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- EMDB-3525: Cryo-EM structure of the spinach chloroplast ribosome reveals the... -

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Basic information

Entry
Database: EMDB / ID: EMD-3525
TitleCryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions
Map dataReconstruction of the spinach chloroplast 50S ribosomal subunit.
Sample
  • Complex: 50S subunit of the spinach chloroplast ribosome
    • RNA: x 3 types
    • Protein or peptide: x 29 types
Function / homology
Function and homology information


plastid translation / mitochondrial large ribosomal subunit / chloroplast stroma / mitochondrial translation / chloroplast / DNA-templated transcription termination / large ribosomal subunit rRNA binding / large ribosomal subunit / 5S rRNA binding / cytoplasmic translation ...plastid translation / mitochondrial large ribosomal subunit / chloroplast stroma / mitochondrial translation / chloroplast / DNA-templated transcription termination / large ribosomal subunit rRNA binding / large ribosomal subunit / 5S rRNA binding / cytoplasmic translation / cytosolic large ribosomal subunit / transferase activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding
Similarity search - Function
Ribosomal protein L6, chloroplast / 50S ribosomal protein 5, chloroplastic / Family of unknown function (DUF5323) / Ribosomal protein L32p, plant/cyanobacteria type / Ribosome recycling factor / Ribosome recycling factor domain / RRF superfamily / Ribosome recycling factor / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. ...Ribosomal protein L6, chloroplast / 50S ribosomal protein 5, chloroplastic / Family of unknown function (DUF5323) / Ribosomal protein L32p, plant/cyanobacteria type / Ribosome recycling factor / Ribosome recycling factor domain / RRF superfamily / Ribosome recycling factor / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Ribosomal protein L32p / Ribosomal protein L34 / Ribosomal protein L34 / Ribosomal protein L13, bacterial-type / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / Ribosomal protein L5, conserved site / : / Ribosomal protein L5, N-terminal / Ribosomal protein L5 signature. / Ribosomal protein L15, conserved site / Ribosomal protein L5, C-terminal / Ribosomal protein L5 / Ribosomal protein L5 domain superfamily / Ribosomal protein L2, conserved site / Ribosomal protein L2 signature. / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L5 / ribosomal L5P family C-terminus / Ribosomal protein L16p/L10e / Ribosomal protein L2, domain 3 / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L6
Similarity search - Domain/homology
Large ribosomal subunit protein uL3c / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein uL18c / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein bL28c / Large ribosomal subunit protein bL17c / Large ribosomal subunit protein bL9c / Large ribosomal subunit protein uL4c ...Large ribosomal subunit protein uL3c / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein uL18c / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein bL28c / Large ribosomal subunit protein bL17c / Large ribosomal subunit protein bL9c / Large ribosomal subunit protein uL4c / Large ribosomal subunit protein uL2cz/uL2cy / Large ribosomal subunit protein uL22c / Large ribosomal subunit protein uL14c / Large ribosomal subunit protein bL36c / Large ribosomal subunit protein uL13c / Large ribosomal subunit protein uL16c / Large ribosomal subunit protein uL15c / Large ribosomal subunit protein bL35c / Large ribosomal subunit protein bL21c / Large ribosomal subunit protein uL24c / Large ribosomal subunit protein cL37 alpha / Large ribosomal subunit protein bL20c / Large ribosomal subunit protein bL32c / Large ribosomal subunit protein bL33c / Large ribosomal subunit protein bL9c / Large ribosomal subunit protein bL27c / Large ribosomal subunit protein uL3c / Large ribosomal subunit protein uL5c / Large ribosomal subunit protein uL6c / Large ribosomal subunit protein bL17c / Large ribosomal subunit protein uL18c / Ribosome-recycling factor, chloroplastic / Large ribosomal subunit protein bL34c / Large ribosomal subunit protein bL28c / Large ribosomal subunit protein uL29c / Large ribosomal subunit protein cL38 / Large ribosomal subunit protein bL19c / Large ribosomal subunit protein uL23c
Similarity search - Component
Biological speciesSpinacia oleracea (spinach) / Spinach (spinach)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsGraf M / Arenz S / Huter P / Doenhoefer A / Novacek J / Wilson DN
Funding support Germany, 4 items
OrganizationGrant numberCountry
German Research FoundationFOR-1805 Germany
German Research FoundationGRK-1721 Germany
German Research FoundationSPP-1879 Germany
iNEXT653706
CitationJournal: Nucleic Acids Res / Year: 2017
Title: Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
Authors: Michael Graf / Stefan Arenz / Paul Huter / Alexandra Dönhöfer / Jirí Novácek / Daniel N Wilson /
Abstract: Ribosomes are the protein synthesizing machines of the cell. Recent advances in cryo-EM have led to the determination of structures from a variety of species, including bacterial 70S and eukaryotic ...Ribosomes are the protein synthesizing machines of the cell. Recent advances in cryo-EM have led to the determination of structures from a variety of species, including bacterial 70S and eukaryotic 80S ribosomes as well as mitoribosomes from eukaryotic mitochondria, however, to date high resolution structures of plastid 70S ribosomes have been lacking. Here we present a cryo-EM structure of the spinach chloroplast 70S ribosome, with an average resolution of 5.4 Å for the small 30S subunit and 3.6 Å for the large 50S ribosomal subunit. The structure reveals the location of the plastid-specific ribosomal proteins (RPs) PSRP1, PSRP4, PSRP5 and PSRP6 as well as the numerous plastid-specific extensions of the RPs. We discover many features by which the plastid-specific extensions stabilize the ribosome via establishing additional interactions with surrounding ribosomal RNA and RPs. Moreover, we identify a large conglomerate of plastid-specific protein mass adjacent to the tunnel exit site that could facilitate interaction of the chloroplast ribosome with the thylakoid membrane and the protein-targeting machinery. Comparing the Escherichia coli 70S ribosome with that of the spinach chloroplast ribosome provides detailed insight into the co-evolution of RP and rRNA.
History
DepositionDec 6, 2016-
Header (metadata) releaseDec 21, 2016-
Map releaseDec 28, 2016-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.4
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.4
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5mlc
  • Surface level: 0.4
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3525.png

Downloads & links

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Map

FileDownload / File: emd_3525.map.gz / Format: CCP4 / Size: 190.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of the spinach chloroplast 50S ribosomal subunit.
Voxel sizeX=Y=Z: 1.061 Å
Density
Contour LevelBy AUTHOR: 0.4 / Movie #1: 0.4
Minimum - Maximum-1.4556776 - 2.2175808
Average (Standard dev.)0.004995937 (±0.06810637)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions368368368
Spacing368368368
CellA=B=C: 390.448 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0611.0611.061
M x/y/z368368368
origin x/y/z0.0000.0000.000
length x/y/z390.448390.448390.448
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS368368368
D min/max/mean-1.4562.2180.005

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Supplemental data

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Sample components

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Entire : 50S subunit of the spinach chloroplast ribosome

EntireName: 50S subunit of the spinach chloroplast ribosome
Components
  • Complex: 50S subunit of the spinach chloroplast ribosome
    • RNA: 23S ribosomal RNA, chloroplastic
    • RNA: 5S ribosomal RNA, chloroplastic
    • RNA: 4.8S ribosomal RNA, chloroplastic
    • Protein or peptide: 50S ribosomal protein L2, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L3, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L4, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L5, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L6, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L9, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L13, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L14, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L15, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L16, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L17, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L18, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L19, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L20, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L21, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L22, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L23, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L24, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L27, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L28, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L29, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L32, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L33, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L34, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L35, chloroplasticRibosome
    • Protein or peptide: 50S ribosomal protein L36, chloroplasticRibosome
    • Protein or peptide: Ribosome-recycling factor, chloroplastic
    • Protein or peptide: PSRP5alpha, chloroplastic
    • Protein or peptide: PSRP6, chloroplastic

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Supramolecule #1: 50S subunit of the spinach chloroplast ribosome

SupramoleculeName: 50S subunit of the spinach chloroplast ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Spinacia oleracea (spinach) / Organelle: Chloroplast

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Macromolecule #1: 23S ribosomal RNA, chloroplastic

MacromoleculeName: 23S ribosomal RNA, chloroplastic / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 911.650438 KDa
SequenceString: UUCAAACGAG GAAAGGCUUA CGGUGGAUAC CUAGGCACCC AGAGACGAGG AAGGGCGUAU UAAUCGACGA AAUGCUUCGG GGAGUUGAA AAUAAGCAGA GAUCCGGAGA UUCCCGAAUA GGUCAACCUU UCGAACUUCU GCUGAAUCCA UGGGCAGGCA A GAGACAAC ...String:
UUCAAACGAG GAAAGGCUUA CGGUGGAUAC CUAGGCACCC AGAGACGAGG AAGGGCGUAU UAAUCGACGA AAUGCUUCGG GGAGUUGAA AAUAAGCAGA GAUCCGGAGA UUCCCGAAUA GGUCAACCUU UCGAACUUCU GCUGAAUCCA UGGGCAGGCA A GAGACAAC CUGGCGAACU GAAACAUCUU AGUAGCCAGA GGAAAAGAAA GCAAAAGCGA UUCCCGUAGU AGCGGCGAGC GA AAUGGGA GCAGCCUAAA CCGUGAAAAC GGGGUUGUGG GAGAGCAAUA CAAGCGUCGU GCUGCUAGGC GAAUCAGUGG AGU GCGGAA CCCUAGAUGG UGAAAGUCCA GUAGCCGAAA GCAUCACUAG CUUAUGCUCU GACCCGAGUA GCAUGGGGCA CGUG GAAUC CCGUGUGAAU CAGCAAGGAC CACCUUGCAA GGCUAAAUAC UCCUGGGUGA CCGAUAGCGA AGUAGUACCG UGAGG GAAG GGUGAAAAGA ACCCCCAUCG GGGAGUGAAA UAGAACAUGA AACCGUAAGC UCUCAAGCAG UGGGAGGGGG ACCAGA CCC UGACCGCGUG CCUGUUGAAG AAUGAGCCGG CGACUCAUAG GCAGUGGCUU GGUUAAGGGA ACCCACCGGA GCCGUAG CG AAAGCGAGUC UUCAUAGGGC AAUUGUCACU GCUUAUGGAC CCGAACCUGG GUGAUCUAUC CAUGACCAGG AUGAAGCU U GGGUGAAACU AAGUGGAGGU CCGAACCGAC UGAUGUUGAA GAAUCAGCGG AUGAGUUGUG GUUAGGGGUG AAAUGCCAC UCGAACCCAG AGCUAGCUGG UUCUCCCCGA AAUGCGUUGA GGCGCAGCAG UUGACUGGAC AUCUAGGGGU AAAGCACUGU UUCGGUGCG GGCCGCGAGA GCGGUACCAA AUCGAGGCAA ACUCUGAAUA CUAGAUAUGA CCUCCAAAUA ACAGGGGUCA A GGUCGGCC AGUGAGACGA UGGGGGAUAA GCUUCAUCGU CGAGAGGGAA ACAGCCCGGA UCACCAGCUA AGGCCCCUAA AU GACCGCU CAGUGAUAAA GGAGGUAGGG GUGCAGAGAC AGCCAGGAGG UUUGCCUAGA AGCAGCCACC CUUGAAAGAG UGC GUAAUA GCUCACUGAU CGAGCGCUCU UGCGCCGAAG AUGAACGGGG CUAAGCGGUC UGCCGAAGCU GUGGGAUGUA AAAA AACAU CGGUAGGGGA GCGUUCCGUG UUAGGGAGAA ACGCGUGCGU GAGCCGCGUU GGACGAAGCG GAAGCGAGAA UGUCG GCUU GAGUAACGCA AACAUUGGUG AGAAUCCAAU GCCCCGAAAA CCUAAGGGUU CCUCCGCAAG GUUCGUCCAC GGAGGG UGA GUCAGGGCCU AAGAUCAGGC CGAAAGGCGU AGUCGAUGGA CAACAGGUGA AUAUUCCUGU ACUACCCCUU GUUGGUC CC GAGGGACGGA GGAGGCUAGG UUAGCCGAAA GAUGGUUAUC GGUUCAAGGA CGCAAGGUGA CCCUGUUUUU CAGGGUAA G AAGGGGUAGA GAAAAUGCCU CGAGCCAAUG UUCGAGUACC AGGCGCUACG GCGCUGAAGU AACCGAUGCC AUACUCCCA GGAAAAGCUC GAACGACCUU CAACAAAAGG GUACCUGUAC CCGAAACCGA CACAGGUAGG UAGGUAGAGA AUACCUAGGG GCGCGAGAC AACUCUCUCU AAGGAACUCG GCAAAAUAGC CCCGUAACUU CGGGAGAAGG GGUGCCCCCU CACAAAGGGG G UCGAAGUG ACCAGGCCCG GGCGACUGUU UACCAAAAAC ACAGGUCUCC GCAAAGUCGU AAGACCAUGU AUGGGGGCUG AC GCCUGCC CAGUGCCGGA AGGUCAAGGA AGUUGGUGAC CUGAUGACAG GGGAGCCGGC GACCGAAGCC CCGGUGAACG GCG GCCGUA ACUAUAACGG UCCUAAGGUA GCGAAAUUCC UUGUCGGGUA AGUUCCGACC CGCACGAAAG GCGUAACGAU CUGG GCACU GUCUCGGAGA GAGGCUCGGU GAAAUAGACA UGUCUGUGAA GAUGCGGACU ACCUGCACCU GGACAGAAAG ACCCU AUGA AGCUUUACUG UUCCCUGGGA UUGGCUUUGG GCUUUUCCUG CGCAGCUUAG GUGGAAGGCG AAGAAGGCCC CCUUCC GGG GGGGCCCGAG CCAUCAGUGA GAUACCACUC UGGAAGAGCU AGAAUUCUAA CCUUGUGUCA GGACCUACGG GCCAAGG GA CAUUCUCAGG UAGACAGUUU CUAUGGGGCG UAGGCCUCCC AAAAGGUAAC GGAGGCGUGC AAAGGUUUCC UCGGGCCG G ACGGAGAUUG GCCCUCGAGU GCAAAGGCAG AAGGGAGCUU GACUGCAAGA CCCACCCGUC GAGCAGGGAC GAAAGUCGG CCUUAGUGAU CCGACGGUGC CGAGUGGAAG GGCCGUCGCU CAACGGAUAA AAGUUACUCU AGGGAUAACA GGCUGAUCUU CCCCAAGAG UUCACAUCGA CGGGAAGGUU UGGCACCUCG AUGUCGGCUC UUCGCCACCU GGGGCUGUAG UAUGUUCCAA G GGUUGGGC UGUUCGCCCA UUAAAGCGGU ACGUGAGCUG GGUUCAGAAC GUCGUGAGAC AGUUCGGUCC AUAUCCGGUG UG GGCGUUA GAGCAUUGAG AGGACCUUUC CCUAGUACGA GAGGACCGGG AAGGACGCAC CUCUGGUGUA CCAGUUAUCG UGC CCACGG UAAACGCUGG GUAGCCAAGU GCGGAGCGGA UAACUGCUGA AAGCAUCUAA GUAGUAAGCC CACCCCAAGA UGAG UGCUC UCCUAU

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Macromolecule #2: 5S ribosomal RNA, chloroplastic

MacromoleculeName: 5S ribosomal RNA, chloroplastic / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 39.014184 KDa
SequenceString:
UAUUCUGGUG UCCUAGGCGU AGAGGAACCA CACCAAUCCA UCCCGAACUU GGUGGUUAAA CUCUACUGCG GUGACGAUAC UGUAGGGGA GGUCCUGCGG AAAAAUAGCU CGACGCCAGG AU

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Macromolecule #3: 4.8S ribosomal RNA, chloroplastic

MacromoleculeName: 4.8S ribosomal RNA, chloroplastic / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 33.330867 KDa
SequenceString:
GAAGGUCACG GCGAGACGAG CCGUUUAUCA UUACGAUAGG UGUCAAGUGG AAGUGCAGUG AUGUAUGCAG CUGAGGCAUC CUAACAGAC CCACAGACUU GAAC

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Macromolecule #4: 50S ribosomal protein L2, chloroplastic

MacromoleculeName: 50S ribosomal protein L2, chloroplastic / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 29.853623 KDa
SequenceString: MAIHLYKTST SSTRNGAVQV KSNPRNNLIS GQRRCGKGRN ARGIITARHR GGGHKRLYRK IDFRRNEKDI YGKIVTIEYD PNRNAYICL IHYGDGEKRY ILHPRGAIIG DTIVSGTEVP IKMGNALPLT DMPLGTAIHN IEITLGRGGQ LARAAGAVAK L IAKEGKSA ...String:
MAIHLYKTST SSTRNGAVQV KSNPRNNLIS GQRRCGKGRN ARGIITARHR GGGHKRLYRK IDFRRNEKDI YGKIVTIEYD PNRNAYICL IHYGDGEKRY ILHPRGAIIG DTIVSGTEVP IKMGNALPLT DMPLGTAIHN IEITLGRGGQ LARAAGAVAK L IAKEGKSA TLKLPSGEVR LISKNCSATV GQVGNVGVNQ KRLGRAGSKR WLGKRPVVRG VVMNPVDHPH GGGEGRAPIG RK SPTTPWG YPALGRRSRK RNKYSDNFII RRRSK

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Macromolecule #5: 50S ribosomal protein L3, chloroplastic

MacromoleculeName: 50S ribosomal protein L3, chloroplastic / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 33.03475 KDa
SequenceString: MAAILPTFSI KSSSCTYSSS KRSLSSPKAV SLSSSVNGTP KVQSLRLSTS FVCSPNQIIK LKSVSPSRST QLRRAVGGLE IKMMSVDAG IGVMGTKLGM MSFFEEDGTV VPVTVIGFKE GNIVTQVKTE STDGYNAVQV GYERLRDRKL TMPERGHLNK A GVIPMRHL ...String:
MAAILPTFSI KSSSCTYSSS KRSLSSPKAV SLSSSVNGTP KVQSLRLSTS FVCSPNQIIK LKSVSPSRST QLRRAVGGLE IKMMSVDAG IGVMGTKLGM MSFFEEDGTV VPVTVIGFKE GNIVTQVKTE STDGYNAVQV GYERLRDRKL TMPERGHLNK A GVIPMRHL QEFRLVSVDD FTPSQKLLFE ELFKEGDMVD ISGTTIGKGF QGGIKRHNFK RGLMTHGSKS HRALGSIGAG TT PGHVYKG KKMPGRMGGT KTKIRKLKIM KIDTDLRVVM IKGAVPGKPG NLLRLAPAKI VGKNIPKN

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Macromolecule #6: 50S ribosomal protein L4, chloroplastic

MacromoleculeName: 50S ribosomal protein L4, chloroplastic / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 32.479617 KDa
SequenceString: MATSTSSSLS LSFFSSSLFS SKSRNFSSKP ILKLPSSSHS QTSLSLSIKS ELIPLPILNF SGEKVGETFL NLKTAPPEKA RAVVHRGLI THLQNKRRGT ASTLTRAEVR GGGRKPYPQK KTGRARRGSQ GSPLRPGGGV IFGPKPRDWT IKMNKKERRL A LSTAIASA ...String:
MATSTSSSLS LSFFSSSLFS SKSRNFSSKP ILKLPSSSHS QTSLSLSIKS ELIPLPILNF SGEKVGETFL NLKTAPPEKA RAVVHRGLI THLQNKRRGT ASTLTRAEVR GGGRKPYPQK KTGRARRGSQ GSPLRPGGGV IFGPKPRDWT IKMNKKERRL A LSTAIASA VGNSFVVEEF AENFEKPKTK DFIAAMQRWG LDPAEKSLFF LMDLVENVEK SGRNIRTLKL LTPRSLNLFD VL NAEKLVF TEGTIQYLNQ RYGVDTLEDE DEEEEEEEEG EEVDDGVEDG TPEPAE

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Macromolecule #7: 50S ribosomal protein L5, chloroplastic

MacromoleculeName: 50S ribosomal protein L5, chloroplastic / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 24.248189 KDa
SequenceString: AAGTAVFVDK AEAETINRLK TNYIEKMVPL LKEEFSYSNI LEVPKVVKIV VNCGIGDASQ NAKGLDAAIN ELALITGQRP VKTKAKTSI AGFKVREGMT LGIAVTLRGN LMYSFLDRLI NLALPRTRDF QGVNPNSFDG HGNYSVGFRE QSVFPEIKPE I VGKARGMD ...String:
AAGTAVFVDK AEAETINRLK TNYIEKMVPL LKEEFSYSNI LEVPKVVKIV VNCGIGDASQ NAKGLDAAIN ELALITGQRP VKTKAKTSI AGFKVREGMT LGIAVTLRGN LMYSFLDRLI NLALPRTRDF QGVNPNSFDG HGNYSVGFRE QSVFPEIKPE I VGKARGMD VCITTTAKTD KEAYKLLSLM GMPFREGSGP STLVRKKKLK SHHFDAKKGR RY

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Macromolecule #8: 50S ribosomal protein L6, chloroplastic

MacromoleculeName: 50S ribosomal protein L6, chloroplastic / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 24.516748 KDa
SequenceString: MSLPLPSHMK SVFLGMKVEI STSVPVTRIG FWRKSVDCKE SRIGKQPITV PANVAIAMEG QDLKVKGPLG ELSITYPREV LVEKQESGF LRVRKAVETR RANQMHGLFR TLTDNMVVGV SKGFEKKLQL VGVGYRATVE GKDLILSLGF SHPVRMAIPD E LQVKVEEN ...String:
MSLPLPSHMK SVFLGMKVEI STSVPVTRIG FWRKSVDCKE SRIGKQPITV PANVAIAMEG QDLKVKGPLG ELSITYPREV LVEKQESGF LRVRKAVETR RANQMHGLFR TLTDNMVVGV SKGFEKKLQL VGVGYRATVE GKDLILSLGF SHPVRMAIPD E LQVKVEEN TKVTVSGRDK SVVGQFAATI RSWRPPEPYK GKGVRYVDEV VRRKEGKAGK KK

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Macromolecule #9: 50S ribosomal protein L9, chloroplastic

MacromoleculeName: 50S ribosomal protein L9, chloroplastic / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 22.038904 KDa
SequenceString: MASTTSTLSL SWSNSFHSFA GAISEPQKSP ENCRVMLPIV AQKKVKKIRK IILKEDIPDL GKKGQLLDVR AGFLRNFLLP LGKAEVVTP LLLKEMKMED ERIEAEKKRV KEEAQQLARM FETVGAFKVK RKGGKGKQIF GSVTAQDLVD IIKAQLQRDV D KKVVFLPD ...String:
MASTTSTLSL SWSNSFHSFA GAISEPQKSP ENCRVMLPIV AQKKVKKIRK IILKEDIPDL GKKGQLLDVR AGFLRNFLLP LGKAEVVTP LLLKEMKMED ERIEAEKKRV KEEAQQLARM FETVGAFKVK RKGGKGKQIF GSVTAQDLVD IIKAQLQRDV D KKVVFLPD IRETGEYIAE LKLHPDVTAQ VRVTVFAN

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Macromolecule #10: 50S ribosomal protein L13, chloroplastic

MacromoleculeName: 50S ribosomal protein L13, chloroplastic / type: protein_or_peptide / ID: 10 / Details: Backbone model of the NTE. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 28.211629 KDa
SequenceString: MATMACASSL TFPSAQTQKS FFGTNVKQTP VLSFPRPTVA AAVAVSARKS TSASTKCTEE WRQLKEAVKK EFAIPHVPLD QRWMFTLEE ATGPDIWNTT WYPKSADHVP TDKKWYVVDA TDLILGRMAS TIAIHIRGKN LASYTPSVDM GAFVIVVNAD K VAVSGKKR ...String:
MATMACASSL TFPSAQTQKS FFGTNVKQTP VLSFPRPTVA AAVAVSARKS TSASTKCTEE WRQLKEAVKK EFAIPHVPLD QRWMFTLEE ATGPDIWNTT WYPKSADHVP TDKKWYVVDA TDLILGRMAS TIAIHIRGKN LASYTPSVDM GAFVIVVNAD K VAVSGKKR TQKLYRRHSG RPGGLKEETF DQLQKRIPER IIEHAVRGML PKGRLGRYLF NHLKVYKGAE HPHQAQQPID LP LRDKRIR VEK

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Macromolecule #11: 50S ribosomal protein L14, chloroplastic

MacromoleculeName: 50S ribosomal protein L14, chloroplastic / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 13.484741 KDa
SequenceString:
MIQPQTHLNV ADNSGARELM CIRIIGASNR RYARIGDVIV AVIKEAIPNT PLERSEVIRA VVVRTCKELK RDNGMIIRYD DNAAVIIDQ EGNPKGTRIF GAIARELRQK FAKIVSLAPE VL

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Macromolecule #12: 50S ribosomal protein L15, chloroplastic

MacromoleculeName: 50S ribosomal protein L15, chloroplastic / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 29.081514 KDa
SequenceString: MASLLSLSST PPSTANSNNY PSSTFKGNIN NFRINPFNFA PLKLHLRNIV KKESTRLVVV ASASSSNVSP SIGSGSETRF RLDNLGPQP GSRKKGKRKG RGHAAGQGGS CGFGMRGQKS RSGPGIMRGF EGGQMPLYRR IPKLRGIAGG MRAGLPKYVP I NLRDIEVA ...String:
MASLLSLSST PPSTANSNNY PSSTFKGNIN NFRINPFNFA PLKLHLRNIV KKESTRLVVV ASASSSNVSP SIGSGSETRF RLDNLGPQP GSRKKGKRKG RGHAAGQGGS CGFGMRGQKS RSGPGIMRGF EGGQMPLYRR IPKLRGIAGG MRAGLPKYVP I NLRDIEVA GFKEGEEVSL ESLKAKGIIN PSGRERRLPL KILGEGELST KLQIKARAFS GSAKEKLEAA GCSVTVLPGR KK YIKESVR KNLARADEYF AKKRAASASE AESA

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Macromolecule #13: 50S ribosomal protein L16, chloroplastic

MacromoleculeName: 50S ribosomal protein L16, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 15.328068 KDa
SequenceString:
MLSPKRTRFR KQHRGRMKGI SYRGNRICFG RYALQALEPA WITSRQIEAG RRAMTRNARR GGKIWVRIFP DKPVTVRPAE TRMGSGKGS PEYWVAVVKP GRILYEISGV AENIARRAVA IAASKMPIRT QFIISG

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Macromolecule #14: 50S ribosomal protein L17, chloroplastic

MacromoleculeName: 50S ribosomal protein L17, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 14.577172 KDa
SequenceString:
MIDNGGRFFA MKHGRKIHRL SRPADQRRAL LRGLTTQLLK HGRIKTTRAK ASAMRKYVDK MITLAKEGSL HKRRQALGFI YEKQIVHAL FAEVPERYGD RNGGYTRIIR TLPRRGDNAP MAYIELV

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Macromolecule #15: 50S ribosomal protein L18, chloroplastic

MacromoleculeName: 50S ribosomal protein L18, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 18.410205 KDa
SequenceString:
MAAATSLSFF HSTLASSSSS SVQQLSLPPK FVNFRPQTLP LIQAKAHTRR EDRTARHVRI RKKVEGTPER PRLCVFRSNK HLYVQVIDD TKMHTLAAAS TMQKAISENI DYSAGPTVEV AQKIGEMIAK SCLEKGITKV AFDRGGYPYH GRVKALADAA R EHGLVF

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Macromolecule #16: 50S ribosomal protein L19, chloroplastic

MacromoleculeName: 50S ribosomal protein L19, chloroplastic / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 26.121254 KDa
SequenceString: MASKVLPQAL LVIPSNHSLQ CPPLKKQLGF PIDSNRRFSL SSNCRSNLMV SRASSNLFSS NFSSIFSFPA RNSFVVRSEA EDSSDAPAE SVAVVAEEEL PVESEAEAEE RPPRQQRVKL GDIMGILNKK AVHAAEELRP VPGIRTGDIV QIRLEVPENK R RLSVYKGI ...String:
MASKVLPQAL LVIPSNHSLQ CPPLKKQLGF PIDSNRRFSL SSNCRSNLMV SRASSNLFSS NFSSIFSFPA RNSFVVRSEA EDSSDAPAE SVAVVAEEEL PVESEAEAEE RPPRQQRVKL GDIMGILNKK AVHAAEELRP VPGIRTGDIV QIRLEVPENK R RLSVYKGI VISRQNAGIH TTIRIRRIIA GVGVEIVFPL YSPNIKEIKV VSHRKVRKAR LYYLRDKLPR LSTFK

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Macromolecule #17: 50S ribosomal protein L20, chloroplastic

MacromoleculeName: 50S ribosomal protein L20, chloroplastic / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 15.725687 KDa
SequenceString:
MTRVKRGYIA RRRRKKIRFF ASSFRGAHSR LTRTIAQQKI RALVSAHRDR DRQKRDFRRL WITRINAAIR ERGVYYNYSK FIHDLYKRQ LLLNRKILAQ IAILNPNCIY MIYNEIIKKE DCKKYLEII

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Macromolecule #18: 50S ribosomal protein L21, chloroplastic

MacromoleculeName: 50S ribosomal protein L21, chloroplastic / type: protein_or_peptide / ID: 18 / Details: Backbone model of the NTE. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 28.44173 KDa
SequenceString: MASATLAFSC SSLCATLKLP QNLNPLLLNV PPLSKPFSGV VSPPSLSRLS LLPVAAKRRR FQEIPEELKA EFEEFQRPPN QKPQLSDVL PDDFQAPEPG TPEYNDIINQ FLPKKGPPPP REEIFAVVVI GSRQYIVIPG RWIYTQRLKG ATVNDKIVLN K VLLVGTKA ...String:
MASATLAFSC SSLCATLKLP QNLNPLLLNV PPLSKPFSGV VSPPSLSRLS LLPVAAKRRR FQEIPEELKA EFEEFQRPPN QKPQLSDVL PDDFQAPEPG TPEYNDIINQ FLPKKGPPPP REEIFAVVVI GSRQYIVIPG RWIYTQRLKG ATVNDKIVLN K VLLVGTKA STYIGTPIVT NAAVHAVVEE QLLDDKVIVF KYKKKKNYRR NIGHRQPITR IKITGITGYE DYPASTLEAE VE AKEEAEA EAEAEAVPV

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Macromolecule #19: 50S ribosomal protein L22, chloroplastic

MacromoleculeName: 50S ribosomal protein L22, chloroplastic / type: protein_or_peptide / ID: 19 / Details: Backbone model of the CTE. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 23.292676 KDa
SequenceString: MGFFKKKEKK AEFDVFRLYG LHHPEPGKCD EITTRGYSIS MSVDKARRVI DQIRGRSYAE TLMILELMPY RACYPIFKLI YSAAANASH NKQFNKANLI ISKAEVNKGI TLKKVKPRAR GRSYMIKRPT CHITIVLRDI THFDSYDKFL ESLTPKKLIA L LGLMSTGR ...String:
MGFFKKKEKK AEFDVFRLYG LHHPEPGKCD EITTRGYSIS MSVDKARRVI DQIRGRSYAE TLMILELMPY RACYPIFKLI YSAAANASH NKQFNKANLI ISKAEVNKGI TLKKVKPRAR GRSYMIKRPT CHITIVLRDI THFDSYDKFL ESLTPKKLIA L LGLMSTGR RRELLCGRFR ENHKIKSFLY KIALFKRYEV M

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Macromolecule #20: 50S ribosomal protein L23, chloroplastic

MacromoleculeName: 50S ribosomal protein L23, chloroplastic / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 21.832164 KDa
SequenceString: MATTAPNLHS LSSSFAFSNP SSNVSATSFT FQIPNKKAQI SCISSKKLHT QKSFNFHDAV TPMNKPSFGR DLMVAQATEA VAPTTEEAA TSQPKTSKKA KKLKYPRRIL DVYQILQSPI ITEAAIKNIA DENSLLFTVD VRADKKMIRE AISNFFGVKV R KVNTLIRP ...String:
MATTAPNLHS LSSSFAFSNP SSNVSATSFT FQIPNKKAQI SCISSKKLHT QKSFNFHDAV TPMNKPSFGR DLMVAQATEA VAPTTEEAA TSQPKTSKKA KKLKYPRRIL DVYQILQSPI ITEAAIKNIA DENSLLFTVD VRADKKMIRE AISNFFGVKV R KVNTLIRP DGTKKAYIML NKEYNASELA KKIGIFPGGN

+
Macromolecule #21: 50S ribosomal protein L24, chloroplastic

MacromoleculeName: 50S ribosomal protein L24, chloroplastic / type: protein_or_peptide / ID: 21 / Details: Backbone model of the last six amino acids. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 21.481088 KDa
SequenceString:
MAAMVLQSSF TSLSLSSNSF LGQRLFPSPT TLQVKTEGHS PCLIVMRIKR WERKDCKPNS LPKLHKRHVK VGDTVKVISG GEKGKIGEI SKIHKHNSTV IIKDLNFKTK HVKSKEEGEQ GQIIKIEAAI HSSNVMLILK EQEVADRVGH KILEDVRKVR Y LIKTGEIV DTPDRWKEIQ NKKESETAVA VAA

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Macromolecule #22: 50S ribosomal protein L27, chloroplastic

MacromoleculeName: 50S ribosomal protein L27, chloroplastic / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 21.366447 KDa
SequenceString: MAVTTSMSFN LMASFRGMSL SSSSSSSFFK GEFGPSSLRL PNKSPLSVSP FPLTIESAHK KGAGSTKNGR DSKGQRLGVK IYGDQVAKP GAIIIRQRGT KFHPGKNVGI GKDHTIFALI DGLVKFEKYG PDKKKVSVYP REIQPENPNS YRARKRENFR L QREKKKAR ...String:
MAVTTSMSFN LMASFRGMSL SSSSSSSFFK GEFGPSSLRL PNKSPLSVSP FPLTIESAHK KGAGSTKNGR DSKGQRLGVK IYGDQVAKP GAIIIRQRGT KFHPGKNVGI GKDHTIFALI DGLVKFEKYG PDKKKVSVYP REIQPENPNS YRARKRENFR L QREKKKAR REGYSFQPQL ILASAATDNA DESAVC

+
Macromolecule #23: 50S ribosomal protein L28, chloroplastic

MacromoleculeName: 50S ribosomal protein L28, chloroplastic / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 16.460488 KDa
SequenceString:
MAASGMLISN PSNVCFRKPQ FSCSLKPKAT VSELGFLTSQ LSGIQISPSP LFPIISKPIS APLKPSLQPV ARRICPFTGK KSNKANRVS HSNHKTKRLQ FVNLQYKRVW WEAGKRFVKL RLSTKALKTI EKNGLDAVAK KAGIDLRKE

+
Macromolecule #24: 50S ribosomal protein L29, chloroplastic

MacromoleculeName: 50S ribosomal protein L29, chloroplastic / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 19.083385 KDa
SequenceString:
MLAIHSLSST PCSSGLTSPP KSTLLTKSSF HGLRLPSVNL SSSLRLRVQT PPSSVVVMVK KEDELKELRT KTNEQLNEEI LQLKGELFM LRLQRSAREN FKPSDFGRMR KRVARMLTVK REREIEQGVG KRLSRKLDKA WKRSIVVRPP PSLKKLQEKA T AEAEAEKA

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Macromolecule #25: 50S ribosomal protein L32, chloroplastic

MacromoleculeName: 50S ribosomal protein L32, chloroplastic / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 6.650969 KDa
SequenceString:
MAVPKKRTSI YKKRIRKNIW KKKGYWAALK AFSLAKSLST GNSKSFFVRK ISNQTLE

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Macromolecule #26: 50S ribosomal protein L33, chloroplastic

MacromoleculeName: 50S ribosomal protein L33, chloroplastic / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 7.668121 KDa
SequenceString:
MAKGKDVRVK VILECTGCVR KSVNKGSRGV SRYITQKNRH NTPSRLELRK FCPYCYKHTI HGEIKK

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Macromolecule #27: 50S ribosomal protein L34, chloroplastic

MacromoleculeName: 50S ribosomal protein L34, chloroplastic / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 16.126633 KDa
SequenceString:
MATLSLLSTG VGAAITNRTP SASLTFITGS RTTNKRVSFN GGSARSGSLH CSFLAPSSSL SSNFSGLSLG LDLTSNTGVS TDRCRRFVV RAGKAALCLT KRSRSRKSLA RTHGFRLRMS TTSGRALLKR RRAKGRKILC TKTNPSSGKR ASP

+
Macromolecule #28: 50S ribosomal protein L35, chloroplastic

MacromoleculeName: 50S ribosomal protein L35, chloroplastic / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 17.376322 KDa
SequenceString:
MAMASATATL SFKTPSLSLS PPSTRCSAAQ GISLTHFNKQ LKSTLNLSSS SSISSSKVQP IVLKNKRIST VDSSVSTSSP SFTVFAAKG YKMKTHKASA KRFRVTGKGK IVRRRAGKQH LLAKKNTKRK NRLSKLIQVD RSDYDNVIGA LPYLKVNRKV

+
Macromolecule #29: 50S ribosomal protein L36, chloroplastic

MacromoleculeName: 50S ribosomal protein L36, chloroplastic / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 4.414462 KDa
SequenceString:
MKIRASVRPI CEKCRLIRRR GRIIVICSNP KHKQRQG

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Macromolecule #30: Ribosome-recycling factor, chloroplastic

MacromoleculeName: Ribosome-recycling factor, chloroplastic / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 30.477936 KDa
SequenceString: MAASSLSSAT SYLHSFRRRN SCVSLQGVSD MECNIARTNV SVWRSSANYV RMDCGVKKFS GKAVVVKQLQ NRAGTFRCAT MEEVEAEKS LIETNTKQRM EKTIETIRSN FNSVRTNRAS PTMLDRIEVE YYGTPVSLKS IAQISTPDAS SLLISPYDKS S LKAIEKAI ...String:
MAASSLSSAT SYLHSFRRRN SCVSLQGVSD MECNIARTNV SVWRSSANYV RMDCGVKKFS GKAVVVKQLQ NRAGTFRCAT MEEVEAEKS LIETNTKQRM EKTIETIRSN FNSVRTNRAS PTMLDRIEVE YYGTPVSLKS IAQISTPDAS SLLISPYDKS S LKAIEKAI VTSQLGVSPN NDGEVIRLSL PPLTSDRRKE LAKVVSKLAE EGKVAVRNIR RDALKSYEKL EKEKKLSEDN VK DLSADLQ KLTDEYMKKV ESIYKQKEQE LMKV

+
Macromolecule #31: PSRP5alpha, chloroplastic

MacromoleculeName: PSRP5alpha, chloroplastic / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 15.351212 KDa
SequenceString:
MALLSPLLSL SSVPPITSIA VSSSSFPIKL QNVSVALLPS FGQRLVAHGP VIAQKRGTVV AMVSAAAEET AGEDGDQSKV EEANISVQN LPLESKLQLK LEQKIKMKMA KKIRLRRTGL CAKESLRKRG AWPPSKMKKL KNV

+
Macromolecule #32: PSRP6, chloroplastic

MacromoleculeName: PSRP6, chloroplastic / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Spinach (spinach)
Molecular weightTheoretical: 12.080688 KDa
SequenceString:
MSVSAIFGAR VVTIPSVLRT SSVDGRTVKL QPSTGGSCGG GVITIECSSR PQKKGTAHHM KTRPKKTARW DIKRGPAVYP PLPPLPAEW TIVSSAVDEA DSSSSTTSSS AEIAQSA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
Component:
ConcentrationFormulaName
50.0 mMHEPES4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
100.0 mMK(OAc)Potassium acetate
25.0 mMMg(OAc)2Magnesium acetate
6.0 mMBeta-ME2-Mercaptoethanol

Details: Solutions were made fresh and filtered previous to usage.
GridModel: Quantifoil R3/3 / Material: COPPER / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Average electron dose: 2.6 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5afi

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: FREALIGN (ver. 9.11) / Number images used: 37636

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-5mlc:
Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

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