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- EMDB-3303: Cryo-EM reconstruction of Seneca VAlley Virus - receptor complex -

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Basic information

Entry
Database: EMDB / ID: 3303
TitleCryo-EM reconstruction of Seneca VAlley Virus - receptor complex
SampleCryo-EM reconstruction of Seneca Valley Virus - receptor complex
SourceSeneca valley virus / virus
Map dataCryo-EM reconstruction of SVV-receptor complex
Methodsingle particle (icosahedral) reconstruction, at 18 Å resolution
AuthorsMiles LA / Burga L / Bostina M / Poirier JT / Rudin CM
CitationJ. Clin. Invest., 2017, 127, 2957-2967

J. Clin. Invest., 2017, 127, 2957-2967 StrPapers
Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus.
Linde A Miles / Laura N Burga / Eric E Gardner / Mihnea Bostina / John T Poirier / Charles M Rudin

DateDeposition: Jan 21, 2016 / Header (metadata) release: Feb 3, 2016 / Last update: Feb 3, 2016

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Structure visualization

Downloads & links

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Map

FileOn hold (released when the article is published)
Voxel sizeX=Y=Z: 3.12 Å
Density
Contour Level:0.02 (by author)
Minimum - Maximum-0.00622528 - 0.13083661
Average (Standard dev.)0.00792713 (0.02601571)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions180180180
Origin383838
Limit217217217
Spacing180180180
CellA=B=C: 561.6 Å
α=β=γ: 90 deg.

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Supplemental data

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Sample components

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Entire Cryo-EM reconstruction of Seneca Valley Virus - receptor complex

EntireName: Cryo-EM reconstruction of Seneca Valley Virus - receptor complex
Number of components: 2

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Component #1: virus, Seneca valley virus

VirusName: Seneca valley virus / Class: VIRION / Empty: No / Enveloped: No / Isolate: SPECIES
SpeciesSpecies: Seneca valley virus / virus
Source (natural)Host Species: Homo sapiens / human / Host category: VERTEBRATES

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Experimental details

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Sample preparation

Specimen stateparticle
VitrificationInstrument: LEICA KF80 / Cryogen name: ETHANE

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Electron microscopy imaging

ImagingMicroscope: JEOL 2200FS / Date: Oct 1, 2015
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 30 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: JEOL
CameraDetector: TVIPS TEMCAM-F416 (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 400

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Image processing

ProcessingMethod: single particle (icosahedral) reconstruction / Applied symmetry: I (icosahedral) / Number of projections: 400
3D reconstructionSoftware: Relion / Resolution: 18 Å / Resolution method: FSC 0.143, gold-standard

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