Journal: Proc Natl Acad Sci U S A / Year: 2022 Title: Annealing synchronizes the 70 ribosome into a minimum-energy conformation. Authors: Xiaofeng Chu / Xin Su / Mingdong Liu / Li Li / Tianhao Li / Yicheng Qin / Guoliang Lu / Lei Qi / Yunhui Liu / Jinzhong Lin / Qing-Tao Shen / Abstract: Researchers commonly anneal metals, alloys, and semiconductors to repair defects and improve microstructures via recrystallization. Theoretical studies indicate that simulated annealing on biological ...Researchers commonly anneal metals, alloys, and semiconductors to repair defects and improve microstructures via recrystallization. Theoretical studies indicate that simulated annealing on biological macromolecules helps predict the final structures with minimum free energy. Experimental validation of this homogenizing effect and further exploration of its applications are fascinating scientific questions that remain elusive. Here, we chose the apo-state 70 ribosome from as a model, wherein the 30 subunit undergoes a thermally driven intersubunit rotation and exhibits substantial structural flexibility as well as distinct free energy. We experimentally demonstrate that annealing at a fast cooling rate enhances the 70 ribosome homogeneity and improves local resolution on the 30 subunit. After annealing, the 70 ribosome is in a nonrotated state with respect to corresponding intermediate structures in unannealed or heated ribosomes. Manifold-based analysis further indicates that the annealed 70 ribosome takes a narrow conformational distribution and exhibits a minimum-energy state in the free-energy landscape. Our experimental results offer a facile yet robust approach to enhance protein stability, which is ideal for high-resolution cryogenic electron microscopy. Beyond structure determination, annealing shows great potential for synchronizing proteins on a single-molecule level and can be extended to study protein folding and explore conformational and energy landscapes.
History
Deposition
May 8, 2021
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Header (metadata) release
Feb 23, 2022
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Map release
Feb 23, 2022
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Update
Mar 16, 2022
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Current status
Mar 16, 2022
Processing site: PDBj / Status: Released
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