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- EMDB-34866: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota R... -
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Open data
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Basic information
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Title | Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome | |||||||||
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![]() | Sulfolobus acidocaldarius ribosome small subunit / RIBOSOME | |||||||||
Function / homology | ![]() ribonuclease P activity / tRNA 5'-leader removal / translation elongation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / ribosome biogenesis / large ribosomal subunit / regulation of translation ...ribonuclease P activity / tRNA 5'-leader removal / translation elongation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / ribosome biogenesis / large ribosomal subunit / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / GTPase activity / GTP binding / RNA binding / zinc ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.93 Å | |||||||||
![]() | Wang YH / Zhou J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome. Authors: Wang YH / Dai H / Zhang L / Wu Y / Wang J / Wang C / Xu CH / Hou H / Yang B / Zhu Y / Zhang X / Zhou J | |||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | 105.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 79.7 KB 79.7 KB | Display Display | ![]() |
Images | ![]() | 150.1 KB | ||
Filedesc metadata | ![]() | 15.3 KB | ||
Others | ![]() ![]() | 194.3 MB 194.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 15.7 KB | Display | |
Data in CIF | ![]() | 18.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8hl1MC ![]() 8hkuC ![]() 8hkvC ![]() 8hkxC ![]() 8hkyC ![]() 8hkzC ![]() 8hl2C ![]() 8hl3C ![]() 8hl4C ![]() 8hl5C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Sulfolobus acidocaldarius ribosome small sbunit
+Supramolecule #1: Sulfolobus acidocaldarius ribosome small sbunit
+Macromolecule #1: 16s rRNA (1493-MER)
+Macromolecule #2: 23s rRNA (3000-MER)
+Macromolecule #4: 5s rRNA (122-MER)
+Macromolecule #66: tRNA (76-MER)
+Macromolecule #67: mRNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3')
+Macromolecule #3: Elongation factor 2
+Macromolecule #5: 50S ribosomal protein L2
+Macromolecule #6: 50S ribosomal protein L3
+Macromolecule #7: 50S ribosomal protein L4
+Macromolecule #8: 50S ribosomal protein L5
+Macromolecule #9: 50S ribosomal protein L6
+Macromolecule #10: 50S ribosomal protein L18Ae
+Macromolecule #11: 50S ribosomal protein L10e
+Macromolecule #12: 50S ribosomal protein L13
+Macromolecule #13: 50S ribosomal protein L14e
+Macromolecule #14: 50S ribosomal protein L14
+Macromolecule #15: 50S ribosomal protein L15e
+Macromolecule #16: 50S ribosomal protein L18e
+Macromolecule #17: 50S ribosomal protein L18
+Macromolecule #18: 50S ribosomal protein L19e
+Macromolecule #19: 50S ribosomal protein L22
+Macromolecule #20: 50S ribosomal protein L23
+Macromolecule #21: 50S ribosomal protein L24e
+Macromolecule #22: 50S ribosomal protein L24
+Macromolecule #23: 50S ribosomal protein L29
+Macromolecule #24: 50S ribosomal protein L30e
+Macromolecule #25: 50S ribosomal protein L30
+Macromolecule #26: 50S ribosomal protein L31e
+Macromolecule #27: 50S ribosomal protein L32e
+Macromolecule #28: 50S ribosomal protein L34e
+Macromolecule #29: 50S ribosomal protein L37Ae
+Macromolecule #30: 50S ribosomal protein L37e
+Macromolecule #31: 50S ribosomal protein L39e
+Macromolecule #32: 50S ribosomal protein L40E
+Macromolecule #33: 50S ribosomal protein L44e
+Macromolecule #34: 50S ribosomal protein L7Ae
+Macromolecule #35: 50S ribosomal protein L15
+Macromolecule #36: 50S ribosomal protein L21e
+Macromolecule #37: DUF2280 domain-containing protein
+Macromolecule #38: Conserved protein
+Macromolecule #39: 50S ribosomal protein L47A
+Macromolecule #40: 50S ribosomal protein L1
+Macromolecule #41: 30S ribosomal protein S3
+Macromolecule #42: 30S ribosomal protein S7
+Macromolecule #43: 30S ribosomal protein S10
+Macromolecule #44: 30S ribosomal protein S13
+Macromolecule #45: 30S ribosomal protein S14 type Z
+Macromolecule #46: 30S ribosomal protein S17e
+Macromolecule #47: 30S ribosomal protein S19e
+Macromolecule #48: 30S ribosomal protein S19
+Macromolecule #49: 30S ribosomal protein S9
+Macromolecule #50: 30S ribosomal protein S28e
+Macromolecule #51: 30S ribosomal protein S27ae
+Macromolecule #52: 30S ribosomal protein S2
+Macromolecule #53: 30S ribosomal protein S4e
+Macromolecule #54: 30S ribosomal protein S4
+Macromolecule #55: 30S ribosomal protein S5
+Macromolecule #56: 30S ribosomal protein S6e
+Macromolecule #57: 30S ribosomal protein S8e
+Macromolecule #58: Small ribosomal subunit protein uS8
+Macromolecule #59: 30S ribosomal protein S11
+Macromolecule #60: 30S ribosomal protein S12
+Macromolecule #61: 30S ribosomal protein S15
+Macromolecule #62: 30S ribosomal protein S17
+Macromolecule #63: 30S ribosomal protein S24e
+Macromolecule #64: 30S ribosomal protein S27e
+Macromolecule #65: 30S ribosomal protein S3Ae
+Macromolecule #68: PHE-PHE-PHE-PHE-PHE-PHE
+Macromolecule #69: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
+Macromolecule #70: UNKNOWN LIGAND
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | 2D array |
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Sample preparation
Concentration | 5 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 26.7 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.93 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 3841 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |