EMDB-33741: The structure of INTAC-PEC complex

Basic information

Entry

Database: EMDB / ID: EMD-33741

• Title: The structure of INTAC-PEC complex
• Map data: the overall map of INATC-PEC

Sample

• Complex: INTAC-PEC
  • Complex: INTAC
    • Protein or peptide: x 19 types
  • Complex: DNA-directed RNA polymerase II subunit
    • Protein or peptide: x 11 types
  • Complex: DNA/RNA
    • DNA: x 2 types
    • RNA: x 1 types
• Protein or peptide: x 1 types
• Ligand: x 3 types

• Keywords: INTAC-PEC / Transcription termination / Endonuclease / Phosphatase / TRANSCRIPTION

Function / homology

Function:

U2 snRNA 3'-end processing / NELF complex / meiotic spindle elongation / INTAC complex / Integration of energy metabolism / microfibril binding / NTRK3 as a dependence receptor / PP2A-mediated dephosphorylation of key metabolic factors / snRNA 3'-end processing / RNA polymerase II transcription initiation surveillance / MASTL Facilitates Mitotic Progression / mitotic sister chromatid separation / negative regulation of DNA-templated transcription, elongation / protein phosphatase type 2A complex / protein serine/threonine phosphatase complex / regulation of meiotic cell cycle process involved in oocyte maturation / peptidyl-threonine dephosphorylation / meiotic sister chromatid cohesion, centromeric / snRNA processing / peptidyl-serine dephosphorylation / histone H2AXS139 phosphatase activity / RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity / RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity / RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity / MAP kinase serine/threonine phosphatase activity / myosin phosphatase activity / FAR/SIN/STRIPAK complex / : / Regulation of glycolysis by fructose 2,6-bisphosphate metabolism / DSIF complex / Inhibition of replication initiation of damaged DNA by RB1/E2F1 / female meiotic nuclear division / calmodulin-dependent protein phosphatase activity / RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity / regulation of transcription elongation by RNA polymerase II / protein phosphatase regulator activity / GABA receptor binding / integrator complex / APC truncation mutants have impaired AXIN binding / AXIN missense mutants destabilize the destruction complex / Truncations of AMER1 destabilize the destruction complex / protein antigen binding / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / mRNA Splicing - Major Pathway / ERKs are inactivated / Initiation of Nuclear Envelope (NE) Reformation / negative regulation of stem cell differentiation / Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / positive regulation of extrinsic apoptotic signaling pathway in absence of ligand / Beta-catenin phosphorylation cascade / Signaling by GSK3beta mutants / CTNNB1 S33 mutants aren't phosphorylated / CTNNB1 S37 mutants aren't phosphorylated / CTNNB1 S45 mutants aren't phosphorylated / CTNNB1 T41 mutants aren't phosphorylated / Co-stimulation by CD28 / regulation of growth / positive regulation of DNA-templated transcription, elongation / Disassembly of the destruction complex and recruitment of AXIN to the membrane / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / MicroRNA (miRNA) biogenesis / negative regulation of epithelial to mesenchymal transition / inner cell mass cell proliferation / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / Co-inhibition by CTLA4 / Platelet sensitization by LDL / transcription elongation-coupled chromatin remodeling / protein-serine/threonine phosphatase / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / negative regulation of glycolytic process through fructose-6-phosphate / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / positive regulation of NLRP3 inflammasome complex assembly / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape

Domain/homology:

Domain of unknown function DUF3677 / Integrator complex subunit 2, metazoa / Integrator complex subunit 2 / Integrator complex subunit 7 / Integrator complex subunit 1 / : / : / : / : / : / : / Integrator complex subunit 1, RP2B-binding domain / Integrator complex subunit 2 / Integrator complex subunit 1, R3 domain / Integrator complex subunit 1, R4 domain / Integrator complex subunit 1, INTS2-binding domain / Integrator complex subunit 7, C-terminal domain / Integrator complex subunit 7, N-terminal / Integrator complex subunit 7 helical bundle / INTS6 beta-barrel domain / INTS6/SAGE1/DDX26B/CT45, C-terminal / : / INTS6/SAGE1/DDX26B/CT45 C-terminus / Integrator complex subunit 5, C-terminal / Integrator complex subunit 5, N-terminal / Integrator complex subunit 8 / Integrator complex subunit 5 / Integrator complex subunit 5 N-terminus / Integrator complex subunit 5 C-terminus / Negative elongation factor E / Negative elongation factor E, RNA recognition motif / Cofactor of BRCA1 / : / Cofactor of BRCA1 (COBRA1) / INTS4 8 helical bundle domain / Integrator subunit 4 family C-terminal Ig-like domain / : / : / NELF-A N-terminal domain / TH1 protein / TH1 protein / Sister chromatid cohesion protein PDS5 protein / Integrator complex subunit 9 / Hepatitis delta antigen (HDAg) domain / : / Integrator IntS9, C-terminal domain / Hepatitis delta antigen (HDAg) domain profile. / Integrator complex subunit 11, MBL-fold / : / Integrator IntS11, C-terminal domain / Metallo-beta-lactamase superfamily domain / : / : / : / Beta-Casp domain / Beta-Casp domain / Beta-Casp domain / Spt5, KOW domain repeat 6 / Transcription elongation factor SPT5, seventh KOW domain / Transcription elongation factor SPT5, sixth KOW domain / Zn-dependent metallo-hydrolase, RNA specificity domain / Zn-dependent metallo-hydrolase RNA specificity domain / Transcription initiation Spt4 / Spt4 superfamily / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Transcription elongation factor SPT5, second KOW domain / Transcription elongation factor SPT5, fifth KOW domain / Transcription elongation factor SPT5, KOWx domain / Transcription elongation factor SPT5, KOW1 domain / Transcription elongation factor SPT5, fourth KOW domain / HEAT repeat / Transcription elongation factor Spt5, eukaryote / Spt5 transcription elongation factor, N-terminal / Spt5, KOW domain repeat 2 / Spt5, KOW domain repeat 3 / Spt5, KOW domain repeat 5 / Spt5 transcription elongation factor, acidic N-terminal / NGN domain, eukaryotic / Spt5, KOW domain repeat 1 / Spt5, KOW domain repeat 4 / HEAT repeat / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / : / PPP2R1A-like HEAT repeat / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / HEAT repeat profile. / HEAT, type 2 / von Willebrand factor type A domain / HEAT repeats / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily

Component:

DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase subunit beta / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / Transcription elongation factor SPT5 / Negative elongation factor E / DNA-directed RNA polymerase II subunit RPB1 / Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform / DNA-directed RNA polymerase II subunit RPB9 / Transcription elongation factor SPT4 / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform / Integrator complex subunit 11 / Integrator complex subunit 5 / Integrator complex subunit 8 / Negative elongation factor C/D / Integrator complex subunit 1 / Negative elongation factor B / Integrator complex subunit 4 / Integrator complex subunit 2 / Negative elongation factor A / Integrator complex subunit 9 / Integrator complex subunit 7 / Integrator complex subunit 6

• Biological species: Homo sapiens (human) / Sus scrofa (pig) / synthetic construct (others)
• Method: single particle reconstruction / cryo EM / Resolution: 4.18 Å
• Authors: Zheng H / Jin Q / Wang X / Qi Y / Liu W / Ren Y / Zhao D / Chen FX / Cheng J / Chen X / Xu Y

Funding support

China, 6 items

Organization	Grant number	Country
Other government	the National key R&D program of China(2016YFA0500700)	China
Other government	the National Natural Science Foundation of China(32030055, 31830107, 31821002)	China
Other government	the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)	China
Other government	Shanghai Municipal Science and Technology Commission(19JC1411500)	China
Other government	the National Ten-Thousand Talent Program	China
Other government	the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB08000000)	China

• Citation: Journal: Protein Cell / Year: 2023; Title: Structural basis of INTAC-regulated transcription.; Authors: Hai Zheng / Qianwei Jin / Xinxin Wang / Yilun Qi / Weida Liu / Yulei Ren / Dan Zhao / Fei Xavier Chen / Jingdong Cheng / Xizi Chen / Yanhui Xu /

History

Deposition	Jul 2, 2022	-
Header (metadata) release	Mar 15, 2023	-
Map release	Mar 15, 2023	-
Update	Sep 27, 2023	-
Current status	Sep 27, 2023	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_33741.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: the overall map of INATC-PEC
• Voxel size: X=Y=Z: 1.054 Å

Density

Contour Level	By AUTHOR: 0.015
Minimum - Maximum	-0.08051763 - 0.12395028
Average (Standard dev.)	0.00017871418 (±0.0037391994)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 442.68002 Å α=β=γ: 90.0 °

Supplemental data

Additional map: #6

• File: emd_33741_additional_1.map

Additional map: #5

• File: emd_33741_additional_2.map

Additional map: #4

• File: emd_33741_additional_3.map

Additional map: #3

• File: emd_33741_additional_4.map

Additional map: #2

• File: emd_33741_additional_5.map

Additional map: #1

• File: emd_33741_additional_6.map

Half map: #2

• File: emd_33741_half_map_1.map

Half map: #1

• File: emd_33741_half_map_2.map

Sample components

Entire : INTAC-PEC

• Entire: Name: INTAC-PEC

Components

• Complex: INTAC-PEC
  • Complex: INTAC
    • Protein or peptide: Integrator complex subunit 1
    • Protein or peptide: Integrator complex subunit 2
    • Protein or peptide: Integrator complex subunit 4
    • Protein or peptide: Integrator complex subunit 5
    • Protein or peptide: Integrator complex subunit 6
    • Protein or peptide: Integrator complex subunit 7
    • Protein or peptide: Integrator complex subunit 8
    • Protein or peptide: Integrator complex subunit 11
    • Protein or peptide: Unknown2
    • Protein or peptide: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
    • Protein or peptide: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
    • Protein or peptide: Unknown
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1,DNA-directed RNA polymerase II subunit RPB1, CTD1, CTD2, CTD3, CTD4
    • Protein or peptide: Negative elongation factor A
    • Protein or peptide: Negative elongation factor B
    • Protein or peptide: Negative elongation factor C/D
    • Protein or peptide: Negative elongation factor E
    • Protein or peptide: Transcription elongation factor SPT4
    • Protein or peptide: Transcription elongation factor SPT5
  • Complex: DNA-directed RNA polymerase II subunit
    • Protein or peptide: DNA-directed RNA polymerase subunit beta
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit E
    • Protein or peptide: DNA-directed RNA polymerase II subunit F
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    • Protein or peptide: RNA_pol_L_2 domain-containing protein
    • Protein or peptide: RPB12
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
  • Complex: DNA/RNA
    • DNA: non-template DNA
    • RNA: RNA
    • DNA: template DNA
• Protein or peptide: Integrator complex subunit 9
• Ligand: ZINC ION
• Ligand: MANGANESE (II) ION
• Ligand: MAGNESIUM ION

Supramolecule #1: INTAC-PEC

• Supramolecule: Name: INTAC-PEC / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#7, #9-#34
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #2: INTAC

• Supramolecule: Name: INTAC / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#7, #9-#14, #27-#32
• Source (natural): Organism: Sus scrofa (pig)

Supramolecule #3: DNA-directed RNA polymerase II subunit

• Supramolecule: Name: DNA-directed RNA polymerase II subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #15-#23, #33-#34

Supramolecule #4: DNA/RNA

• Supramolecule: Name: DNA/RNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #24-#26

Macromolecule #1: Integrator complex subunit 1

• Macromolecule: Name: Integrator complex subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 244.574922 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MNRAKPTTVR RPSAAAKPSG HPPPGDFIAL GSKGQANESK TASTLLKPAP SGLPSERKRD AAAALSSASA LTGLTKRPKL SSTPPLSAL GRLAEAAVAE KRAISPSIKE PSVVPIEVLP TVLLDEIEAA ELEGNDDRIE GVLCGAVKQL KVTRAKPDST L YLSLMYLA KIKPNIFATE GVIEALCSLL RRDASINFKA KGNSLVSVLA CNLLMAAYEE DENWPEIFVK VYIEDSLGER IW VDSPHCK TFVDNIQTAF NTRMPPRSVL LQGEAGRVAG DLGAGSSPHP SLTEEEDSQT ELLIAEEKLS PEQEGQLMPR YEE LAESVE EYVLDMLRDQ LNRRQPIDNV SRNLLRLLTS TCGYKEVRLL AVQKLEMWLQ NPKLTRPAQD LLMSVCMNCN THGS EDMDV ISHLIKIRLK PKVLLNHFML CIRELLSAHK DNLGTTIKLV IFNELSSARN PNNMQVLYTA LQHSSELAPK FLAMV FQDL LTNKDDYLRA SRALLREIIK QTKHEINFQA FCLGLMQERK EPQYLEMEFK ERFVVHITDV LAVSMMLGIT AQVKEA GIA WDKGEKRNLE VLRSFQNQIA AIQRDAVWWL HTVVPSISKL APKDYVHCLH KVLFTEQPET YYKWDNWPPE SDRNFFL RL CSEVPILEDT LMRILVIGLS RELPLGPADA MELADHLVKR AAAVQADDVE VLKVGRTQLI DAVLNLCTYH HPENIQLP P GYQPPNLAIS TLYWKAWPLL LVVAAFNPEN IGLAAWEEYP TLKMLMEMVM TNNYSYPPCT LTDEETRTEM LNRELQTAQ REKQEILAFE GHLAAASTKQ TITESSSLLL SQLTSLDPQG PPRRPPPHIL DQVKSLNQSL RLGHLLCRSR NPDFLLHIIQ RQASSQSMP WLADLVQSSE GSLDVLPVQC LCEFLLHDAV DDAASGEEDD EGESKEQKAK KRQRQQKQRQ LLGRLQDLLL G PKADEQTT CEVLDYFLRR LGSSQVASRV LAMKGLSLVL SEGSLRDGEE KEPPMEEDVG DTDVLQGYQW LLRDLPRLPL FD SVRSTTA LALQQAIHME TDPQTISAYL IYLSQHTPVE EQAQHSDLAL DVARLVVERS TIMSHLFSKL SPSAASDAVL SAL LSIFSR YVRRMRQSKE GEEVYSWSES QDQVFLRWSS GETATMHILV VHAMVILLTL GPPRADDSEF QALLDIWFPE EKPL PTAFL VDTSEEALLL PDWLKLRMIR SEVLRLVDAA LQDLEPQQLL LFVQSFGIPV SSMSKLLQFL DQAVAHDPQT LEQNI MDKN YMAHLVEVQH ERGASGGQTF HSLLTASLPP RRDSTEAPKP KSSPEQPIGQ GRIRVGTQLR VLGPEDDLAG MFLQIF PLS PDPRWQSSSP RPVALALQQA LGQELARVVQ GSPEVPGITV RVLQALATLL SSPHGGALVM SMHRSHFLAC PLLRQLC QY QRCVPQDTGF SSLFLKVLLQ MLQWLDSPGV EGGPLRAQLR MLASQASAGR RLSDVRGGLL RLAEALAFRQ DLEVVSST V RAVIATLRSG EQCSVEPDLI SKVLQGLIEV RSPHLEELLT AFFSATADAA SPFPACKPVV VVSSLLLQEE EPLAGGKPG ADGGSLEAVR LGPSSGLLVD WLEMLDPEVV SSCPDLQLRL LFSRRKGKGQ AQVPSFRPYL LTLFTHQSSW PTLHQCIRVL LGKSREQRF DPSASLDFLW ACIHVPRIWQ GRDQRTPQKR REELVLRVQG PELISLVELI LAEAETRSQD GDTAACSLIQ A RLPLLLSC CCGDDESVRK VTEHLSGCIQ QWGDSVLGRR CRDLLLQLYL QRPELRVPVP EVLLHSEGAA SSSVCKLDGL IH RFITLLA DTSDSRALEN RGADASMACR KLAVAHPLLL LRHLPMIAAL LHGRTHLNFQ EFRQQNHLSC FLHVLGLLEL LQP HVFRSE HQGALWDCLL SFIRLLLNYR KSSRHLAAFI NKFVQFIHKY ITYNAPAAIS FLQKHADPLH DLSFDNSDLV MLKS LLAGL SLPSRDDRTD RGLDEEGEEE SSAGSLPLVS VSLFTPLTAA EMAPYMKRLS RGQTVEDLLE VLSDIDEMSR RRPEI LSFF STNLQRLMSS AEECCRNLAF SLALRSMQNS PSIAAAFLPT FMYCLGSQDF EVVQTALRNL PEYALLCQEH AAVLLH RAF LVGMYGQMDP SAQISEALRI LHMEAVM

UniProtKB: Integrator complex subunit 1

Macromolecule #2: Integrator complex subunit 2

• Macromolecule: Name: Integrator complex subunit 2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 134.451625 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKDQQTVIMT ECTSLQFVSP FAFEAMQKVD VVCLASLSDP ELRLLLPCLV RMALCAPADQ SQSWAQDKKL ILRLLSGVEA VNSIVALLS VDFHALEQDA SKEQQLRHKL GGGSGESILV SQLQHGLTLE FEHSDSPRRL RLVLSELLAI MNKVSESNGE F FFKSSELF ESPVYLEEAA DVLCILQAEL PSLLPIVDVA EALLHVRNGA WFLCLLVANV PDSFNEVCRG LIKNGERQDE ES LGGRRRT DALRFLCKMN PSQALKVRGM VVEECHLPGL GVALTLDHTK NEACEDGVSD LVCFVSGLLL GTNAKVRTWF GTF IRNGQQ RKRETSSSVL WQMRRQLLLE LMGILPTVRS TRIVEEADVD MEPNVSVYSG LKEEHVVKAS ALLRLYCALM GIAG LKPTE EEAEQLLQLM TSRPPATPAG VRFVSLSFCM LLAFSTLVST PEQEQLMVVW LSWMIKEEAY FESTSGVSAS FGEML LLVA MYFHSNQLSA IIDLVCSTLG MKIVIKPSSL SRMKTIFTQE IFTEQVVTAH AVRVPVTSNL SANITGFLPI HCIYQL LRS RSFTKHKVSI KDWIYRQLCE TSTPLHPQLL PLIDVYINSI LTPASKSNPE ATNQPVTEQE ILNIFQGVIG GDNIRLN QR FSITAQLLVL YYILSYEEAL LANTKTLAAM QRKPKSYSSS LMDQIPIKFL IRQAQGLQQE LGGLHSALLR LLATNYPH L CIVDDWICEE EITGTDALLR RMLLTNNAKN HSPKQLQEAF SAVPVNNTQV MQIIEHLTLL SASELIPYAE VLTSNMSQL LNSGVPRRIL QTVNKLWMVL NTVMPRRLWV MTVNALQPSI KFVRQQKYTQ NDLMIDPLIV LRCDQRVHRC PPLMDITLHM LNGYLLASK AYLSAHLKET EQDRPSQNNT IGLVGQTDAP EVTREELKNA LLAAQDSAAV QILLEICLPT EEEKANGVNP D SLLRNVQS VITTSAPNKG MEEGEDNLLC NLREVQCLIC CLLHQMYIAD PNIAKLVHFQ GYPCELLPLT VAGIPSMHIC LD FIPELIA QPELEKQIFA IQLLSHLCIQ YALPKSLSVA RLAVNVMGTL LTVLTQAKRY AFFMPTLPSL VSFCRAFPPL YED IMSLLI QIGQVCASDV ATQTRDIDPI ITRLQQIKEK PSGWSQICKD SSYKNGSRDT GSMDPDVQLC HCIERTVIEI INMS VSGI

UniProtKB: Integrator complex subunit 2

Macromolecule #3: Integrator complex subunit 4

• Macromolecule: Name: Integrator complex subunit 4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 108.306758 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAHLKKRVY EEFTKVVQPQ EEIATKKLRL TKPSKSAALH IDLCKATSPA DALQYLLQFA RKPVEAESVE GVVRILLEHY YKENDPSVR LKIASLLGLL SKTAGFSPDC IMDDAINILQ NEKSHQVLAQ LLDTLLAIGT KLPENQAIQM RLVDVACKHL T DTSHGVRN KCLQLLGNLG SLEKSVTKDA EGLAARDVQK IIGDYFSDQD PRVRTAAIKA MLQLHERGLK LHQTIYNQAC KL LSDDYEQ VRSAAVQLIW VVSQLYPESI VPIPSSNEEI RLVDDAFGKI CHMVSDGSWV VRVQAAKLLG SMEQVSSHFL EQT LDKKLM SDLRRKRTAH ERAKELYSSG EFSSGRKWGD DAPKEEVDTG AVNLIESGAC GAFVHGLEDE MYEVRIAAVE ALCM LAQSS PSFAEKCLDF LVDMFNDEIE EVRLQSIHTM RKISNNITLR EDQLDTVLAV LEDSSRDIRE ALHELLCCTN VSTKE GIHL ALVELLKNLT KYPTDRDSIW KCLKFLGSRH PTLVLPLVPE LLSTHPFFDT AEPDMDDPAY IAVLVLIFNA AKTCPT MPA LFSDHTFRHY AYLRDSLSHL VPALRLPGRK LVSSAVSPSI IPQEDPSQQF LQQSLERVYS LQHLDPQGAQ ELLEFTI RD LQRLGELQSE LAGVADFSAT YLRCQLLLIK ALQEKLWNVA APLYLKQSDL ASAAAKQIME ETYKMEFMYS GVENKQVV I IHHMRLQAKA LQLIVTARTT RGLDPLFGMC EKFLQEVDFF QRYFIADLPH LQDSFVDKLL DLMPRLMTSK PAEVVKILQ TMLRQSAFLH LPLPEQIHKA SATIIEPAGE SDNPLRFTSG LVVALDVDAT LEHVQDPQNT VKVQVLYPDG QAQMIHPKPA DFRNPGPGR HRLITQVYLS HTAWTEACQV EVRLLLAYNS SARIPKCPWM EGGEMSPQVE TSIEGTIPFS KPVKVYIMPK P ARR

UniProtKB: Integrator complex subunit 4

Macromolecule #4: Integrator complex subunit 5

• Macromolecule: Name: Integrator complex subunit 5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 108.115227 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSALCDPPGA PGPPGPAPAT HGPAPLSAQE LSQEIKAFLT GVDPILGHQL SAREHARCGL LLLRSLPPAR AAVLDHLRGV FDESVRAHL AALDETPVAG PPHLRPPPPS HVPAGGPGLE DVVQEVQQVL SEFIRANPKA WAPVISAWSI DLMGQLSSTY S GQHQRVPH ATGALNELLQ LWMGCRATRT LMDIYVQCLS ALIGSCPDAC VDALLDTSVQ HSPHFDWVVA HIGSSFPGTI IS RVLSCGL KDFCVHGGAG GGAGSSGGSS SQTPSTDPFP GSPAIPAEKR VPKIASVVGI LGHLASRHGD SIRRELLRMF HDS LAGGSG GRSGDPSLQA TVPFLLQLAV MSPALLGTVS GELVDCLKPP AVLSQLQQHL QGFPREELDN MLNLAVHLVS QASG AGAYR LLQFLVDTAM PASVITTQGL AVPDTVREAC DRLIQLLLLH LQKLVHHRGG SPGEGVLGPP PPPRLVPFLD ALKNH VGEL CGETLRLERK RFLWQHQLLG LLSVYTRPSC GPEALGHLLS RARSPEELSL ATQLYAGLVV SLSGLLPLAF RSCLAR VHA GTLQPPFTAR FLRNLALLVG WEQQGGEGPA ALGAHFGESA SAHLSDLAPL LLHPEEEVAE AAASLLAICP FPSEALS PS QLLGLVRAGV HRFFASLRLH GPPGVASACQ LLTRLSQTSP AGLKAVLQLL VEGALHRGNT ELFGGQVDGD NETLSVVS A SLASASLLDT NRRHTAAVPG PGGIWSVFHA GVIGRGLKPP KFVQSRNQQE VIYNTQSLLS LLVHCCSAPG GTECGECWG APILSPEAAK AVAVTLVESV CPDAAGAELA WPPEEHARAT VERDLRIGRR FREQPLLFEL LKLVAAAPPA LCYCSVLLRG LLAALLGHW EASRHPDTTH SPWHLEASCT LVAVMAEGSL LPPALGNMHE VFSQLAPFEV RLLLLSVWGF LREHGPLPQK F IFQSERGR FIRDFSREGG GEGGPHLAVL HSVLHRNIDR LGLFSGRFQA PSPSTLLRQG T

UniProtKB: Integrator complex subunit 5

Macromolecule #5: Integrator complex subunit 6

• Macromolecule: Name: Integrator complex subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.527078 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY AIKAGWKENH ATFMNELKNL QAEGLTTLG QSLRTAFDLL NLNRLVTGID NYGQGRNPFF LEPAIIITIT DGSKLTTTSG VQDELHLPLN SPLPGSELTK E PFRWDQRL FALVLRLPGT MSVESEQLTG VPLDDSAITP MCEVTGGRSY SVCSPRMLNQ CLESLVQKVQ SGVVINFEKA GP DPSPVED GQPDISRPFG SQPWHSCHKL IYVRPNPKTG VPIGHWPVPE SFWPDQNSPT LPPRTSHPVV KFSCTDCEPM VID KLPFDK YELEPSPLTQ FILERKSPQT CWQVYVSNSA KYSELGHPFG YLKASTALNC VNLFVMPYNY PVLLPLLDDL FKVH KAKPT LKWRQSFESY LKTMPPYYLG PLKKAVRMMG APNLIADSME YGLSYSVISY LKKLSQQAKI ESDRVIGSVG KKVVQ ETGI KVRSRSHGLS MAYRKDFQQL LQGISEDVPH RLLDLNMKEY TGFQVALLNK DLKPQTFRNA YDIPRRNLLD HLTRMR SNL LKSTRRFLKG QDEDQVHSVP IAQMGNYQEY LKQVPSPLRE LDPDQPRRLH TFGNPFKLDK KGMMIDEADE FVAGPQN KH KRPGEPNMQG IPKRRRCMSP LLRGRQQNPV VNNHIGGKGP PAPTTQAQPD LIKPLPLHKI SETTNDSIIH DVVENHVA D QLSSDITPNA MDTEFSASSP ASLLERPTNH MEALGHDHLG TNDLTVGGFL ENHEEPRDKE QCAEENIPAS SLNKGKKLM HCRSHEEVNT ELKAQIMKEI RKPGRKYERI FTLLKHVQGS LQTRLIFLQN VIKEASRFKK RMLIEQLENF LDEIHRRANQ INHINSN

UniProtKB: Integrator complex subunit 6

Macromolecule #6: Integrator complex subunit 7

• Macromolecule: Name: Integrator complex subunit 7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 106.952617 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MASNSTKSFL ADAGYGEQEL DANSALMELD KGLRSGKLGE QCEAVVRFPR LFQKYPFPIL INSAFLKLAD VFRVGNNFLR LCVLKVTQQ SEKHLEKILN VDEFVKRIFS VIHSNDPVAR AITLRMLGSL ASIIPERKNA HHSIRQSLDS HDNVEVEAAV F AAANFSAQ SKDFAVGICN KISEMIQGLA TPVDLKLKLI PILQHMHHDA ILASSARQLL QQLVTSYPST KMVIVSLHTF TL LAASSLV DTPKQIQLLL QYLKNDPRKA VKRLAIQDLK LLANKTPHTW SRENIQALCE CALQTPYDSL KLGMLSVLST LSG TIAIKH YFSIVPGNVS SSPRSSDLVK LAQECCYHNN RGIAAHGVRV LTNITVSCQE KDLLALEQDA VFGLESLLVL CSQD DSPGA QATLKIALNC MVKLAKGRPH LSQSVVETLL TQLHSAQDAA RILMCHCLAA IAMQLPVLGD GMLGDLMELY KVIGR SATD KQQELLVSLA TVIFVASQKA LSVESKAVIK QQLESVSNGW TVYRIARQAS RMGNHDMAKE LYQSLLTQVA SEHFYF WLN SLKEFSHAEQ CLTGLQEENY SSALSCIAES LKFYHKGIAS LTAASTPLNP LSFQCEFVKL RIDLLQAFSQ LICTCNS LK TSPPPAIATT IAMTLGNDLQ RCGRISNQMK QSMEEFRSLA SRYGDLYQAS FDADSATLRN VELQQQSCLL ISHAIEAL I LDPESASFQE YGSTGTAHAD SEYERRMMSV YNHVLEEVES LNRKYTPVSY MHTACLCNAI IALLKVPLSF QRYFFQKLQ STSIKLALSP SPRNPAEPIA VQNNQQLALK VEGVVQHGSK PGLFRKIQSV CLNVSSTLQS KSGQDYKIPI DNMTNEMEQR VEPHNDYFS TQFLLNFAIL GTHNITVESS VKDANGIVWK TGPRTTIFVK SLEDPYSQQI RLQQQQAQQP LQQQQQRNAY T RF

UniProtKB: Integrator complex subunit 7

Macromolecule #7: Integrator complex subunit 8

• Macromolecule: Name: Integrator complex subunit 8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 113.219859 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSAEAADREA ATSSRPCTPP QTCWFEFLLE ESLLEKHLRK PCPDPAPVQL IVQFLEQASK PSVNEQNQVQ PPPDNKRNRI LKLLALKVA AHLKWDLDIL EKSLSVPVLN MLLNELLCIS KVPPGTKHVD MDLATLPPTT AMAVLLYNRW AIRTIVQSSF P VKQAKPGP PQLSVMNQMQ QEKELTENIL KVLKEQAADS ILVLEAALKL NKDLYVHTMR TLDLLAMEPG MVNGETESST AG LKVKTEE MQCQVCYDLG AAYFQQGSTN SAVYENAREK FFRTKELIAE IGSLSLHCTI DEKRLAGYCQ ACDVLVPSSD STS QQLTPY SQVHICLRSG NYQEVIQIFI EDNLTLSLPV QFRQSVLREL FKKAQQGNEA LDEICFKVCA CNTVRDILEG RTIS VQFNQ LFLRPNKEKI DFLLEVCSRS VNLEKASESL KGNMAAFLKN VCLGLEDLQY VFMISSHELF ITLLKDEERK LLVDQ MRKR SPRVNLCIKP VTSFYDIPAS ASVNIGQLEH QLILSVDPWR IRQILIELHG MTSERQFWTV SNKWEVPSVY SGVILG IKD NLTRDLVYIL MAKGLHCSTV KDFSHAKQLF AACLELVTEF SPKLRQVMLN EMLLLDIHTH EAGTGQAGER PPSDLIS RV RGYLEMRLPD IPLRQVIAEE CVAFMLNWRE NEYLTLQVPA FLLQSNPYVK LGQLLAATCK ELPGPKESRR TAKDLWEV V VQICSVSSQH KRGNDGRVSL IKQRESTLGI MYRSELLSFI KKLREPLVLT IILSLFVKLH NVREDIVNDI TAEHISIWP SSIPNLQSVD FEAVAITVKE LVRYTLSINP NNHSWLIIQA DIYFATNQYS AALHYYLQAG AVCSDFFNKA VPPDVYTDQV IKRMIKCCS LLNCHTQVAI LCQFLREIDY KTAFKSLQEQ NSHDAMDSYY DYIWDVTILE YLTYLHHKRG ETDKRQIAIK A IGQTELNA SNPEEVLQLA AQRRKKKFLQ AMAKLYF

UniProtKB: Integrator complex subunit 8

Macromolecule #8: Integrator complex subunit 9

• Macromolecule: Name: Integrator complex subunit 9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 73.891219 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG NAFLDKELKE CSGHVFVDSV PEFCLPETE LIDLSTVDVI LISNYHCMMA LPYITEHTGF TGTVYATEPT VQIGRLLMEE LVNFIERVPK AQSASLWKNK D IQRLLPSP LKDAVEVSTW RRCYTMQEVN SALSKIQLVG YSQKIELFGA VQVTPLSSGY ALGSSNWIIQ SHYEKVSYVS GS SLLTTHP QPMDQASLKN SDVLVLTGLT QIPTANPDGM VGEFCSNLAL TVRNGGNVLV PCYPSGVIYD LLECLYQYID SAG LSSVPL YFISPVANSS LEFSQIFAEW LCHNKQSKVY LPEPPFPHAE LIQTNKLKHY PSIHGDFSND FRQPCVVFTG HPSL RFGDV VHFMELWGKS SLNTVIFTEP DFSYLEALAP YQPLAMKCIY CPIDTRLNFI QVSKLLKEVQ PLHVVCPEQY TQPPP AQSH RMDLMIDCQP PAMSYRRAEV LALPFKRRYE KIEIMPELAD SLVPMEIKPG ISLATVSAVL HTKDNKHLLQ PPPRPA QPT SGKKRKRVSD DVPDCKVLKP LLSGSIPVEQ FVQTLEKHGF SDIKVEDTAK GHIVLLQEAE TLIQIEEDST HIICDND EM LRVRLRDLVL KFLQKF

UniProtKB: Integrator complex subunit 9

Macromolecule #9: Integrator complex subunit 11

• Macromolecule: Name: Integrator complex subunit 11 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO; EC number: Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 67.756562 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MPEIRVTPLG AGQDVGRSCI LVSIAGKNVM LDCGMHMGFN DDRRFPDFSY ITQNGRLTDF LDCVIISHFH LDHCGALPYF SEMVGYDGP IYMTHPTQAI CPILLEDYRK IAVDKKGEAN FFTSQMIKDC MKKVVAVHLH QTVQVDDELE IKAYYAGHVL G AAMFQIKV GSESVVYTGD YNMTPDRHLG AAWIDKCRPN LLITESTYAT TIRDSKRCRE RDFLKKVHET VERGGKVLIP VF ALGRAQE LCILLETFWE RMNLKVPIYF STGLTEKANH YYKLFIPWTN QKIRKTFVQR NMFEFKHIKA FDRAFADNPG PMV VFATPG MLHAGQSLQI FRKWAGNEKN MVIMPGYCVQ GTVGHKILSG QRKLEMEGRQ VLEVKMQVEY MSFSAHADAK GIMQ LVGQA EPESVLLVHG EAKKMEFLKQ KIEQELRVNC YMPANGETVT LPTSPSIPVG ISLGLLKREM AQGLLPEAKK PRLLH GTLI MKDSNFRLVS SEQALKELGL AEHQLRFTCR VHLHDTRKEQ ETALRVYSHL KSVLKDHCVQ HLPDGSVTVE SVLLQA AAP SEDPGTKVLL VSWTYQDEEL GSFLTSLLKK GLPQAPS

UniProtKB: Integrator complex subunit 11

Macromolecule #10: Unknown2

• Macromolecule: Name: Unknown2 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 1.464797 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #11: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit...

• Macromolecule: Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.378344 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAAADGDDSL YPIAVLIDEL RNEDVQLRLN SIKKLSTIAL ALGVERTRSE LLPFLTDTIY DEDEVLLALA EQLGTFTTLV GGPEYVHCL LPPLESLATV EETVVRDKAV ESLRAISHEH SPSDLEAHFV PLVKRLAGGD WFTSRTSACG LFSVCYPRVS S AVKAELRQ YFRNLCSDDT PMVRRAAASK LGEFAKVLEL DNVKSEIIPM FSNLASDEQD SVRLLAVEAC VNIAQLLPQE DL EALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC REN VIMSQI LPCIKELVSD ANQHVKSALA SVIMGLSPIL GKDNTIEHLL PLFLAQLKDE CPEVRLNIIS NLDCVNEVIG IRQL SQSLL PAIVELAEDA KWRVRLAIIE YMPLLAGQLG VEFFDEKLNS LCMAWLVDHV YAIREAATSN LKKLVEKFGK EWAHA TIIP KVLAMSGDPN YLHRMTTLFC INVLSEVCGQ DITTKHMLPT VLRMAGDPVA NVRFNVAKSL QKIGPILDNS TLQSEV KPI LEKLTQDQDV DVKYFAQEAL TVLSLA

UniProtKB: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Macromolecule #12: Serine/threonine-protein phosphatase 2A catalytic subunit alpha i...

• Macromolecule: Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-serine/threonine phosphatase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 35.636152 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDR GYYSVETVTL LVALKVRYRE RITILRGNHE SRQITQVYGF YDECLRKYGN ANVWKYFTDL FDYLPLTALV D GQIFCLHG GLSPSIDTLD HIRALDRLQE VPHEGPMCDL LWSDPDDRGG WGISPRGAGY TFGQDISETF NHANGLTLVS RA HQLVMEG YNWCHDRNVV TIFSAPNYCY RCGNQAAIME LDDTLKYSFL QFDPAPRRGE PHVTRRTPDY FL

UniProtKB: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Macromolecule #13: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 2.315846 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #14: DNA-directed RNA polymerase II subunit RPB1,DNA-directed RNA poly...

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB1,DNA-directed RNA polymerase II subunit RPB1, CTD1, CTD2, CTD3, CTD4; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 217.220078 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIE LAKPVFHVGF LVKTMKVLRC VCFFCSKLLV DSNNPKIKDI LAKSKGQPKK RLTHVYDLCK GKNICEGGEE M DNKFGVEQ PEGDEDLTKE KGHGGCGRYQ PRIRRSGLEL YAEWKHVNED SQEKKILLSP ERVHEIFKRI SDEECFVLGM EP RYARPEW MIVTVLPVPP LSVRPAVVMQ GSARNQDDLT HKLADIVKIN NQLRRNEQNG AAAHVIAEDV KLLQFHVATM VDN ELPGLP RAMQKSGRPL KSLKQRLKGK EGRVRGNLMG KRVDFSARTV ITPDPNLSID QVGVPRSIAA NMTFAEIVTP FNID RLQEL VRRGNSQYPG AKYIIRDNGD RIDLRFHPKP SDLHLQTGYK VERHMCDGDI VIFNRQPTLH KMSMMGHRVR ILPWS TFRL NLSVTTPYNA DFDGDEMNLH LPQSLETRAE IQELAMVPRM IVTPQSNRPV MGIVQDTLTA VRKFTKRDVF LERGEV MNL LMFLSTWDGK VPQPAILKPR PLWTGKQIFS LIIPGHINCI RTHSTHPDDE DSGPYKHISP GDTKVVVENG ELIMGIL CK KSLGTSAGSL VHISYLEMGH DITRLFYSNI QTVINNWLLI EGHTIGIGDS IADSKTYQDI QNTIKKAKQD VIEVIEKA H NNELEPTPGN TLRQTFENQV NRILNDARDK TGSSAQKSLS EYNNFKSMVV SGAKGSKINI SQVIAVVGQQ NVEGKRIPF GFKHRTLPHF IKDDYGPESR GFVENSYLAG LTPTEFFFHA MGGREGLIDT AVKTAETGYI QRRLIKSMES VMVKYDATVR NSINQVVQL RYGEDGLAGE SVEFQNLATL KPSNKAFEKK FRFDYTNERA LRRTLQEDLV KDVLSNAHIQ NELEREFERM R EDREVLRV IFPTGDSKVV LPCNLLRMIW NAQKIFHINP RLPSDLHPIK VVEGVKELSK KLVIVNGDDP LSRQAQENAT LL FNIHLRS TLCSRRMAEE FRLSGEAFDW LLGEIESKFN QAIAHPGEMV GALAAQSLGE PATQMTLNTF HYAGVSAKNV TLG VPRLKE LINISKKPKT PSLTVFLLGQ SARDAERAKD ILCRLEHTTL RKVTANTAIY YDPNPQSTVV AEDQEWVNVY YEMP DFDVA RISPWLLRVE LDRKHMTDRK LTMEQIAEKI NAGFGDDLNC IFNDDNAEKL VLRIRIMNSD ENKMQEEEEV VDKMD DDVF LRCIESNMLT DMTLQGIEQI SKVYMHLPQT DNKKKIIITE DGEFKALQEW ILETDGVSLM RVLSEKDVDP VRTTSN DIV EIFTVLGIEA VRKALERELY HVISFDGSYV NYRHLALLCD TMTCRGHLMA ITRHGVNRQD TGPLMKCSFE ETVDVLM EA AAHGESDPMK GVSENIMLGQ LAPAGTGCFD LLLDAEKCKY GMEIPTNIPG LGAAGPTGMF FGSAPSPMGG ISPAMTPW N QGATPAYGAW SPSVGSGMTP GAAGFSPSAA SDASGFSPGY SPAWSPTPGS PGSPGPSSPY IPSPGGAMSP SYSPTSPAY EPRSPGGYTP QSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTS PSYSPTSPSY (UNK)(UNK)(UNK)(UNK)PSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPT SPNY SPTSPNYTPT SPSYSPTSPS YSPTSPNYTP TSPNYSPTSP SYSPTSPSYS PTSPSYSPSS PRYTPQSPTY TPSSPS YSP SSPSYSPASP KYTPTSPSYS PSSPEYTPT(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PKY SPTSP KYSP TSPTYSPTTP KYSPTSPTYS PTSPVYTPTS PKYSPTSPTY SPTSPKYSPT SPTYSPTSPK GSTYSPTSPG YSPTSP TYS LTSPAISPDD SDEEN

UniProtKB: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB1

Macromolecule #15: DNA-directed RNA polymerase subunit beta

• Macromolecule: Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 134.041422 KDa

Sequence

String:

MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE DAPPIDLQAE AQHASGEVEE PPRYLLKFE QIYLSKPTHW ERDGAPSPMM PNEARLRNLT YSAPLYVDIT KTVIKEGEEQ LQTQHQKTFI GKIPIMLRST Y CLLNGLTD RDLCELNECP LDPGGYFIIN GSEKVLIAQE KMATNTVYVF AKKDSKYAYT GECRSCLENS SRPTSTIWVS ML ARGGQGA KKSAIGQRIV ATLPYIKQEV PIIIVFRALG FVSDRDILEH IIYDFEDPEM MEMVKPSLDE AFVIQEQNVA LNF IGSRGA KPGVTKEKRI KYAKEVLQKE MLPHVGVSDF CETKKAYFLG YMVHRLLLAA LGRRELDDRD HYGNKRLDLA GPLL AFLFR GMFKNLLKEV RIYAQKFIDR GKDFNLELAI KTRIISDGLK YSLATGNWGD QKKAHQARAG VSQVLNRLTF ASTLS HLRR LNSPIGRDGK LAKPRQLHNT LWGMVCPAET PEGHAVGLVK NLALMAYISV GSQPSPILEF LEEWSMENLE EISPAA IAD ATKIFVNGCW VGIHKDPEQL MNTLRKLRRQ MDIIVSEVSM IRDIREREIR IYTDAGRICR PLLIVEKQKL LLKKRHI DQ LKEREYNNYS WQDLVASGVV EYIDTLEEET VMLAMTPDDL QEKEVAYCST YTHCEIHPSM ILGVCASIIP FPDHNQSP R NTYQSAMGKQ AMGVYITNFH VRMDTLAHVL YYPQKPLVTT RSMEYLRFRE LPAGINSIVA IASYTGYNQE DSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL EGTNRRYTK RDCSTFLRTS ETGIVDQVMV TLNQEGYKFC KIRVRSVRIP QIGDKFASRH GQKGTCGIQY RQEDMPFTCE G ITPDIIIN PHAIPSRMTI GHLIECLQGK VSANKGEIGD ATPFNDAVNV QKISNLLSDY GYHLRGNEVL YNGFTGRKIT SQ IFIGPTY YQRLKHMVDD KIHSRARGPI QILNRQPMEG RSRDGGLRFG EMERDCQIAH GAAQFLRERL FEASDPYQVH VCN LCGIMA IANTRTHTYE CRGCRNKTQI SLVRMPYACK LLFQELMSMS IAPRMMSV

UniProtKB: DNA-directed RNA polymerase subunit beta

Macromolecule #16: DNA-directed RNA polymerase II subunit RPB3

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 30.997557 KDa

Sequence

String:

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV QIDANSSVLH DEFIAHRLGL IPLTSDDIVD KLQYSRDCT CEEFCPECSV EFTLDVRCNE DQTRHVTSRD LISNSPRVIP VTSRNRDNDP SDYVEQDDIL IVKLRKGQEL R LRAYAKKG FGKEHAKWNP TAGVAFEYDP DNALRHTVYP KPEEWPKSEY SELDEDESQA PYDPNGKPER FYYNVESCGS LR PETIVLS ALSGLKKKLS DLQTQLSHEI QSDV

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

Macromolecule #17: DNA-directed RNA polymerase II subunit E

• Macromolecule: Name: DNA-directed RNA polymerase II subunit E / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 24.644318 KDa

Sequence

String:

MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Macromolecule #18: DNA-directed RNA polymerase II subunit F

• Macromolecule: Name: DNA-directed RNA polymerase II subunit F / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 14.477001 KDa

Sequence

String:

MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIISD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

Macromolecule #19: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 17.162273 KDa

Sequence

String:

MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

Macromolecule #20: DNA-directed RNA polymerase II subunit RPB9

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 14.541221 KDa

Sequence

String:

MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH EVDELTQIIA DVSQDPTLPR TEDHPCQKC GHKEAVFFQS HSARAEDAMR LYYVCTAPHC GHRWTE

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

Macromolecule #21: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 7.655123 KDa

Sequence

String:

MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

Macromolecule #22: RNA_pol_L_2 domain-containing protein

• Macromolecule: Name: RNA_pol_L_2 domain-containing protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 13.310284 KDa

Sequence

String:

MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTN AITDLISELS LLEERFRVAI KDKQEGIE

UniProtKB: DNA-directed RNA polymerase II subunit RPB11-a

Macromolecule #23: RPB12

• Macromolecule: Name: RPB12 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 7.018244 KDa

Sequence

String:

MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Macromolecule #27: Negative elongation factor A

• Macromolecule: Name: Negative elongation factor A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.343598 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP V KHFQLKRK PKSATLRAEL LQKSTETAQQ LKRSAGVPFH AKGRGLLRKM DTTTPLKGIP KQAPFRSPTA PSVFSPTGNR TP IPPSRTL LRKERGVKLL DISELDMVGA GREAKRRRKT LDAEVVEKPA KEETVVENAT PDYAAGLVST QKLGSLNNEP ALP STSYLP STPSVVPASS YIPSSETPPA PSSREASRPP EEPSAPSPTL PAQFKQRAPM YNSGLSPATP TPAAPTSPLT PTTP PAVAP TTQTPPVAMV APQTQAPAQQ QPKKNLSLTR EQMFAAQEMF KTANKVTRPE KALILGFMAG SRENPCQEQG DVIQI KLSE HTEDLPKADG QGSTTMLVDT VFEMNYATGQ WTRFKKYKPM TNVS

UniProtKB: Negative elongation factor A

Macromolecule #28: Negative elongation factor B

• Macromolecule: Name: Negative elongation factor B / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.779227 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEER YKKLEDLLEK SFSLVKMPSL QPVVMCVMKH LPKVPEKKLK LVMADKELYR ACAVEVKRQI WQDNQALFGD E VSPLLKQY ILEKESALFS TELSVLHNFF SPSPKTRRQG EVVQRLTRMV GKNVKLYDMV LQFLRTLFLR TRNVHYCTLR AE LLMSLHD LDVGEICTVD PCHKFTWCLD ACIRERFVDS KRARELQGFL DGVKKGQEQV LGDLSMILCD PFAINTLALS TVR HLQELV GQETLPRDSP DLLLLLRLLA LGQGAWDMID SQVFKEPKME VELITRFLPM LMSFLVDDYT FNVDQKLPAE EKAP VSYPN TLPESFTKFL QEQRMACEVG LYYVLHITKQ RNKNALLRLL PGLVETFGDL AFGDIFLHLL TGNLALLADE FALED FCSS LFDGFFLTAS PRKENVHRHA LRLLIHLHPR VAPSKLEALQ KALEPTGQSG EAVKELYSQL GEKLEQLDHR KPSPAQ AAE TPALELPLPS VPAPAPL

UniProtKB: Negative elongation factor B

Macromolecule #29: Negative elongation factor C/D

• Macromolecule: Name: Negative elongation factor C/D / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 66.315352 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MAGAVPGAIM DEDYYGSAAE WGDEADGGQQ EDDSGEGEDD AEVQQECLHK FSTRDYIMEP SIFNTLKRYF QAGGSPENVI QLLSENYTA VAQTVNLLAE WLIQTGVEPV QVQETVENHL KSLLIKHFDP RKADSIFTEE GETPAWLEQM IAHTTWRDLF Y KLAEAHPD CLMLNFTVKL ISDAGYQGEI TSVSTACQQL EVFSRVLRTS LATILDGGEE NLEKNLPEFA KMVCHGEHTY LF AQAMMSV LAQEEQGGSA VRRIAQEVQR FAQEKGHDAS QITLALGTAA SYPRACQALG AMLSKGALNP ADITVLFKMF TSM DPPPVE LIRVPAFLDL FMQSLFKPGA RINQDHKHKY IHILAYAASV VETWKKNKRV SINKDELKST SKAVETVHNL CCNE NKGAS ELVAELSTLY QCIRFPVVAM GVLKWVDWTV SEPRYFQLQT DHTPVHLALL DEISTCHQLL HPQVLQLLVK LFETE HSQL DVMEQLELKK TLLDRMVHLL SRGYVLPVVS YIRKCLEKLD TDISLIRYFV TEVLDVIAPP YTSDFVQLFL PILEND SIA GTIKTEGEHD PVTEFIAHCK SNFIMVN

UniProtKB: Negative elongation factor C/D

Macromolecule #30: Negative elongation factor E

• Macromolecule: Name: Negative elongation factor E / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 43.320922 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRS RTLEGKLKDP EKGPVPTFQP FQRSISADDD LQESSRRPQR KSLYESFVSS SDRLRELGPD GEEAEGPGAG D GPPRSFDW GYEERSGAHS SASPPRSRSR DRSHERNRDR DRDRERDRDR DRDRDRERDR DRDRDRDRDR ERDRDRERDR DR DREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQV ESVQLK VNIARKQPML DAATGKSVWG SLAVQNSPKG CHRDKRTQIV YSDDVYKENL VDGF

UniProtKB: Negative elongation factor E

Macromolecule #31: Transcription elongation factor SPT4

• Macromolecule: Name: Transcription elongation factor SPT4 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.508496 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

GPGSMALETV PKDLRHLRAC LLCSLVKTID QFEYDGCDNC DAYLQMKGNR EMVYDCTSSS FDGIIAMMSP EDSWVSKWQR VSNFKPGVY AVSVTGRLPQ GIVRELKSRG VAYKSRDTAI KT

UniProtKB: Transcription elongation factor SPT4

Macromolecule #32: Transcription elongation factor SPT5

• Macromolecule: Name: Transcription elongation factor SPT5 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 121.145477 KDa
• Recombinant expression: Organism: Homo sapiens (human)

Sequence

String:

MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE EDDDRPPKKP RHGGFILDEA DVDDEYEDE DQWEDGAEDI LEKEEIEASN IDNVVLDEDR SGARRLQNLW RDQREEELGE YYMKKYAKSS VGETVYGGSD E LSDDITQQ QLLPGVKDPN LWTVKCKIGE ERATAISLMR KFIAYQFTDT PLQIKSVVAP EHVKGYIYVE AYKQTHVKQA IE GVGNLRL GYWNQQMVPI KEMTDVLKVV KEVANLKPKS WVRLKRGIYK DDIAQVDYVE PSQNTISLKM IPRIDYDRIK ARM SLKDWF AKRKKFKRPP QRLFDAEKIR SLGGDVASDG DFLIFEGNRY SRKGFLFKSF AMSAVITEGV KPTLSELEKF EDQP EGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG NKITIMPKHE DLKDMLEFPA QELRKYFKMG DHVKV IAGR FEGDTGLIVR VEENFVILFS DLTMHELKVL PRDLQLCSET ASGVDVGGQH EWGELVQLDP QTVGVIVRLE RETFQV LNM YGKVVTVRHQ AVTRKKDNRF AVALDSEQNN IHVKDIVKVI DGPHSGREGE IRHLFRSFAF LHCKKLVENG GMFVCKT RH LVLAGGSKPR DVTNFTVGGF APMSPRISSP MHPSAGGQRG GFGSPGGGSG GMSRGRGRRD NELIGQTVRI SQGPYKGY I GVVKDATEST ARVELHSTCQ TISVDRQRLT TVGSRRPGGM TSTYGRTPMY GSQTPMYGSG SRTPMYGSQT PLQDGSRTP HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF DDEPTPSPQA YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQ FSPYAAPSPQ GSYQPSPSPQ SYHQVAPSPA GYQNTHSPAS YHPTPSPMAY QASPSPSPVG YSPMTPGAPS P GGYNPHTP GSGIEQNSSD WVTTDIQVKV RDTYLDTQVV GQTGVIRSVT GGMCSVYLKD SEKVVSISSE HLEPITPTKN NK VKVILGE DREATGVLLS IDGEDGIVRM DLDEQLKILN LRFLGKLLEA

UniProtKB: Transcription elongation factor SPT5

Macromolecule #33: DNA-directed RNA polymerase II subunit RPB7

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 19.314283 KDa

Sequence

String:

MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVV TQVNKVGLFT EIGPMSCFIS RHSIPSEMEF DPNSNPPCYK TMDEDIVIQQ DDEIRLKIVG TRVDKNDIFA I GSLMDDYL GLVS

UniProtKB: DNA-directed RNA polymerase subunit

Macromolecule #34: DNA-directed RNA polymerase II subunit RPB4

• Macromolecule: Name: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 16.331255 KDa

Sequence

String:

MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQ KKLHKFELAC LANLCPETAE ESKALIPSLE GRFEDEELQQ ILDDIQTKRS FQY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

Macromolecule #24: non-template DNA

• Macromolecule: Name: non-template DNA / type: dna / ID: 24 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 14.932533 KDa

Sequence

String:

(DC)(DC)(DA)(DT)(DT)(DG)(DA)(DG)(DA)(DG) (DC)(DG)(DG)(DC)(DA)(DC)(DT)(DT)(DG)(DT) (DG)(DT)(DT)(DC)(DC)(DG)(DG)(DA)(DG) (DC)(DC)(DA)(DG)(DC)(DA)(DG)(DG)(DG)(DA) (DG) (DC)(DT)(DG)(DG)(DG)(DA)(DG)(DC)

Macromolecule #26: template DNA

• Macromolecule: Name: template DNA / type: dna / ID: 26 / Number of copies: 1 / Classification: DNA
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 14.657324 KDa

Sequence

String:

(DG)(DC)(DT)(DC)(DC)(DC)(DA)(DG)(DC)(DT) (DC)(DC)(DC)(DT)(DG)(DC)(DT)(DG)(DG)(DC) (DT)(DC)(DC)(DG)(DA)(DG)(DT)(DG)(DG) (DG)(DT)(DT)(DC)(DC)(DG)(DC)(DC)(DG)(DC) (DT) (DC)(DT)(DC)(DA)(DA)(DT)(DG)(DG)

Macromolecule #25: RNA

• Macromolecule: Name: RNA / type: rna / ID: 25 / Number of copies: 1
• Source (natural): Organism: synthetic construct (others)
• Molecular weight: Theoretical: 7.432552 KDa

Sequence

String:

AAUAACCGGA GAGGGAACCC ACU

Macromolecule #35: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 35 / Number of copies: 10 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #36: MANGANESE (II) ION

• Macromolecule: Name: MANGANESE (II) ION / type: ligand / ID: 36 / Number of copies: 2 / Formula: MN
• Molecular weight: Theoretical: 54.938 Da

Macromolecule #37: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

7cun

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.18 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 41201
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD