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- EMDB-34861: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota R... -

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Basic information

Entry
Database: EMDB / ID: EMD-34861
TitleCryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome
Map data
Sample
  • Complex: Sulfolobus acidocaldarius ribosome small sbunit
    • RNA: x 2 types
    • Protein or peptide: x 34 types
  • Protein or peptide: x 1 types
KeywordsSulfolobus acidocaldarius ribosome small subunit / RIBOSOME
Function / homology
Function and homology information


ribonuclease P activity / tRNA 5'-leader removal / cytosolic ribosome / ribosomal large subunit biogenesis / large ribosomal subunit / ribosome biogenesis / large ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic large ribosomal subunit ...ribonuclease P activity / tRNA 5'-leader removal / cytosolic ribosome / ribosomal large subunit biogenesis / large ribosomal subunit / ribosome biogenesis / large ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytosolic large ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / RNA binding / zinc ion binding / cytoplasm
Similarity search - Function
Domain of unknown function DUF5622 / Domain of unknown function (DUF5622) / Ribosomal protein L14e / : / Ribosomal protein L40e, archaeal / Ribosomal protein L15e, archaeal / Ribosomal protein L19e, archaeal / Ribosomal protein L30, archaeal / Ribosomal protein L6P, archaea / Ribosomal protein L14P, archaeal ...Domain of unknown function DUF5622 / Domain of unknown function (DUF5622) / Ribosomal protein L14e / : / Ribosomal protein L40e, archaeal / Ribosomal protein L15e, archaeal / Ribosomal protein L19e, archaeal / Ribosomal protein L30, archaeal / Ribosomal protein L6P, archaea / Ribosomal protein L14P, archaeal / Ribosomal protein L21e, archaeal / Ribosomal protein L18e, archaea / Ribosomal protein L10e, archaea / Ribosomal protein L32e, archaeal / Ribosomal protein L3, archaeal / Ribosomal protein L4, archaea / Ribosomal protein L5, archaeal / Ribosomal protein L7Ae, archaea / Ribosomal protein L24e / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / : / metallochaperone-like domain / TRASH domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / : / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L44e / Ribosomal protein L24e, conserved site / Ribosomal protein L44 / Ribosomal protein L24e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L30e signature 1. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14 / Ribosomal protein L14, KOW motif / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L3, domain 3, archaeal type superfamily
Similarity search - Domain/homology
Uncharacterized protein / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL30 ...Uncharacterized protein / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein eL18 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL8 / Conserved protein / Large ribosomal subunit protein eL42 / Uncharacterized protein / Large ribosomal subunit protein eL14 / 50S ribosomal protein L40e / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16
Similarity search - Component
Biological speciesSulfolobus acidocaldarius DSM 639 (acidophilic)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.94 Å
AuthorsWang YH / Zhou J
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31971226 China
CitationJournal: Nucleic Acids Res / Year: 2023
Title: Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.
Authors: Ying-Hui Wang / Hong Dai / Ling Zhang / Yun Wu / Jingfen Wang / Chen Wang / Cai-Huang Xu / Hai Hou / Bing Yang / Yongqun Zhu / Xing Zhang / Jie Zhou /
Abstract: Archaeal ribosomes have many domain-specific features; however, our understanding of these structures is limited. We present 10 cryo-electron microscopy (cryo-EM) structures of the archaeal ribosome ...Archaeal ribosomes have many domain-specific features; however, our understanding of these structures is limited. We present 10 cryo-electron microscopy (cryo-EM) structures of the archaeal ribosome from crenarchaeota Sulfolobus acidocaldarius (Sac) at 2.7-5.7 Å resolution. We observed unstable conformations of H68 and h44 of ribosomal RNA (rRNA) in the subunit structures, which may interfere with subunit association. These subunit structures provided models for 12 rRNA expansion segments and 3 novel r-proteins. Furthermore, the 50S-aRF1 complex structure showed the unique domain orientation of aRF1, possibly explaining P-site transfer RNA (tRNA) release after translation termination. Sac 70S complexes were captured in seven distinct steps of the tRNA translocation reaction, confirming conserved structural features during archaeal ribosome translocation. In aEF2-engaged 70S ribosome complexes, 3D classification of cryo-EM data based on 30S head domain identified two new translocation intermediates with 30S head domain tilted 5-6° enabling its disengagement from the translocated tRNA and its release post-translocation. Additionally, we observed conformational changes to aEF2 during ribosome binding and switching from three different states. Our structural and biochemical data provide new insights into archaeal translation and ribosome translocation.
History
DepositionNov 28, 2022-
Header (metadata) releaseAug 16, 2023-
Map releaseAug 16, 2023-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_34861.png

Downloads & links

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Map

FileDownload / File: emd_34861.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
2.4 Å/pix.
x 180 pix.
= 432. Å		2.4 Å/pix.
x 180 pix.
= 432. Å		2.4 Å/pix.
x 180 pix.
= 432. Å

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Images are generated by Spider.

Voxel sizeX=Y=Z: 2.4 Å
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Contour LevelBy AUTHOR: 0.69
Minimum - Maximum-2.5888035 - 4.480949
Average (Standard dev.)-0.006015997 (±0.17824738)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions180180180
Spacing180180180
CellA=B=C: 432.00003 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_34861_half_map_1.map
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Half map: #1

Fileemd_34861_half_map_2.map
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Sample components

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Entire : Sulfolobus acidocaldarius ribosome small sbunit

EntireName: Sulfolobus acidocaldarius ribosome small sbunit
Components
  • Complex: Sulfolobus acidocaldarius ribosome small sbunit
    • RNA: 23s rRNA (2996-MER)
    • RNA: 5s rRNA (122-MER)
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L18Ae
    • Protein or peptide: 50S ribosomal protein L10e
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14e
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15e
    • Protein or peptide: 50S ribosomal protein L18e
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19e
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24e
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30e
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31e
    • Protein or peptide: 50S ribosomal protein L32e
    • Protein or peptide: 50S ribosomal protein L34e
    • Protein or peptide: 50S ribosomal protein L37Ae
    • Protein or peptide: 50S ribosomal protein L37e
    • Protein or peptide: 50S ribosomal protein L39e
    • Protein or peptide: 50S ribosomal protein L40E
    • Protein or peptide: 50S ribosomal protein L44e
    • Protein or peptide: 50S ribosomal protein L7Ae
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L21e
    • Protein or peptide: DUF2280 domain-containing protein
    • Protein or peptide: Conserved protein
  • Protein or peptide: 50S ribosomal protein L47A

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Supramolecule #1: Sulfolobus acidocaldarius ribosome small sbunit

SupramoleculeName: Sulfolobus acidocaldarius ribosome small sbunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)

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Macromolecule #1: 23s rRNA (2996-MER)

MacromoleculeName: 23s rRNA (2996-MER) / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 980.552312 KDa
SequenceString: GCCGAAGCCU CCCGGUGGAU GGCUCGGCUC GGGCACCGAA GAAGGGCGCG GCAAGCAGCG AAAUGCUCGG GUGAGGCGCA AGCAGCCGU UGACCCCGAG GUCCCCUAAU GGGAUAUCCU GCCGGGUUUC CGGCGCUCCC GGUUUAUAAC UGGGAGUGGG A ACCCCCCG ...String:
GCCGAAGCCU CCCGGUGGAU GGCUCGGCUC GGGCACCGAA GAAGGGCGCG GCAAGCAGCG AAAUGCUCGG GUGAGGCGCA AGCAGCCGU UGACCCCGAG GUCCCCUAAU GGGAUAUCCU GCCGGGUUUC CGGCGCUCCC GGUUUAUAAC UGGGAGUGGG A ACCCCCCG AACGGAAACA UCUUAGUAGG GGGAGGAAAA GAAAUCAAUU GAGAUCCCCU GAGUAGGGGC GACCGAAAGG GG GACAGCC CAAACUAAAC CUGCCGAUGA UAAGUCGGUG GGGAUGUGGU GUUACGACCU CUAGCCUGAG GUUCGACCUC GGC UUUCCU AACCUAUCUA GCCGAACUCC CCUGGAACGG GGGGCCAUAG AGGGUGAAAG CCCCGUAGGC UAAAGAUAGG UGGA AAGUG GCUAGAGGUA GAGUACCAUC CCCUGGUUUG GGGGUGGGAA GUUAGGGGAC ACGUGCCUCU AAGGCUAAAU AUGUC CCGA GACCGAUAGC AAACUAAGUA CCGUGAGGGA AAGCUGAAAA GAACCCCGGA AGGGGGAGUG CAAAGAGCCU GAAACC GGG UGGUUAUACA GGGUGUGGCU CGAAAGAAGU GAACCCUUCC GAAGGAAAGG GGCGCAAGCC CUUAGUACGA GGAAGGG CG AUCGGGGUCA CGCCUUUCGU CUUGAAACAC GGGCCGGGGA GUUCACAUCA GUGGCGAGCU UAAGGAGAUU AUCUCCGA A GGCAUAGGGA AACCAAGUGC CCGCAGCCUA GUUUCUAGGC GAGGGGCAGG GUCUGUCAGG GCCUGAAGCC ACUGAUGUG AGGCUAGAAA CCGGGCGAUC UAGUCCGGGG CAGGCUGAAG GUGGGGGAAA CCCCACUGGA GGGCCGAAUA GGGGUUCUGA CGUGCAAUU CGUUCCCUUG ACCUCGGACU AGGGGCAAAA GACCAAUCUA GCCCGGUGAU AGCUAGUUCC CCCCGAAAUG C GUCCUAGC GCAGCCUCCC UAAAGGCAGC UCGCGGGGUA GAGUGACAGA UCGGGGGCUC CAGGCGAAAG CCUGGGGCUU CC GGUCUAA CUCCGAACCC ACGAGCGCCG AAGAAGGGGG GAGUGGGUCA CUCGGCGUAA GGUUGGGUGG CAAAAGGGGA ACA ACCCAG ACCUGGGUUA AGGCCCCAAA GUCCCGGCUA AGUGCCAACG AAAAGGCGUC UCCAGCCUUA GACAGCGGGA AGGU GGGCC CAGCAGCAGC CAUCCUCUAA GGAGUGCGUA ACAGCUCACC CGCCGAGGCU GGAGGCCCUA AAGAUUGGUC GGGGC UCAA GCCGGGCGCC GAGACCCAGG AGGGGGUCUC UACUAGAGAU CCUCUGGUAG GGGGGCGCUG UGAUGGGGUA GAAGGU GGG UCGUGAGAUC CACUGGACCC GUCACAGGUG CAGAUCCCGG CGGUAGUAAC AGCGAAGGGG GGUGAGAAUC CCCCUCG CC GGAAGGGCAA GGGUUUCCCG GCAACGUUCG UCAGCCGGGA GUUAGCCGGU CCUAAGGUAG GGCCUAAUAG GUACCUAC C GAAAGGGAAA GGGGUUAACA UUCCCCUGCC UCCCGGGUAG GUGCGGUAAC GCAAGCCAGA CUCCUGACGG AUUGGGGUA GGGAGAGUAG GACCACCGUC CUACCCAAGC ACUCAAGCCC UUGGAGAGCC GUAACGGUGA GAAGAGGGCG AAGGUGUGAU GGGCCUUCC GUUAGGAGGG UUCUCCUGAU CCCUAGUCCC CAUGAAAAGG GAGUCUGGAA CGAUCCCGGG AGACCGUACC U AGAACCGA CACUGGUGCC CCUGGGUGAG AAGCCCAAGG CGUCUGAGGG GUAACCCAGG CUAGGGAACU CGGCAAAUUA GC CCCGUAA CUUCGGGAGA AGGGGUGCCU AUCGUGGUUU AACAAAGCCA CGAUAGGUCG CAGUGACCAG AGGGACCUGA CUG UUUAAU AAAAACAUAG GUCCCCGCUA GCCCGAAAGG GUGUGUACGG GGGCUAAAUC CUGGCCACUG GUGGUUGGUU AAAU CCGGG UUCAACCGGG CGAAGCCCCA CCGAAGGCCG GGGGUAACUC UGACCCUCUU AAGGUAGCCA AAUGCCUUGC CGGGU AAGU UCCGGCGCGC AUGAAUGGAU CAACGCGGUC CCUACUGUCC CAGCCUGGGG CCUCGUGAAC GCCCUGAGCC GGUGCA CAG UCCGGCAUCU CCCUACACCG AGAGAAGACC CCGUGGAGCU UCACCGCAGC CUGGCGUUGU CCCUCGGGCG UUUAUGC GU AGAGUAGGUG GGAGGGGUCG AACCUGUCCU UUCGGGGGCA GGGGACCCGA AAGUGAAACA CCACCCAUGG ACGCUCGA G GGACUAACCU CUCGAAAGAG AGGAACAACG UCAGGUGGGC GGUUCGGCUG GGGCGGCACU CCCGCGAAAA GAUAACACG GGAGCCCAAA GGUCGGCUCA GGCGGUACAG AACGCCGCCG UAGAGCGCAA GGGCAAAAGC CGGCCUGACG UGACCCUUCC AAGUACGGG GUCACGACGC GAAAGCGGGG CCUAGCGAAC GCUCGUGCCC CCACACGUGG GGGCCGGGCA UGACAGAAAA G UUACCCCG GGGAUAACAG GGUCGUCGCG GGCGAGAGCU CACAUCGACC CCGCGGUUUG CUACAUCGAU GUCGGCUCUU CC CACCCUG GAGGUGCAGC UGCCUCCAAG GGUAGGGCUG CCCGCCCGUU AAAGGGGAGC GUGAGCUGGG UUUAGACCGU CGC GAGACA GGUCGGACUC UAAGGGUAGG GAGUGCGGAC CGCUUGAGGG GAAGGAACCC CUAGUACGAG AGGAACAGGG UUCC GGGGC CUCCAGUUUA CCGGUUGUCC GGUAGGGCAA UGCCGGGCAG CCGCGCCCUG AGGGGUAACC GCUGAAAGCA UCUAA GCGG GAACCCCUCC CCUAAAAGAG GCGGUCAGGC GUUAGCCGGG GCCUUCCCCU AGAAGAGGGG GUUGAUGGGG UGGGGA UGU AAGCUCCAAG GUUGUAAGAC CGAGGAGUUU AGUCCGCCAC UCCCAAUCAG GCUCCCCCC

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Macromolecule #2: 5s rRNA (122-MER)

MacromoleculeName: 5s rRNA (122-MER) / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 39.440457 KDa
SequenceString:
UGCCCACCCG GUCAUAGUGA GCGGGUAACA CCCGGACUCG UUUCGAACCC GGAAGUUAAG CCGCUCACGU CAGAGGGGCC GUGGGAUCC GAGAGGGCCC GCAGCCUCUC UGAGCUGGGA UGG

GENBANK: GENBANK: CP000077.1

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 24.88175 KDa
SequenceString: GKKLLQQRAG RGGINFRSPS WRRVGPARYP NIEGDHKGKI IDILHNPGVT APVVKVKLDN GLQFYIPAVQ GVAVGQEISI GKNATISNG NIVEVGQLPE GTVICNVEKL KGDGGKFARA AGSYAVISGK AGNKVLIKLS SEKIVEVSQN ARATVGIIAG G GFVEKPLL ...String:
GKKLLQQRAG RGGINFRSPS WRRVGPARYP NIEGDHKGKI IDILHNPGVT APVVKVKLDN GLQFYIPAVQ GVAVGQEISI GKNATISNG NIVEVGQLPE GTVICNVEKL KGDGGKFARA AGSYAVISGK AGNKVLIKLS SEKIVEVSQN ARATVGIIAG G GFVEKPLL KAGNNYWKYR VRAVKWPVVR GVAMNAVSHP HGGGLHQSVS RPSTVSRNAP PGRKVGHIAS RRTGRR

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 38.18634 KDa
SequenceString: RKLSSPRRGS AGLRPRKRAD EILPTPKNWP LVNLKEPKLL GFIGYKAGMT HVYMIDDKPT SPNYGKEVYT PVTIVESPPI LGLALRAYH IDSKGELSVL VDYWANFEEG SLKYLKRKIT SLKVDSSKMK EKLDLIQKNL NNITYMRLLV STQPWLVPSL G KKRPEIVE ...String:
RKLSSPRRGS AGLRPRKRAD EILPTPKNWP LVNLKEPKLL GFIGYKAGMT HVYMIDDKPT SPNYGKEVYT PVTIVESPPI LGLALRAYH IDSKGELSVL VDYWANFEEG SLKYLKRKIT SLKVDSSKMK EKLDLIQKNL NNITYMRLLV STQPWLVPSL G KKRPEIVE IQIGGGSIQD QLNYGLSLLG KQIPVRDVFR EGQLTDIIGV TKGKGFQGVI KRYSVVEFPR WHKHRKGSRK IG ARGPSIS TPSYVPQPGQ LGFHRRTEYN KRIIKIGDNV NEINPAGGIV NYGLVKNTYL VIEGSVLGSR KRPLFLRYPI RPS WSPESA PKITYVNLAS QQG

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 27.357971 KDa
SequenceString: KVSVLDLKGN QLEEIELPLF FSYPVRKDLI RRVFLSEFTK SLQPKGRDPL AGKRTSALSF GINLGIARVP RVKGSGEAAL APNTVGGRL AFPPTTEKRL VEEVNLKEKK LGIISALAAT ADPNFVKARG HRFTSNNVPI ILVDDFENIS KAKEIMDILK S IGVVDDIK ...String:
KVSVLDLKGN QLEEIELPLF FSYPVRKDLI RRVFLSEFTK SLQPKGRDPL AGKRTSALSF GINLGIARVP RVKGSGEAAL APNTVGGRL AFPPTTEKRL VEEVNLKEKK LGIISALAAT ADPNFVKARG HRFTSNNVPI ILVDDFENIS KAKEIMDILK S IGVVDDIK RVKESKGVRA GKGKMRGRRY QIAKGPLIVV SNHKSPVVES ASNIPGVNVV SANLVSVIHL APGGHPGRLT IY TKSSINI LRQR

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 19.095418 KDa
SequenceString:
KENVMRRVVL DKVTVNIGVG ESGERLQKAY QLVQELTGVK PVYTKGRKSI REFGVRKGAP IGVKATLRRQ AAVEFLKKVL PAVNFRLKQ SSFDNYGNVS FGIAEHVLIP GTRYDPEIGI FGMDVAITLV RPGYRTMKRK RKKASIPRRH RVTKEEAINF M KENFNVTI

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 20.30567 KDa
SequenceString:
LKAAYIREEI QIPDKVKVSL ENNVLKVKGP KGEVIKDFSY AKGIRIQLNE GKIILETTFA DRRKKALLYS IIAHIKNMIT GTINGYRYY LKVISTHFPI SVKVSGDEVQ VSNLIGEKNI RRAKILPGVK VTVKGEDIVV EGSDIYNVAQ TAANIESSTK I VGYDRRIF SDGIYIYKKE VIG

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: 50S ribosomal protein L18Ae

MacromoleculeName: 50S ribosomal protein L18Ae / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 8.926346 KDa
SequenceString:
VKIFMVRGTA IFSASRFPTS QKFTKYVRAL NEKQAIEYIY SQLGGKNKIK RYNIHIQEIK EVKEDEITDK TIRDLA

UniProtKB: Large ribosomal subunit protein eL20

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Macromolecule #9: 50S ribosomal protein L10e

MacromoleculeName: 50S ribosomal protein L10e / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 18.670025 KDa
SequenceString:
RPGRCYRKFS GPAYTRREYI PGIPMPKITK FVMGNVNGDY DYELRLITTE KGQIRHNALE AARVIALKYL SKKLASEQNF ALVVTKYPH HVIRENKMMA FAGADRLQDG MRLSFGKPIG TAARLEKLGE LVMYVRVKKE HLEAAKEALK IASSKLPIRT K IEIS

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #10: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 15.808851 KDa
SequenceString:
VIVDATNQIL GRMSTHIAKL LKSGKKVYVV NAEKAIISGP RSRVLEGYSL LFTVKTMQNP YRQGIRRPRN PINIVKRTVR GMLPKNKLG KQIYRNLKAY IGIPKELEGK SMIRFDDADV SRLKGKYITV AELSRLLGGL K

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #11: 50S ribosomal protein L14e

MacromoleculeName: 50S ribosomal protein L14e / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 9.559101 KDa
SequenceString:
AIEIGRICVK TRGREAGKKC VIVDIIDSNF VLVTGPKEIN GVKRRRVNIL HIEPTDKKVE INKGATDQEV KQAIEQSGLT DFIKEA

UniProtKB: Large ribosomal subunit protein eL14

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Macromolecule #12: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 14.707115 KDa
SequenceString:
MNVLGSRKGF TPALQHNSSV VVADNSGAKE AVIIGIYGYR GVLRRVPFAN IADLVMVSVR KGSPDVRKQK FKAVIVRQRM PFRRPDGTW ISFEDNAVVI INPDGTPKGT EVRGPIAREA AERWPKIASV ATMVI

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #13: 50S ribosomal protein L15e

MacromoleculeName: 50S ribosomal protein L15e / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 20.179402 KDa
SequenceString:
SMYHYIEQTW QNKDWRRSIL RKRLIEWRRQ PAITRIDRPT RLNRARALGY KAKQGFVMVR VRVRRGGLNK PRPNSGRRPK RMGVYGYGP SKGYRWIAEE RAARKFPNLE VLGSYYVAED GMYKYYEVIM VDYSHPVIRS DPELKWLQDS VNRKRVFRGL T SAGQKSRG L

UniProtKB: Large ribosomal subunit protein eL15

+
Macromolecule #14: 50S ribosomal protein L18e

MacromoleculeName: 50S ribosomal protein L18e / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 12.747252 KDa
SequenceString:
TNIMLRKLIT SLKKQDKAIW VRVAEELEAP RRKRAYINIY KINRYSKAND IIVVPGKVLG VGNLDHPVTV VALSFSKPAK EKILRSGGK VMSLYKAIQE LNDFKGKTVR LMK

UniProtKB: Large ribosomal subunit protein eL18

+
Macromolecule #15: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 21.766139 KDa
SequenceString: TQGPNYRIKF RRRREGKTDY YTRYTYVINN AIRFVPRLTN KYVIVSVSKF DQKGDIMIAY AHSIELVKKY GWKGDTNNTP AAYLTGYLA GLRAVKSGVK AAVSDIGLFV PVKGGRIFAV IKGAIDAGLK VPVGDLGKLK DRVNGSHISA YAQKLKNENQ E LYNKLFSS ...String:
TQGPNYRIKF RRRREGKTDY YTRYTYVINN AIRFVPRLTN KYVIVSVSKF DQKGDIMIAY AHSIELVKKY GWKGDTNNTP AAYLTGYLA GLRAVKSGVK AAVSDIGLFV PVKGGRIFAV IKGAIDAGLK VPVGDLGKLK DRVNGSHISA YAQKLKNENQ E LYNKLFSS YIQRGLDPVL LPQHFEEVLN KIKEN

UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #16: 50S ribosomal protein L19e

MacromoleculeName: 50S ribosomal protein L19e / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 17.108916 KDa
SequenceString:
PEFQLQRRLA ADIAGVGLNN IKFNPERLEE VEEALTREDI KKLIKERAVI VNPKRGISSG RLKERKHKRR SKGEGRKHGS RKGKSGART GDKEIWINKI RKIRRYIRWL RDNNVIDKHT YRLLYKRAKG NYFKNLSDVK SYLRQ

UniProtKB: Large ribosomal subunit protein eL19

+
Macromolecule #17: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 17.360293 KDa
SequenceString:
YPILSIPEDK LAKAVIKNID ASVRDLYNVC KAIRGMNLKE AREFLNNVLE EKEALPFWRY SHGTSHRSNI SRKWKVKSGR YPKKAIKYV LKLLDNAENN ANSKGLDIDN LKIVHIAAHK GLVLKRYMTR AFGRSTRKYK YLSHIEVILG E

UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #18: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 9.261987 KDa
SequenceString:
IIKEFLATEK AVKLIESQNT LTIIVNRNTT KKDIKNEVEK LFSVKVEKIN TLITPKGEKK AYVKLKQEYK ASDVAHKLGM L

UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #19: 50S ribosomal protein L24e

MacromoleculeName: 50S ribosomal protein L24e / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 6.338608 KDa
SequenceString:
RQCSFCGKDI LPGTGLMYVR NDGSLLWFCS SKCRKSMLKL HRDPKKLKWT KSYL

UniProtKB: Large ribosomal subunit protein eL24

+
Macromolecule #20: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 14.130666 KDa
SequenceString:
MISSKPSKQR KLVYNLPNHL RYKLLTARLS EDLEKQYGIK RISIRKGDSV KLMRGSQVGY EGKVVEVDRK RGRVAIEGLT KKKADGTPV YVWVHASKVI ITKLDTGDKE RMDAIERKRK MRE

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #21: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 7.37879 KDa
SequenceString:
PLEVDELRKM DLKQLKERLN ELEMQLLKLR VESRMGTLKN TASIKNTRKD IARILTVIGE KSK

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #22: 50S ribosomal protein L30e

MacromoleculeName: 50S ribosomal protein L30e / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 10.391282 KDa
SequenceString:
FEGELKTLLR SGKVILGTRK TLKLLKTGKV KGVVVSSTLR QDLKDDIMTF SKFSDIPIYL YKGSGYELGT LCGKPFMVSV IGIVDEGES KILEF

UniProtKB: Large ribosomal subunit protein eL30

+
Macromolecule #23: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 17.819736 KDa
SequenceString:
MNELIGVIRI RGWAATPWYI QDTLSMLRLK NAFNAMIYPK DSSIQGMLNL VSSYVTWGEL NDEGLRLLVS KLEISRGKKV NEEYVKDKL NVDFQSFVES IKDGKLRLNK LDEIFSLPIR LHPPKGGFKG KVSRPFRAGG EFGYRGDKIN ELIRRM

UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #24: 50S ribosomal protein L31e

MacromoleculeName: 50S ribosomal protein L31e / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 8.935899 KDa
SequenceString:
KPEKENFEMV INFRRVIMGR KTTRTKRAIK YVRYMVKRHF GAEKVIIDPL LAKAITMNGR DKIVRRVRIA VKRIG

UniProtKB: Large ribosomal subunit protein eL31

+
Macromolecule #25: 50S ribosomal protein L32e

MacromoleculeName: 50S ribosomal protein L32e / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 14.328114 KDa
SequenceString:
KPAKKNMKSP GKAIKFLRFD WDKYYRIGRQ ERWRKPRGID NAIRLELKGY QPKVKIGYRT DKQIRGLHPS GLRPILVKSV KDLEAFAKG KQDVIIIISS TIGLRKRIEL IKKAEELGLK IANR

UniProtKB: Large ribosomal subunit protein eL32

+
Macromolecule #26: 50S ribosomal protein L34e

MacromoleculeName: 50S ribosomal protein L34e / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 8.993502 KDa
SequenceString:
SPQQRSGSFR KVFVKLPSGK STIHYERRKD NIARCGMCKK PLNGVKNNYT YKYSKTEKRP ERVYGGYLCH KCLESLI

UniProtKB: Large ribosomal subunit protein eL34

+
Macromolecule #27: 50S ribosomal protein L37Ae

MacromoleculeName: 50S ribosomal protein L37Ae / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 7.536857 KDa
SequenceString:
TGIAGRFGAR YGSSTRKKWK EVMERRYMPH QCPYCKTTGK VVRIASGIWY CRKCETKWAG LAYTP

UniProtKB: Large ribosomal subunit protein eL43

+
Macromolecule #28: 50S ribosomal protein L37e

MacromoleculeName: 50S ribosomal protein L37e / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 6.274416 KDa
SequenceString:
MKGTPSFGKM NKTPTHVRCR RCGRNSFNAR KGYCAACGFG RSKKIRRYSW QNKK

UniProtKB: Large ribosomal subunit protein eL37

+
Macromolecule #29: 50S ribosomal protein L39e

MacromoleculeName: 50S ribosomal protein L39e / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 5.861087 KDa
SequenceString:
KHKSLGKKLR LGKALKRNSP IPAWVIIKTQ AEIRFNPLRR NWRRNNLKV

UniProtKB: Large ribosomal subunit protein eL39

+
Macromolecule #30: 50S ribosomal protein L40E

MacromoleculeName: 50S ribosomal protein L40E / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 6.336725 KDa
SequenceString:
MPLTDPVKLQ IVQQRVFLKK ICRDCGALNS IRATKCRRCH SSNLRAKKKE LPAKK

UniProtKB: 50S ribosomal protein L40e

+
Macromolecule #31: 50S ribosomal protein L44e

MacromoleculeName: 50S ribosomal protein L44e / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 10.805958 KDa
SequenceString:
MKVPKVIKTY CPKCKTHTEH SVSLYKGGKR RSLAEGQRRY ERKNVGYGSK RKPEQKRFAK TTKKQTLVLK CQKCSYTIVK EGMRLKKLE LVE

UniProtKB: Large ribosomal subunit protein eL42

+
Macromolecule #32: 50S ribosomal protein L7Ae

MacromoleculeName: 50S ribosomal protein L7Ae / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 13.351481 KDa
SequenceString:
PSYVKFEVPQ ELADKVLEAV KKAKDSGKIK KGTNETTKAV ERSQAKLVVI AEDVQPEEIV AHLPLLCEEK KIPYVYVPSK KSLGEACGL QVAAASVALM DPGEAKDLVD EIVKRVNEIK GKSS

UniProtKB: Large ribosomal subunit protein eL8

+
Macromolecule #33: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 16.476072 KDa
SequenceString:
MVVRKEKKSR KYRGYRTHGW GTKGQHRDRG AQGGRQIGMH KEKWSWTVKF GEGWYGKHGF RNPTSKLVNA IGLRKLQEYI DNDKIKIEE ENGKKVVDLA KYGYDKLLGG GNLRLPLVIK VAKATEKAKE RVKEIGGEII LTSSE

UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #34: 50S ribosomal protein L21e

MacromoleculeName: 50S ribosomal protein L21e / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 11.15529 KDa
SequenceString:
VARSKGYRSK TRKLLEKKVR EKGAIPKLSL LMHDYSQGEY VVVKINPSIH KGMPHRRYHG KVGLVVGKRG KAYEVKVNIG DKERVLIVR PEHLIPFN

UniProtKB: Large ribosomal subunit protein eL21

+
Macromolecule #35: DUF2280 domain-containing protein

MacromoleculeName: DUF2280 domain-containing protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 11.662535 KDa
SequenceString:
MKVVKYVLTA KIDYDGKEIE IKRSFRHRKN IFEFLKNADS NLKGALSEYA KQNKVKSSDL PLNEFITCGE IEEEQGVMRT ECKGKEKVS VKIEVQTQKI AK

UniProtKB: Uncharacterized protein

+
Macromolecule #36: Conserved protein

MacromoleculeName: Conserved protein / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 8.320918 KDa
SequenceString:
MLKHQKYVYV DIGDQKKFLK VRFLKSRSEN NNPEAYVILD KISIKLPRNA KVIKYEDLPA EVKDKLKLKL

UniProtKB: Conserved protein

+
Macromolecule #37: 50S ribosomal protein L47A

MacromoleculeName: 50S ribosomal protein L47A / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sulfolobus acidocaldarius DSM 639 (acidophilic)
Molecular weightTheoretical: 9.276666 KDa
SequenceString:
MKISNVESKY SMKVRGQELL IEEARNEKGE KIIIFQSLTS AKLLNNEEWK ENTTDAKNVE KREDLPQDVR KITGKILSLI

UniProtKB: Uncharacterized protein

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation state2D array

-
Sample preparation

Concentration5 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 26.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v51

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 617754
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE

+
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