Cryo-EM structure of Xenopus laevis nuclear pore complex cytoplasmic ring subunit
マップデータ
試料
複合体: nuclear pore complex cytoplasmic ring
タンパク質・ペプチド: x 15種
キーワード
cytoplasmic ring / cryo-EM / nuclear pore complex / Xenopus laevis / NUCLEAR PROTEIN
機能・相同性
機能・相同性情報
system development / nephron development / GATOR2 complex / Seh1-associated complex / nuclear pore inner ring / animal organ development / protein exit from endoplasmic reticulum / COPII-coated vesicle budding / nuclear pore central transport channel / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery ...system development / nephron development / GATOR2 complex / Seh1-associated complex / nuclear pore inner ring / animal organ development / protein exit from endoplasmic reticulum / COPII-coated vesicle budding / nuclear pore central transport channel / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore complex assembly / nuclear pore outer ring / nuclear pore organization / COPII vesicle coat / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / attachment of mitotic spindle microtubules to kinetochore / nuclear localization sequence binding / structural constituent of nuclear pore / RNA export from nucleus / nucleocytoplasmic transport / poly(A)+ mRNA export from nucleus / mitotic metaphase chromosome alignment / ribosomal large subunit export from nucleus / positive regulation of TOR signaling / mRNA transport / nuclear pore / mRNA export from nucleus / cellular response to nutrient levels / ribosomal small subunit export from nucleus / serine-type peptidase activity / positive regulation of TORC1 signaling / nuclear periphery / GTPase activator activity / cellular response to amino acid starvation / chromosome segregation / phospholipid binding / kinetochore / protein import into nucleus / spindle pole / protein transport / nuclear membrane / lysosomal membrane / cell division / centrosome / positive regulation of DNA-templated transcription / structural molecule activity / proteolysis / DNA binding / zinc ion binding / nucleoplasm / cytosol / cytoplasm 類似検索 - 分子機能
ジャーナル: Protein Cell / 年: 2022 タイトル: 8 Å structure of the outer rings of the Xenopus laevis nuclear pore complex obtained by cryo-EM and AI. 著者: Linhua Tai / Yun Zhu / He Ren / Xiaojun Huang / Chuanmao Zhang / Fei Sun / 要旨: The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo- ...The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo-electron microscopic (cryo-EM) structure of the outer rings containing nuclear ring (NR) and cytoplasmic ring (CR) from the Xenopus laevis NPC, with local resolutions reaching 4.9 Å. With the aid of AlphaFold2, we managed to build a pseudoatomic model of the outer rings, including the Y complexes and flanking components. In this most comprehensive and accurate model of outer rings to date, the almost complete Y complex structure exhibits much tighter interaction in the hub region. In addition to two copies of Y complexes, each asymmetric subunit in CR contains five copies of Nup358, two copies of the Nup214 complex, two copies of Nup205 and one copy of newly identified Nup93, while that in NR contains one copy of Nup205, one copy of ELYS and one copy of Nup93. These in-depth structural features represent a great advance in understanding the assembly of NPCs.