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TitleStructural insights into auxin recognition and efflux by Arabidopsis PIN1.
Journal, issue, pagesNature, Vol. 609, Issue 7927, Page 611-615, Year 2022
Publish dateAug 2, 2022
AuthorsZhisen Yang / Jing Xia / Jingjing Hong / Chenxi Zhang / Hong Wei / Wei Ying / Chunqiao Sun / Lianghanxiao Sun / Yanbo Mao / Yongxiang Gao / Shutang Tan / Jiří Friml / Dianfan Li / Xin Liu / Linfeng Sun /
PubMed AbstractPolar auxin transport is unique to plants and coordinates their growth and development. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and ...Polar auxin transport is unique to plants and coordinates their growth and development. The PIN-FORMED (PIN) auxin transporters exhibit highly asymmetrical localizations at the plasma membrane and drive polar auxin transport; however, their structures and transport mechanisms remain largely unknown. Here, we report three inward-facing conformation structures of Arabidopsis thaliana PIN1: the apo state, bound to the natural auxin indole-3-acetic acid (IAA), and in complex with the polar auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). The transmembrane domain of PIN1 shares a conserved NhaA fold. In the substrate-bound structure, IAA is coordinated by both hydrophobic stacking and hydrogen bonding. NPA competes with IAA for the same site at the intracellular pocket, but with a much higher affinity. These findings inform our understanding of the substrate recognition and transport mechanisms of PINs and set up a framework for future research on directional auxin transport, one of the most crucial processes underlying plant development.
External linksNature / PubMed:35917925 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.3 Å
Structure data

33691

7y9t

EMDB-33691, PDB-7y9t:
Structure of the auxin exporter PIN1 in Arabidopsis thaliana in the apo state
Method: EM (single particle) / Resolution: 3.1 Å

33692

7y9u

EMDB-33692, PDB-7y9u:
Structure of the auxin exporter PIN1 in Arabidopsis thaliana in the NPA-bound state
Method: EM (single particle) / Resolution: 3.3 Å

33693

7y9v

EMDB-33693, PDB-7y9v:
Structure of the auxin exporter PIN1 in Arabidopsis thaliana in the IAA-bound state
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-E7O:
2-(naphthalen-1-ylcarbamoyl)benzoic acid

ChemComp-IAC:
1H-INDOL-3-YLACETIC ACID / hormone*YM

ChemComp-HOH:
WATER

Source
  • arabidopsis thaliana (thale cress)
  • escherichia coli (E. coli)
KeywordsTRANSPORT PROTEIN

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