: 16.296798 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #27: protein, Small nuclear ribonucleoprotein Sm D2

ProteinName: Small nuclear ribonucleoprotein Sm D2 / Recombinant expression: No
MassTheoretical: 12.876066 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #28: protein, Pre-mRNA-splicing factor CWC2

ProteinName: Pre-mRNA-splicing factor CWC2 / Recombinant expression: No
MassTheoretical: 38.486562 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #29: protein, Pre-mRNA-splicing factor SLT11

ProteinName: Pre-mRNA-splicing factor SLT11 / Recombinant expression: No
MassTheoretical: 40.98859 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #30: protein, Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22

ProteinName: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
Recombinant expression: No
MassTheoretical: 130.187359 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #31: protein, Pre-mRNA-processing factor 17

ProteinName: Pre-mRNA-processing factor 17 / Recombinant expression: No
MassTheoretical: 52.129707 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #32: protein, U2 small nuclear ribonucleoprotein A'

ProteinName: U2 small nuclear ribonucleoprotein A' / Recombinant expression: No
MassTheoretical: 27.232252 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #33: protein, U2 small nuclear ribonucleoprotein B''

ProteinName: U2 small nuclear ribonucleoprotein B'' / Recombinant expression: No
MassTheoretical: 12.850944 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #34: protein, Unknown protein

ProteinName: Unknown protein / Recombinant expression: No
MassTheoretical: 5.805147 kDa
SourceSpecies: Saccharomyces cerevisiae / yeast / サッカロミセス・セレビシエ /

+
Component #35: nucleic-acid, 3'exon of UBC4 pre-mRNA bound to Prp22 helicase

Nucleic-acidName: 3'exon of UBC4 pre-mRNA bound to Prp22 helicase / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UUU
MassTheoretical: 0.87354 kDa

+
Component #36: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 3 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #37: ligand, POTASSIUM ION

LigandName: POTASSIUM ION / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 3.909805 MDa

+
Component #38: ligand, INOSITOL HEXAKISPHOSPHATE

LigandName: INOSITOL HEXAKISPHOSPHATE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.660035 kDa

+
Component #39: ligand, GUANOSINE-5'-TRIPHOSPHATE

LigandName: GUANOSINE-5'-TRIPHOSPHATE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.52318 kDa

+
Component #40: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 6 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

-
Experimental details

-
Sample preparation

Specimen stateparticle
Sample solutionSpecimen conc.: 0.3 mg/ml / Buffer solution: NP-40 is also called IGEPAL CA-630 / pH: 7.9
VitrificationInstrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %
Details: 3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 81000 X (nominal) / Imaging mode: BRIGHT FIELD / Defocus: 500 - 4500 nm / Energy filter: GIF Quantum energy filter, 20 eV slit width
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: GATAN K2 (4k x 4k)

-
Image acquisition

Image acquisitionNumber of digital images: 3596
Details: Total dose: 40 electrons/Angstrom^2 over 16 seconds. 20 movie frames collected at 1.25 frames per second.

-
Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 61107
3D reconstructionSoftware: RELION / Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF
Details: A 3D reconstruction was obtained after refinement of a subset of particles obtained by classification with a mask around the N-terminus of Syf1 and the peripheral parts of the U2 snRNP (U2 Sm ring, Msl1, Lea1).
FSC plot (resolution assessment)

+
About Yorodumi

-
News

-
Jun 16, 2017. Omokage search with filter

Omokage search with filter

  • Result of Omokage search can be filtered by keywords and the database types

Related info.: Omokage search

-
Sep 15, 2016. EM Navigator & Yorodumi renewed

EM Navigator & Yorodumi renewed

  • New versions of EM Navigator and Yorodumi started

Related info.: Changes in new EM Navigator and Yorodumi / EM Navigator (legacy version) / Yorodumi (legacy version)

+
Aug 31, 2016. New EM Navigator & Yorodumi

New EM Navigator & Yorodumi

  • In 15th Sep 2016, the development versions of EM Navigator and Yorodumi will replace the official versions.
  • Current version will continue as 'legacy version' for some time.

Related info.: Changes in new EM Navigator and Yorodumi / EM Navigator / Yorodumi / EM Navigator (legacy version) / Yorodumi (legacy version)

+
Apr 13, 2016. Omokage search got faster

Omokage search got faster

  • The computation time became ~1/2 compared to the previous version by re-optimization of data accession
  • Enjoy "shape similarity" of biomolecules, more!

Related info.: Omokage search

+
Mar 3, 2016. Presentation (PDF format) at IPR seminar on Feb 19.

Read more

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • All the functionalities will be ported from the levgacy version.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.

Related info.: Yorodumi (legacy version) / EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Yorodumi Papers / Jmol/JSmol / Changes in new EM Navigator and Yorodumi

Read more