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Yorodumi- EMDB-35271: cryo-EM map of the ACE2-B0AT1 complex bound with methionine, focu... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35271 | |||||||||
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Title | cryo-EM map of the ACE2-B0AT1 complex bound with methionine, focused refined on extracellular region | |||||||||
Map data | ||||||||||
Sample |
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Keywords | transporter / MEMBRANE PROTEIN | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.9 Å | |||||||||
Authors | Li YN / Zhang YY / Shen YP / Yan RH | |||||||||
Funding support | China, 1 items
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Citation | Journal: Cell Discov / Year: 2023 Title: Structural insight into the substrate recognition and transport mechanism of amino acid transporter complex ACE2-BAT1 and ACE2-SIT1. Authors: Yaning Li / Yiming Chen / Yuanyuan Zhang / Yaping Shen / Kangtai Xu / Yaqi Liu / Zilong Wang / Renhong Yan / | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35271.map.gz | 84.3 MB | EMDB map data format | |
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Header (meta data) | emd-35271-v30.xml emd-35271.xml | 12 KB 12 KB | Display Display | EMDB header |
Images | emd_35271.png | 46.8 KB | ||
Others | emd_35271_half_map_1.map.gz emd_35271_half_map_2.map.gz | 3.4 MB 3.4 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35271 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35271 | HTTPS FTP |
-Validation report
Summary document | emd_35271_validation.pdf.gz | 651.4 KB | Display | EMDB validaton report |
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Full document | emd_35271_full_validation.pdf.gz | 650.9 KB | Display | |
Data in XML | emd_35271_validation.xml.gz | 12.9 KB | Display | |
Data in CIF | emd_35271_validation.cif.gz | 15.2 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35271 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35271 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35271.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_35271_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35271_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : ACE2-SIT1 complex bound with proline
Entire | Name: ACE2-SIT1 complex bound with proline |
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Components |
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-Supramolecule #1: ACE2-SIT1 complex bound with proline
Supramolecule | Name: ACE2-SIT1 complex bound with proline / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 244699 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |