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- EMDB-33691: Structure of the auxin exporter PIN1 in Arabidopsis thaliana in t... -

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Basic information

Entry
Database: EMDB / ID: EMD-33691
TitleStructure of the auxin exporter PIN1 in Arabidopsis thaliana in the apo state
Map dataEM map
Sample
  • Complex: AtPIN1 in complex with a nanobody
    • Complex: AtPIN1
      • Protein or peptide: Auxin efflux carrier component 1
    • Complex: nanobodySingle-domain antibody
      • Protein or peptide: nanobodySingle-domain antibody
Function / homology
Function and homology information


cotyledon morphogenesis / leaf formation / cotyledon development / shoot system development / leaf shaping / xylem and phloem pattern formation / auxin efflux transmembrane transporter activity / inflorescence development / auxin polar transport / gravitropism ...cotyledon morphogenesis / leaf formation / cotyledon development / shoot system development / leaf shaping / xylem and phloem pattern formation / auxin efflux transmembrane transporter activity / inflorescence development / auxin polar transport / gravitropism / plant-type cell wall / photomorphogenesis / root development / flower development / auxin-activated signaling pathway / embryo development ending in seed dormancy / plasmodesma / basal plasma membrane / apical part of cell / plasma membrane / cytoplasm
Similarity search - Function
Membrane transport protein / Auxin efflux carrier, plant type / Membrane transport protein
Similarity search - Domain/homology
Auxin efflux carrier component 1
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress) / Escherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsSun L / Liu X / Yang Z / Xia J
Funding support China, 4 items
OrganizationGrant numberCountry
Chinese Academy of SciencesXDB37020103 China
National Natural Science Foundation of China (NSFC)31900885 and 31870732 China
Other government2008085MC90 and 2008085J15
Other governmentWK9100000031 and YD9100002004 China
CitationJournal: Nature / Year: 2022
Title: Structural insights into auxin recognition and efflux by Arabidopsis PIN1.
Authors: Zhisen Yang / Jing Xia / Jingjing Hong / Chenxi Zhang / Hong Wei / Wei Ying / Chunqiao Sun / Lianghanxiao Sun / Yanbo Mao / Yongxiang Gao / Shutang Tan / Jiří Friml / Dianfan Li / Xin Liu / Linfeng Sun /
Abstract: Polar auxin transport is unique to plants and coordinates their growth and development. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and ...Polar auxin transport is unique to plants and coordinates their growth and development. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and drive polar auxin transport; however, their structures and transport mechanisms remain largely unknown. Here, we report three inward-facing conformation structures of Arabidopsis thaliana PIN1: the apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The transmembrane domain of PIN1 shares a conserved NhaA fold. In the substrate-bound structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding. NPA competes with IAA for the same site at the intracellular pocket, but with a much higher affinity. These findings inform our understanding of the substrate recognition and transport mechanisms of PINs and set up a framework for future research on directional auxin transport, one of the most crucial processes underlying plant development.
History
DepositionJun 26, 2022-
Header (metadata) releaseSep 7, 2022-
Map releaseSep 7, 2022-
UpdateSep 28, 2022-
Current statusSep 28, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_33691.png

Downloads & links

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Map

FileDownload / File: emd_33691.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationEM map
Voxel sizeX=Y=Z: 1.1 Å
Density
Contour LevelBy AUTHOR: 0.6
Minimum - Maximum-3.469643 - 6.425463
Average (Standard dev.)0.004524704 (±0.12237275)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 281.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map 2

Fileemd_33691_half_map_1.map
Annotationhalf map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map 1

Fileemd_33691_half_map_2.map
Annotationhalf map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : AtPIN1 in complex with a nanobody

EntireName: AtPIN1 in complex with a nanobody
Components
  • Complex: AtPIN1 in complex with a nanobody
    • Complex: AtPIN1
      • Protein or peptide: Auxin efflux carrier component 1
    • Complex: nanobodySingle-domain antibody
      • Protein or peptide: nanobodySingle-domain antibody

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Supramolecule #1: AtPIN1 in complex with a nanobody

SupramoleculeName: AtPIN1 in complex with a nanobody / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant cell: HEK293F

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Supramolecule #2: AtPIN1

SupramoleculeName: AtPIN1 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #3: nanobody

SupramoleculeName: nanobody / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2

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Macromolecule #1: Auxin efflux carrier component 1

MacromoleculeName: Auxin efflux carrier component 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Molecular weightTheoretical: 67.080781 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MITAADFYHV MTAMVPLYVA MILAYGSVKW WKIFTPDQCS GINRFVALFA VPLLSFHFIA ANNPYAMNLR FLAADSLQKV IVLSLLFLW CKLSRNGSLD WTITLFSLST LPNTLVMGIP LLKGMYGNFS GDLMVQIVVL QCIIWYTLML FLFEYRGAKL L ISEQFPDT ...String:
MITAADFYHV MTAMVPLYVA MILAYGSVKW WKIFTPDQCS GINRFVALFA VPLLSFHFIA ANNPYAMNLR FLAADSLQKV IVLSLLFLW CKLSRNGSLD WTITLFSLST LPNTLVMGIP LLKGMYGNFS GDLMVQIVVL QCIIWYTLML FLFEYRGAKL L ISEQFPDT AGSIVSIHVD SDIMSLDGRQ PLETEAEIKE DGKLHVTVRR SNASRSDIYS RRSQGLSATP RPSNLTNAEI YS LQSSRNP TPRGSSFNHT DFYSMMASGG GRNSNFGPGE AVFGSKGPTP RPSNYEEDGG PAKPTAAGTA AGAGRFHYQS GGS GGGGGA HYPAPNPGMF SPNTGGGGGT AAKGNAPVVG GKRQDGNGRD LHMFVWSSSA SPVSDVFGGG GGNHHADYST ATND HQKDV KISVPQGNSN DNQYVEREEF SFGNKDDDSK VLATDGGNNI SNKTTQAKVM PPTSVMTRLI LIMVWRKLIR NPNSY SSLF GITWSLISFK WNIEMPALIA KSISILSDAG LGMAMFSLGL FMALNPRIIA CGNRRAAFAA AMRFVVGPAV MLVASY AVG LRGVLLHVAI IQAALPQGIV PFVFAKEYNV HPDILSTAVI FGMLIALPIT LLYYILLGL

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Macromolecule #2: nanobody

MacromoleculeName: nanobody / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.36991 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
GSSSQVQLVE SGGGLVQAGG SLRLSCAASG FPVNISWMEW YRQVPGKERE WVAAIQSTGS YTWYADSVKG RFTISRDNAK NTVYLQMNS LKPEDTAVYY CRVKVGAYYR GQGTQVTVSA GRAG

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DIFFRACTION / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 1.5 µm
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 210597
FSC plot (resolution estimation)

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